Mutational analysis of FOLR1 and FOLR2 genes in children with Myelomeningocele
This study focused on the mutational analysis of FOLR1 and FOLR2 genes in children suffering from MMC. Myelomeningocele is a rare disorder so twenty blood samples from the children were collected. Primers of selected exons for FOLR1 and FOLR2 genes were designed with the help of PrimerFox software. Extracted DNA was amplified, and PCR based mutational analysis was done to check any type of mutation/SNPs in these genes. Sanger sequencing method was performed to confirm mutation in FOLR1 and FOLR2 genes. The results showed that certain environmental factors (smoking, low socio-economic status of mother bearing MMC fetus) were found to be significantly (P<0.05) associated with MMC but no mutation in the selected exons of FOLR1 and FOLR2 genes was detected. Thus, genetic variations in the folate transporter gene may have no role in the progression of MMC in the studied population.PMID:37883728 | DOI:10.18388/abp.2020_6729
Source: Acta Biochim Pol - Category: Biochemistry Authors: Nageen Hussain Saira Malik Tayyaba Faiz Fiza Shafqat Ayaz Ali Khan Taqweem Ul Haq Waqar Ali Tariq Aziz Metab Alharbi Abdulrahman Alshammari Abdullah F Alasmari Source Type: research