Bacillus subtilis and saponin shifted the availability of heavy metals, health indicators of smelter contaminated soil, and the physiological indicators of Symphytum officinale
Chemosphere. 2021 Jul 12;285:131454. doi: 10.1016/j.chemosphere.2021.131454. Online ahead of print.ABSTRACTBacillus subtilis and saponin were tested for the uptake of heavy metals (HMs) by Symphytum officinale grown in a smelter-contaminated soil in completely randomized design. Soil pH and electrical conductivity increased by 0.11 unit (T3) and 754 mS cm-1 (T2), respectively. The bioavailable Zn decreased by 5.80% (T2); Cd and Pb increased by 6.21% (T2) and 13.46% (T3), respectively. Soil urease increased by 24% (T3) and alkaline phosphatase, β-glucosidase, and dehydrogenase decreased by 20% (T2), 27.70% (T2), and 21% (T...
Source: Chemosphere - July 16, 2021 Category: Chemistry Authors: Yiman Li Amjad Ali Parimala Gnana Soundari Arockiam Jeyasundar Muhammad Azeem Anum Tabassum Di Guo Ronghua Li Ishaq Ahmad Mian Zengqiang Zhang Source Type: research
Bacillus subtilis and saponin shifted the availability of heavy metals, health indicators of smelter contaminated soil, and the physiological indicators of Symphytum officinale
Chemosphere. 2021 Jul 12;285:131454. doi: 10.1016/j.chemosphere.2021.131454. Online ahead of print.ABSTRACTBacillus subtilis and saponin were tested for the uptake of heavy metals (HMs) by Symphytum officinale grown in a smelter-contaminated soil in completely randomized design. Soil pH and electrical conductivity increased by 0.11 unit (T3) and 754 mS cm-1 (T2), respectively. The bioavailable Zn decreased by 5.80% (T2); Cd and Pb increased by 6.21% (T2) and 13.46% (T3), respectively. Soil urease increased by 24% (T3) and alkaline phosphatase, β-glucosidase, and dehydrogenase decreased by 20% (T2), 27.70% (T2), and 21% (T...
Source: Chemosphere - July 16, 2021 Category: Chemistry Authors: Yiman Li Amjad Ali Parimala Gnana Soundari Arockiam Jeyasundar Muhammad Azeem Anum Tabassum Di Guo Ronghua Li Ishaq Ahmad Mian Zengqiang Zhang Source Type: research
Bacillus subtilis and saponin shifted the availability of heavy metals, health indicators of smelter contaminated soil, and the physiological indicators of Symphytum officinale
Chemosphere. 2021 Jul 12;285:131454. doi: 10.1016/j.chemosphere.2021.131454. Online ahead of print.ABSTRACTBacillus subtilis and saponin were tested for the uptake of heavy metals (HMs) by Symphytum officinale grown in a smelter-contaminated soil in completely randomized design. Soil pH and electrical conductivity increased by 0.11 unit (T3) and 754 mS cm-1 (T2), respectively. The bioavailable Zn decreased by 5.80% (T2); Cd and Pb increased by 6.21% (T2) and 13.46% (T3), respectively. Soil urease increased by 24% (T3) and alkaline phosphatase, β-glucosidase, and dehydrogenase decreased by 20% (T2), 27.70% (T2), and 21% (T...
Source: Chemosphere - July 16, 2021 Category: Chemistry Authors: Yiman Li Amjad Ali Parimala Gnana Soundari Arockiam Jeyasundar Muhammad Azeem Anum Tabassum Di Guo Ronghua Li Ishaq Ahmad Mian Zengqiang Zhang Source Type: research
Support for Regulatory Assessment of Percutaneous Absorption of Retronecine-type Pyrrolizidine Alkaloids through Human Skin
This study investigated to what extent pyrrolizidine alkaloids are bioavailable following topical exposure, assessing penetration of retronecine-type PAs in an in vitro human skin model. A single comfrey root formulation was spiked with 3 different congeners (a 7R-monoester, an open-chained 7R-diester, and a cyclic diester) and percutaneous absorption measured per OECD guidelines and good laboratory practices. The measured
penetration for all 3 PAs was low and compared favourably with existing in vitro data. Although consideration of different regulatory guidance influences the determination of dermally absorbed dose,...
Source: Planta Medica - June 11, 2021 Category: Drugs & Pharmacology Authors: Plaza, Alberto Toner, Frank Harris, James Ottersbach, Peter Roper, Clive Mahony, Catherine Tags: Original Papers Source Type: research
Molecules, Vol. 26, Pages 1498: CRISPR/Cas9-Mediated Genome Editing in Comfrey (Symphytum officinale) Hairy Roots Results in the Complete Eradication of Pyrrolizidine Alkaloids
Ober
Comfrey (Symphytum officinale) is a medicinal plant with anti-inflammatory, analgesic, and proliferative properties. However, its pharmaceutical application is hampered by the co-occurrence of toxic pyrrolizidine alkaloids (PAs) in its tissues. Using a CRISPR/Cas9-based approach, we introduced detrimental mutations into the hss gene encoding homospermidine synthase (HSS), the first pathway-specific enzyme of PA biosynthesis. The resulting hairy root (HR) lines were analyzed for the type of gene-editing effect that they exhibited and for their homospermidine and PA content. Inactivation of only one of the two hss ...
Source: Molecules - March 10, 2021 Category: Chemistry Authors: Mahmoud M. Zakaria Brigitte Schemmerling Dietrich Ober Tags: Article Source Type: research
GSE147575 Transcriptional response to a dietary Comfrey supplement in broiler chickens
Contributors : Michael Oster ; Henry Reyer ; Siriluck Ponsuksili ; Klaus WimmersSeries Type : Expression profiling by arrayOrganism : Gallus gallusin vivo microarray study of transcriptional changes in chicken whole blood cells due to dietary intake of Comfrey (Symphytum spp.) (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - December 31, 2020 Category: Genetics & Stem Cells Tags: Expression profiling by array Gallus gallus Source Type: research
Safety of medicinal comfrey cream preparations (Symphytum officinale s.l.): The pyrrolizidine alkaloid lycopsamine is poorly absorbed through human skin
Publication date: Available online 15 September 2020Source: Regulatory Toxicology and PharmacologyAuthor(s): Kenny Kuchta, Mathias Schmidt (Source: Regulatory Toxicology and Pharmacology)
Source: Regulatory Toxicology and Pharmacology - September 15, 2020 Category: Toxicology Source Type: research
Safety of medicinal comfrey cream preparations (Symphytum officinale s.l.): The pyrrolizidine alkaloid lycopsamine is poorly absorbed through human skin.
In this study, lycopsamine served as a model substance for measuring the extent of skin permeation of PAs following the application of a spiked comfrey cream (Symphytum officinale s.l.) to abdominal skin from human donors in Franz diffusion cells. PAs could be excluded in the non-spiked cream with a limit of detection of 8 μg/kg. Only small amounts of the applied quantity of lycopsamine had migrated through the skin sample into the receptor cell side of the diffusion cell after 24 h. In five of six diffusion cells, there was no detectable lycopsamine within the skin and only 0.6 ± 0.4% of the applied dose in the ...
Source: Regulatory Toxicology and Pharmacology : RTP - September 13, 2020 Category: Toxicology Authors: Kuchta K, Schmidt M Tags: Regul Toxicol Pharmacol Source Type: research
Symphytum officinale L.: Liquid-liquid chromatography isolation of caffeic acid oligomers and evaluation of their influence on pro-inflammatory cytokine release in LPS-stimulated neutrophils
Publication date: Available online 31 July 2020Source: Journal of EthnopharmacologyAuthor(s): Adriana Trifan, Krystyna Skalicka-Woźniak, Sebastian Granica, Monika E. Czerwińska, Aleksandra Kruk, Laurence Marcourt, Jean-Luc Wolfender, Evelyn Wolfram, Nils Esslinger, Andreas Grubelnik, Simon Vlad Luca (Source: Journal of Ethnopharmacology)
Source: Journal of Ethnopharmacology - August 2, 2020 Category: Drugs & Pharmacology Source Type: research
Epidermal Regeneration Induced by Comfrey Extract: A Study by Light and Electron Microscopy
Conclusions: Application of comfrey cream led to a quicker regeneration of skin cells and to an earlier differentiation of the cells towards a normal fine structure with a visible distinction of epidermal strata, keratin, and corneocyte formation within 4 –7 days. The study covered the early days of skin regeneration and confirms the benefits observed in published clinical trials and non-interventional studies in patients with abrasions.Skin Pharmacol Physiol (Source: Skin Pharmacology and Physiology)
Source: Skin Pharmacology and Physiology - July 17, 2020 Category: Dermatology Source Type: research
A systematic review and quality assessment of case reports of adverse events for borage (Borago officinalis), coltsfoot (Tussilago farfara) and comfrey (Symphytum officinale).
CONCLUSION: These cases are an unreliable body of evidence on which to draw conclusions about oral consumption of Symphytum officinale and Tussilago farfara. With insufficient evidence to differentiate the relative risk of one PA-containing plant from another; toxicological studies based on oral ingestion of phytochemically complex preparations of these herbs may be the most accurate methodology for assessing clinical risk.
PMID: 32105669 [PubMed - as supplied by publisher] (Source: Fitoterapia)
Source: Fitoterapia - February 23, 2020 Category: Biochemistry Authors: Avila C, Breakspear I, Hawrelak J, Salmond S, Evans S Tags: Fitoterapia Source Type: research
Molecules, Vol. 25, Pages 837: Comparative Assessment of Phytochemical Profiles of Comfrey (Symphytum officinale L.) Root Extracts Obtained by Different Extraction Techniques
arretero
In this work a comparative study on phytochemical profiles of comfrey root extracts obtained by different extraction approaches has been carried out. Chemical profiles of extracts obtained by supercritical fluid (SFE), pressurized liquid (PLE), and conventional solid/liquid extraction were compared and discussed. Phytochemical composition was assessed by high-performance liquid chromatography coupled with electrospray time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS/MS) identifying 39 compounds reported for the first time in comfrey root, mainly phenolic acids and fatty acids. The influence of different extr...
Source: Molecules - February 13, 2020 Category: Chemistry Authors: Nasti ć Borr ás-Linares Lozano-S ánchez Švarc-Gajić Segura-Carretero Tags: Article Source Type: research
TLC-densitometric analysis of allantoin in Symphytum officinale L. roots.
Abstract
A TLC-densitometric method for determination of allantoin in Symphytum officinale root was developed. Densitometric quantification of allantoin was carried out on TLC Si60 plates with butanol-50 % methanol/formic acid, 66.5:33.2:0.3 (V/V/V) as developing solvent, at a wavelength of 190 nm. The method was preliminarily validated in terms of specificity, linearity, precision, limit of detection, limit of quantification, recovery and robustness. The results of TLC quantification were compared with HPLC analysis carried out on a HILIC Luna NH2 100A column, with mobile phase consisting of acetonitrile/...
Source: Acta Pharmaceutica - November 6, 2019 Category: Drugs & Pharmacology Authors: Kimel K, Zienkiewicz M, Sparzak-Stefanowska B, Krauze-Baranowska M Tags: Acta Pharm Source Type: research
Symphytum officinale augments osteogenesis in human bone marrow-derived mesenchymal stem cells in vitro as they differentiate into osteoblasts
ConclusionsOur findings suggest that homoeopathic dose (specially mother tincture) of Symphytum officinale has the potential to enhance osteogenesis.Graphical abstract (Source: Journal of Ethnopharmacology)
Source: Journal of Ethnopharmacology - October 30, 2019 Category: Drugs & Pharmacology Source Type: research
Efficient Extraction of Pyrrolizidine Alkaloids from Plants by Pressurised Liquid Extraction – A Preliminary Study
In this study, we have applied pressurized liquid extraction with the aim of evaluating the effect of various parameters on the recovery of pyrrolizidine alkaloids. The nature of the modifier (various acids, NH3) added to the aqueous extraction solvent, its concentration (1 or 5%), and the temperature (50 – 125 °C) were systematically varied. To analyse a wide range of structurally different pyrrolizidine alkaloids, Jacobaea vulgaris (syn. Senecio jacobaea), Tussilago farfara, and Symphytum officinale were included. Pyrrolizidine alkaloids were quantified by HPLC-MS/MS and the results obtained by pressurised liqui...
Source: Planta Medica - October 20, 2019 Category: Drugs & Pharmacology Authors: Kopp, Thomas Salzer, Liesa Abdel-Tawab, Mona Mizaikoff, Boris Tags: Original Papers Source Type: research
