Detection of metallo-beta-lactamase-producing genes < em > bla < /em > < sub > SPM < /sub > and < em > bla < /em > < sub > NDM < /sub > in < em > Pseudomonas aeruginosa < /em > isolated from wastewater in Southern Brazil
The objective of this study was to investigate genes encoding metallo-beta-lactamases (MBLs), which confer resistance to carbapenems, in wastewater. Fifteen isolates of P. aeruginosa were collected for five months from samples obtained from a municipal wastewater treatment plant in Rio Grande do Sul. These isolates were subjected to disk diffusion testing using 10 different antimicrobials. Phenotypic enzymatic tests for MBLs were conducted, and positive isolates underwent DNA extraction and gene detection using the polymerase chain reaction. The resistance rate to ceftazidime was 100%, cefepime 73.3%, piperacillin-tazobact...
Source: Journal of Water and Health - April 28, 2024 Category: Environmental Health Authors: Vin ícius Monteagudo de Barros Bruna Seixas da Rocha Jaqueline Rhoden Jana ína Franciele Stein Simone Ulrich Picoli Caroline Rigotto Source Type: research
Sulfonamide bioisosteres of niclosamide enhance antibacterial activity of colistin and bacitracin
ChemMedChem. 2024 Apr 28:e202400175. doi: 10.1002/cmdc.202400175. Online ahead of print.ABSTRACTMulticomponent therapy combining antibiotics with enhancer molecules known as adjuvants is an emerging strategy to combat antimicrobial resistance. Niclosamide is a clinically relevant anthelmintic drug with potential to be repurposed for its inherent antibacterial activity against Gram-positive bacteria and its ability to potentiate the antibacterial activity of colistin against susceptible and resistant Gram-negative bacteria. Herein, sulfonamide analogs of niclosamide were prepared and found to enhance colistin activity again...
Source: ChemMedChem - April 28, 2024 Category: Chemistry Authors: Liam Berry Danyel Ramirez Ronald Domalaon Frank Schweizer Source Type: research
Detection of metallo-beta-lactamase-producing genes < em > bla < /em > < sub > SPM < /sub > and < em > bla < /em > < sub > NDM < /sub > in < em > Pseudomonas aeruginosa < /em > isolated from wastewater in Southern Brazil
The objective of this study was to investigate genes encoding metallo-beta-lactamases (MBLs), which confer resistance to carbapenems, in wastewater. Fifteen isolates of P. aeruginosa were collected for five months from samples obtained from a municipal wastewater treatment plant in Rio Grande do Sul. These isolates were subjected to disk diffusion testing using 10 different antimicrobials. Phenotypic enzymatic tests for MBLs were conducted, and positive isolates underwent DNA extraction and gene detection using the polymerase chain reaction. The resistance rate to ceftazidime was 100%, cefepime 73.3%, piperacillin-tazobact...
Source: Journal of Water and Health - April 28, 2024 Category: Environmental Health Authors: Vin ícius Monteagudo de Barros Bruna Seixas da Rocha Jaqueline Rhoden Jana ína Franciele Stein Simone Ulrich Picoli Caroline Rigotto Source Type: research
Prevalence of antimicrobial resistance, biofilm formation, efflux pump activity, and virulence capabilities in multi-drug-resistant < em > Klebsiella pneumoniae < /em > isolated from freshwater fish farms
J Water Health. 2024 Apr;22(4):721-734. doi: 10.2166/wh.2024.382. Epub 2024 Apr 2.ABSTRACTThe present study aimed to determine the antibiotic resistance, underlying mechanisms, antibiotic residues, and virulence genes involved in 32 multi-drug-resistant Klebsiella pneumoniae isolates from freshwater fishes in Andhra Pradesh, India. Antibiogram studies revealed that all isolates were multi-drug-resistant, harbored tetA (96.8%), tetC (59.3%), tetD (71.9%), nfsA (59.3%), nfsB (53.1%), sul2 (68.7%), qnrC (43.7%), qnrD (50%), blaSHV (75%), blaTEM (68.7%), and blaCTX-M (93.7%) genes. Multiple antibiotic resistance index was calc...
Source: Journal of Water and Health - April 28, 2024 Category: Environmental Health Authors: Kummari Suresh Devika Pillai Source Type: research
Sulfonamide bioisosteres of niclosamide enhance antibacterial activity of colistin and bacitracin
ChemMedChem. 2024 Apr 28:e202400175. doi: 10.1002/cmdc.202400175. Online ahead of print.ABSTRACTMulticomponent therapy combining antibiotics with enhancer molecules known as adjuvants is an emerging strategy to combat antimicrobial resistance. Niclosamide is a clinically relevant anthelmintic drug with potential to be repurposed for its inherent antibacterial activity against Gram-positive bacteria and its ability to potentiate the antibacterial activity of colistin against susceptible and resistant Gram-negative bacteria. Herein, sulfonamide analogs of niclosamide were prepared and found to enhance colistin activity again...
Source: ChemMedChem - April 28, 2024 Category: Chemistry Authors: Liam Berry Danyel Ramirez Ronald Domalaon Frank Schweizer Source Type: research
Prevalence of antimicrobial resistance, biofilm formation, efflux pump activity, and virulence capabilities in multi-drug-resistant < em > Klebsiella pneumoniae < /em > isolated from freshwater fish farms
J Water Health. 2024 Apr;22(4):721-734. doi: 10.2166/wh.2024.382. Epub 2024 Apr 2.ABSTRACTThe present study aimed to determine the antibiotic resistance, underlying mechanisms, antibiotic residues, and virulence genes involved in 32 multi-drug-resistant Klebsiella pneumoniae isolates from freshwater fishes in Andhra Pradesh, India. Antibiogram studies revealed that all isolates were multi-drug-resistant, harbored tetA (96.8%), tetC (59.3%), tetD (71.9%), nfsA (59.3%), nfsB (53.1%), sul2 (68.7%), qnrC (43.7%), qnrD (50%), blaSHV (75%), blaTEM (68.7%), and blaCTX-M (93.7%) genes. Multiple antibiotic resistance index was calc...
Source: Journal of Water and Health - April 28, 2024 Category: Environmental Health Authors: Kummari Suresh Devika Pillai Source Type: research
Detection of metallo-beta-lactamase-producing genes < em > bla < /em > < sub > SPM < /sub > and < em > bla < /em > < sub > NDM < /sub > in < em > Pseudomonas aeruginosa < /em > isolated from wastewater in Southern Brazil
The objective of this study was to investigate genes encoding metallo-beta-lactamases (MBLs), which confer resistance to carbapenems, in wastewater. Fifteen isolates of P. aeruginosa were collected for five months from samples obtained from a municipal wastewater treatment plant in Rio Grande do Sul. These isolates were subjected to disk diffusion testing using 10 different antimicrobials. Phenotypic enzymatic tests for MBLs were conducted, and positive isolates underwent DNA extraction and gene detection using the polymerase chain reaction. The resistance rate to ceftazidime was 100%, cefepime 73.3%, piperacillin-tazobact...
Source: Journal of Water and Health - April 28, 2024 Category: Environmental Health Authors: Vin ícius Monteagudo de Barros Bruna Seixas da Rocha Jaqueline Rhoden Jana ína Franciele Stein Simone Ulrich Picoli Caroline Rigotto Source Type: research
Prevalence of antimicrobial resistance, biofilm formation, efflux pump activity, and virulence capabilities in multi-drug-resistant < em > Klebsiella pneumoniae < /em > isolated from freshwater fish farms
J Water Health. 2024 Apr;22(4):721-734. doi: 10.2166/wh.2024.382. Epub 2024 Apr 2.ABSTRACTThe present study aimed to determine the antibiotic resistance, underlying mechanisms, antibiotic residues, and virulence genes involved in 32 multi-drug-resistant Klebsiella pneumoniae isolates from freshwater fishes in Andhra Pradesh, India. Antibiogram studies revealed that all isolates were multi-drug-resistant, harbored tetA (96.8%), tetC (59.3%), tetD (71.9%), nfsA (59.3%), nfsB (53.1%), sul2 (68.7%), qnrC (43.7%), qnrD (50%), blaSHV (75%), blaTEM (68.7%), and blaCTX-M (93.7%) genes. Multiple antibiotic resistance index was calc...
Source: Journal of Water and Health - April 28, 2024 Category: Environmental Health Authors: Kummari Suresh Devika Pillai Source Type: research
Detection of metallo-beta-lactamase-producing genes < em > bla < /em > < sub > SPM < /sub > and < em > bla < /em > < sub > NDM < /sub > in < em > Pseudomonas aeruginosa < /em > isolated from wastewater in Southern Brazil
The objective of this study was to investigate genes encoding metallo-beta-lactamases (MBLs), which confer resistance to carbapenems, in wastewater. Fifteen isolates of P. aeruginosa were collected for five months from samples obtained from a municipal wastewater treatment plant in Rio Grande do Sul. These isolates were subjected to disk diffusion testing using 10 different antimicrobials. Phenotypic enzymatic tests for MBLs were conducted, and positive isolates underwent DNA extraction and gene detection using the polymerase chain reaction. The resistance rate to ceftazidime was 100%, cefepime 73.3%, piperacillin-tazobact...
Source: Journal of Water and Health - April 28, 2024 Category: Environmental Health Authors: Vin ícius Monteagudo de Barros Bruna Seixas da Rocha Jaqueline Rhoden Jana ína Franciele Stein Simone Ulrich Picoli Caroline Rigotto Source Type: research
Detection of Virulence Genes in Pseudomonas aeruginosa Isolates by Polymerase Chain Reaction and Investigation of High-Risk Clones by Matrix Assisted Laser Desorption/ Ionization Time-of-Flight Mass Spectrometer Method
Mikrobiyol Bul. 2024 Apr;58(2):135-147. doi: 10.5578/mb.202498112.ABSTRACTPseudomonas aeruginosa is a non-fermentative gram-negative bacillus. Many virulence factors play a role in the pathogenesis of P.aeruginosa. The aim of this study was to early detection of ST111, ST175, ST235, ST253, ST395 which are named high-risk clones with increased epidemic potential due to multidrug resistance in P.aeruginosa isolates by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) method and to evaluate the relationship between high-risk clones and the presence of P.aeruginosa virulence factors an...
Source: Mikrobiyoloji Bulteni - April 27, 2024 Category: Microbiology Authors: G ülşah Karacan Temür Yeliz Tanriverdi Çayci Asuman Birinci Source Type: research
Dissemination, virulence characteristic, antibiotic resistance determinants of emerging linezolid and vancomycin-resistant Enterococcus spp. in fish and crustacean
In conclusion, the emergence of linezolid-resistant -vancomycin-resistant enterococci recovered from Egyptian fish and shrimp, suggests that fish and seafood might participate a fundamental part in the emergence of antimicrobial resistance among humans.PMID:38677237 | DOI:10.1016/j.ijfoodmicro.2024.110711 (Source: International Journal of Food Microbiology)
Source: International Journal of Food Microbiology - April 27, 2024 Category: Food Science Authors: Sherief M Abdel-Raheem Sherin M Khodier Faisal Almathen Al-Shaimaa T Hanafy Sarah M Abbas Salah Abdulaziz Al-Shami Saad Ibrahim Al-Sultan Ahmed Alfifi Reham M El-Tarabili Source Type: research
Detection of Virulence Genes in Pseudomonas aeruginosa Isolates by Polymerase Chain Reaction and Investigation of High-Risk Clones by Matrix Assisted Laser Desorption/ Ionization Time-of-Flight Mass Spectrometer Method
Mikrobiyol Bul. 2024 Apr;58(2):135-147. doi: 10.5578/mb.202498112.ABSTRACTPseudomonas aeruginosa is a non-fermentative gram-negative bacillus. Many virulence factors play a role in the pathogenesis of P.aeruginosa. The aim of this study was to early detection of ST111, ST175, ST235, ST253, ST395 which are named high-risk clones with increased epidemic potential due to multidrug resistance in P.aeruginosa isolates by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) method and to evaluate the relationship between high-risk clones and the presence of P.aeruginosa virulence factors an...
Source: Mikrobiyoloji Bulteni - April 27, 2024 Category: Microbiology Authors: G ülşah Karacan Temür Yeliz Tanriverdi Çayci Asuman Birinci Source Type: research
