In Vitro Models, Standards, and Experimental Methods for Tobacco Products.

In Vitro Models, Standards, and Experimental Methods for Tobacco Products. Adv Dent Res. 2019 Oct;30(1):16-21 Authors: Aghaloo T, Kim JJ, Gordon T, Behrsing HP Abstract Traditional tobacco products have well-known systemic and local oral effects, including inflammation, vasoconstriction, delayed wound healing, and increased severity of periodontal disease. Specifically in the oral cavity and the lung, cigarette smoking produces cancer, increased infectivity, acute and chronic inflammation, changes in gene expression in epithelial lining cells, and microbiome changes. In recent years, cigarette smoking has greatly decreased in the United States, but the use of new tobacco products has gained tremendous popularity. Without significant knowledge of the oral sequelae of products such as electronic cigarettes, researchers must evaluate current in vitro and in vivo methods to study these agents, as well as develop new tools to adequately study their effects. Some in vitro testing has been performed for electronic cigarettes, including toxicologic models and assays, but these mostly study the effect on the respiratory tract. Recently, direct exposure of the aerosol to in vitro 3-dimensional tissue constructs has been performed, demonstrating changes in cell viability and inflammatory cytokines. For in vivo studies, a universal e-cigarette testing machine or standard vaping regime is needed. A standard research electronic cigarette has recently been developed by...
Source: Adv Dent Res - Category: Dentistry Authors: Tags: Adv Dent Res Source Type: research

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Publication date: January 2020Source: Biomedical Signal Processing and Control, Volume 55Author(s): Archana Nawandhar, Navin Kumar, Veena R, Lakshmi YamujalaAbstractSquamous cell carcinoma (SCC) of oral cavity is the most common among oral cancer patients. In this paper, we have developed machine learning based automatic oral squamous cell carcinoma (OSCC) classifier named as Stratified Squamous Epithelial Biopsy Image Classifier (SSE-BIC) to categorize H&E-stained microscopic images of squamous epithelial layer in four different classes: normal, well-differentiated, moderately-differentiated and poorly-differentiated. Fiv...
Source: Biomedical Signal Processing and Control - Category: Biomedical Science Source Type: research
Publication date: Available online 19 September 2019Source: Seminars in Cancer BiologyAuthor(s): Annie Wai Yeeng Chai, Kue Peng Lim, Sok Ching CheongAbstractOral squamous cell carcinomas (OSCC) are a heterogeneous group of cancers arising from the mucosal lining of the oral cavity. A majority of these cancers are associated with lifestyle risk habits including smoking, excessive alcohol consumption and betel quid chewing. Cetuximab, targeting the epidermal growth factor receptor was approved for the treatment of OSCC in 2006, and remains the only molecular targeted therapy available for OSCC. Here, we reviewed the current ...
Source: Seminars in Cancer Biology - Category: Cancer & Oncology Source Type: research
Authors: Rai K H, Ahmed J Abstract Background: Epithelial cells typically express E-cadherin where as N-cadherin expressed by mesenchymal cells. The epithelial to mesenchymal transition (EMT) is a process by which epithelial cells lose their cell polarity and cell-cell adhesion, and gain migratory and invasive properties to become mesenchymal cells. EMT is typical for carcinoma cells during tumor progression and correlate with the local invasiveness and metastatic potential of the tumor. Oral squamous cell carcinoma is a malignant neoplasm arising from the mucosal epithelium of the oral cavity. It can be classified...
Source: Asian Pacific Journal of Cancer Prevention - Category: Cancer & Oncology Tags: Asian Pac J Cancer Prev Source Type: research
Conclusion: CD44, a cell membrane marker could help in cell adhesion and cell-cell interactions. Loss of CD44 expression enhances the binding of the growth factors with their principle receptors that enhances the cellular proliferation. It can be used as a prognostic marker for identifying the rate of malignant transformation in these disorders.
Source: Journal of Oral and Maxillofacial Pathology - Category: ENT & OMF Authors: Source Type: research
Conclusion: On evaluation of the survey, we found that 85% of the surveyees are on agreement that PPOELs should be registered in a standard format and should be included in the list of recognizable diseases. 89.2% and 87.8% of the respondents would like to be part of an active body for early detection and diagnosis and for Cancer screening in our country respectively. In conclusion, the oral pathology fraternity is eager to work for and tackle these PPOELs head on, provided the right opportunities and training are meted out to them.
Source: Journal of Oral and Maxillofacial Pathology - Category: ENT & OMF Authors: Source Type: research
Conclusion: MN scoring on the epithelial cells of buccal mucosa can be used as a biomarker in screening procedures for HIV patients.
Source: Journal of Oral and Maxillofacial Pathology - Category: ENT & OMF Authors: Source Type: research
Conclusion: We recommend that methylene blue staining is a useful diagnostic adjunct in a large, community-based oral cancer screening program for high-risk individuals.
Source: Journal of Oral and Maxillofacial Pathology - Category: ENT & OMF Authors: Source Type: research
Abstract Tongue squamous cell carcinoma (TSCC), the most common epithelial cancer identified in the oral cavity, has become one of the most common malignancies across the developing countries. Increasing evidence indicates that long non-coding RNAs (lncRNAs) serve as important regulators in cancer biology. The focally amplified long non-coding RNA in epithelial cancer (FALEC) was found downregulated in the tissues of tongue squamous cell carcinoma (TSCC) and was predicted to present a good prognosis by bioinformatics analysis. Experiments indicated that FALEC knockdown significantly increased the proliferation and...
Source: Aging - Category: Biomedical Science Authors: Tags: Aging (Albany NY) Source Type: research
Publication date: July 2019Source: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology, Volume 128, Issue 1Author(s): Dr. Vimi Mutalik, Dr. Kristin McNamara, Dr. John Draper, Dr. John KalmarObjectiveExpression of the protein marker p 16INK4a is used as a surrogate for human papillomavirus (HPV) infection in biopsies of oral and tonsillar mucosa. While HPV infection accounts for
Source: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology - Category: ENT & OMF Source Type: research
ConclusionEZH2 expression in AEP is more similar to malignant processes than benign lesions. The pattern of basal cell layer expression of EZH2 could be a potential prognostic indicator of malignant transformation risk in oral AEP lesions. A subsequent study by our group to assess EZH2 expression with respect to clinical outcome in AEP lesions is ongoing.
Source: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology - Category: ENT & OMF Source Type: research
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