Substrate stiffness modulates cardiac fibroblast activation, senescence and proinflammatory secretory phenotype
Am J Physiol Heart Circ Physiol. 2023 Oct 27. doi: 10.1152/ajpheart.00483.2023. Online ahead of print.ABSTRACTIn vitro cultures of primary cardiac fibroblasts (CFs), the major extracellular matrix (ECM)-producing cells of the heart, are used to determine molecular mechanisms of cardiac fibrosis. However, the supraphysiologic stiffness of tissue culture polystyrene (TCPS) triggers conversion of CFs into an activated myofibroblast-like state, and serial passage of the cells results in the induction of replicative senescence. These phenotypic switches confound interpretation of experimental data obtained with cultured CFs. In...
Source: American Journal of Physiology. Heart and Circulatory Physiology - October 27, 2023 Category: Physiology Authors: Marina B Felisbino Marcello Rubino Joshua G Travers Katherine B Schuetze Madeleine E Lemieux Kristi S Anseth Brian A Aguado Timothy A McKinsey Source Type: research
Substrate stiffness modulates cardiac fibroblast activation, senescence and proinflammatory secretory phenotype
Am J Physiol Heart Circ Physiol. 2023 Oct 27. doi: 10.1152/ajpheart.00483.2023. Online ahead of print.ABSTRACTIn vitro cultures of primary cardiac fibroblasts (CFs), the major extracellular matrix (ECM)-producing cells of the heart, are used to determine molecular mechanisms of cardiac fibrosis. However, the supraphysiologic stiffness of tissue culture polystyrene (TCPS) triggers conversion of CFs into an activated myofibroblast-like state, and serial passage of the cells results in the induction of replicative senescence. These phenotypic switches confound interpretation of experimental data obtained with cultured CFs. In...
Source: American Journal of Physiology. Heart and Circulatory Physiology - October 27, 2023 Category: Physiology Authors: Marina B Felisbino Marcello Rubino Joshua G Travers Katherine B Schuetze Madeleine E Lemieux Kristi S Anseth Brian A Aguado Timothy A McKinsey Source Type: research
Substrate stiffness modulates cardiac fibroblast activation, senescence and proinflammatory secretory phenotype
Am J Physiol Heart Circ Physiol. 2023 Oct 27. doi: 10.1152/ajpheart.00483.2023. Online ahead of print.ABSTRACTIn vitro cultures of primary cardiac fibroblasts (CFs), the major extracellular matrix (ECM)-producing cells of the heart, are used to determine molecular mechanisms of cardiac fibrosis. However, the supraphysiologic stiffness of tissue culture polystyrene (TCPS) triggers conversion of CFs into an activated myofibroblast-like state, and serial passage of the cells results in the induction of replicative senescence. These phenotypic switches confound interpretation of experimental data obtained with cultured CFs. In...
Source: American Journal of Physiology. Heart and Circulatory Physiology - October 27, 2023 Category: Physiology Authors: Marina B Felisbino Marcello Rubino Joshua G Travers Katherine B Schuetze Madeleine E Lemieux Kristi S Anseth Brian A Aguado Timothy A McKinsey Source Type: research
Substrate stiffness modulates cardiac fibroblast activation, senescence and proinflammatory secretory phenotype
Am J Physiol Heart Circ Physiol. 2023 Oct 27. doi: 10.1152/ajpheart.00483.2023. Online ahead of print.ABSTRACTIn vitro cultures of primary cardiac fibroblasts (CFs), the major extracellular matrix (ECM)-producing cells of the heart, are used to determine molecular mechanisms of cardiac fibrosis. However, the supraphysiologic stiffness of tissue culture polystyrene (TCPS) triggers conversion of CFs into an activated myofibroblast-like state, and serial passage of the cells results in the induction of replicative senescence. These phenotypic switches confound interpretation of experimental data obtained with cultured CFs. In...
Source: American Journal of Physiology. Heart and Circulatory Physiology - October 27, 2023 Category: Physiology Authors: Marina B Felisbino Marcello Rubino Joshua G Travers Katherine B Schuetze Madeleine E Lemieux Kristi S Anseth Brian A Aguado Timothy A McKinsey Source Type: research
Substrate stiffness modulates cardiac fibroblast activation, senescence and proinflammatory secretory phenotype
Am J Physiol Heart Circ Physiol. 2023 Oct 27. doi: 10.1152/ajpheart.00483.2023. Online ahead of print.ABSTRACTIn vitro cultures of primary cardiac fibroblasts (CFs), the major extracellular matrix (ECM)-producing cells of the heart, are used to determine molecular mechanisms of cardiac fibrosis. However, the supraphysiologic stiffness of tissue culture polystyrene (TCPS) triggers conversion of CFs into an activated myofibroblast-like state, and serial passage of the cells results in the induction of replicative senescence. These phenotypic switches confound interpretation of experimental data obtained with cultured CFs. In...
Source: American Journal of Physiology. Heart and Circulatory Physiology - October 27, 2023 Category: Physiology Authors: Marina B Felisbino Marcello Rubino Joshua G Travers Katherine B Schuetze Madeleine E Lemieux Kristi S Anseth Brian A Aguado Timothy A McKinsey Source Type: research
Substrate stiffness modulates cardiac fibroblast activation, senescence and proinflammatory secretory phenotype
Am J Physiol Heart Circ Physiol. 2023 Oct 27. doi: 10.1152/ajpheart.00483.2023. Online ahead of print.ABSTRACTIn vitro cultures of primary cardiac fibroblasts (CFs), the major extracellular matrix (ECM)-producing cells of the heart, are used to determine molecular mechanisms of cardiac fibrosis. However, the supraphysiologic stiffness of tissue culture polystyrene (TCPS) triggers conversion of CFs into an activated myofibroblast-like state, and serial passage of the cells results in the induction of replicative senescence. These phenotypic switches confound interpretation of experimental data obtained with cultured CFs. In...
Source: American Journal of Physiology. Heart and Circulatory Physiology - October 27, 2023 Category: Physiology Authors: Marina B Felisbino Marcello Rubino Joshua G Travers Katherine B Schuetze Madeleine E Lemieux Kristi S Anseth Brian A Aguado Timothy A McKinsey Source Type: research
Multiple craniotomies for the resection of symptomatic multifocal intracranial metastatic cardiac myxoma: A case report
CONCLUSION: Although rare, hematogenous seeding with subsequent formation of hemorrhagic metastasis is a possible complication of atrial myxoma. While surgical resection, radiation therapy, and chemotherapy have historically been used, no standard of care currently exists. This case demonstrates repeat tumor resection as effective for managing symptomatic intracranial metastatic myxoma in a patient with poor response to radiation therapy and multiple recurrences, with follow-up showing improvement in neurological symptoms and mass effect and absence of recurrence on imaging.PMID:37810324 | PMC:PMC10559549 | DOI:10.25259/SN...
Source: Surgical Neurology International - October 9, 2023 Category: Neurosurgery Authors: V Peter Abdow Iii Jeffrey M Breton Vikram V Nayar Source Type: research
