Mannose controls mesoderm specification and symmetry breaking in mouse gastruloids
Dev Cell. 2024 Apr 9:S1534-5807(24)00198-9. doi: 10.1016/j.devcel.2024.03.031. Online ahead of print.ABSTRACTPatterning and growth are fundamental features of embryonic development that must be tightly coordinated. To understand how metabolism impacts early mesoderm development, we used mouse embryonic stem-cell-derived gastruloids, that co-expressed glucose transporters with the mesodermal marker T/Bra. We found that the glucose mimic, 2-deoxy-D-glucose (2-DG), blocked T/Bra expression and abolished axial elongation in gastruloids. However, glucose removal did not phenocopy 2-DG treatment despite a decline in glycolytic i...
Source: Developmental Cell - April 18, 2024 Category: Cytology Authors: Chaitanya Dingare Dominica Cao Jenny Jingni Yang Berna Sozen Benjamin Steventon Source Type: research
Mannose controls mesoderm specification and symmetry breaking in mouse gastruloids
Dev Cell. 2024 Apr 9:S1534-5807(24)00198-9. doi: 10.1016/j.devcel.2024.03.031. Online ahead of print.ABSTRACTPatterning and growth are fundamental features of embryonic development that must be tightly coordinated. To understand how metabolism impacts early mesoderm development, we used mouse embryonic stem-cell-derived gastruloids, that co-expressed glucose transporters with the mesodermal marker T/Bra. We found that the glucose mimic, 2-deoxy-D-glucose (2-DG), blocked T/Bra expression and abolished axial elongation in gastruloids. However, glucose removal did not phenocopy 2-DG treatment despite a decline in glycolytic i...
Source: Developmental Cell - April 18, 2024 Category: Cytology Authors: Chaitanya Dingare Dominica Cao Jenny Jingni Yang Berna Sozen Benjamin Steventon Source Type: research
Cancers, Vol. 16, Pages 1532: Lysine Methyltransferase 9 (KMT9) Is an Actionable Target in Muscle-Invasive Bladder Cancer
In this study, we show that KMT9 regulates proliferation, migration, and invasion of various MIBC cell lines with different genetic mutations. KMT9α depletion results in the differential expression of genes regulating the cell cycle, cell adhesion, and migration. Differentially expressed genes include oncogenes such as EGFR and AKT1 as well as mediators of cell adhesion or migration such as DAG1 and ITGA6. Reduced cell proliferation upon KMT9α depletion is also observed in Pten/Trp53 knockout bladder tumor organoids, which cannot be rescued with an enzymatically inactive KMT9α muta...
Source: Cancers - April 17, 2024 Category: Cancer & Oncology Authors: Sainab Totonji Anna Ramos-Triguero Dominica Willmann Manuela Sum Sylvia Urban Helena Bauer Astrid Rieder Sheng Wang Holger Greschik Eric Metzger Roland Sch üle Tags: Article Source Type: research
Characteristics and Outcomes of a Single-Centre Cohort of Adult Congenital Heart Disease Patients Referred for Heart Transplant
Adult congenital heart disease (ACHD) services increasingly encounter heart failure (HF) in the ageing ACHD population. Optimal timing of referral for heart transplant (HTx) evaluation in this heterogeneous population is complex and ill-defined. We aim to outline the characteristics and outcomes of ACHD patients referred for HTx from a large Australian ACHD centre. (Source: Heart, Lung and Circulation)
Source: Heart, Lung and Circulation - April 11, 2024 Category: Cardiology Authors: Pavithra S. Jayadeva, Stacey Peters, Su Ling Tee, Luke J. Burchill, Silvana F. Marasco, Leeanne Grigg, Angeline Leet, David McGiffin, Dominica Zentner Tags: Original Article Source Type: research
Exploring how a patient encounter tracking and learning tool is used within general practice training: a qualitative study
Discussion The findings were used to develop a Structure-Process-Outcomes model to demonstrate how ReCEnT is currently used and explores how it can be used for learning, rather than of learning, in a programmatic assessment framework for GP training. ReCEnT's longitudinal format has potential for enhancing learning throughout training.PMID:38546773 | DOI:10.1071/HC23082 (Source: Journal of Primary Health Care)
Source: Journal of Primary Health Care - March 28, 2024 Category: Primary Care Authors: Michael Bentley Jennifer Taylor Alison Fielding Andrew Davey Dominica Moad Mieke van Driel Parker Magin Linda Klein Source Type: research
Perceptions of the effectiveness of using patient encounter data as an education and reflection tool in general practice training
Discussion ReCEnT can prompt self-reflection among registrars, leading to changes in clinical practice, learning approaches and training plans. Reaching its potential as an assessment for learning (as opposed to an assessment of learning) requires effective engagement between registrars, their supervisors and MEs.PMID:38546767 | DOI:10.1071/HC22158 (Source: Journal of Primary Health Care)
Source: Journal of Primary Health Care - March 28, 2024 Category: Primary Care Authors: Linda Klein Michael Bentley Dominica Moad Alison Fielding Amanda Tapley Mieke van Driel Andrew Davey Ben Mundy Kristen FitzGerald Jennifer Taylor Racheal Norris Elizabeth Holliday Parker Magin Source Type: research
Exploring how a patient encounter tracking and learning tool is used within general practice training: a qualitative study
Discussion The findings were used to develop a Structure-Process-Outcomes model to demonstrate how ReCEnT is currently used and explores how it can be used for learning, rather than of learning, in a programmatic assessment framework for GP training. ReCEnT's longitudinal format has potential for enhancing learning throughout training.PMID:38546773 | DOI:10.1071/HC23082 (Source: Journal of Primary Health Care)
Source: Journal of Primary Health Care - March 28, 2024 Category: Primary Care Authors: Michael Bentley Jennifer Taylor Alison Fielding Andrew Davey Dominica Moad Mieke van Driel Parker Magin Linda Klein Source Type: research
Perceptions of the effectiveness of using patient encounter data as an education and reflection tool in general practice training
Discussion ReCEnT can prompt self-reflection among registrars, leading to changes in clinical practice, learning approaches and training plans. Reaching its potential as an assessment for learning (as opposed to an assessment of learning) requires effective engagement between registrars, their supervisors and MEs.PMID:38546767 | DOI:10.1071/HC22158 (Source: Journal of Primary Health Care)
Source: Journal of Primary Health Care - March 28, 2024 Category: Primary Care Authors: Linda Klein Michael Bentley Dominica Moad Alison Fielding Amanda Tapley Mieke van Driel Andrew Davey Ben Mundy Kristen FitzGerald Jennifer Taylor Racheal Norris Elizabeth Holliday Parker Magin Source Type: research
Exploring how a patient encounter tracking and learning tool is used within general practice training: a qualitative study
Discussion The findings were used to develop a Structure-Process-Outcomes model to demonstrate how ReCEnT is currently used and explores how it can be used for learning, rather than of learning, in a programmatic assessment framework for GP training. ReCEnT's longitudinal format has potential for enhancing learning throughout training.PMID:38546773 | DOI:10.1071/HC23082 (Source: Journal of Primary Health Care)
Source: Journal of Primary Health Care - March 28, 2024 Category: Primary Care Authors: Michael Bentley Jennifer Taylor Alison Fielding Andrew Davey Dominica Moad Mieke van Driel Parker Magin Linda Klein Source Type: research
Perceptions of the effectiveness of using patient encounter data as an education and reflection tool in general practice training
Discussion ReCEnT can prompt self-reflection among registrars, leading to changes in clinical practice, learning approaches and training plans. Reaching its potential as an assessment for learning (as opposed to an assessment of learning) requires effective engagement between registrars, their supervisors and MEs.PMID:38546767 | DOI:10.1071/HC22158 (Source: Journal of Primary Health Care)
Source: Journal of Primary Health Care - March 28, 2024 Category: Primary Care Authors: Linda Klein Michael Bentley Dominica Moad Alison Fielding Amanda Tapley Mieke van Driel Andrew Davey Ben Mundy Kristen FitzGerald Jennifer Taylor Racheal Norris Elizabeth Holliday Parker Magin Source Type: research
Exploring how a patient encounter tracking and learning tool is used within general practice training: a qualitative study
Discussion The findings were used to develop a Structure-Process-Outcomes model to demonstrate how ReCEnT is currently used and explores how it can be used for learning, rather than of learning, in a programmatic assessment framework for GP training. ReCEnT's longitudinal format has potential for enhancing learning throughout training.PMID:38546773 | DOI:10.1071/HC23082 (Source: Journal of Primary Health Care)
Source: Journal of Primary Health Care - March 28, 2024 Category: Primary Care Authors: Michael Bentley Jennifer Taylor Alison Fielding Andrew Davey Dominica Moad Mieke van Driel Parker Magin Linda Klein Source Type: research
Perceptions of the effectiveness of using patient encounter data as an education and reflection tool in general practice training
Discussion ReCEnT can prompt self-reflection among registrars, leading to changes in clinical practice, learning approaches and training plans. Reaching its potential as an assessment for learning (as opposed to an assessment of learning) requires effective engagement between registrars, their supervisors and MEs.PMID:38546767 | DOI:10.1071/HC22158 (Source: Journal of Primary Health Care)
Source: Journal of Primary Health Care - March 28, 2024 Category: Primary Care Authors: Linda Klein Michael Bentley Dominica Moad Alison Fielding Amanda Tapley Mieke van Driel Andrew Davey Ben Mundy Kristen FitzGerald Jennifer Taylor Racheal Norris Elizabeth Holliday Parker Magin Source Type: research
Exploring how a patient encounter tracking and learning tool is used within general practice training: a qualitative study
Discussion The findings were used to develop a Structure-Process-Outcomes model to demonstrate how ReCEnT is currently used and explores how it can be used for learning, rather than of learning, in a programmatic assessment framework for GP training. ReCEnT's longitudinal format has potential for enhancing learning throughout training.PMID:38546773 | DOI:10.1071/HC23082 (Source: Journal of Primary Health Care)
Source: Journal of Primary Health Care - March 28, 2024 Category: Primary Care Authors: Michael Bentley Jennifer Taylor Alison Fielding Andrew Davey Dominica Moad Mieke van Driel Parker Magin Linda Klein Source Type: research
Perceptions of the effectiveness of using patient encounter data as an education and reflection tool in general practice training
Discussion ReCEnT can prompt self-reflection among registrars, leading to changes in clinical practice, learning approaches and training plans. Reaching its potential as an assessment for learning (as opposed to an assessment of learning) requires effective engagement between registrars, their supervisors and MEs.PMID:38546767 | DOI:10.1071/HC22158 (Source: Journal of Primary Health Care)
Source: Journal of Primary Health Care - March 28, 2024 Category: Primary Care Authors: Linda Klein Michael Bentley Dominica Moad Alison Fielding Amanda Tapley Mieke van Driel Andrew Davey Ben Mundy Kristen FitzGerald Jennifer Taylor Racheal Norris Elizabeth Holliday Parker Magin Source Type: research
