LncRNA MIR31HG promotes cell proliferation and invasive properties of the MCF-7 cell line by regulation of receptor-interacting serine-threonine kinase 4
Acta Biochim Pol. 2023 Dec 8. doi: 10.18388/abp.2020_6842. Online ahead of print.ABSTRACTLncRNA MIR31HG is involved in many types of cancers, while its roles in breast cancer are still unknown. The current study aimed to explore the function of lncRNA MIR31HG in breast cancer and the underlying mechanisms. Stable expression cell lines were constructed by using lentivirus particles. MTT assay was used to determine cell viability. Wound healing and Transwell assay were used to determine cell migration and invasion, respectively. The changes in biomarkers were determined by using qPR-PCT and Western blotting, respectively. BA...
Source: Acta Biochim Pol - December 8, 2023 Category: Biochemistry Authors: Jingwei Tang Xiaojing Zhang Chunchun Chen Binbin Wang Yansong Chen Hao Zhang Mengxiang Qiao Xianfu Liu Wei Guo Gongsheng Jin Source Type: research
Mapping the substrate-binding subsite specificity of a Porphyromonas gingivalis Tpr peptidase
Acta Biochim Pol. 2023 Dec 8. doi: 10.18388/abp.2020_6904. Online ahead of print.ABSTRACTCalcium-dependent peptidases of the calpain family are widespread in eukaryotes but uncommon in prokaryotes. A few bacterial calpain homologs have been discovered but none of them have been characterized in detail. Here we present an in-depth substrate specificity analysis of the bacterial calpain-like peptidase Tpr from Porphyromonas gingivalis. Using the positional scanning hybrid combinatorial substrate library method, we found that the specificity of Tpr peptidase differs substantially from the papain family of cysteine proteases, ...
Source: Acta Biochim Pol - December 8, 2023 Category: Biochemistry Authors: Dominika Staniec Wioletta Rut Marcin Drag Michal Burmistrz Michael Kitching Jan Potempa Source Type: research
LncRNA MIR31HG promotes cell proliferation and invasive properties of the MCF-7 cell line by regulation of receptor-interacting serine-threonine kinase 4
Acta Biochim Pol. 2023 Dec 8. doi: 10.18388/abp.2020_6842. Online ahead of print.ABSTRACTLncRNA MIR31HG is involved in many types of cancers, while its roles in breast cancer are still unknown. The current study aimed to explore the function of lncRNA MIR31HG in breast cancer and the underlying mechanisms. Stable expression cell lines were constructed by using lentivirus particles. MTT assay was used to determine cell viability. Wound healing and Transwell assay were used to determine cell migration and invasion, respectively. The changes in biomarkers were determined by using qPR-PCT and Western blotting, respectively. BA...
Source: Acta Biochim Pol - December 8, 2023 Category: Biochemistry Authors: Jingwei Tang Xiaojing Zhang Chunchun Chen Binbin Wang Yansong Chen Hao Zhang Mengxiang Qiao Xianfu Liu Wei Guo Gongsheng Jin Source Type: research
Mapping the substrate-binding subsite specificity of a Porphyromonas gingivalis Tpr peptidase
Acta Biochim Pol. 2023 Dec 8. doi: 10.18388/abp.2020_6904. Online ahead of print.ABSTRACTCalcium-dependent peptidases of the calpain family are widespread in eukaryotes but uncommon in prokaryotes. A few bacterial calpain homologs have been discovered but none of them have been characterized in detail. Here we present an in-depth substrate specificity analysis of the bacterial calpain-like peptidase Tpr from Porphyromonas gingivalis. Using the positional scanning hybrid combinatorial substrate library method, we found that the specificity of Tpr peptidase differs substantially from the papain family of cysteine proteases, ...
Source: Acta Biochim Pol - December 8, 2023 Category: Biochemistry Authors: Dominika Staniec Wioletta Rut Marcin Drag Michal Burmistrz Michael Kitching Jan Potempa Source Type: research
LncRNA MIR31HG promotes cell proliferation and invasive properties of the MCF-7 cell line by regulation of receptor-interacting serine-threonine kinase 4
Acta Biochim Pol. 2023 Dec 8. doi: 10.18388/abp.2020_6842. Online ahead of print.ABSTRACTLncRNA MIR31HG is involved in many types of cancers, while its roles in breast cancer are still unknown. The current study aimed to explore the function of lncRNA MIR31HG in breast cancer and the underlying mechanisms. Stable expression cell lines were constructed by using lentivirus particles. MTT assay was used to determine cell viability. Wound healing and Transwell assay were used to determine cell migration and invasion, respectively. The changes in biomarkers were determined by using qPR-PCT and Western blotting, respectively. BA...
Source: Acta Biochim Pol - December 8, 2023 Category: Biochemistry Authors: Jingwei Tang Xiaojing Zhang Chunchun Chen Binbin Wang Yansong Chen Hao Zhang Mengxiang Qiao Xianfu Liu Wei Guo Gongsheng Jin Source Type: research
Protective effect of tretinoin derivative and TXNRD1 protein on streptozotocin induced gestational diabetes via an age-rage signaling-pathway
CONCLUSION: In conclusion, tretinoin derivative administration effectively regulated streptozotocin-induced changes in fasting blood glucose, insulin level, high-density lipid level and triglyceride level in diabetic mice in vivo. The streptozotocin-induced excessive production of C-reactive protein and inflammatory cytokines was also down-regulated in diabetic mice on administration of tretinoin derivative. Therefore, tretinoin derivative can be investigated further as a therapeutic agent for the treatment of gestational diabetes mellitus.PMID:38060807 | DOI:10.18388/abp.2020_6947 (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - December 7, 2023 Category: Biochemistry Authors: Wensheng Wang Lin Wang Source Type: research
Emerging relationship between hydrogen sulfide and ferroptosis: A literature review
Acta Biochim Pol. 2023 Dec 7. doi: 10.18388/abp.2020_6756. Online ahead of print.ABSTRACTGaseous hydrogen sulfide (H2S) can function as a signaling molecule similar to nitric oxide or carbon monoxide under physiological conditions, ultimately exerting anti-inflammatory, anti-apoptotic, and antioxidant activities. Many studies have investigated the role of H2S in a variety of biological contexts, and both endogenous H2S and H2S donors have been leveraged as tools for fundamental biomedical research, and it has been suggested that they may provide value for the design of novel therapeutic strategies in the years to come. Fer...
Source: Acta Biochim Pol - December 7, 2023 Category: Biochemistry Authors: Xiaoming Gao Ke Lu Chong Li Source Type: research
Value evaluation of serum (sdLDLc*HCYc)/HDLc ratio in the stability of intracranial arterial plaques in patients with acute cerebral infarction
CONCLUSION: The serum (sdLDLc*HCYc)/HDLc ratio was found to have potential in evaluating the stability of intracranial arterial plaques in patients with acute cerebral infarction.PMID:38060820 | DOI:10.18388/abp.2020_6817 (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - December 7, 2023 Category: Biochemistry Authors: Hongyu Hao Xing Xing Yajing Li Hongshan Chu Lei Zhao Siqi Cheng Yang Liu Tiankui Wang Nan Meng Ruisheng Duan Source Type: research
Protective effect of tretinoin derivative and TXNRD1 protein on streptozotocin induced gestational diabetes via an age-rage signaling-pathway
CONCLUSION: In conclusion, tretinoin derivative administration effectively regulated streptozotocin-induced changes in fasting blood glucose, insulin level, high-density lipid level and triglyceride level in diabetic mice in vivo. The streptozotocin-induced excessive production of C-reactive protein and inflammatory cytokines was also down-regulated in diabetic mice on administration of tretinoin derivative. Therefore, tretinoin derivative can be investigated further as a therapeutic agent for the treatment of gestational diabetes mellitus.PMID:38060807 | DOI:10.18388/abp.2020_6947 (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - December 7, 2023 Category: Biochemistry Authors: Wensheng Wang Lin Wang Source Type: research
Emerging relationship between hydrogen sulfide and ferroptosis: A literature review
Acta Biochim Pol. 2023 Dec 7. doi: 10.18388/abp.2020_6756. Online ahead of print.ABSTRACTGaseous hydrogen sulfide (H2S) can function as a signaling molecule similar to nitric oxide or carbon monoxide under physiological conditions, ultimately exerting anti-inflammatory, anti-apoptotic, and antioxidant activities. Many studies have investigated the role of H2S in a variety of biological contexts, and both endogenous H2S and H2S donors have been leveraged as tools for fundamental biomedical research, and it has been suggested that they may provide value for the design of novel therapeutic strategies in the years to come. Fer...
Source: Acta Biochim Pol - December 7, 2023 Category: Biochemistry Authors: Xiaoming Gao Ke Lu Chong Li Source Type: research
Value evaluation of serum (sdLDLc*HCYc)/HDLc ratio in the stability of intracranial arterial plaques in patients with acute cerebral infarction
CONCLUSION: The serum (sdLDLc*HCYc)/HDLc ratio was found to have potential in evaluating the stability of intracranial arterial plaques in patients with acute cerebral infarction.PMID:38060820 | DOI:10.18388/abp.2020_6817 (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - December 7, 2023 Category: Biochemistry Authors: Hongyu Hao Xing Xing Yajing Li Hongshan Chu Lei Zhao Siqi Cheng Yang Liu Tiankui Wang Nan Meng Ruisheng Duan Source Type: research
Modified Hongyu Decoction promotes wound healing by activating the VEGF/PI3K/Akt signaling pathway
This study aims to elucidate the wound healing mechanism of Modified Hongyu Decoction (MHD) in vivo and in vitro. MHD wound healing activity in vivo was evaluated using an excision rat model. H and E staining, Masson's staining and immunofluorescence of wound tissue on days 7 and 14 were performed to evaluate the efficacy of MHD on wound healing. Subsequently, human umbilical vein endothelial cells (HUVECs) were used to evaluate wound healing characteristics in vitro. Cell Counting Kit-8 (CCK-8) and scratch assays were conducted to assess the effects of MHD on the proliferation and migration of HUVECs. The involvement of t...
Source: Acta Biochim Pol - December 5, 2023 Category: Biochemistry Authors: Xiang Xu Wei-Hua Yang Zhi-Wei Miao Chun-Yu Zhang Yi-Jia Cheng Yang Chen Jin-Gen Lu Ning He Source Type: research
Identification of AHNAK expression associated with the pathogenesis of chronic obstructive pulmonary disease by bioinformatic analysis
CONCLUSION: AHNAK may be a feasible biomarker playing crucial functions in the diagnosis and progression of COPD.PMID:38051912 | DOI:10.18388/abp.2020_6041 (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - December 5, 2023 Category: Biochemistry Authors: Chunhui Zhang Yu Liu Source Type: research
Prognostic value of serum albumin level in patients with diffuse large B cell lymphoma
CONCLUSION: SA can be used as an indicator of prognosis in patients with DLBCL before the first chemotherapy. DLBCL patients with SA≤34 g/L are associated with short OS and poor prognosis, which may potentially provide guidance for the clinician to pay more attention to this population before the first chemotherapy.PMID:38051992 | DOI:10.18388/abp.2020_6171 (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - December 5, 2023 Category: Biochemistry Authors: Liyan Chen Lili Pan Tingbo Liu Source Type: research
The cadherin protein CDH19 mediates cervical carcinoma progression by regulating AKT/NF- κB signaling
Acta Biochim Pol. 2023 Dec 5. doi: 10.18388/abp.2020_6902. Online ahead of print.ABSTRACTThe cell adhesion protein cadherin 19 (CDH19) has been reported to be involved in various types of cancer, but its role in cervical carcinoma remains unknown. We collected and analyzed the patients' data using the GEPIA Kaplan-Meier plotter databases. CDH19 was overexpressed in cervical carcinoma cells to assess its effect on cell proliferation and activation of AKT and NF-κB signaling pathways. A xenograft mouse model was established to study the function of CDH19 in vivo. We found that CDH19 expression was significantly downregulate...
Source: Acta Biochim Pol - December 5, 2023 Category: Biochemistry Authors: Jia Yu Xin Sun Yani Yu Xiaorong Cui Source Type: research
