The emergence of biological homochirality
Acta Biochim Pol. 2023 Sep 7. doi: 10.18388/abp.2020_6914. Online ahead of print.ABSTRACTThe homochirality of biological molecules is one of the basic mysteries of biogenesis. The predominance of l-amino acids and d-hydrocarbons in living matter stands in contrast to the chemical principle of symmetry between enantiomers. An answer to the puzzle needs to include a plausible explanation of how the natural racemic balance was initially tipped in favor of one enantiomer and how the initial tiny excess was amplified to significant levels. It is also necessary to consider how the imbalance was sustained from returning to a ther...
Source: Acta Biochim Pol - September 7, 2023 Category: Biochemistry Authors: Agnieszka Kiliszek Wojciech Rypniewski Source Type: research
Synergistic effect of Dactolisib/Lys05 combination on autophagy in A549 cells
Acta Biochim Pol. 2023 Sep 7. doi: 10.18388/abp.2020_6694. Online ahead of print.ABSTRACTEffective therapeutic strategies are urgently required to enhance the prognosis of patients suffering from KRAS mutations. Owing to the undruggable nature of KRAS, targeting downstream signaling pathways, namely PI3K/AKT/mTOR, shows antiproliferative and apoptotic effects. Unfortunately, targeting this pathway upregulates autophagy, contributing to reduced drug efficacy. Therefore, it was reasonable to use a combination of kinase inhibitors and autophagy inhibitors to achieve a higher therapeutic benefit. The impact of Dactolisib, a du...
Source: Acta Biochim Pol - September 7, 2023 Category: Biochemistry Authors: Mohammad Abdelwahab Hesham Saeed Nefertiti Elnikhely Hisham Nematalla Source Type: research
irc_PWWP2A promotes lung fibroblast proliferation and fibrosis via the miR-27b-3p/GATA3 axis, thereby aggravating idiopathic pulmonary fibrosis
CONCLUSION: Silencing circ_PWWP2A can inhibit the proliferation of lung fibroblasts induced by TGF-β through the miR-27b-3p/GATA3 axis, and reduce BLM-induced pulmonary fibrosis in rats, which may be a potential therapeutic target for IPF.PMID:37672715 | DOI:10.18388/abp.2020_6459 (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - September 6, 2023 Category: Biochemistry Authors: LiQing Su Ying Nian TianJi Zhu Source Type: research
Formononetin enhances the chemosensitivity of triple negative breast cancer via BTB domain and CNC homolog 1-mediated mitophagy pathways
This study aimed to investigate the effects of formononetin on triple negative breast cancer (TNBC). Clinical samples were collected from patients with TNBC. Overall survival rates were evaluated using the Kaplan-Meier method. Gene expression was determined using immunohistochemistry, immunofluorescence and western blot. Cellular functions were determined using CCK-8, colony formation and propidium iodide (PI) staining. Xenograft assay was performed to further verify the effects of formononetin (FM) on TNBC. We found that FM combined therapy suppressed the metastasis of TNBC and increased the overall survival rates of TNBC...
Source: Acta Biochim Pol - September 6, 2023 Category: Biochemistry Authors: Shan Li Linlian Zhu Yufeng He Ting Sun Source Type: research
Downregulation of circular RNA 00091761 protects against heart failure after myocardial infarction via microRNA-335-3p/ ASCL4 axis
Acta Biochim Pol. 2023 Sep 6. doi: 10.18388/abp.2020_6404. Online ahead of print.ABSTRACTOur research tended to explore the biological roles and expression status of circ_00091761 in HF after MI. The hypoxia reoxygenation (H/R) injured H9c2 cells model was constructed to simulate HF after MI. The expression of circ_0091761 was examined in H/R injured H9c2 cells by qRT-PCR. Then, the effect of circ_0091761 expression on the proliferation of H/R injured H9c2 cells was evaluated by CCK-8 along with TUNEL assay. Secretion of lactate dehydrogenase (LDH), reactive oxygen species (ROS), Fe2+, glutathione (GSH), and malondialdehyd...
Source: Acta Biochim Pol - September 6, 2023 Category: Biochemistry Authors: Qian Wei Mengni Jiang Bin Tang Lanlan You Lin Zhao Source Type: research
The Upregulation of miR-22 alleviates oxygen-glucose deprivation/reperfusion-induced injury by targeting Tiam1 in SH-SY5Y cells
In this study, we evaluated whether miR-22 participates in I/R-induced neuronal injury and the potential mechanism by using an oxygen-glucose deprivation/reperfusion (OGD/R) model in vitro. Our results showed that miR-22 was significantly down-regulated in SH-SY5Y cells suffering from OGD/R. Up-regulation of miR-22 by its specific mimic could protect SH-SY5Y cells against OGD/R-induced injury. The luciferase reporter assay demonstrated that T-cell lymphoma invasion and metastasis 1 (Tiam1) was a direct target of miR-22. MiR-22 mimic obviously inhibited Tiam1 expression in OGD/R-exposed SH-SY5Y cells. Tiam1 siRNA could atte...
Source: Acta Biochim Pol - September 6, 2023 Category: Biochemistry Authors: Jiansong Yin Yu Wan Jing Wang Mei Xue Source Type: research
Microbiome Relationship of gut microbiota and immunological response in obesity-related non-alcoholic fatty liver disease in children
Acta Biochim Pol. 2023 Sep 6. doi: 10.18388/abp.2020_6573. Online ahead of print.ABSTRACTIn the development of NAFLD plays an important role the intestinal microflora. Our aim was to characterize role microbiota in children. Distinctive gut microbiota composition was observed in children, characterized and short-chain fatty acid producing bacteria. For the treatment of NAFLD it is possible by therapeutic manipulations with prebiotics and probiotics to modulate the gut microbiota and maintain the integrity of the intestinal barrier are potential agents.PMID:37672729 | DOI:10.18388/abp.2020_6573 (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - September 6, 2023 Category: Biochemistry Authors: Aldona Wierzbicka-Ruci ńska Source Type: research
Bionics investigation of blood 25-hydroxyvitamin D in the interpretable biomechanics diagnosis of childhood anemia
Acta Biochim Pol. 2023 Sep 6. doi: 10.18388/abp.2020_6676. Online ahead of print.ABSTRACTVitamin D deficiency (VDD) causes a wide range of health problems, including anemia in infants. If not treated promptly, it may create serious issues for infants with long-term impacts. Therefore, a satisfactory solution to this problem is required. This investigation was to explore the correlation between the blood 25-hydroxyvitamin D (25(OH)D) levels and childhood anemia. In this investigation, a cross-sectional examination was performed on 2,942 babies ranging in age from 2 to 36 months and classified into three cohorts: VDD (Vitami...
Source: Acta Biochim Pol - September 6, 2023 Category: Biochemistry Authors: Lechi Zhang Aijie Huang Jingye Cai Jiangting Hou Hongyan Deng Chenxiao Liu Source Type: research
CRISPR-Cas9 guided rna based model for the treatment of Amyotrophic Lateral Sclerosis: A progressive neurodegenerative disorder
This study highlights the potential of CRISPR-Cas9 as a promising solution for the development of new treatments for ALS. Further research is required to validate these findings in preclinical and clinical trials and to establish the safety and efficacy of this approach in the treatment of ALS.PMID:37672770 | DOI:10.18388/abp.2020_6789 (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - September 6, 2023 Category: Biochemistry Authors: Muhammad Naveed Muhammad Aqib Shabbir Tariq Aziz Hafiz Muhammad Hurraira Sayyeda Fatima Zaidi Ramsha Athar Hassan Anwer Chattha Metab Alharbi Abdulrahman Alshammari Abdullah F Alasmari Source Type: research
Investigation of VEGF (rs 699947) polymorphism in the progression of Rheumatoid Arthritis (RA) and in-silico nanoparticle drug delivery of potential phytochemicals to cure RA
In this study, it had been shown that allele A frequency was significantly higher than that of allele C in RA-positive patients as compared to controls. Hence it depicts that allele A of VEGF (rs699947) can increase the risk of RA while allele C has a protective role. The phytochemicals which showed maximum binding affinity at the inhibitory site of BTK include beta boswellic acid, tanshinone, and baicalin. These phytochemicals as BTK inhibitor give insights to use them as anti-arthritic compounds by nanoparticle drug delivery mechanism.PMID:37669474 | DOI:10.18388/abp.2020_6654 (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - September 5, 2023 Category: Biochemistry Authors: Nageen Hussain Mohsin Mumtaz Mohammad Adil Abad Ali Nadeem Abid Sarwar Tariq Aziz Metab Alharbi Abdulrahman Alshammari Abdullah F Alasmari Mousa Essa Alharbi Source Type: research
Investigation of VEGF (rs 699947) polymorphism in the progression of Rheumatoid Arthritis (RA) and in-silico nanoparticle drug delivery of potential phytochemicals to cure RA
In this study, it had been shown that allele A frequency was significantly higher than that of allele C in RA-positive patients as compared to controls. Hence it depicts that allele A of VEGF (rs699947) can increase the risk of RA while allele C has a protective role. The phytochemicals which showed maximum binding affinity at the inhibitory site of BTK include beta boswellic acid, tanshinone, and baicalin. These phytochemicals as BTK inhibitor give insights to use them as anti-arthritic compounds by nanoparticle drug delivery mechanism.PMID:37669474 | DOI:10.18388/abp.2020_6654 (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - September 5, 2023 Category: Biochemistry Authors: Nageen Hussain Mohsin Mumtaz Mohammad Adil Abad Ali Nadeem Abid Sarwar Tariq Aziz Metab Alharbi Abdulrahman Alshammari Abdullah F Alasmari Mousa Essa Alharbi Source Type: research
Discovery of a novel genetic variant in the N-acetyltransferase2 (NAT2) gene that is associated with bladder cancer risk
This study aimed to discover novel SNPs in NAT2 and to assess the association with BC. This was a case-control study among 120 BC patients and 120 controls. Amplification of a 446 bp fragment of NAT2 encoding the N-catalytic domain was conducted using a polymerase chain reaction. Gene sequencing was done using Sanger-based technology. A total of 40 SNPs were detected. Two variants were significantly associated with BC (p<0.05); namely a novel c.87G>A and the reported c.341T>C. Regarding c.87G>A, genotype distribution was significantly associated with BC and subgroup analysis confirmed that this was significant ...
Source: Acta Biochim Pol - August 18, 2023 Category: Biochemistry Authors: Lina Elsalem Ahmad Al Shatnawi Mahmoud A Alfaqih Ayat Alshoh Saddam Al Demour Ali Al-Daghmin Omar Halalsheh Khalid Kheirallah Mamoun Ahram Source Type: research
MiR-96-5p is involved in permethrin-promoted proliferation and migration of breast cancer cells
In conclusion, these results suggest that miR-96-5p is involved in permethrin-promoted proliferation and migration of breast cancer cells by targeting HOXA5.PMID:37595068 | DOI:10.18388/abp.2020_6533 (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - August 18, 2023 Category: Biochemistry Authors: Yi Yan Tian Wen Long Xi Niu Jia Fu Wang Sheng Li Source Type: research
Circ-POLA2-mediated miR-138-5p/SEMA4C axis affects colon cancer cell activities
This study aimed to investigate the mechanism of circ-POLA2 in colon cancer (CC). Circ-POLA2, miR-138-5p, and SEMA4C levels in CC tissues and cells were recorded. The influences mediated by circ-POLA2, miR-138-5p or SEMA4C on cell proliferation, migration, invasion, and apoptosis were determined. The feedback loop of circ-POLA2/miR-138-5p/SEMA4C was surveyed. As measured, circ-POLA2 and SEMA4C were highly expressed, while miR-138-5p was poorly expressed. Meanwhile, circ-POLA2 could mediate SEMA4C through miR-138-5p targeting. Circ-POLA2 knockdown caused the blockade for cell activities, but this effect was alleviated by mi...
Source: Acta Biochim Pol - August 18, 2023 Category: Biochemistry Authors: YanDong Huang QingYang Bai HongBo Yu YanRu Li Hao Lu HuiMin Kang XueWei Shi Kai Feng Source Type: research
Characterization and gastroprotective effects of Rosa brunonii Lindl. fruit on gastric mucosal injury in experimental rats - A preliminary study
This study demonstrated that oral administration of RBFCE possesses a significant gastroprotective effect due to its anti-secretory and cytoprotective mechanisms. Our findings support the traditional use of RBF to treat the gastric ulcer.PMID:37595092 | DOI:10.18388/abp.2020_6772 (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - August 18, 2023 Category: Biochemistry Authors: Ejaz Ahmad Muhammad Jahangeer Zahid Mahmood Akhtar Tariq Aziz Metab Alharbi Abdulrahman Alshammari Abdullah F Alasmari Nadeem Irfan Bukhari Source Type: research
