Enrichment and Transcriptional Characterization of Stem Cells Isolated from Human Glioblastoma Cell Lines.
Enrichment and Transcriptional Characterization of Stem Cells Isolated from Human Glioblastoma Cell Lines.
Methods Mol Biol. 2021;2174:19-29
Authors: Piña-Medina AG, Hernández-Vega AM, Díaz NF, Mancilla-Herrera I, Camacho-Arroyo I
Abstract
Glioblastomas (GBM) are the most frequent and aggressive brain tumors due to their recurrence and resistance to current therapies. These characteristics are associated with the presence of glioma stem cells (GSCs), mainly identified by the detection of the membrane antigens CD133 and CD15. The main source of GSCs has been biopsies of tumors. However, alternatives are sought from cell lines because more homogeneous populations can be obtained with high yields. This chapter describes a method for the enrichment and characterization of GSCs from cell lines derived from human GBM by selective culture with serum-free neural stem cell medium and growth factors. The technique offers alternatives for the enrichment and characterization of GSCs, that could contribute to a better understanding of the biology of GBMs.
PMID: 32813242 [PubMed - in process]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Piña-Medina AG, Hernández-Vega AM, Díaz NF, Mancilla-Herrera I, Camacho-Arroyo I Tags: Methods Mol Biol Source Type: research
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