Effects of dietary anthocyanin on innate immune parameters, gene expression responses, and ammonia resistance of Nile tilapia (Oreochromis niloticus)

Publication date: October 2019Source: Fish & Shellfish Immunology, Volume 93Author(s): Ebru YilmazAbstractThe present study investigated the effects of dietary anthocyanin on the growth performance, haematological, non-specific immune, and spleen gene expression responses of Nile tilapia, Oreochromis niloticus. Five experimental groups of fish with mean weights of 8.24 ± 0.64 g were used in the study; four of these were fed with diets incorporating anthocyanin (20 mg kg -1, 40 mg kg−1, 80 mg kg−1 and 160 mg kg−1), while the fifth was a control group without dietary anthocyanin. Growth performance and haematological parameters of tilapia were not affected by anthocyanin-supplemented diets (p > 0.05). Dietary anthocyanin significantly increased respiratory burst activity, phagocytic activity, phagocytic index, lysozyme activity, myeloperoxidase activity, serum total superoxide dismutase (T.SOD) activity, and serum catalase (CAT) activity (p < 0.05). The total immunoglobulin level was highest in the 80 mg kg−1 group compared with the other groups (p < 0.05). In addition, with the anthocyanin-containing diets, the gene levels of interleukin 1, beta (IL-1β), interleukin 8 (IL-8), tumor necrosis factor (TNF-α), heat shock protein 70 (HSP70), and interferon gamma (IFN-γ) were increased in the fish spleen, and the gene levels of CAT, GPx, and SOD were also increased in fish liver (p < 0.05). At the end of the experimen...
Source: Fish and Shellfish Immunology - Category: Biology Source Type: research