Evaluation of different sterilization methods for decellularized kidney tissue.

Evaluation of different sterilization methods for decellularized kidney tissue. Tissue Cell. 2020 Oct;66:101396 Authors: Moradi L, Mohammadi Jobania B, Jafarnezhad-Ansariha F, Ghorbani F, Esmaeil-Pour R, Majidi Zolbina M, Kajbafzadeh AM Abstract The main goal of this study was to assess the effect of different sterilization treatment for sterilization of decellularized kidney tissue. Rabbit kidneys were decellularized by the perfusion-based method using sodium dodecyl sulfate (SDS) and Triton X-100. Then, decellularized kidney slices were prepared and sterilized by an antibiotic cocktail, PAA (0.5 %, 1% and 1.5 %), 5KG γ-irradiation and 320-480 nm UV-irradiation. Histological evaluations, DNA quantification assay, MTT assay, scanning electron microscopy (SEM), mechanical test and bacterial and fungal culture tests were performed to determine the quality of decellularization and sterilization processes. The kidney slices were seeded by adipose-derived mesenchymal stem cells (ASCs) to assess the cell adhesion capability after treatment. The results of the current study indicated that PAA 0.5 % was the most efficient method to completely decontaminate rabbit decellularized kidney tissue while preserving the mechanical properties and main components of the matrix which are necessary for cell-matrix interaction and cell adhesion. The 5KG γ-irradiation was determined to be the most destructive sterilization method, with reduced the mec...
Source: Tissue and Cell - Category: Cytology Authors: Tags: Tissue Cell Source Type: research