Throat microbial community structure and functional changes in post-surgery laryngeal carcinoma patients.

Throat microbial community structure and functional changes in post-surgery laryngeal carcinoma patients. Appl Environ Microbiol. 2020 Oct 02;: Authors: Hsueh CY, Gong H, Cong N, Sun J, Lau HC, Guo Y, Huang Q, Yuan X, Zhang M, Tao L, Zhou L Abstract Microbial community structure and its shift after laryngectomy in laryngeal squamous cell carcinoma (LSCC) patients were investigated. Thirty swab samples of LSCC prior to laryngectomy (SLC), 18 samples after 1-weeks laryngectomy (SLCA1w), and 30 samples after 24-week laryngectomy (SLCA24w) from 30 LSCC patients were sourced. Microbial diversity was profiled through sequencing the V3-4 variable region of the 16S ribosomal ribonucleic acid (rRNA) gene. Quantitative real-time polymerase chain reaction (qPCR) was used to validate the V3-4 region of 16S rRNA sequence data. The community structure and function of throat microbiota were assessed by PICRUSt analysis. Both Alpha and Beta diversity results showed significant differences in the throat microbiota of LSCC patients before and after laryngectomy (P < 0.05). Drinking index of SLC group was positively associated with the genus abundance of Prevotella (P < 0.05). SLCA1w group had lower abundance of Fusobacterium, Leptotrichia, Lachnoanaerobaculum and Veillonella compared to the SLC group (P < 0.05). SLCA24w group had higher abundance of Streptococcus and Leptotrichia, as well as the lower abundance of Fusobacterium and Alloprevot...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research