Circ-PGPEP1 augments renal cell carcinoma proliferation, Warburg effect, and distant metastasis
This study probed the molecular mechanism of circPGPEP1 regulating RCC proliferation, Warburg effect, and distant metastasis by targeting the miR-378a-3p/JPT1 axis. Here identified higher circPGPEP1 expression in RCC tissues and cells by RT-qPCR, and high levels of circPGPEP1 were positively correlated with high histological grade and distant metastasis in RCC patients. Furthermore, patients with high levels of circPGPEP1 had a worse survival prognosis. Functional assays presented that knockdown of circPGPEP1 inhibited RCC proliferation, invasion, migration, EMT, and Warburg effect. Dual-luciferase reporter assay, RNA immu...
Source: Acta Biochim Pol - September 18, 2023 Category: Biochemistry Authors: PeiRui Wang Jin Chen Xin Ye RuYi Wang Lin Chen HanChao Zhang Source Type: research
NADPH oxidase 4 facilitates progression of chondrosarcoma via generation of reactive oxygen species
Acta Biochim Pol. 2023 Sep 18;70(3):685-692. doi: 10.18388/abp.2020_6580.ABSTRACTNicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4) is an enzyme that regulates reactive oxygen species (ROS) generation, and its function in the development of chondrosarcoma remains unclear. In the present study, we studied NOX4 expression in chondrosarcoma by immunochemical examination, and analyzed the role of NOX4 in viability and apoptosis of human chondrosarcoma cell line SW1353 using NOX4 siRNA or NOX4 inhibitor GKT137831. NOX4 level significantly increased in tumor compared to that in para-carcinoma sample. The levels of NOX4...
Source: Acta Biochim Pol - September 18, 2023 Category: Biochemistry Authors: Zheng Jun Wang Lei Fang Ce Ren Wen Tao Meng Xiang Hui Qing Ci Nan Source Type: research
Silencing lncRNA EZR ‑AS1 induces apoptosis and attenuates the malignant properties of lung adenocarcinoma cells
This study aimed to investigate the effect of silencing long non-coding RNA (lncRNA) EZR‑AS1 on the biological behaviors of lung adenocarcinoma (ADC) cells. EZR‑AS1 expression levels in lung ADC tissues and cells, as well as in adjacent non-cancerous tissues, were determined using quantitative reverse transcription polymerase chain reaction (qRT-PCR). EZR‑AS1 was knocked down in two lung ADC cell lines using small interfering RNA specific for EZR‑AS1 (siEZR‑AS1). Proliferation, migration, and apoptosis of EZR‑AS1-knockdown cells were assessed using the CCK-8 viability assay, flow cytometry, or wound healing exp...
Source: Acta Biochim Pol - September 18, 2023 Category: Biochemistry Authors: Xianjing Yu Lixue Wu Zhongcui Lu Junli Zhang Yunfeng Zhou Source Type: research
circROCK1 Promotes septic myocardial injury through regulating miR-96-5p/OXSR1 axis
CONCLUSION: CircROCK1 promotes myocardial injury in septic mice by regulating the miR-96-5p/OXSR1 axis, and it can be used as a potential target for treating septic myocardial dysfunction.PMID:37721476 | DOI:10.18388/abp.2020_6547 (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - September 18, 2023 Category: Biochemistry Authors: ZhiYu He Lingling Xu Xiaojun Zeng Biqing Yang Peiying Liu Dunzheng Han Hao Xue Bihui Luo Source Type: research
Circular RNA CSPP1 motivates renal cell carcinoma carcinogenesis and the Warburg effect by targeting RAC1 through microRNA-493-5p
Acta Biochim Pol. 2023 Sep 18. doi: 10.18388/abp.2020_6299. Online ahead of print.ABSTRACTCircular RNAs (circRNAs) take on regulatory roles in renal cell carcinoma (RCC). The research's goal was to figure out circ-CSPP1's role and molecular mechanism in RCC. The results clarified that circ-CSPP1 expression was enhanced in RCC. Down-regulating circ-CSPP1 refrained the proliferation, migration, invasion, and Warburg effect (aerobic glycolysis), but accelerated apoptosis of RCC cells. The luciferase activity assay exhibited that circ-CSPP1 could perform as an endogenous sponge for miR-493-5p. Elevating miR-493-5p repressed RC...
Source: Acta Biochim Pol - September 18, 2023 Category: Biochemistry Authors: Dong Zhang XiaoJie Yang QiDong Luo DeLia Fu HongLiang Li Peng Zhang Chong Tie Source Type: research
Circ-PGPEP1 augments renal cell carcinoma proliferation, Warburg effect, and distant metastasis
This study probed the molecular mechanism of circPGPEP1 regulating RCC proliferation, Warburg effect, and distant metastasis by targeting the miR-378a-3p/JPT1 axis. Here identified higher circPGPEP1 expression in RCC tissues and cells by RT-qPCR, and high levels of circPGPEP1 were positively correlated with high histological grade and distant metastasis in RCC patients. Furthermore, patients with high levels of circPGPEP1 had a worse survival prognosis. Functional assays presented that knockdown of circPGPEP1 inhibited RCC proliferation, invasion, migration, EMT, and Warburg effect. Dual-luciferase reporter assay, RNA immu...
Source: Acta Biochim Pol - September 18, 2023 Category: Biochemistry Authors: PeiRui Wang Jin Chen Xin Ye RuYi Wang Lin Chen HanChao Zhang Source Type: research
NADPH oxidase 4 facilitates progression of chondrosarcoma via generation of reactive oxygen species
Acta Biochim Pol. 2023 Sep 18. doi: 10.18388/abp.2020_6580. Online ahead of print.ABSTRACTNicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4) is an enzyme that regulates reactive oxygen species (ROS) generation, and its function in the development of chondrosarcoma remains unclear. In the present study, we studied NOX4 expression in chondrosarcoma by immunochemical examination, and analyzed the role of NOX4 in viability and apoptosis of human chondrosarcoma cell line SW1353 using NOX4 siRNA or NOX4 inhibitor GKT137831. NOX4 level significantly increased in tumor compared to that in para-carcinoma sample. The level...
Source: Acta Biochim Pol - September 18, 2023 Category: Biochemistry Authors: Zheng Jun Wang Lei Fang Ce Ren Wen Tao Meng Xiang Hui Qing Ci Nan Source Type: research
Silencing lncRNA EZR ‑AS1 induces apoptosis and attenuates the malignant properties of lung adenocarcinoma cells
This study aimed to investigate the effect of silencing long non-coding RNA (lncRNA) EZR‑AS1 on the biological behaviors of lung adenocarcinoma (ADC) cells. EZR‑AS1 expression levels in lung ADC tissues and cells, as well as in adjacent non-cancerous tissues, were determined using quantitative reverse transcription polymerase chain reaction (qRT-PCR). EZR‑AS1 was knocked down in two lung ADC cell lines using small interfering RNA specific for EZR‑AS1 (siEZR‑AS1). Proliferation, migration, and apoptosis of EZR‑AS1-knockdown cells were assessed using the CCK-8 viability assay, flow cytometry, or wound healing exp...
Source: Acta Biochim Pol - September 18, 2023 Category: Biochemistry Authors: Xianjing Yu Lixue Wu Zhongcui Lu Junli Zhang Yunfeng Zhou Source Type: research
An mRNA-based reverse-vaccinology strategy to stimulate the immune response against Nipah virus in humans using fusion glycoproteins
Acta Biochim Pol. 2023 Sep 17. doi: 10.18388/abp.2020_6721. Online ahead of print.ABSTRACTThe zoonotic pathogen, Nipah virus, is considered a potential healthcare threat due to its high mortality rates and detrimental symptoms like encephalitis. Ribavirin, an antiviral drug helps in overcoming the number of casualties and reducing the mortality rate, but no long-lasting solution has been proposed yet putting global health security in jeopardy. Given the cognizance of mRNA-based vaccines as safe and efficacious preventative strategies against pathogens, the current study has utilized the reverse-vaccinology approach coupled...
Source: Acta Biochim Pol - September 17, 2023 Category: Biochemistry Authors: Muhammad Naveed Sarmad Mehmood Tariq Aziz Muhammad Hammad Arif Urooj Ali Faisal Nouroz Christos Zacharis Metab Alharbi Abdulrahman Alshammari Abdullah F Alasmari Source Type: research
Recent studies on non-invasive biomarkers useful in biliary atresia - a literature review
Acta Biochim Pol. 2023 Sep 17. doi: 10.18388/abp.2020_6858. Online ahead of print.ABSTRACTThe aim of this review is to specify new potential reliable and non-invasive methods for the diagnosis of biliary atresia (BA) that could shorten the way to diagnose BA, and finally the surgical treatment. Apart from the biomarkers that have been proven helpful and are used nowadays in neonatal wards, there are several new potential biomarkers that researchers have found to be helpful in the diagnosis of biliary atresia. Circulating microRNAs, matrix metalloproteinase-7, stool proteins, interleukin-33, Th17-associated cytokines, urina...
Source: Acta Biochim Pol - September 17, 2023 Category: Biochemistry Authors: Anna Lew-Tusk Marta P ęksa Teresa Stachowicz-Stencel Source Type: research
Osteosarcoma's genetic landscape painted by genes' mutations
CONCLUSIONS: Their role in cancerogenesis was confirmed by the analysis of available articles published previously. The results of the study indicate that examination of selected genes' mutations might help to identify patients' predisposition to OS development, as well as monitor the disease progression, and establish prognosis. However, to fully understand the pathogenesis of OS further studies are required.PMID:37717265 | DOI:10.18388/abp.2020_6869 (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - September 17, 2023 Category: Biochemistry Authors: Wiktoria Urban Dagmara Krzysta ńska Micha ł Piekarz Jerzy Nazar Anna Jankowska Source Type: research
Impact of diet modification on body mass and kidney function in patients with diabetic nephropathy: a pilot study
CONCLUSIONS: The study shows that obesity is a common comorbid disease in patients with DN and that dietary intervention is associated with a significant reduction in body mass and stabilization of eGFR in these patients.PMID:37717272 | DOI:10.18388/abp.2020_6889 (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - September 17, 2023 Category: Biochemistry Authors: Ma łgorzata Kaczkan Sylwia Czaja-Stolc Ma łgorzata Sikorska-Wiśniewska Micha ł Chmielewski Alicja D ębska-Ślizień Sylwia Ma łgorzewicz Source Type: research
Pheochromocytoma and paraganglioma in children and adolescents
Acta Biochim Pol. 2023 Sep 17. doi: 10.18388/abp.2020_6955. Online ahead of print.ABSTRACTPheochromocytoma (PPC) and paraganglioma (PGL) are the tumors that rarely occur in the pediatric population (PPGL). Both originate from chromaffin cells, pheochromocytoma is localized in the adrenal gland, whereas paragangliomas are regarded as the tumors present in other localizations, from head to the pelvis. The clinical image is characterized by the presence of the sustained hypertension, headaches, sweating, palpitations. The symptoms are caused by the catecholamine secretion or are related to tumor mass pressure on different org...
Source: Acta Biochim Pol - September 17, 2023 Category: Biochemistry Authors: Teresa Stachowicz-Stencel Natalia Pasikowska Anna Synakiewicz Source Type: research
An mRNA-based reverse-vaccinology strategy to stimulate the immune response against Nipah virus in humans using fusion glycoproteins
Acta Biochim Pol. 2023 Sep 17. doi: 10.18388/abp.2020_6721. Online ahead of print.ABSTRACTThe zoonotic pathogen, Nipah virus, is considered a potential healthcare threat due to its high mortality rates and detrimental symptoms like encephalitis. Ribavirin, an antiviral drug helps in overcoming the number of casualties and reducing the mortality rate, but no long-lasting solution has been proposed yet putting global health security in jeopardy. Given the cognizance of mRNA-based vaccines as safe and efficacious preventative strategies against pathogens, the current study has utilized the reverse-vaccinology approach coupled...
Source: Acta Biochim Pol - September 17, 2023 Category: Biochemistry Authors: Muhammad Naveed Sarmad Mehmood Tariq Aziz Muhammad Hammad Arif Urooj Ali Faisal Nouroz Christos Zacharis Metab Alharbi Abdulrahman Alshammari Abdullah F Alasmari Source Type: research
Recent studies on non-invasive biomarkers useful in biliary atresia - a literature review
Acta Biochim Pol. 2023 Sep 17. doi: 10.18388/abp.2020_6858. Online ahead of print.ABSTRACTThe aim of this review is to specify new potential reliable and non-invasive methods for the diagnosis of biliary atresia (BA) that could shorten the way to diagnose BA, and finally the surgical treatment. Apart from the biomarkers that have been proven helpful and are used nowadays in neonatal wards, there are several new potential biomarkers that researchers have found to be helpful in the diagnosis of biliary atresia. Circulating microRNAs, matrix metalloproteinase-7, stool proteins, interleukin-33, Th17-associated cytokines, urina...
Source: Acta Biochim Pol - September 17, 2023 Category: Biochemistry Authors: Anna Lew-Tusk Marta P ęksa Teresa Stachowicz-Stencel Source Type: research
