Investigating the impact of linseed supplementation, rich in omega-3 fatty acids, on the expression of immune genes in Beetal Goats
Anim Biotechnol. 2023 Oct 31:1-6. doi: 10.1080/10495398.2023.2263049. Online ahead of print.ABSTRACTThe present study investigated the effect of linseed supplementation on serum profiling and differential expression levels of IL-2 and TLR 2 genes involved in the immune system of lactating goats. The study was conducted on 15 lactating Beetal goats for 3 months, which were divided into three groups. Treatment group T1 was kept as a control group with no linseed supplementation, whereas treatment group T2 received ground linseed at the rate 10% of concentrate per day per animal, and treatment group T3 received 20% ground lin...
Source: Animal Biotechnology - October 31, 2023 Category: Biotechnology Authors: Jyoti Shunthwal Zile Singh Sihag Ankit Magotra None Meetu Sajjan Sihag Source Type: research

Gut microbiota absence and transplantation affect diarrhea: an investigation in the germ-free piglet model
Anim Biotechnol. 2023 Oct 31:1-7. doi: 10.1080/10495398.2023.2248200. Online ahead of print.ABSTRACTThis experiment was conducted to explore the effects of gut microbiota on neonatal diarrhea in a germ-free (GF) pig model. Twelve hysterectomy-derived GF piglets were housed in six sterile isolators. Among them, six piglets were treated as the GF group, and the other six piglets were orally introduced with healthy sow fecal suspension and regarded as the fecal microbiota transplantation (FMT) group. Another six piglets from natural birth were considered as the conventional (CV) group. The GF and FMT piglets were hand-fed wit...
Source: Animal Biotechnology - October 31, 2023 Category: Biotechnology Authors: Hua Zhou Bing Yu Jing Sun Hong Chen Zuohua Liu Liangpeng Ge Daiwen Chen Source Type: research

Functional analysis of the < em > GPAT4 < /em > gene mutation predicted to affect splicing
Anim Biotechnol. 2023 Oct 31:1-9. doi: 10.1080/10495398.2023.2269210. Online ahead of print.ABSTRACTThe GPAT4 gene is considered as a potential functional candidate for single nucleotide polymorphism (SNP) studies in dairy cattle breeding due to its association with dairy performance in cattle by encoding an enzyme responsible for the presence of diacylglycerols and triacylglycerols in milk. Using the example of the GPAT4 gene, we applied the minigene splicing assay to analyze the functional consequences of its variant that was predicted to affect normal splicing. The results of functional analysis revealed the sequence va...
Source: Animal Biotechnology - October 31, 2023 Category: Biotechnology Authors: Sergey A Bursakov Anastasia V Kovaleva Artyom V Brigida Oleg G Zaripov Source Type: research

Investigating the impact of linseed supplementation, rich in omega-3 fatty acids, on the expression of immune genes in Beetal Goats
Anim Biotechnol. 2023 Oct 31:1-6. doi: 10.1080/10495398.2023.2263049. Online ahead of print.ABSTRACTThe present study investigated the effect of linseed supplementation on serum profiling and differential expression levels of IL-2 and TLR 2 genes involved in the immune system of lactating goats. The study was conducted on 15 lactating Beetal goats for 3 months, which were divided into three groups. Treatment group T1 was kept as a control group with no linseed supplementation, whereas treatment group T2 received ground linseed at the rate 10% of concentrate per day per animal, and treatment group T3 received 20% ground lin...
Source: Animal Biotechnology - October 31, 2023 Category: Biotechnology Authors: Jyoti Shunthwal Zile Singh Sihag Ankit Magotra None Meetu Sajjan Sihag Source Type: research

Gut microbiota absence and transplantation affect diarrhea: an investigation in the germ-free piglet model
Anim Biotechnol. 2023 Oct 31:1-7. doi: 10.1080/10495398.2023.2248200. Online ahead of print.ABSTRACTThis experiment was conducted to explore the effects of gut microbiota on neonatal diarrhea in a germ-free (GF) pig model. Twelve hysterectomy-derived GF piglets were housed in six sterile isolators. Among them, six piglets were treated as the GF group, and the other six piglets were orally introduced with healthy sow fecal suspension and regarded as the fecal microbiota transplantation (FMT) group. Another six piglets from natural birth were considered as the conventional (CV) group. The GF and FMT piglets were hand-fed wit...
Source: Animal Biotechnology - October 31, 2023 Category: Biotechnology Authors: Hua Zhou Bing Yu Jing Sun Hong Chen Zuohua Liu Liangpeng Ge Daiwen Chen Source Type: research

Functional analysis of the < em > GPAT4 < /em > gene mutation predicted to affect splicing
Anim Biotechnol. 2023 Oct 31:1-9. doi: 10.1080/10495398.2023.2269210. Online ahead of print.ABSTRACTThe GPAT4 gene is considered as a potential functional candidate for single nucleotide polymorphism (SNP) studies in dairy cattle breeding due to its association with dairy performance in cattle by encoding an enzyme responsible for the presence of diacylglycerols and triacylglycerols in milk. Using the example of the GPAT4 gene, we applied the minigene splicing assay to analyze the functional consequences of its variant that was predicted to affect normal splicing. The results of functional analysis revealed the sequence va...
Source: Animal Biotechnology - October 31, 2023 Category: Biotechnology Authors: Sergey A Bursakov Anastasia V Kovaleva Artyom V Brigida Oleg G Zaripov Source Type: research

Investigating the impact of linseed supplementation, rich in omega-3 fatty acids, on the expression of immune genes in Beetal Goats
Anim Biotechnol. 2023 Oct 31:1-6. doi: 10.1080/10495398.2023.2263049. Online ahead of print.ABSTRACTThe present study investigated the effect of linseed supplementation on serum profiling and differential expression levels of IL-2 and TLR 2 genes involved in the immune system of lactating goats. The study was conducted on 15 lactating Beetal goats for 3 months, which were divided into three groups. Treatment group T1 was kept as a control group with no linseed supplementation, whereas treatment group T2 received ground linseed at the rate 10% of concentrate per day per animal, and treatment group T3 received 20% ground lin...
Source: Animal Biotechnology - October 31, 2023 Category: Biotechnology Authors: Jyoti Shunthwal Zile Singh Sihag Ankit Magotra None Meetu Sajjan Sihag Source Type: research

Gut microbiota absence and transplantation affect diarrhea: an investigation in the germ-free piglet model
Anim Biotechnol. 2023 Oct 31:1-7. doi: 10.1080/10495398.2023.2248200. Online ahead of print.ABSTRACTThis experiment was conducted to explore the effects of gut microbiota on neonatal diarrhea in a germ-free (GF) pig model. Twelve hysterectomy-derived GF piglets were housed in six sterile isolators. Among them, six piglets were treated as the GF group, and the other six piglets were orally introduced with healthy sow fecal suspension and regarded as the fecal microbiota transplantation (FMT) group. Another six piglets from natural birth were considered as the conventional (CV) group. The GF and FMT piglets were hand-fed wit...
Source: Animal Biotechnology - October 31, 2023 Category: Biotechnology Authors: Hua Zhou Bing Yu Jing Sun Hong Chen Zuohua Liu Liangpeng Ge Daiwen Chen Source Type: research

Functional analysis of the < em > GPAT4 < /em > gene mutation predicted to affect splicing
Anim Biotechnol. 2023 Oct 31:1-9. doi: 10.1080/10495398.2023.2269210. Online ahead of print.ABSTRACTThe GPAT4 gene is considered as a potential functional candidate for single nucleotide polymorphism (SNP) studies in dairy cattle breeding due to its association with dairy performance in cattle by encoding an enzyme responsible for the presence of diacylglycerols and triacylglycerols in milk. Using the example of the GPAT4 gene, we applied the minigene splicing assay to analyze the functional consequences of its variant that was predicted to affect normal splicing. The results of functional analysis revealed the sequence va...
Source: Animal Biotechnology - October 31, 2023 Category: Biotechnology Authors: Sergey A Bursakov Anastasia V Kovaleva Artyom V Brigida Oleg G Zaripov Source Type: research

Investigating the impact of linseed supplementation, rich in omega-3 fatty acids, on the expression of immune genes in Beetal Goats
Anim Biotechnol. 2023 Oct 31:1-6. doi: 10.1080/10495398.2023.2263049. Online ahead of print.ABSTRACTThe present study investigated the effect of linseed supplementation on serum profiling and differential expression levels of IL-2 and TLR 2 genes involved in the immune system of lactating goats. The study was conducted on 15 lactating Beetal goats for 3 months, which were divided into three groups. Treatment group T1 was kept as a control group with no linseed supplementation, whereas treatment group T2 received ground linseed at the rate 10% of concentrate per day per animal, and treatment group T3 received 20% ground lin...
Source: Animal Biotechnology - October 31, 2023 Category: Biotechnology Authors: Jyoti Shunthwal Zile Singh Sihag Ankit Magotra None Meetu Sajjan Sihag Source Type: research

Gut microbiota absence and transplantation affect diarrhea: an investigation in the germ-free piglet model
Anim Biotechnol. 2023 Oct 31:1-7. doi: 10.1080/10495398.2023.2248200. Online ahead of print.ABSTRACTThis experiment was conducted to explore the effects of gut microbiota on neonatal diarrhea in a germ-free (GF) pig model. Twelve hysterectomy-derived GF piglets were housed in six sterile isolators. Among them, six piglets were treated as the GF group, and the other six piglets were orally introduced with healthy sow fecal suspension and regarded as the fecal microbiota transplantation (FMT) group. Another six piglets from natural birth were considered as the conventional (CV) group. The GF and FMT piglets were hand-fed wit...
Source: Animal Biotechnology - October 31, 2023 Category: Biotechnology Authors: Hua Zhou Bing Yu Jing Sun Hong Chen Zuohua Liu Liangpeng Ge Daiwen Chen Source Type: research

Functional analysis of the < em > GPAT4 < /em > gene mutation predicted to affect splicing
Anim Biotechnol. 2023 Oct 31:1-9. doi: 10.1080/10495398.2023.2269210. Online ahead of print.ABSTRACTThe GPAT4 gene is considered as a potential functional candidate for single nucleotide polymorphism (SNP) studies in dairy cattle breeding due to its association with dairy performance in cattle by encoding an enzyme responsible for the presence of diacylglycerols and triacylglycerols in milk. Using the example of the GPAT4 gene, we applied the minigene splicing assay to analyze the functional consequences of its variant that was predicted to affect normal splicing. The results of functional analysis revealed the sequence va...
Source: Animal Biotechnology - October 31, 2023 Category: Biotechnology Authors: Sergey A Bursakov Anastasia V Kovaleva Artyom V Brigida Oleg G Zaripov Source Type: research

Investigating the impact of linseed supplementation, rich in omega-3 fatty acids, on the expression of immune genes in Beetal Goats
Anim Biotechnol. 2023 Oct 31:1-6. doi: 10.1080/10495398.2023.2263049. Online ahead of print.ABSTRACTThe present study investigated the effect of linseed supplementation on serum profiling and differential expression levels of IL-2 and TLR 2 genes involved in the immune system of lactating goats. The study was conducted on 15 lactating Beetal goats for 3 months, which were divided into three groups. Treatment group T1 was kept as a control group with no linseed supplementation, whereas treatment group T2 received ground linseed at the rate 10% of concentrate per day per animal, and treatment group T3 received 20% ground lin...
Source: Animal Biotechnology - October 31, 2023 Category: Biotechnology Authors: Jyoti Shunthwal Zile Singh Sihag Ankit Magotra None Meetu Sajjan Sihag Source Type: research

Gut microbiota absence and transplantation affect diarrhea: an investigation in the germ-free piglet model
Anim Biotechnol. 2023 Oct 31:1-7. doi: 10.1080/10495398.2023.2248200. Online ahead of print.ABSTRACTThis experiment was conducted to explore the effects of gut microbiota on neonatal diarrhea in a germ-free (GF) pig model. Twelve hysterectomy-derived GF piglets were housed in six sterile isolators. Among them, six piglets were treated as the GF group, and the other six piglets were orally introduced with healthy sow fecal suspension and regarded as the fecal microbiota transplantation (FMT) group. Another six piglets from natural birth were considered as the conventional (CV) group. The GF and FMT piglets were hand-fed wit...
Source: Animal Biotechnology - October 31, 2023 Category: Biotechnology Authors: Hua Zhou Bing Yu Jing Sun Hong Chen Zuohua Liu Liangpeng Ge Daiwen Chen Source Type: research

Functional analysis of the < em > GPAT4 < /em > gene mutation predicted to affect splicing
Anim Biotechnol. 2023 Oct 31:1-9. doi: 10.1080/10495398.2023.2269210. Online ahead of print.ABSTRACTThe GPAT4 gene is considered as a potential functional candidate for single nucleotide polymorphism (SNP) studies in dairy cattle breeding due to its association with dairy performance in cattle by encoding an enzyme responsible for the presence of diacylglycerols and triacylglycerols in milk. Using the example of the GPAT4 gene, we applied the minigene splicing assay to analyze the functional consequences of its variant that was predicted to affect normal splicing. The results of functional analysis revealed the sequence va...
Source: Animal Biotechnology - October 31, 2023 Category: Biotechnology Authors: Sergey A Bursakov Anastasia V Kovaleva Artyom V Brigida Oleg G Zaripov Source Type: research