Molecular testing for H. pylori clarithromycin and quinolone resistance: a prospective Chinese study

AbstractIn China, there is a high prevalence of antibiotic-resistantHelicobacter pylori infections in the population. The aim of the study was to assess a new ARMS-PCR test for detection ofH. pylori clarithromycin resistance (CR) and quinolone resistance (QR) mutations and evaluate the spectrum of antibiotic resistance in patients from three Chinese provinces. Sanger sequencing and multiplex ARMS-PCR were used to detectH. pylori CR and QR bacteria in gastric biopsy samples. Among the 1,182 patients enrolled with gastritis, 643 (54.4%) were positive forH. pylori. Of these, 371 (57.7%) had antibiotic-resistant strains, comprising 236 (63.6%) with a single drug antibiotic-resistant strain and 135 (36.4%) with multiple drug-resistant strains. Following Sanger sequencing analysis of23S rRNA andgyrA gene for mutations (antibiotic resistance markers), rates of CR, QR, and multidrug resistance (CR and QR) were 19.9, 12.0, and 25.8%, respectively. The23S rRNA CR mutation A2143G (286, 96.9%) and thegyrA QR mutations C261A (85, 31.5%) and G271A (71, 26.3%) were common. Benchmarking against Sanger sequencing results, multiplex ARMS-PCR test had a high diagnostic sensitivity and specificity for detection of CR (96 and 93%), QR (95 and 92%) and multidrug resistance (95 and 95%). Based on our findings, the high incidence of single and multiple antibiotic resistance requires the routine checking of antibiotic resistance in all patients with suspectedH. pylori infections. Multiplex ARMS-PCR i...
Source: European Journal of Clinical Microbiology and Infectious Diseases - Category: Microbiology Source Type: research