Prevalence of Helicobacter pylori babA , oipA , sabA , and homB genes in isolates from Chinese patients with different gastroduodenal diseases

This study aimed to investigate the correlation between the four OMP genes (babA,oipA,sabA, andhomB) and gastroduodenal diseases. One hundred and seventy-sevenH. pylori strains were isolated from Chinese patients with different gastroduodenal diseases (49 chronic gastritis, 19 gastric ulcer, 33 gastric cancer, and 76 duodenal ulcer), 94 of which contained pathological information (41 superficial gastritis, 24 intestinal hyperplasia, and 29 gastric adenocarcinoma). The full-length amplification ofbabA,oipA,sabA,and homB genes was acquired and sequenced. Then, the genetic polymorphism was analyzed to compare with the reference strains from the GenBank database. Functional status and cluster analysis were also performed to evaluate the impact of genetic polymorphism on disease outcome. The prevalence ofbabA,oipA,sabA, andhomB genes were 91.5%, 100%, 94.0%, and 95.5%, respectively. The four OMP genes were characterized by genetic polymorphism and in the status of positive selection (Ka/Ks>  1). The proportion of strains with functional status on foroipA andsabA gene was 100% and 76.2%, respectively. The sequences of four OMP genes were mainly clustered together with the East Asian references. The four OMP genes were not different in patients with gastroduodenal diseases and pathologic changes (P  >  0.05).H. pylori babA,oipA,sabA, andhomB genes were common in the Chinese populations, but did not seem to be involved in the development of gastroduodenal diseases.
Source: Medical Microbiology and Immunology - Category: Microbiology Source Type: research