Viable Leishmania parasites in the absence of an in vitro IFN- γ response in asymptomatic carriers
This study aimed to identify the in vitro expression of IFN-γ in asymptomatic carriers of viable Leishmania parasites. Asymptomatic infection was identified using the Montenegro skin test in an at-risk population from Yucatan, Mexico. Parasite viability was evinced in the blood by 7SL RNA transcripts amplification. The expression of mRNA IFN-γ was analyzed in peripheral blood mononuclear cells stimulated with soluble Leishmania antigen, using RT-qPCR. Parasite viability was observed in 33.3 % (5/15) of asymptomatic subjects. No differences were found in the expression of IFN-γ between asymptomatic and healthy subjects, ...
Source: Revista do Instituto de Medicina Tropical de Sao Paulo - February 21, 2024 Category: Tropical Medicine Authors: Elsy Nalleli Lor ía-Cervera Erika Ivett Sosa-Bibiano Karina Beatriz L ópez-Ávila Ana Celia Montes de Oca-Aguilar Marisol Sarah í Moreno-Nava Jimmy Raymundo Torres-Castro Source Type: research

Comparison of conjunctival swabs and blood samples in dogs for molecular and serological detection of Leishmania infantum infection in Colombia
CONCLUSION: The use of conjunctival swab as a non-invasive sample could be used as an alternative method for surveillance of canine leishmaniasis.PMID:38381044 | DOI:10.4103/JVBD.JVBD_182_23 (Source: Journal of Vector Borne Diseases)
Source: Journal of Vector Borne Diseases - February 21, 2024 Category: Infectious Diseases Authors: Mej ía-Chimá Wilmer A Hern ández-Vera Samuel D P érez-Vargas Oscar Y Rivero Rodriguez Matilde E Paternina Luis E Bejarano Eduar E Source Type: research

Viable Leishmania parasites in the absence of an in vitro IFN- γ response in asymptomatic carriers
This study aimed to identify the in vitro expression of IFN-γ in asymptomatic carriers of viable Leishmania parasites. Asymptomatic infection was identified using the Montenegro skin test in an at-risk population from Yucatan, Mexico. Parasite viability was evinced in the blood by 7SL RNA transcripts amplification. The expression of mRNA IFN-γ was analyzed in peripheral blood mononuclear cells stimulated with soluble Leishmania antigen, using RT-qPCR. Parasite viability was observed in 33.3 % (5/15) of asymptomatic subjects. No differences were found in the expression of IFN-γ between asymptomatic and healthy subjects, ...
Source: Revista do Instituto de Medicina Tropical de Sao Paulo - February 21, 2024 Category: Tropical Medicine Authors: Elsy Nalleli Lor ía-Cervera Erika Ivett Sosa-Bibiano Karina Beatriz L ópez-Ávila Ana Celia Montes de Oca-Aguilar Marisol Sarah í Moreno-Nava Jimmy Raymundo Torres-Castro Source Type: research

Comparison of conjunctival swabs and blood samples in dogs for molecular and serological detection of Leishmania infantum infection in Colombia
CONCLUSION: The use of conjunctival swab as a non-invasive sample could be used as an alternative method for surveillance of canine leishmaniasis.PMID:38381044 | DOI:10.4103/JVBD.JVBD_182_23 (Source: Journal of Vector Borne Diseases)
Source: Journal of Vector Borne Diseases - February 21, 2024 Category: Infectious Diseases Authors: Mej ía-Chimá Wilmer A Hern ández-Vera Samuel D P érez-Vargas Oscar Y Rivero Rodriguez Matilde E Paternina Luis E Bejarano Eduar E Source Type: research

Viable Leishmania parasites in the absence of an in vitro IFN- γ response in asymptomatic carriers
This study aimed to identify the in vitro expression of IFN-γ in asymptomatic carriers of viable Leishmania parasites. Asymptomatic infection was identified using the Montenegro skin test in an at-risk population from Yucatan, Mexico. Parasite viability was evinced in the blood by 7SL RNA transcripts amplification. The expression of mRNA IFN-γ was analyzed in peripheral blood mononuclear cells stimulated with soluble Leishmania antigen, using RT-qPCR. Parasite viability was observed in 33.3 % (5/15) of asymptomatic subjects. No differences were found in the expression of IFN-γ between asymptomatic and healthy subjects, ...
Source: Revista do Instituto de Medicina Tropical de Sao Paulo - February 21, 2024 Category: Tropical Medicine Authors: Elsy Nalleli Lor ía-Cervera Erika Ivett Sosa-Bibiano Karina Beatriz L ópez-Ávila Ana Celia Montes de Oca-Aguilar Marisol Sarah í Moreno-Nava Jimmy Raymundo Torres-Castro Source Type: research

Comparison of conjunctival swabs and blood samples in dogs for molecular and serological detection of Leishmania infantum infection in Colombia
CONCLUSION: The use of conjunctival swab as a non-invasive sample could be used as an alternative method for surveillance of canine leishmaniasis.PMID:38381044 | DOI:10.4103/JVBD.JVBD_182_23 (Source: Journal of Vector Borne Diseases)
Source: Journal of Vector Borne Diseases - February 21, 2024 Category: Infectious Diseases Authors: Mej ía-Chimá Wilmer A Hern ández-Vera Samuel D P érez-Vargas Oscar Y Rivero Rodriguez Matilde E Paternina Luis E Bejarano Eduar E Source Type: research

Viable Leishmania parasites in the absence of an in vitro IFN- γ response in asymptomatic carriers
This study aimed to identify the in vitro expression of IFN-γ in asymptomatic carriers of viable Leishmania parasites. Asymptomatic infection was identified using the Montenegro skin test in an at-risk population from Yucatan, Mexico. Parasite viability was evinced in the blood by 7SL RNA transcripts amplification. The expression of mRNA IFN-γ was analyzed in peripheral blood mononuclear cells stimulated with soluble Leishmania antigen, using RT-qPCR. Parasite viability was observed in 33.3 % (5/15) of asymptomatic subjects. No differences were found in the expression of IFN-γ between asymptomatic and healthy subjects, ...
Source: Revista do Instituto de Medicina Tropical de Sao Paulo - February 21, 2024 Category: Tropical Medicine Authors: Elsy Nalleli Lor ía-Cervera Erika Ivett Sosa-Bibiano Karina Beatriz L ópez-Ávila Ana Celia Montes de Oca-Aguilar Marisol Sarah í Moreno-Nava Jimmy Raymundo Torres-Castro Source Type: research

Comparison of conjunctival swabs and blood samples in dogs for molecular and serological detection of Leishmania infantum infection in Colombia
CONCLUSION: The use of conjunctival swab as a non-invasive sample could be used as an alternative method for surveillance of canine leishmaniasis.PMID:38381044 | DOI:10.4103/JVBD.JVBD_182_23 (Source: Journal of Vector Borne Diseases)
Source: Journal of Vector Borne Diseases - February 21, 2024 Category: Infectious Diseases Authors: Mej ía-Chimá Wilmer A Hern ández-Vera Samuel D P érez-Vargas Oscar Y Rivero Rodriguez Matilde E Paternina Luis E Bejarano Eduar E Source Type: research

Viable Leishmania parasites in the absence of an in vitro IFN- γ response in asymptomatic carriers
This study aimed to identify the in vitro expression of IFN-γ in asymptomatic carriers of viable Leishmania parasites. Asymptomatic infection was identified using the Montenegro skin test in an at-risk population from Yucatan, Mexico. Parasite viability was evinced in the blood by 7SL RNA transcripts amplification. The expression of mRNA IFN-γ was analyzed in peripheral blood mononuclear cells stimulated with soluble Leishmania antigen, using RT-qPCR. Parasite viability was observed in 33.3 % (5/15) of asymptomatic subjects. No differences were found in the expression of IFN-γ between asymptomatic and healthy subjects, ...
Source: Revista do Instituto de Medicina Tropical de Sao Paulo - February 21, 2024 Category: Tropical Medicine Authors: Elsy Nalleli Lor ía-Cervera Erika Ivett Sosa-Bibiano Karina Beatriz L ópez-Ávila Ana Celia Montes de Oca-Aguilar Marisol Sarah í Moreno-Nava Jimmy Raymundo Torres-Castro Source Type: research

Comparison of conjunctival swabs and blood samples in dogs for molecular and serological detection of Leishmania infantum infection in Colombia
CONCLUSION: The use of conjunctival swab as a non-invasive sample could be used as an alternative method for surveillance of canine leishmaniasis.PMID:38381044 | DOI:10.4103/JVBD.JVBD_182_23 (Source: Journal of Vector Borne Diseases)
Source: Journal of Vector Borne Diseases - February 21, 2024 Category: Infectious Diseases Authors: Mej ía-Chimá Wilmer A Hern ández-Vera Samuel D P érez-Vargas Oscar Y Rivero Rodriguez Matilde E Paternina Luis E Bejarano Eduar E Source Type: research

Viable Leishmania parasites in the absence of an in vitro IFN- γ response in asymptomatic carriers
This study aimed to identify the in vitro expression of IFN-γ in asymptomatic carriers of viable Leishmania parasites. Asymptomatic infection was identified using the Montenegro skin test in an at-risk population from Yucatan, Mexico. Parasite viability was evinced in the blood by 7SL RNA transcripts amplification. The expression of mRNA IFN-γ was analyzed in peripheral blood mononuclear cells stimulated with soluble Leishmania antigen, using RT-qPCR. Parasite viability was observed in 33.3 % (5/15) of asymptomatic subjects. No differences were found in the expression of IFN-γ between asymptomatic and healthy subjects, ...
Source: Revista do Instituto de Medicina Tropical de Sao Paulo - February 21, 2024 Category: Tropical Medicine Authors: Elsy Nalleli Lor ía-Cervera Erika Ivett Sosa-Bibiano Karina Beatriz L ópez-Ávila Ana Celia Montes de Oca-Aguilar Marisol Sarah í Moreno-Nava Jimmy Raymundo Torres-Castro Source Type: research

Comparison of conjunctival swabs and blood samples in dogs for molecular and serological detection of Leishmania infantum infection in Colombia
CONCLUSION: The use of conjunctival swab as a non-invasive sample could be used as an alternative method for surveillance of canine leishmaniasis.PMID:38381044 | DOI:10.4103/JVBD.JVBD_182_23 (Source: Journal of Vector Borne Diseases)
Source: Journal of Vector Borne Diseases - February 21, 2024 Category: Infectious Diseases Authors: Mej ía-Chimá Wilmer A Hern ández-Vera Samuel D P érez-Vargas Oscar Y Rivero Rodriguez Matilde E Paternina Luis E Bejarano Eduar E Source Type: research

Viable Leishmania parasites in the absence of an in vitro IFN- γ response in asymptomatic carriers
This study aimed to identify the in vitro expression of IFN-γ in asymptomatic carriers of viable Leishmania parasites. Asymptomatic infection was identified using the Montenegro skin test in an at-risk population from Yucatan, Mexico. Parasite viability was evinced in the blood by 7SL RNA transcripts amplification. The expression of mRNA IFN-γ was analyzed in peripheral blood mononuclear cells stimulated with soluble Leishmania antigen, using RT-qPCR. Parasite viability was observed in 33.3 % (5/15) of asymptomatic subjects. No differences were found in the expression of IFN-γ between asymptomatic and healthy subjects, ...
Source: Revista do Instituto de Medicina Tropical de Sao Paulo - February 21, 2024 Category: Tropical Medicine Authors: Elsy Nalleli Lor ía-Cervera Erika Ivett Sosa-Bibiano Karina Beatriz L ópez-Ávila Ana Celia Montes de Oca-Aguilar Marisol Sarah í Moreno-Nava Jimmy Raymundo Torres-Castro Source Type: research

Comparison of conjunctival swabs and blood samples in dogs for molecular and serological detection of Leishmania infantum infection in Colombia
CONCLUSION: The use of conjunctival swab as a non-invasive sample could be used as an alternative method for surveillance of canine leishmaniasis.PMID:38381044 | DOI:10.4103/JVBD.JVBD_182_23 (Source: Journal of Vector Borne Diseases)
Source: Journal of Vector Borne Diseases - February 21, 2024 Category: Infectious Diseases Authors: Mej ía-Chimá Wilmer A Hern ández-Vera Samuel D P érez-Vargas Oscar Y Rivero Rodriguez Matilde E Paternina Luis E Bejarano Eduar E Source Type: research

Viable Leishmania parasites in the absence of an in vitro IFN- γ response in asymptomatic carriers
This study aimed to identify the in vitro expression of IFN-γ in asymptomatic carriers of viable Leishmania parasites. Asymptomatic infection was identified using the Montenegro skin test in an at-risk population from Yucatan, Mexico. Parasite viability was evinced in the blood by 7SL RNA transcripts amplification. The expression of mRNA IFN-γ was analyzed in peripheral blood mononuclear cells stimulated with soluble Leishmania antigen, using RT-qPCR. Parasite viability was observed in 33.3 % (5/15) of asymptomatic subjects. No differences were found in the expression of IFN-γ between asymptomatic and healthy subjects, ...
Source: Revista do Instituto de Medicina Tropical de Sao Paulo - February 21, 2024 Category: Tropical Medicine Authors: Elsy Nalleli Lor ía-Cervera Erika Ivett Sosa-Bibiano Karina Beatriz L ópez-Ávila Ana Celia Montes de Oca-Aguilar Marisol Sarah í Moreno-Nava Jimmy Raymundo Torres-Castro Source Type: research