GH and GHR gene cloning, expression and their associations with growth-related traits of the barbel chub (Squaliobarbus curriculus)
Publication date: Available online 22 February 2020Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular BiologyAuthor(s): Jing'an Wang, Dongfang Li, Xin Zhao, Tong Sun, Shengzhen Jin, Hongquan Wang, Tiaoyi Xiao, Yaoguo LiAbstractGrowth hormone (ScGH) and growth hormone receptor (ScGHR) genes from the barbel chub (Squaliobarbus curriculus), in addition to their cDNAs, were cloned. The associations between their mRNA expression levels and growth-related traits were analysed, and the differences in the levels of expression of growth regulation-related genes between the largest and smallest individuals were compared. The full-length 1182-bp cDNA of ScGH contained a 633-bp open reading frame (ORF), and the length of the gene had 2492 bp. The full-length 2825-bp cDNA of ScGHRa contained a 1818-bp ORF, and the gene had 6970 bp. The full-length 2822-bp cDNA of ScGHRb contained a 1737-bp ORF, and the gene had 8149 bp. Quantitative real-time PCR revealed that ScGH was only expressed in the pituitary. ScGHRa was expressed predominantly in muscle, and the expression level of ScGHRb was the highest in the liver. The ScGHRa mRNA levels in the muscle were significantly negatively correlated with the caudal peduncle length. However, no correlation between growth-related traits and ScGH and ScGHRb expression levels were found. Pituitary ScGH, liver GHRb and liver insulin-like growth factor I (igf-1) expression levels were significantly higher in the largest...
Publication date: June 2020Source: Journal of Functional Foods, Volume 69Author(s): Linlin Wang, Shi Cen, Gang Wang, Yuan-kun Lee, Jianxin Zhao, Hao Zhang, Wei Chen
Publication date: June 2020Source: Journal of Functional Foods, Volume 69Author(s): Mingsi Xie, Jie Liu, Ziyuan Wang, Baoguo Sun, Jing Wang
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