Criteria analysis for kinetics curves of initiated blood serum chemiluminescence
Acta Biochim Pol. 2023 Sep 7. doi: 10.18388/abp.2020_6807. Online ahead of print.ABSTRACTThe chemiluminescence (CL) methods unlike the other methods of determining free radicals (FR) allow investigating the kinetics of the derivation and recombination of radicals/antioxidants, and thus the development and attenuation of the process/processes after excitation in time. However, these methods are of limited application because the knowledge of the explored parameters is insufficient (maximum intensity and integrated area under the kinetics plot). The kinetics is studied by the CL methods and a new parameter (IR-criterion) of ...
Source: Acta Biochim Pol - September 7, 2023 Category: Biochemistry Authors: Iryna Oliynyk Source Type: research

The emergence of biological homochirality
Acta Biochim Pol. 2023 Sep 7. doi: 10.18388/abp.2020_6914. Online ahead of print.ABSTRACTThe homochirality of biological molecules is one of the basic mysteries of biogenesis. The predominance of l-amino acids and d-hydrocarbons in living matter stands in contrast to the chemical principle of symmetry between enantiomers. An answer to the puzzle needs to include a plausible explanation of how the natural racemic balance was initially tipped in favor of one enantiomer and how the initial tiny excess was amplified to significant levels. It is also necessary to consider how the imbalance was sustained from returning to a ther...
Source: Acta Biochim Pol - September 7, 2023 Category: Biochemistry Authors: Agnieszka Kiliszek Wojciech Rypniewski Source Type: research

Synergistic effect of Dactolisib/Lys05 combination on autophagy in A549 cells
Acta Biochim Pol. 2023 Sep 7. doi: 10.18388/abp.2020_6694. Online ahead of print.ABSTRACTEffective therapeutic strategies are urgently required to enhance the prognosis of patients suffering from KRAS mutations. Owing to the undruggable nature of KRAS, targeting downstream signaling pathways, namely PI3K/AKT/mTOR, shows antiproliferative and apoptotic effects. Unfortunately, targeting this pathway upregulates autophagy, contributing to reduced drug efficacy. Therefore, it was reasonable to use a combination of kinase inhibitors and autophagy inhibitors to achieve a higher therapeutic benefit. The impact of Dactolisib, a du...
Source: Acta Biochim Pol - September 7, 2023 Category: Biochemistry Authors: Mohammad Abdelwahab Hesham Saeed Nefertiti Elnikhely Hisham Nematalla Source Type: research

In silico explorations of bacterial mercuric reductase as an ecofriendly bioremediator for noxious mercuric intoxications
In this study, a mercuric reductase was extracted from Pseudomonas aeruginosa. The sequence of the enzyme was retrieved from the literature and structural homologs were identified. The protein bonded with Mercuric compounds and their interaction was briefly studied. Autodock Vina was used to perform a molecular docking with the target protein. Results showed that the sequence consists of most of the random coil 44.74% followed by α-helix and B-turns. Moreover, the protein was predicted to have a FAD/NAD(P)-binding domain. The virulence factor prediction using different approaches of Virulentpred and VICMpred suggested tha...
Source: Acta Biochim Pol - September 7, 2023 Category: Biochemistry Authors: Muhammad Naveed Muhammad Aqib Shabbir Tariq Aziz Ayesha Saleem Rida Naveed Ayaz Ali Khan Taqweem Ul Haq Metab Alharbi Abdulrahman Alshammari Abdullah F Alasmari Source Type: research

Design and development of new inhibitors against breast cancer, Monkeypox and Marburg virus by modification of natural Fisetin via in silico and SAR studies
Acta Biochim Pol. 2023 Sep 7. doi: 10.18388/abp.2020_6667. Online ahead of print.ABSTRACTThe natural Fisetin and its derivatives have been shown to have effective bioactivity and strong pharmacological profile, which is continuously drawing the interest of therapeutic applications to the development of new biomolecules against Breast cancer and Monkeypox, and Marburg viral infection, while computational approaches and the study of their structure-activity relationship (SAR) are the most eloquent and reliable platform for performing their hypothetical profile renovation. So, the main perspective of this investigation is to ...
Source: Acta Biochim Pol - September 7, 2023 Category: Biochemistry Authors: Shopnil Akash Md Mominur Rahman Clara Mariana Gon çalves Lima Talha Bin Emran Sharifa Sultana Sumaira Naz Tariq Aziz Metab Alharbi Abdulrahman Alshammari Abdullah F Alasmari Source Type: research

Criteria analysis for kinetics curves of initiated blood serum chemiluminescence
Acta Biochim Pol. 2023 Sep 7. doi: 10.18388/abp.2020_6807. Online ahead of print.ABSTRACTThe chemiluminescence (CL) methods unlike the other methods of determining free radicals (FR) allow investigating the kinetics of the derivation and recombination of radicals/antioxidants, and thus the development and attenuation of the process/processes after excitation in time. However, these methods are of limited application because the knowledge of the explored parameters is insufficient (maximum intensity and integrated area under the kinetics plot). The kinetics is studied by the CL methods and a new parameter (IR-criterion) of ...
Source: Acta Biochim Pol - September 7, 2023 Category: Biochemistry Authors: Iryna Oliynyk Source Type: research

The emergence of biological homochirality
Acta Biochim Pol. 2023 Sep 7. doi: 10.18388/abp.2020_6914. Online ahead of print.ABSTRACTThe homochirality of biological molecules is one of the basic mysteries of biogenesis. The predominance of l-amino acids and d-hydrocarbons in living matter stands in contrast to the chemical principle of symmetry between enantiomers. An answer to the puzzle needs to include a plausible explanation of how the natural racemic balance was initially tipped in favor of one enantiomer and how the initial tiny excess was amplified to significant levels. It is also necessary to consider how the imbalance was sustained from returning to a ther...
Source: Acta Biochim Pol - September 7, 2023 Category: Biochemistry Authors: Agnieszka Kiliszek Wojciech Rypniewski Source Type: research

Synergistic effect of Dactolisib/Lys05 combination on autophagy in A549 cells
Acta Biochim Pol. 2023 Sep 7. doi: 10.18388/abp.2020_6694. Online ahead of print.ABSTRACTEffective therapeutic strategies are urgently required to enhance the prognosis of patients suffering from KRAS mutations. Owing to the undruggable nature of KRAS, targeting downstream signaling pathways, namely PI3K/AKT/mTOR, shows antiproliferative and apoptotic effects. Unfortunately, targeting this pathway upregulates autophagy, contributing to reduced drug efficacy. Therefore, it was reasonable to use a combination of kinase inhibitors and autophagy inhibitors to achieve a higher therapeutic benefit. The impact of Dactolisib, a du...
Source: Acta Biochim Pol - September 7, 2023 Category: Biochemistry Authors: Mohammad Abdelwahab Hesham Saeed Nefertiti Elnikhely Hisham Nematalla Source Type: research

In silico explorations of bacterial mercuric reductase as an ecofriendly bioremediator for noxious mercuric intoxications
In this study, a mercuric reductase was extracted from Pseudomonas aeruginosa. The sequence of the enzyme was retrieved from the literature and structural homologs were identified. The protein bonded with Mercuric compounds and their interaction was briefly studied. Autodock Vina was used to perform a molecular docking with the target protein. Results showed that the sequence consists of most of the random coil 44.74% followed by α-helix and B-turns. Moreover, the protein was predicted to have a FAD/NAD(P)-binding domain. The virulence factor prediction using different approaches of Virulentpred and VICMpred suggested tha...
Source: Acta Biochim Pol - September 7, 2023 Category: Biochemistry Authors: Muhammad Naveed Muhammad Aqib Shabbir Tariq Aziz Ayesha Saleem Rida Naveed Ayaz Ali Khan Taqweem Ul Haq Metab Alharbi Abdulrahman Alshammari Abdullah F Alasmari Source Type: research

Design and development of new inhibitors against breast cancer, Monkeypox and Marburg virus by modification of natural Fisetin via in silico and SAR studies
Acta Biochim Pol. 2023 Sep 7. doi: 10.18388/abp.2020_6667. Online ahead of print.ABSTRACTThe natural Fisetin and its derivatives have been shown to have effective bioactivity and strong pharmacological profile, which is continuously drawing the interest of therapeutic applications to the development of new biomolecules against Breast cancer and Monkeypox, and Marburg viral infection, while computational approaches and the study of their structure-activity relationship (SAR) are the most eloquent and reliable platform for performing their hypothetical profile renovation. So, the main perspective of this investigation is to ...
Source: Acta Biochim Pol - September 7, 2023 Category: Biochemistry Authors: Shopnil Akash Md Mominur Rahman Clara Mariana Gon çalves Lima Talha Bin Emran Sharifa Sultana Sumaira Naz Tariq Aziz Metab Alharbi Abdulrahman Alshammari Abdullah F Alasmari Source Type: research

Criteria analysis for kinetics curves of initiated blood serum chemiluminescence
Acta Biochim Pol. 2023 Sep 7. doi: 10.18388/abp.2020_6807. Online ahead of print.ABSTRACTThe chemiluminescence (CL) methods unlike the other methods of determining free radicals (FR) allow investigating the kinetics of the derivation and recombination of radicals/antioxidants, and thus the development and attenuation of the process/processes after excitation in time. However, these methods are of limited application because the knowledge of the explored parameters is insufficient (maximum intensity and integrated area under the kinetics plot). The kinetics is studied by the CL methods and a new parameter (IR-criterion) of ...
Source: Acta Biochim Pol - September 7, 2023 Category: Biochemistry Authors: Iryna Oliynyk Source Type: research

The emergence of biological homochirality
Acta Biochim Pol. 2023 Sep 7. doi: 10.18388/abp.2020_6914. Online ahead of print.ABSTRACTThe homochirality of biological molecules is one of the basic mysteries of biogenesis. The predominance of l-amino acids and d-hydrocarbons in living matter stands in contrast to the chemical principle of symmetry between enantiomers. An answer to the puzzle needs to include a plausible explanation of how the natural racemic balance was initially tipped in favor of one enantiomer and how the initial tiny excess was amplified to significant levels. It is also necessary to consider how the imbalance was sustained from returning to a ther...
Source: Acta Biochim Pol - September 7, 2023 Category: Biochemistry Authors: Agnieszka Kiliszek Wojciech Rypniewski Source Type: research

Synergistic effect of Dactolisib/Lys05 combination on autophagy in A549 cells
Acta Biochim Pol. 2023 Sep 7. doi: 10.18388/abp.2020_6694. Online ahead of print.ABSTRACTEffective therapeutic strategies are urgently required to enhance the prognosis of patients suffering from KRAS mutations. Owing to the undruggable nature of KRAS, targeting downstream signaling pathways, namely PI3K/AKT/mTOR, shows antiproliferative and apoptotic effects. Unfortunately, targeting this pathway upregulates autophagy, contributing to reduced drug efficacy. Therefore, it was reasonable to use a combination of kinase inhibitors and autophagy inhibitors to achieve a higher therapeutic benefit. The impact of Dactolisib, a du...
Source: Acta Biochim Pol - September 7, 2023 Category: Biochemistry Authors: Mohammad Abdelwahab Hesham Saeed Nefertiti Elnikhely Hisham Nematalla Source Type: research

In silico explorations of bacterial mercuric reductase as an ecofriendly bioremediator for noxious mercuric intoxications
In this study, a mercuric reductase was extracted from Pseudomonas aeruginosa. The sequence of the enzyme was retrieved from the literature and structural homologs were identified. The protein bonded with Mercuric compounds and their interaction was briefly studied. Autodock Vina was used to perform a molecular docking with the target protein. Results showed that the sequence consists of most of the random coil 44.74% followed by α-helix and B-turns. Moreover, the protein was predicted to have a FAD/NAD(P)-binding domain. The virulence factor prediction using different approaches of Virulentpred and VICMpred suggested tha...
Source: Acta Biochim Pol - September 7, 2023 Category: Biochemistry Authors: Muhammad Naveed Muhammad Aqib Shabbir Tariq Aziz Ayesha Saleem Rida Naveed Ayaz Ali Khan Taqweem Ul Haq Metab Alharbi Abdulrahman Alshammari Abdullah F Alasmari Source Type: research

Design and development of new inhibitors against breast cancer, Monkeypox and Marburg virus by modification of natural Fisetin via in silico and SAR studies
Acta Biochim Pol. 2023 Sep 7. doi: 10.18388/abp.2020_6667. Online ahead of print.ABSTRACTThe natural Fisetin and its derivatives have been shown to have effective bioactivity and strong pharmacological profile, which is continuously drawing the interest of therapeutic applications to the development of new biomolecules against Breast cancer and Monkeypox, and Marburg viral infection, while computational approaches and the study of their structure-activity relationship (SAR) are the most eloquent and reliable platform for performing their hypothetical profile renovation. So, the main perspective of this investigation is to ...
Source: Acta Biochim Pol - September 7, 2023 Category: Biochemistry Authors: Shopnil Akash Md Mominur Rahman Clara Mariana Gon çalves Lima Talha Bin Emran Sharifa Sultana Sumaira Naz Tariq Aziz Metab Alharbi Abdulrahman Alshammari Abdullah F Alasmari Source Type: research