LncRNA TTN-AS1 regulates osteosarcoma cell apoptosis and drug resistance via the miR-134-5p/MBTD1 axis.
CONCLUSION: In osteosarcoma, lncRNA TTN-AS1 promoted the expression of MBTD1 by targeting miR-134-5p and regulated cell growth, apoptosis and drug resistance.
METHODS: The expression characteristics of genes in osteosarcoma patients were analysed using bioinformatics. Plasmid transfection technology was applied to silence or overexpress lncRNA TTN-AS1, miR-134-5p and MBTD1. Western blotting and quantitative polymerase chain reaction (qPCR) were used to detect protein and RNA, respectively. A cell counting kit 8 (CCK-8) and flow cytometry were used to detect cell viability and apoptosis. The effects of lncRNA TTN-AS1 and MBTD1 on osteosarcoma in vivo were studied by using a tumour burden assay.
PMID: 31600142 [PubMed - as supplied by publisher]
Source: Aging - Category: Biomedical Science Authors: Fu D, Lu C, Qu X, Li P, Chen K, Shan L, Zhu X Tags: Aging (Albany NY) Source Type: research
More News: Biomedical Science | Bone Cancers | Brain | Brain Cancers | Brain Tumor | Cancer | Cancer & Oncology | Genetics | Neurology | Osteosarcoma