Candidate circRNAs related to skeletal muscle development in Dazu black goats
In this study, the expression patterns of circRNAs in the longissimus dorsi muscle at embryonic day 75 and postnatal day 1 in DBGs were investigated to identify the key circRNAs that play an important role in SMD in goats. A total of 140 significantly and differentially expressed circRNAs (DEcircRNAs) were identified among the groups at different developmental stages. Among the 116 host genes (HGs) of DEcircRNAs, 76 were significantly and differentially expressed, which was confirmed by previous RNA_seq data. Furthermore, the expression pattern of 10 DEcircRNAs with RT-qPCR was verified, which showed 80% concordance rate w...
Source: Animal Biotechnology - November 30, 2023 Category: Biotechnology Authors: Chengli Liu Pu Yang Xiao Wang Baiju Xiang Guangxin E Yongfu Huang Source Type: research
Candidate circRNAs related to skeletal muscle development in Dazu black goats
In this study, the expression patterns of circRNAs in the longissimus dorsi muscle at embryonic day 75 and postnatal day 1 in DBGs were investigated to identify the key circRNAs that play an important role in SMD in goats. A total of 140 significantly and differentially expressed circRNAs (DEcircRNAs) were identified among the groups at different developmental stages. Among the 116 host genes (HGs) of DEcircRNAs, 76 were significantly and differentially expressed, which was confirmed by previous RNA_seq data. Furthermore, the expression pattern of 10 DEcircRNAs with RT-qPCR was verified, which showed 80% concordance rate w...
Source: Animal Biotechnology - November 30, 2023 Category: Biotechnology Authors: Chengli Liu Pu Yang Xiao Wang Baiju Xiang Guangxin E Yongfu Huang Source Type: research
Candidate circRNAs related to skeletal muscle development in Dazu black goats
In this study, the expression patterns of circRNAs in the longissimus dorsi muscle at embryonic day 75 and postnatal day 1 in DBGs were investigated to identify the key circRNAs that play an important role in SMD in goats. A total of 140 significantly and differentially expressed circRNAs (DEcircRNAs) were identified among the groups at different developmental stages. Among the 116 host genes (HGs) of DEcircRNAs, 76 were significantly and differentially expressed, which was confirmed by previous RNA_seq data. Furthermore, the expression pattern of 10 DEcircRNAs with RT-qPCR was verified, which showed 80% concordance rate w...
Source: Animal Biotechnology - November 30, 2023 Category: Biotechnology Authors: Chengli Liu Pu Yang Xiao Wang Baiju Xiang Guangxin E Yongfu Huang Source Type: research
Candidate circRNAs related to skeletal muscle development in Dazu black goats
In this study, the expression patterns of circRNAs in the longissimus dorsi muscle at embryonic day 75 and postnatal day 1 in DBGs were investigated to identify the key circRNAs that play an important role in SMD in goats. A total of 140 significantly and differentially expressed circRNAs (DEcircRNAs) were identified among the groups at different developmental stages. Among the 116 host genes (HGs) of DEcircRNAs, 76 were significantly and differentially expressed, which was confirmed by previous RNA_seq data. Furthermore, the expression pattern of 10 DEcircRNAs with RT-qPCR was verified, which showed 80% concordance rate w...
Source: Animal Biotechnology - November 30, 2023 Category: Biotechnology Authors: Chengli Liu Pu Yang Xiao Wang Baiju Xiang Guangxin E Yongfu Huang Source Type: research
Candidate circRNAs related to skeletal muscle development in Dazu black goats
In this study, the expression patterns of circRNAs in the longissimus dorsi muscle at embryonic day 75 and postnatal day 1 in DBGs were investigated to identify the key circRNAs that play an important role in SMD in goats. A total of 140 significantly and differentially expressed circRNAs (DEcircRNAs) were identified among the groups at different developmental stages. Among the 116 host genes (HGs) of DEcircRNAs, 76 were significantly and differentially expressed, which was confirmed by previous RNA_seq data. Furthermore, the expression pattern of 10 DEcircRNAs with RT-qPCR was verified, which showed 80% concordance rate w...
Source: Animal Biotechnology - November 30, 2023 Category: Biotechnology Authors: Chengli Liu Pu Yang Xiao Wang Baiju Xiang Guangxin E Yongfu Huang Source Type: research
Candidate circRNAs related to skeletal muscle development in Dazu black goats
In this study, the expression patterns of circRNAs in the longissimus dorsi muscle at embryonic day 75 and postnatal day 1 in DBGs were investigated to identify the key circRNAs that play an important role in SMD in goats. A total of 140 significantly and differentially expressed circRNAs (DEcircRNAs) were identified among the groups at different developmental stages. Among the 116 host genes (HGs) of DEcircRNAs, 76 were significantly and differentially expressed, which was confirmed by previous RNA_seq data. Furthermore, the expression pattern of 10 DEcircRNAs with RT-qPCR was verified, which showed 80% concordance rate w...
Source: Animal Biotechnology - November 30, 2023 Category: Biotechnology Authors: Chengli Liu Pu Yang Xiao Wang Baiju Xiang Guangxin E Yongfu Huang Source Type: research
Whole genome resequencing revealed genomic variants and functional pathways related to adaptation in Indian yak populations
This study provides comprehensive information about genomic variants and their annotation in Indian yak populations, thus would serve as a data resource for researchers working on the yaks. Furthermore, it could be well exploited for better yak conservation strategies by estimating population genetics parameters viz., effective population size, inbreeding, and observed and expected heterozygosity.PMID:38006247 | DOI:10.1080/10495398.2023.2282723 (Source: Animal Biotechnology)
Source: Animal Biotechnology - November 25, 2023 Category: Biotechnology Authors: Amod Kumar Mahesh Dige Saket Kumar Niranjan Sonika Ahlawat Reena Arora Aneet Kour Ramesh Kumar Vijh Source Type: research
Comparative transcriptome profile analysis of granulosa cells from porcine ovarian follicles during early atresia
In this study, we isolated the granulosa cells from porcine ovarian follicles (3-5 mm diameter) divided into healthy follicles (HFs) and early atretic follicles (EAFs). We applied high-throughput RNA sequencing to identify and compare differentially expressed genes (DEGs) between HFs and EAFs. A total of 31,694 genes were detected, of which 21,806 were co-expressed in six samples, and 243 genes (p < 0.05; FDR < 0.05) were differentially expressed (DEGs), including 123 downregulated and 120 upregulated in EAFs. GO analysis highlighted hormone metabolism, plasma membrane localization, and transporter activity. The path...
Source: Animal Biotechnology - November 25, 2023 Category: Biotechnology Authors: Jinbi Zhang Xinxin Qin Caixia Wang Yuge Zhang Yizhe Dou Shiyong Xu Jingge Liu Zengxiang Pan Source Type: research
Analysis of OCT4 and PGP9.5 gene expression in prenatal and postnatal buffalo ( < em > Bubalus bubalis < /em > ) testes
This study aimed to investigate and characterize the spermatogonial stem cells (SSCs) in buffaloes at different stages of development, including prenatal, neonatal, prepubertal, and adult testes. We sought a comprehensive understanding of these cells through a combination of histological, immunohistochemical, and ultrastructural analyses. Specifically, we examined changes in the expression of two potential SSC markers, OCT4 and PGP9.5, using immunohistochemistry. Additionally, we conducted a real-time quantitative polymerase chain reaction (RT-qPCR) to assess the relative gene expression of OCT4 and PGP9.5. The relative ex...
Source: Animal Biotechnology - November 25, 2023 Category: Biotechnology Authors: Ashritha Q Carlo Devendra Pathak Ratan K Choudhary Opinder Singh Neelam Bansal Source Type: research
< em > Orychophragmus violaceus < /em > and/or chicory forage affects performance, egg quality, sensory evaluation and antioxidative properties in native laying hens
In conclusion, the present study suggests that CC had a better effect on the performance of the native laying hens than OV. The OV and CC affected egg quality, while YC was increased in CC and OVC groups. The OVC improved YC and serum antioxidative properties of native laying hens without affecting the performance.PMID:38006583 | DOI:10.1080/10495398.2023.2286610 (Source: Animal Biotechnology)
Source: Animal Biotechnology - November 25, 2023 Category: Biotechnology Authors: W Y Zhao Q Q Zhang Y F Zhao C Chang X Wang A L Geng Source Type: research
Whole genome resequencing revealed genomic variants and functional pathways related to adaptation in Indian yak populations
This study provides comprehensive information about genomic variants and their annotation in Indian yak populations, thus would serve as a data resource for researchers working on the yaks. Furthermore, it could be well exploited for better yak conservation strategies by estimating population genetics parameters viz., effective population size, inbreeding, and observed and expected heterozygosity.PMID:38006247 | DOI:10.1080/10495398.2023.2282723 (Source: Animal Biotechnology)
Source: Animal Biotechnology - November 25, 2023 Category: Biotechnology Authors: Amod Kumar Mahesh Dige Saket Kumar Niranjan Sonika Ahlawat Reena Arora Aneet Kour Ramesh Kumar Vijh Source Type: research
Comparative transcriptome profile analysis of granulosa cells from porcine ovarian follicles during early atresia
In this study, we isolated the granulosa cells from porcine ovarian follicles (3-5 mm diameter) divided into healthy follicles (HFs) and early atretic follicles (EAFs). We applied high-throughput RNA sequencing to identify and compare differentially expressed genes (DEGs) between HFs and EAFs. A total of 31,694 genes were detected, of which 21,806 were co-expressed in six samples, and 243 genes (p < 0.05; FDR < 0.05) were differentially expressed (DEGs), including 123 downregulated and 120 upregulated in EAFs. GO analysis highlighted hormone metabolism, plasma membrane localization, and transporter activity. The path...
Source: Animal Biotechnology - November 25, 2023 Category: Biotechnology Authors: Jinbi Zhang Xinxin Qin Caixia Wang Yuge Zhang Yizhe Dou Shiyong Xu Jingge Liu Zengxiang Pan Source Type: research
Analysis of OCT4 and PGP9.5 gene expression in prenatal and postnatal buffalo ( < em > Bubalus bubalis < /em > ) testes
This study aimed to investigate and characterize the spermatogonial stem cells (SSCs) in buffaloes at different stages of development, including prenatal, neonatal, prepubertal, and adult testes. We sought a comprehensive understanding of these cells through a combination of histological, immunohistochemical, and ultrastructural analyses. Specifically, we examined changes in the expression of two potential SSC markers, OCT4 and PGP9.5, using immunohistochemistry. Additionally, we conducted a real-time quantitative polymerase chain reaction (RT-qPCR) to assess the relative gene expression of OCT4 and PGP9.5. The relative ex...
Source: Animal Biotechnology - November 25, 2023 Category: Biotechnology Authors: Ashritha Q Carlo Devendra Pathak Ratan K Choudhary Opinder Singh Neelam Bansal Source Type: research
< em > Orychophragmus violaceus < /em > and/or chicory forage affects performance, egg quality, sensory evaluation and antioxidative properties in native laying hens
In conclusion, the present study suggests that CC had a better effect on the performance of the native laying hens than OV. The OV and CC affected egg quality, while YC was increased in CC and OVC groups. The OVC improved YC and serum antioxidative properties of native laying hens without affecting the performance.PMID:38006583 | DOI:10.1080/10495398.2023.2286610 (Source: Animal Biotechnology)
Source: Animal Biotechnology - November 25, 2023 Category: Biotechnology Authors: W Y Zhao Q Q Zhang Y F Zhao C Chang X Wang A L Geng Source Type: research
Whole genome resequencing revealed genomic variants and functional pathways related to adaptation in Indian yak populations
This study provides comprehensive information about genomic variants and their annotation in Indian yak populations, thus would serve as a data resource for researchers working on the yaks. Furthermore, it could be well exploited for better yak conservation strategies by estimating population genetics parameters viz., effective population size, inbreeding, and observed and expected heterozygosity.PMID:38006247 | DOI:10.1080/10495398.2023.2282723 (Source: Animal Biotechnology)
Source: Animal Biotechnology - November 25, 2023 Category: Biotechnology Authors: Amod Kumar Mahesh Dige Saket Kumar Niranjan Sonika Ahlawat Reena Arora Aneet Kour Ramesh Kumar Vijh Source Type: research
