Investigating the impact of linseed supplementation, rich in omega-3 fatty acids, on the expression of immune genes in Beetal Goats
Anim Biotechnol. 2023 Oct 31:1-6. doi: 10.1080/10495398.2023.2263049. Online ahead of print.ABSTRACTThe present study investigated the effect of linseed supplementation on serum profiling and differential expression levels of IL-2 and TLR 2 genes involved in the immune system of lactating goats. The study was conducted on 15 lactating Beetal goats for 3 months, which were divided into three groups. Treatment group T1 was kept as a control group with no linseed supplementation, whereas treatment group T2 received ground linseed at the rate 10% of concentrate per day per animal, and treatment group T3 received 20% ground lin...
Source: Animal Biotechnology - October 31, 2023 Category: Biotechnology Authors: Jyoti Shunthwal Zile Singh Sihag Ankit Magotra None Meetu Sajjan Sihag Source Type: research

Gut microbiota absence and transplantation affect diarrhea: an investigation in the germ-free piglet model
Anim Biotechnol. 2023 Oct 31:1-7. doi: 10.1080/10495398.2023.2248200. Online ahead of print.ABSTRACTThis experiment was conducted to explore the effects of gut microbiota on neonatal diarrhea in a germ-free (GF) pig model. Twelve hysterectomy-derived GF piglets were housed in six sterile isolators. Among them, six piglets were treated as the GF group, and the other six piglets were orally introduced with healthy sow fecal suspension and regarded as the fecal microbiota transplantation (FMT) group. Another six piglets from natural birth were considered as the conventional (CV) group. The GF and FMT piglets were hand-fed wit...
Source: Animal Biotechnology - October 31, 2023 Category: Biotechnology Authors: Hua Zhou Bing Yu Jing Sun Hong Chen Zuohua Liu Liangpeng Ge Daiwen Chen Source Type: research

Functional analysis of the < em > GPAT4 < /em > gene mutation predicted to affect splicing
Anim Biotechnol. 2023 Oct 31:1-9. doi: 10.1080/10495398.2023.2269210. Online ahead of print.ABSTRACTThe GPAT4 gene is considered as a potential functional candidate for single nucleotide polymorphism (SNP) studies in dairy cattle breeding due to its association with dairy performance in cattle by encoding an enzyme responsible for the presence of diacylglycerols and triacylglycerols in milk. Using the example of the GPAT4 gene, we applied the minigene splicing assay to analyze the functional consequences of its variant that was predicted to affect normal splicing. The results of functional analysis revealed the sequence va...
Source: Animal Biotechnology - October 31, 2023 Category: Biotechnology Authors: Sergey A Bursakov Anastasia V Kovaleva Artyom V Brigida Oleg G Zaripov Source Type: research

Supplementation with grape seed extract, onion peel extract, or rosemary extract in the diet  alleviates growth inhibition, liver damage, and oxidative stress induced by diquat in Lohmann chicks
Anim Biotechnol. 2023 Oct 25:1-8. doi: 10.1080/10495398.2023.2271532. Online ahead of print.ABSTRACTThe present study aimed to assess the impact of grape seed extract (GSE), onion peel extract (OPE), and rosemary extract (ROE) on Diquat-induced growth restriction and oxidative stress in Lohmann chicks. A total of 200 chicks were randomly assigned to 5 diets: the positive control (PC) group, the negative control (NC) group, GSE group, OPE group, and ROE group. During the first 7 d of trial, compared with NC and PC groups, the GSE group enhanced average daily feed intake (ADFI). From day 8-21, diquat injection resulted in re...
Source: Animal Biotechnology - October 25, 2023 Category: Biotechnology Authors: Man Wang Zongze He Zhaolong Xiong Hongwei Liu Xiang Zhou Jian He Source Type: research

Supplementation with grape seed extract, onion peel extract, or rosemary extract in the diet  alleviates growth inhibition, liver damage, and oxidative stress induced by diquat in Lohmann chicks
Anim Biotechnol. 2023 Oct 25:1-8. doi: 10.1080/10495398.2023.2271532. Online ahead of print.ABSTRACTThe present study aimed to assess the impact of grape seed extract (GSE), onion peel extract (OPE), and rosemary extract (ROE) on Diquat-induced growth restriction and oxidative stress in Lohmann chicks. A total of 200 chicks were randomly assigned to 5 diets: the positive control (PC) group, the negative control (NC) group, GSE group, OPE group, and ROE group. During the first 7 d of trial, compared with NC and PC groups, the GSE group enhanced average daily feed intake (ADFI). From day 8-21, diquat injection resulted in re...
Source: Animal Biotechnology - October 25, 2023 Category: Biotechnology Authors: Man Wang Zongze He Zhaolong Xiong Hongwei Liu Xiang Zhou Jian He Source Type: research

Supplementation with grape seed extract, onion peel extract, or rosemary extract in the diet  alleviates growth inhibition, liver damage, and oxidative stress induced by diquat in Lohmann chicks
Anim Biotechnol. 2023 Oct 25:1-8. doi: 10.1080/10495398.2023.2271532. Online ahead of print.ABSTRACTThe present study aimed to assess the impact of grape seed extract (GSE), onion peel extract (OPE), and rosemary extract (ROE) on Diquat-induced growth restriction and oxidative stress in Lohmann chicks. A total of 200 chicks were randomly assigned to 5 diets: the positive control (PC) group, the negative control (NC) group, GSE group, OPE group, and ROE group. During the first 7 d of trial, compared with NC and PC groups, the GSE group enhanced average daily feed intake (ADFI). From day 8-21, diquat injection resulted in re...
Source: Animal Biotechnology - October 25, 2023 Category: Biotechnology Authors: Man Wang Zongze He Zhaolong Xiong Hongwei Liu Xiang Zhou Jian He Source Type: research

Supplementation with grape seed extract, onion peel extract, or rosemary extract in the diet  alleviates growth inhibition, liver damage, and oxidative stress induced by diquat in Lohmann chicks
Anim Biotechnol. 2023 Oct 25:1-8. doi: 10.1080/10495398.2023.2271532. Online ahead of print.ABSTRACTThe present study aimed to assess the impact of grape seed extract (GSE), onion peel extract (OPE), and rosemary extract (ROE) on Diquat-induced growth restriction and oxidative stress in Lohmann chicks. A total of 200 chicks were randomly assigned to 5 diets: the positive control (PC) group, the negative control (NC) group, GSE group, OPE group, and ROE group. During the first 7 d of trial, compared with NC and PC groups, the GSE group enhanced average daily feed intake (ADFI). From day 8-21, diquat injection resulted in re...
Source: Animal Biotechnology - October 25, 2023 Category: Biotechnology Authors: Man Wang Zongze He Zhaolong Xiong Hongwei Liu Xiang Zhou Jian He Source Type: research

Supplementation with grape seed extract, onion peel extract, or rosemary extract in the diet  alleviates growth inhibition, liver damage, and oxidative stress induced by diquat in Lohmann chicks
Anim Biotechnol. 2023 Oct 25:1-8. doi: 10.1080/10495398.2023.2271532. Online ahead of print.ABSTRACTThe present study aimed to assess the impact of grape seed extract (GSE), onion peel extract (OPE), and rosemary extract (ROE) on Diquat-induced growth restriction and oxidative stress in Lohmann chicks. A total of 200 chicks were randomly assigned to 5 diets: the positive control (PC) group, the negative control (NC) group, GSE group, OPE group, and ROE group. During the first 7 d of trial, compared with NC and PC groups, the GSE group enhanced average daily feed intake (ADFI). From day 8-21, diquat injection resulted in re...
Source: Animal Biotechnology - October 25, 2023 Category: Biotechnology Authors: Man Wang Zongze He Zhaolong Xiong Hongwei Liu Xiang Zhou Jian He Source Type: research

Electroporation-based CRISPR gene editing in adult buffalo fibroblast cells
In conclusion, electroporation conditions reported here can edit buffalo gene(s) for various biotechnological research applications.PMID:37870061 | DOI:10.1080/10495398.2023.2271030 (Source: Animal Biotechnology)
Source: Animal Biotechnology - October 23, 2023 Category: Biotechnology Authors: Kamlesh Kumari Bajwa Meeti Punetha Dharmendra Kumar Prem Singh Yadav Chares R Long Naresh L Selokar Source Type: research

A novel 22-bp InDel within < em > FGF7 < /em > gene is significantly associated with growth traits in goat
Anim Biotechnol. 2023 Oct 23:1-9. doi: 10.1080/10495398.2023.2262537. Online ahead of print.ABSTRACTFibroblast growth factor 7 (FGF7) is involved in lipid metabolism, which is considered as a candidate gene with close relation with muscle development by eGWAs and RNA-Seq analyses. To date, limited research has been conducted on the relationship between FGF7 gene and growth traits. The main objective of this work was to further investigate the association between novel InDel within FGF7 gene and growth traits in goat. Herein, FGF7 mRNA expression levels were investigated in various Fuqing goat tissues. We found that FGF7 ge...
Source: Animal Biotechnology - October 23, 2023 Category: Biotechnology Authors: Xian-Feng Wu Yuan Liu Ying-Gang Wang Fu Zhang Wen-Yang Li Source Type: research

Electroporation-based CRISPR gene editing in adult buffalo fibroblast cells
In conclusion, electroporation conditions reported here can edit buffalo gene(s) for various biotechnological research applications.PMID:37870061 | DOI:10.1080/10495398.2023.2271030 (Source: Animal Biotechnology)
Source: Animal Biotechnology - October 23, 2023 Category: Biotechnology Authors: Kamlesh Kumari Bajwa Meeti Punetha Dharmendra Kumar Prem Singh Yadav Chares R Long Naresh L Selokar Source Type: research

A novel 22-bp InDel within < em > FGF7 < /em > gene is significantly associated with growth traits in goat
Anim Biotechnol. 2023 Oct 23:1-9. doi: 10.1080/10495398.2023.2262537. Online ahead of print.ABSTRACTFibroblast growth factor 7 (FGF7) is involved in lipid metabolism, which is considered as a candidate gene with close relation with muscle development by eGWAs and RNA-Seq analyses. To date, limited research has been conducted on the relationship between FGF7 gene and growth traits. The main objective of this work was to further investigate the association between novel InDel within FGF7 gene and growth traits in goat. Herein, FGF7 mRNA expression levels were investigated in various Fuqing goat tissues. We found that FGF7 ge...
Source: Animal Biotechnology - October 23, 2023 Category: Biotechnology Authors: Xian-Feng Wu Yuan Liu Ying-Gang Wang Fu Zhang Wen-Yang Li Source Type: research

Electroporation-based CRISPR gene editing in adult buffalo fibroblast cells
In conclusion, electroporation conditions reported here can edit buffalo gene(s) for various biotechnological research applications.PMID:37870061 | DOI:10.1080/10495398.2023.2271030 (Source: Animal Biotechnology)
Source: Animal Biotechnology - October 23, 2023 Category: Biotechnology Authors: Kamlesh Kumari Bajwa Meeti Punetha Dharmendra Kumar Prem Singh Yadav Chares R Long Naresh L Selokar Source Type: research

A novel 22-bp InDel within < em > FGF7 < /em > gene is significantly associated with growth traits in goat
Anim Biotechnol. 2023 Oct 23:1-9. doi: 10.1080/10495398.2023.2262537. Online ahead of print.ABSTRACTFibroblast growth factor 7 (FGF7) is involved in lipid metabolism, which is considered as a candidate gene with close relation with muscle development by eGWAs and RNA-Seq analyses. To date, limited research has been conducted on the relationship between FGF7 gene and growth traits. The main objective of this work was to further investigate the association between novel InDel within FGF7 gene and growth traits in goat. Herein, FGF7 mRNA expression levels were investigated in various Fuqing goat tissues. We found that FGF7 ge...
Source: Animal Biotechnology - October 23, 2023 Category: Biotechnology Authors: Xian-Feng Wu Yuan Liu Ying-Gang Wang Fu Zhang Wen-Yang Li Source Type: research

Preliminary investigation reveals novel pathological consequences of bluetongue virus-1 infection in the endocrine glands of pregnant Indian sheep
Anim Biotechnol. 2023 Oct 18:1-13. doi: 10.1080/10495398.2023.2269428. Online ahead of print.ABSTRACTBluetongue virus (BTV), a major peril to the sheep industry, infects a wide range of the cells in the infected animals including mononuclear, dendritic and epithelial cells. However, little is known about its tropism for the secretory epithelial cells of endocrine glands and the pathogenesis it induces. The aim of the study was to assess the BTV load, antigen distribution in the tissue of the pituitary, thyroid as well as adrenal glands and associated histopathological consequences. BTV antigens were localized using immunoh...
Source: Animal Biotechnology - October 18, 2023 Category: Biotechnology Authors: Rohit Singh Karam Pal Singh Rajendra Singh Vidya Singh Pawan Kumar Rajat Varshney Akanksha Yadav Akash Mote Mukesh Gangwar N Babu Prasath Source Type: research