Mutational analysis of FOLR1 and FOLR2 genes in children with Myelomeningocele
This study focused on the mutational analysis of FOLR1 and FOLR2 genes in children suffering from MMC. Myelomeningocele is a rare disorder so twenty blood samples from the children were collected. Primers of selected exons for FOLR1 and FOLR2 genes were designed with the help of PrimerFox software. Extracted DNA was amplified, and PCR based mutational analysis was done to check any type of mutation/SNPs in these genes. Sanger sequencing method was performed to confirm mutation in FOLR1 and FOLR2 genes. The results showed that certain environmental factors (smoking, low socio-economic status of mother bearing MMC fetus) wer...
Source: Acta Biochim Pol - October 26, 2023 Category: Biochemistry Authors: Nageen Hussain Saira Malik Tayyaba Faiz Fiza Shafqat Ayaz Ali Khan Taqweem Ul Haq Waqar Ali Tariq Aziz Metab Alharbi Abdulrahman Alshammari Abdullah F Alasmari Source Type: research
Anesthesia and surgery induce changes in endogenous brain protective protein (RNF146) and delirium-like behavior in aged rats
CONCLUSION: Anesthesia and surgery can lead to POD and neuroinflammation. The expression of RNF146 correlates with delirium and neuroinflammation caused by anesthesia and surgery.PMID:37883680 | DOI:10.18388/abp.2020_6720 (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - October 26, 2023 Category: Biochemistry Authors: Yubo Gao Xu Han Xiuhua Li Shaling Tang Chun Zhang Xiaoxia Yang Majid Alhomrani Abdulhakeem S Alamri Ghulam Nabi Xinli Ni Source Type: research
Mutational analysis of FOLR1 and FOLR2 genes in children with Myelomeningocele
This study focused on the mutational analysis of FOLR1 and FOLR2 genes in children suffering from MMC. Myelomeningocele is a rare disorder so twenty blood samples from the children were collected. Primers of selected exons for FOLR1 and FOLR2 genes were designed with the help of PrimerFox software. Extracted DNA was amplified, and PCR based mutational analysis was done to check any type of mutation/SNPs in these genes. Sanger sequencing method was performed to confirm mutation in FOLR1 and FOLR2 genes. The results showed that certain environmental factors (smoking, low socio-economic status of mother bearing MMC fetus) wer...
Source: Acta Biochim Pol - October 26, 2023 Category: Biochemistry Authors: Nageen Hussain Saira Malik Tayyaba Faiz Fiza Shafqat Ayaz Ali Khan Taqweem Ul Haq Waqar Ali Tariq Aziz Metab Alharbi Abdulrahman Alshammari Abdullah F Alasmari Source Type: research
Anesthesia and surgery induce changes in endogenous brain protective protein (RNF146) and delirium-like behavior in aged rats
CONCLUSION: Anesthesia and surgery can lead to POD and neuroinflammation. The expression of RNF146 correlates with delirium and neuroinflammation caused by anesthesia and surgery.PMID:37883680 | DOI:10.18388/abp.2020_6720 (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - October 26, 2023 Category: Biochemistry Authors: Yubo Gao Xu Han Xiuhua Li Shaling Tang Chun Zhang Xiaoxia Yang Majid Alhomrani Abdulhakeem S Alamri Ghulam Nabi Xinli Ni Source Type: research
Mutational analysis of FOLR1 and FOLR2 genes in children with Myelomeningocele
This study focused on the mutational analysis of FOLR1 and FOLR2 genes in children suffering from MMC. Myelomeningocele is a rare disorder so twenty blood samples from the children were collected. Primers of selected exons for FOLR1 and FOLR2 genes were designed with the help of PrimerFox software. Extracted DNA was amplified, and PCR based mutational analysis was done to check any type of mutation/SNPs in these genes. Sanger sequencing method was performed to confirm mutation in FOLR1 and FOLR2 genes. The results showed that certain environmental factors (smoking, low socio-economic status of mother bearing MMC fetus) wer...
Source: Acta Biochim Pol - October 26, 2023 Category: Biochemistry Authors: Nageen Hussain Saira Malik Tayyaba Faiz Fiza Shafqat Ayaz Ali Khan Taqweem Ul Haq Waqar Ali Tariq Aziz Metab Alharbi Abdulrahman Alshammari Abdullah F Alasmari Source Type: research
Anesthesia and surgery induce changes in endogenous brain protective protein (RNF146) and delirium-like behavior in aged rats
CONCLUSION: Anesthesia and surgery can lead to POD and neuroinflammation. The expression of RNF146 correlates with delirium and neuroinflammation caused by anesthesia and surgery.PMID:37883680 | DOI:10.18388/abp.2020_6720 (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - October 26, 2023 Category: Biochemistry Authors: Yubo Gao Xu Han Xiuhua Li Shaling Tang Chun Zhang Xiaoxia Yang Majid Alhomrani Abdulhakeem S Alamri Ghulam Nabi Xinli Ni Source Type: research
Mutational analysis of FOLR1 and FOLR2 genes in children with Myelomeningocele
This study focused on the mutational analysis of FOLR1 and FOLR2 genes in children suffering from MMC. Myelomeningocele is a rare disorder so twenty blood samples from the children were collected. Primers of selected exons for FOLR1 and FOLR2 genes were designed with the help of PrimerFox software. Extracted DNA was amplified, and PCR based mutational analysis was done to check any type of mutation/SNPs in these genes. Sanger sequencing method was performed to confirm mutation in FOLR1 and FOLR2 genes. The results showed that certain environmental factors (smoking, low socio-economic status of mother bearing MMC fetus) wer...
Source: Acta Biochim Pol - October 26, 2023 Category: Biochemistry Authors: Nageen Hussain Saira Malik Tayyaba Faiz Fiza Shafqat Ayaz Ali Khan Taqweem Ul Haq Waqar Ali Tariq Aziz Metab Alharbi Abdulrahman Alshammari Abdullah F Alasmari Source Type: research
Anesthesia and surgery induce changes in endogenous brain protective protein (RNF146) and delirium-like behavior in aged rats
CONCLUSION: Anesthesia and surgery can lead to POD and neuroinflammation. The expression of RNF146 correlates with delirium and neuroinflammation caused by anesthesia and surgery.PMID:37883680 | DOI:10.18388/abp.2020_6720 (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - October 26, 2023 Category: Biochemistry Authors: Yubo Gao Xu Han Xiuhua Li Shaling Tang Chun Zhang Xiaoxia Yang Majid Alhomrani Abdulhakeem S Alamri Ghulam Nabi Xinli Ni Source Type: research
Mutational analysis of FOLR1 and FOLR2 genes in children with Myelomeningocele
This study focused on the mutational analysis of FOLR1 and FOLR2 genes in children suffering from MMC. Myelomeningocele is a rare disorder so twenty blood samples from the children were collected. Primers of selected exons for FOLR1 and FOLR2 genes were designed with the help of PrimerFox software. Extracted DNA was amplified, and PCR based mutational analysis was done to check any type of mutation/SNPs in these genes. Sanger sequencing method was performed to confirm mutation in FOLR1 and FOLR2 genes. The results showed that certain environmental factors (smoking, low socio-economic status of mother bearing MMC fetus) wer...
Source: Acta Biochim Pol - October 26, 2023 Category: Biochemistry Authors: Nageen Hussain Saira Malik Tayyaba Faiz Fiza Shafqat Ayaz Ali Khan Taqweem Ul Haq Waqar Ali Tariq Aziz Metab Alharbi Abdulrahman Alshammari Abdullah F Alasmari Source Type: research
Anesthesia and surgery induce changes in endogenous brain protective protein (RNF146) and delirium-like behavior in aged rats
CONCLUSION: Anesthesia and surgery can lead to POD and neuroinflammation. The expression of RNF146 correlates with delirium and neuroinflammation caused by anesthesia and surgery.PMID:37883680 | DOI:10.18388/abp.2020_6720 (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - October 26, 2023 Category: Biochemistry Authors: Yubo Gao Xu Han Xiuhua Li Shaling Tang Chun Zhang Xiaoxia Yang Majid Alhomrani Abdulhakeem S Alamri Ghulam Nabi Xinli Ni Source Type: research
Mutational analysis of FOLR1 and FOLR2 genes in children with Myelomeningocele
This study focused on the mutational analysis of FOLR1 and FOLR2 genes in children suffering from MMC. Myelomeningocele is a rare disorder so twenty blood samples from the children were collected. Primers of selected exons for FOLR1 and FOLR2 genes were designed with the help of PrimerFox software. Extracted DNA was amplified, and PCR based mutational analysis was done to check any type of mutation/SNPs in these genes. Sanger sequencing method was performed to confirm mutation in FOLR1 and FOLR2 genes. The results showed that certain environmental factors (smoking, low socio-economic status of mother bearing MMC fetus) wer...
Source: Acta Biochim Pol - October 26, 2023 Category: Biochemistry Authors: Nageen Hussain Saira Malik Tayyaba Faiz Fiza Shafqat Ayaz Ali Khan Taqweem Ul Haq Waqar Ali Tariq Aziz Metab Alharbi Abdulrahman Alshammari Abdullah F Alasmari Source Type: research
The relationship between EMG high frequency and low frequency band amplitude changes correlates with tissue inorganic phosphate levels
In this study, we attempted to derive a non-invasive biomarker for phosphate tissue levels. We analyzed surface electromyography signals taken during 31P spectroscopy of leg muscles in five adult pigs. We induced hypophosphatemia via 20 minutes-long hyperventilation. It turned out that the proportion of the amplitude of the low frequency band and the high frequency band is significantly (p=0.002) correlated with the relative phosphate levels. The electromyographic signal did not correlate significantly with pCO2 levels in the blood, suggesting that the changes in the signal are a result of inorganic phosphate levels, not h...
Source: Acta Biochim Pol - October 18, 2023 Category: Biochemistry Authors: Ma łgorzata Habich Bartosz Pawlinski Kamil Lorenc Maria Sady Katarzyna Siewruk Piotr Zielenkiewicz Zdzislaw Gajewski Jaroslaw Poznanski Leszek Paczek Pawel Szczesny Source Type: research
Circular RNA AGFG1 motivates breast cancer cell proliferation, invasion, migration, and glycolysis by controlling microRNA-653-5p/14-3-3 protein epsilon
Acta Biochim Pol. 2023 Oct 18. doi: 10.18388/abp.2020_6254. Online ahead of print.ABSTRACTA recent Pairwise meta-analysis confirmed that circular RNA AGFG1 (circAGFG1) is abnormally highly expressed in breast cancer (BC) and may be associated with death risk. The purpose of this study was to elucidate the biological role of circAGFG1 in BC and to explore its potential downstream molecular mechanisms. CircAGFG1, miR-653-5p and YWHAE expression in BC tissues and cells were analyzed by RT-qPCR or western blot. Gene expression was regulated by transfection of plasmids or oligonucleotides and the biological behaviors of BC cell...
Source: Acta Biochim Pol - October 18, 2023 Category: Biochemistry Authors: Liang Chen JinXian Qian Ying Shen Xiang Yu Source Type: research
Circular RNA sirtuin-1 restrains the malignant phenotype of non-small cell lung cancer cells via the microRNA-510-5p/SMAD family member 7 axis
Acta Biochim Pol. 2023 Oct 18. doi: 10.18388/abp.2020_6675. Online ahead of print.ABSTRACTCircular RNA (circRNA) sirtuin-1 (SIRT1) is differentially expressed in non-small cell lung cancer (NSCLC), but its specific mechanism is still uncertain. The study was to figure out the latent molecular mechanism of circSIRT1 in NSCLC. The results clarified that circSIRT1 and SMAD family member 7 (SMAD7) were downregulated, but microRNA (miR)-510-5p was upregulated in NSCLC. CircSIRT1 expression was linked with tumor-node-metastasis staging and tumor size in NSCLC patients. Elevating circSIRT1 or suppressing miR-510-5p refrained NSCL...
Source: Acta Biochim Pol - October 18, 2023 Category: Biochemistry Authors: ZiRan Zhao HongYan Zhang Fan Zhang Ying Ji Yue Peng Fei Wang Liang Zhao Source Type: research
The relationship between EMG high frequency and low frequency band amplitude changes correlates with tissue inorganic phosphate levels
In this study, we attempted to derive a non-invasive biomarker for phosphate tissue levels. We analyzed surface electromyography signals taken during 31P spectroscopy of leg muscles in five adult pigs. We induced hypophosphatemia via 20 minutes-long hyperventilation. It turned out that the proportion of the amplitude of the low frequency band and the high frequency band is significantly (p=0.002) correlated with the relative phosphate levels. The electromyographic signal did not correlate significantly with pCO2 levels in the blood, suggesting that the changes in the signal are a result of inorganic phosphate levels, not h...
Source: Acta Biochim Pol - October 18, 2023 Category: Biochemistry Authors: Ma łgorzata Habich Bartosz Pawlinski Kamil Lorenc Maria Sady Katarzyna Siewruk Piotr Zielenkiewicz Zdzislaw Gajewski Jaroslaw Poznanski Leszek Paczek Pawel Szczesny Source Type: research
