Neuroinductive properties of mGDNF depend on the producer, < i > E < /i > . < i > Coli < /i > or human cells

by Dzhirgala V. Shamadykova, Dmitry Y. Panteleev, Nadezhda N. Kust, Ekaterina A. Savchenko, Ekaterina Y. Rybalkina, Alexander V. Revishchin, Galina V. Pavlova The glial cell line‐derived neurotrophic factor (GDNF) is involved in the survival of dopaminergic neurons. Besides, GDNF can also induce axonal growth and creation of new functional synapses. GDNF potential is promising for translation to treat diseases associated with neuronal death: neurodege nerative disorders, ischemic stroke, and cerebral or spinal cord damages. Unproductive clinical trials of GDNF for Parkinson’s disease treatment have induced to study this failure. A reason could be due to irrelevant producer cells that cannot perform the required post-translational modifications. The biological activity of recombinant mGDNF produced byE.coli have been compared with mGDNF produced by human cells HEK293. mGDNF variants were tested with PC12 cells, rat embryonic spinal ganglion cells, and SH-SY5Y human neuroblastoma cellsin vitro as well as with a mouse model of the Parkinson ’s diseasein vivo. Bothin vitro andin vivo the best neuro-inductive ability belongs to mGDNF produced by HEK293 cells. Keywords: GDNF, neural differentiation, bacterial and mammalian expression systems, cell cultures, model of Parkinson ’s disease.
Source: PLoS One - Category: Biomedical Science Authors: Source Type: research