Role of PfGCN5 in nutrient sensing and transcriptional regulation in Plasmodium falciparum.

In this study, we show upregulation of PfGCN5 upon nutrient stress condition. With the help of chromatin immunoprecipitation coupled high-throughput sequencing (ChIP-seq) and transcriptomic (RNA-sequencing) analyses, we show that PfGCN5 is associated with the genes that are important for the maintenance of parasite cellular homeostasis upon nutrient stress condition. Furthermore, we identified various metabolic enzymes as interacting partners of PfGCN5 by immunoprecipitation coupled with mass spectroscopy, possibly acting as a sensor of nutrient conditions in the environment. We also demonstrated that PfGCN5 interacts and acetylates PfGAPDH in vitro. Collectively, our data provides important insights into transcriptional deregulation upon nutrient stress condition and elucidate the role of PfGCN5 during nutrient stress condition. PMID: 31965989 [PubMed - in process]
Source: Journal of Biosciences - Category: Biomedical Science Authors: Tags: J Biosci Source Type: research