Integrated transcriptomic and functional immunological approach for assessing the invasiveness of bivalve alien species
Publication date: August 2019Source: Fish & Shellfish Immunology, Volume 91Author(s): A. Romero, R. Aranguren, R. Moreira, B. Novoa, A. Figueras (Source: Fish and Shellfish Immunology)
Source: Fish and Shellfish Immunology - June 18, 2019 Category: Biology Source Type: research

Transcriptomic analysis of clam extra pallial fluids reveals immunity and cytoskeleton alterations in the first week of brown ring disease development
Publication date: August 2019Source: Fish & Shellfish Immunology, Volume 91Author(s): Alexandra Rahmani, Erwan Corre, Gaëlle Richard, Adeline Bidault, Louisi Oliveira, Fabiano Thompson, Christine Paillard, Vianney Pichereau (Source: Fish and Shellfish Immunology)
Source: Fish and Shellfish Immunology - June 18, 2019 Category: Biology Source Type: research

Vibrio aestuarianus virulent traits : Insights from in vitro interaction with oyster hemocytes
Publication date: August 2019Source: Fish & Shellfish Immunology, Volume 91Author(s): A. Mesnil, D. Tourbiez, C. Garcia, C. Lambert, M.A. Travers (Source: Fish and Shellfish Immunology)
Source: Fish and Shellfish Immunology - June 18, 2019 Category: Biology Source Type: research

Immune response of common carp to presporogonic development of myxozoan Sphaerospora molnari
Publication date: August 2019Source: Fish & Shellfish Immunology, Volume 91Author(s): T. Korytář, G.F. Wiegertjes, E. Zusková, A. Tomanová, M. Lisnerová, S. Patra, V. Sieranski, R. Šíma, A. Born-Torrijos, A.S. Wentzel, S. Blasco-Monleon, C. Yanes-Roca, T. Policar, A.S. Holzer (Source: Fish and Shellfish Immunology)
Source: Fish and Shellfish Immunology - June 18, 2019 Category: Biology Source Type: research

From gross morphology to gill transcriptome in farmed Atlantic salmon (Salmo salar): Lessons from multi-site sampling
Publication date: August 2019Source: Fish & Shellfish Immunology, Volume 91Author(s): E. Król, A. Douglas, P. Noguera, V. Valdenegro, K. Gajardo, R. Bickerdike, S.A.M. Martin (Source: Fish and Shellfish Immunology)
Source: Fish and Shellfish Immunology - June 18, 2019 Category: Biology Source Type: research

A proteomic study of resistance to brown ring disease in the manila clam, Ruditapes philippinarum
Publication date: August 2019Source: Fish & Shellfish Immunology, Volume 91Author(s): M. Smits, S. Artigaud, B. Bernay, A. Bidault, L. Bargelloni, C. Paillard (Source: Fish and Shellfish Immunology)
Source: Fish and Shellfish Immunology - June 18, 2019 Category: Biology Source Type: research

Insights into the microbiota of farmed and wild Mytilus SP: Is there a link between bacteria communities and host susceptibility?
Publication date: August 2019Source: Fish & Shellfish Immunology, Volume 91Author(s): Yosra Ben Cheikh, Marie Agnès Travers, Frank Le Foll, Nathalie Giusti (Source: Fish and Shellfish Immunology)
Source: Fish and Shellfish Immunology - June 18, 2019 Category: Biology Source Type: research

Gene encoding enzymes in the urea cycle and polyamine synthesis are modulated during an inflammatory response in rainbow trout (Oncorhynchus mykiss)
Publication date: August 2019Source: Fish & Shellfish Immunology, Volume 91Author(s): T. Clark, J. Tinsley, D.J.Macqueen, S.A.M. Martin (Source: Fish and Shellfish Immunology)
Source: Fish and Shellfish Immunology - June 18, 2019 Category: Biology Source Type: research

Effect of different stressors on the expression of glucocorticoid receptor 1 (GR1) and GR2 and their implications in the transcriptional immune response in mucosal surfaces
Publication date: August 2019Source: Fish & Shellfish Immunology, Volume 91Author(s): Eva Vallejos-Vidal, Beatriz Sanz Milián, Sara Urquizu, Aleix Castellvi, Irene Brandts Busom, Mariana Teles, Juan Miguel Mancera, Lluis Tort, Felipe E. Reyes-López (Source: Fish and Shellfish Immunology)
Source: Fish and Shellfish Immunology - June 18, 2019 Category: Biology Source Type: research

Moritella viscosa in lumpfish (Cyclopterus lumpus) and Atlantic salmon (Salmo salar)
Publication date: August 2019Source: Fish & Shellfish Immunology, Volume 91Author(s): T. Einarsdottir, H. Sigurdardottir, E. Einarsdottir, T.S. Bjornsdottir (Source: Fish and Shellfish Immunology)
Source: Fish and Shellfish Immunology - June 18, 2019 Category: Biology Source Type: research

IFIT5 participates in the antiviral mechanisms of rainbow trout red blood cells
Publication date: August 2019Source: Fish & Shellfish Immunology, Volume 91Author(s): V. Chico, M. Salvador-Mira, I. Nombela, S. Puente-Marin, S. Ciordi, M.C. Mena, L. Perez, J. Coll, F. Guzman, J. Encinar, L. Mercado, M. Ortega-Villaizan (Source: Fish and Shellfish Immunology)
Source: Fish and Shellfish Immunology - June 18, 2019 Category: Biology Source Type: research

Analysis of immune gene expression during agd infection/reinfection of Atlantic salmon (Salmo salar)
Publication date: August 2019Source: Fish & Shellfish Immunology, Volume 91Author(s): Anna Harte, Stanko Skugor, Chris Hawes, Christopher Secombes (Source: Fish and Shellfish Immunology)
Source: Fish and Shellfish Immunology - June 18, 2019 Category: Biology Source Type: research

White spot syndrome virus infection in a crustacean
Publication date: August 2019Source: Fish & Shellfish Immunology, Volume 91Author(s): Hai-peng Liu, Chuang Meng, Dong-li Li, Ling-ke Liu (Source: Fish and Shellfish Immunology)
Source: Fish and Shellfish Immunology - June 18, 2019 Category: Biology Source Type: research

Prohibitin 2 is associated with WSSV infection by promoting stat translocation
Publication date: August 2019Source: Fish & Shellfish Immunology, Volume 91Author(s): Cao Xiao-Tong, Zhang Ying-Hao, Lan Jiang-Feng (Source: Fish and Shellfish Immunology)
Source: Fish and Shellfish Immunology - June 18, 2019 Category: Biology Source Type: research

Comparison of gene expression in post-smolt Atlantic salmon challenged by LF-89-like and EM-90-like Piscirickettsia salmonis isolates reveals differences in the immune response associated with pathogenicity
Publication date: August 2019Source: Fish & Shellfish Immunology, Volume 91Author(s): M. Rozas-Serri, A. Peña, G. Arriagada, L. Maldonado (Source: Fish and Shellfish Immunology)
Source: Fish and Shellfish Immunology - June 18, 2019 Category: Biology Source Type: research

The microbiome and transcriptome analysis of intestinal microbiota communities in larval and diseased grouper
Publication date: August 2019Source: Fish & Shellfish Immunology, Volume 91Author(s): Joan Tang Xiao Joe, Ming-Wei Lu (Source: Fish and Shellfish Immunology)
Source: Fish and Shellfish Immunology - June 18, 2019 Category: Biology Source Type: research

Identification of novel biomarkers asscociated with infection and chronic stress in Russian sturgeon
Publication date: August 2019Source: Fish & Shellfish Immunology, Volume 91Author(s): Mauricio Castellano, Valeria Silva-Álvarez, Marcio Aversa, Alicia Costábile, Ignacio Quartiani, Daniel Conijeski, Alejandro Perretta, Andrea Villarino, Ana M. Ferreira (Source: Fish and Shellfish Immunology)
Source: Fish and Shellfish Immunology - June 18, 2019 Category: Biology Source Type: research

A lectin with LDL receptor domain suppress the replication of WSSV
Publication date: August 2019Source: Fish & Shellfish Immunology, Volume 91Author(s): Li Tong, Zhang Ying-Hao, Lan Jiang-Feng (Source: Fish and Shellfish Immunology)
Source: Fish and Shellfish Immunology - June 18, 2019 Category: Biology Source Type: research

A comparative study on intestinal immune cells in two elasmobranchs species
Publication date: August 2019Source: Fish & Shellfish Immunology, Volume 91Author(s): B. Sayyaf Dezfuli, P. Merella, G. Bosi, M. Manera, L. Giari (Source: Fish and Shellfish Immunology)
Source: Fish and Shellfish Immunology - June 18, 2019 Category: Biology Source Type: research

Liza ramada innate immunity against intestinal microparasite, Myxobolus mugchelo (Myxozoa)
Publication date: August 2019Source: Fish & Shellfish Immunology, Volume 91Author(s): B. Sayyaf Dezfuli, G. Bosi, M. Lanzoni, F. Pironi, G. Castaldelli, L. Giari (Source: Fish and Shellfish Immunology)
Source: Fish and Shellfish Immunology - June 18, 2019 Category: Biology Source Type: research

Sponsors for ISFSI 2019
Publication date: August 2019Source: Fish & Shellfish Immunology, Volume 91Author(s): (Source: Fish and Shellfish Immunology)
Source: Fish and Shellfish Immunology - June 18, 2019 Category: Biology Source Type: research

Program for ISFSI 2019
Publication date: August 2019Source: Fish & Shellfish Immunology, Volume 91Author(s): (Source: Fish and Shellfish Immunology)
Source: Fish and Shellfish Immunology - June 18, 2019 Category: Biology Source Type: research

Adverstisment BIOASiS
Publication date: August 2019Source: Fish & Shellfish Immunology, Volume 91Author(s): (Source: Fish and Shellfish Immunology)
Source: Fish and Shellfish Immunology - June 18, 2019 Category: Biology Source Type: research

AMMR 2019 - Call for abstracts
Publication date: August 2019Source: Fish & Shellfish Immunology, Volume 91Author(s): (Source: Fish and Shellfish Immunology)
Source: Fish and Shellfish Immunology - June 18, 2019 Category: Biology Source Type: research

Differentially expressed genes of the shrimp Marsupenaeus japonicus in response to infection by white spot syndrome virus or Vibrio alginolyticus
Publication date: Available online 16 June 2019Source: Fish & Shellfish ImmunologyAuthor(s): Qingri Jin, Fei ZhuAbstractThis is the first study to use a next-generation high-throughput DNA sequencing technique, the Illumina Hiseq2000 method, to analyze the transcriptome from hemocytes of Marsupenaeus japonicus. A total of 80,929,652 Illumina reads, including 79,525,942 high quality reads were obtained in this study. From these, 40,231 unigenes with a mean length of 1557 bp were assembled using Trinity de novo software and 28,746 cDNA were matched in the NCBI database. Then we compared the transcriptome changes after wh...
Source: Fish and Shellfish Immunology - June 16, 2019 Category: Biology Source Type: research

Dietary supplementation with polypeptides improved growth performance, antibacterial immune and intestinal microbiota structure of Litopenaeus vannamei
Publication date: Available online 14 June 2019Source: Fish & Shellfish ImmunologyAuthor(s): Xu-zheng Liao, Shi-kang Hu, Bo Wang, Hai-peng Qin, Ji-chen Zhao, Zi-hao He, Xie-yan Chen, Yong-sheng Liu, Peng Qu, Cheng-bo Sun, Shuang ZhangAbstractAntibacterial peptides (AMPs) are expected to replace some or all of the antibiotics and become a new feed additive. However, the high production cost and unclear mechanism limited the application of AMPs. In this research, the effects of a commercial polypeptide (Polypeptide S100) whose main components are AMPs on the growth, antibacterial immune and intestinal microbial of Litope...
Source: Fish and Shellfish Immunology - June 15, 2019 Category: Biology Source Type: research

Differential proteomic profiles and characterizations between hyalinocytes and granulocytes in ivory shell Babylonia areolata
Publication date: Available online 15 June 2019Source: Fish & Shellfish ImmunologyAuthor(s): Guilan Di, Yanfei Li, Xianliang Zhao, Ning Wang, Jingqiang Fu, Min Li, Miaoqin Huang, Weiwei You, Xianghui Kong, Caihuan KeAbstractThe haemocytes of the ivory shell, Babylonia areolata are classified by morphologic observation into the following types: hyalinocytes (H) and granulocytes (G). Haemocytes comprise diverse cell types with morphological and functional heterogene and play indispensable roles in immunological homeostasis of invertebrates. In the present study, two types of haemocytes were morphologically identified and...
Source: Fish and Shellfish Immunology - June 15, 2019 Category: Biology Source Type: research

Functional characterization of three fish-specific interleukin-23 isoforms as regulators of Th17 signature cytokine expression in grass carp head kidney leukocytes
Publication date: Available online 13 June 2019Source: Fish & Shellfish ImmunologyAuthor(s): Licheng Yin, Jingqi Ren, Dan Wang, Shiyu Feng, Xinyang Qiu, Mengyuan Lv, Xinyan Wang, Hong ZhouAbstractMammalian Interleukin (IL)-23 is a heterodimeric cytokine with an IL-23-specific P19 subunit and a P40 subunit shared with IL-12, and plays a key role in the regulation of cell differentiation as well as inflammation. We previously demonstrated the existence of three soluble fish Interleukin (Il)-23 isoforms consist of a single P19 and one of three P40 isoforms (P40a/b/c) in grass carp. In the present study, three recombinant ...
Source: Fish and Shellfish Immunology - June 15, 2019 Category: Biology Source Type: research

Multiplex genome editing by natural transformation in Vibrio mimicus with potential application in attenuated vaccine development
This study provides a convenient method for the genetic manipulation of V. mimicus. It will facilitate the identification and characterization of V. mimicus virulence factors and eventually contribute to a better understanding of V. mimicus pathogenicity and development of attenuated vaccine. (Source: Fish and Shellfish Immunology)
Source: Fish and Shellfish Immunology - June 15, 2019 Category: Biology Source Type: research

Pathogenicity of non-O1/ O139 Vibrio cholerae and its induced immune response in Macrobrachium rosenbergii
Publication date: Available online 13 June 2019Source: Fish & Shellfish ImmunologyAuthor(s): Xiaojian Gao, Zhen Miao, Xixi Li, Nan Chen, Wenwen Gu, Xiaodan Liu, Hui Yang, Wanhong Wei, Xiaojun ZhangAbstractOutbreaks of mass mortalities occurred in Macrobrachium rosenbergii farms in Gaoyou county, Jiangsu Province of China. The bacterial isolates from M. rosenbergii exhibited the same phenotypic traits and biochemical characteristics, and were identified as non-O1/O139 Vibrio cholerae according to biochemical characteristics and molecular identification. In challenge test, M. rosenbergii infected with non-O1/O139 V. chol...
Source: Fish and Shellfish Immunology - June 15, 2019 Category: Biology Source Type: research

Overwinter mortality in yellow drum (Nibea albiflora): Insights from growth and immune responses to cold and starvation stress
Publication date: Available online 13 June 2019Source: Fish & Shellfish ImmunologyAuthor(s): Hongbin Song, Dongdong Xu, Lu Tian, Ruiyi Chen, Ligai Wang, Peng Tan, Qiaochu YouAbstractThe yellow drum (Nibea albiflora) is an economically important maricultured fish in China, but the aquaculture of this species has recently been limited by an increase in overwinter mortalities associated with cold and starvation stress due to global climate changes. To better understand the interaction between starvation and cold-stress-driven overwinter mortality, we investigated the effects of these stresses on the growth performance, li...
Source: Fish and Shellfish Immunology - June 15, 2019 Category: Biology Source Type: research

Identification and molecular characterization of peroxiredoxin 2 in grass carp (Ctenopharyngodon idella)
In this study, the molecular cloning and characterization of a Prx2 homologue (CiPrx2) were described from grass carp (Ctenopharyngodon idella). The full-length cDNA of CiPrx2 was 1163 bp containing 5ʹ-untranslated region (UTR) of 52 bp, a 3′-UTR of 517 bp with the putative polyadenylation consensus signal (AATAAA), an open reading frame (ORF) of 594 bp encoding polypeptides of 197 amino acids with a predicted molecular mass of 21.84 kDa and theoretical isoelectric point of 5.93. The analysis results of multiple sequence alignment and phylogenetic tree confirmed that CiPrx2 belong to the typical 2-Cys Prx subfamil...
Source: Fish and Shellfish Immunology - June 15, 2019 Category: Biology Source Type: research

Functional differentiation of three phosphatidylinositol 3-kinase catalytic subunit alpha (PIK3CA) in response to Vibrio anguillarum infection in turbot (Scophthalmus maximus)
Publication date: Available online 14 June 2019Source: Fish & Shellfish ImmunologyAuthor(s): Kai Zhang, Xiumei Liu, Miao Han, Yuxiang Liu, Xuangang Wang, Haiyang Yu, Jinxiang Liu, Quanqi ZhangAbstractPIK3CA has been extensively investigated from its molecular mechanism perspective and association with its mutations in different types of cancers. However, little has been reported regarding the pathological significance of PIK3CA expression in teleost. Here, in our present study, three PIK3CA genes termed SmPIK3CAa, SmPIK3CAb and SmPIK3CA-like were firstly identified in the genome of turbot S. maximus. Although these thr...
Source: Fish and Shellfish Immunology - June 15, 2019 Category: Biology Source Type: research

The protective effect of fish-derived cathelicidins on bacterial infections in zebrafish, Danio rerio
Publication date: Available online 13 June 2019Source: Fish & Shellfish ImmunologyAuthor(s): Chen Chen, Aili Wang, Fen Zhang, Minghui Zhang, Huaixin Yang, Jianan Li, Pengchao Su, Yan Chen, Haining Yu, Yipeng WangAbstractAntibiotic-resistant bacteria are severe threats to aquaculture industry. Boosting and modulating host immune responses has been proved to be an effective strategy to combat with bacterial infections and there is an urgent need for novel immunomodulators. Cathelicidins is an important family of host defense peptides (HDPs) that possess direct antimicrobial activities and potent immunomodulatory properti...
Source: Fish and Shellfish Immunology - June 13, 2019 Category: Biology Source Type: research

Improvements in the growth performance, immunity, disease resistance, and gut microbiota by the probiotic Rummeliibacillus stabekisii in Nile tilapia (Oreochromis niloticus)
Publication date: Available online 13 June 2019Source: Fish & Shellfish ImmunologyAuthor(s): Tan Herng Yih, Sai-Wei Chen, Shao-Yang HuAbstractThe application of probiotics as an eco-friendly alternative to antibiotics is an emerging strategy for sustainable aquaculture. In the present study, Rummeliibacillus stabekisii was isolated from the gut of Nile tilapia, and the effects of R. stabekisii on the growth, innate immunity, disease resistance, and gut microbiota of Nile tilapia (Oreochromis niloticus) were investigated. The results showed significantly increased weight gain (WG), feed conversion ratio (FCR), and feed ...
Source: Fish and Shellfish Immunology - June 13, 2019 Category: Biology Source Type: research

Effect of in-situ and ex-situ biofloc on immune response of Genetically Improved Farmed Tilapia
This study suggests the adoption of in-situ (T1) based biofloc method to obtain better performance of GIFT Tilapia culture. (Source: Fish and Shellfish Immunology)
Source: Fish and Shellfish Immunology - June 13, 2019 Category: Biology Source Type: research

Individual and combined effects of ammonia-N and sulfide on the immune function and intestinal microbiota of pacific white shrimp Litopenaeus vannamei
In this study, we explored the individual and combined effects of ammonia-N and sulfide stress (1 mg/L sulfide and 15 mg/L ammonia-N) on the oxidation resistance, immune response and intestinal health of Litopenaeus vannamei during 72 h exposure. The total antioxidant capacity (T-AOC), malonaldehyde (MDA) and nitric oxide (NO) content, superoxide dismutase (SOD) and catalase activity (CAT), the immune-relative gene (caspase-3, hsp70 and IMD) expression in hepatopancreas and intestine of L.vannamei and the intestinal microbiota were measured. The result showed that MDA and NO contents in hepatopancreas of L. vannamei ...
Source: Fish and Shellfish Immunology - June 12, 2019 Category: Biology Source Type: research

Promiscuous T cell epitopes boosts specific IgM immune response against a P0 peptide antigen from sea lice in different teleost species
Publication date: Available online 11 June 2019Source: Fish & Shellfish ImmunologyAuthor(s): Yeny Leal, Janet Velazquez, Liz Hernandez, Jaya Kumari Swain, Alianet Rodríguez Rodríguez, Rebeca Martínez, Claudia García, Yassel Ramos, Mario Pablo Estrada, Yamila CarpioAbstractThe development of vaccines employing conserved protein antigens, for instance ribosomal protein P0, has as disadvantage the high degree of identity between pathogen and host proteins due to possible induction of tolerance or auto antibodies in the host organism. To overcome this drawback, peptide-based vaccines have been d...
Source: Fish and Shellfish Immunology - June 12, 2019 Category: Biology Source Type: research

Zebrafish FGFR3 is a negative regulator of RLR pathway to decrease IFN expression
Publication date: Available online 11 June 2019Source: Fish & Shellfish ImmunologyAuthor(s): Shu-Bo Liu, Long-Feng Lu, Xiao-Bing Lu, Shun Li, Yong-An ZhangAbstractFibroblast growth factor receptor (FGFR) 3 is one of the four distinct membrane-spanning tyrosine kinases required for proper skeletal development. In fish, the role of FGFR3 is still unclear. In this article, we reveal that zebrafish FGFR3 is a negative regulator of interferon (IFN) production in the innate immune response by suppressing the activity of TANK-binding kinase 1 (TBK1) in the process of virus infection. qPCR experiments demonstrate that the tran...
Source: Fish and Shellfish Immunology - June 12, 2019 Category: Biology Source Type: research

Deiminated proteins in extracellular vesicles and plasma of nurse shark (Ginglymostoma cirratum) - Novel insights into shark immunity
In this study, post-translationally deiminated proteins and extracellular vesicles (EVs) are described for the first time in shark plasma. We report a poly-dispersed population of shark plasma EVs, positive for phylogenetically conserved EV-specific markers and characterised by TEM. In plasma, 6 deiminated proteins, including complement and immunoglobulin, were identified, whereof 3 proteins were found to be exported in plasma-derived EVs. A PAD homologue was identified in shark plasma by Western blotting and detected at expected 70 kDa size. Deiminated histone H3, a marker of neutrophil extracellular trap formation, was...
Source: Fish and Shellfish Immunology - June 12, 2019 Category: Biology Source Type: research

Phytochemical composition and immunobiological activity of Hawthorn Crataegus mexicana nanoencapsulated in Longfin yellowtail Seriola rivoliana leukocytes
Publication date: Available online 12 June 2019Source: Fish & Shellfish ImmunologyAuthor(s): Martha Reyes-Becerril, Alma Martínez-Preciado, Crystal Guluarte, Kevyn Guerra, Dariel Tovar-Ramirez, María Esther Macias, Carlos AnguloAbstractThe aims of this study were to determine the presence of phenolic compounds in Hawthorn Crataegus mexicana, species native to Mexico, nanoencapsulated (CmNano) with maltodextrin at 100 and 170 °C (CmNano100 and CmNano170) and its antioxidant and immunological effects in Longfin yellowtail Seriola rivoliana leukocytes. The phytochemical study revealed an important leve...
Source: Fish and Shellfish Immunology - June 12, 2019 Category: Biology Source Type: research

Dietary nano cerium oxide promotes growth, relieves ammonia nitrogen stress, and improves immunity in crab (Eriocheir sinensis)
In this study, the beneficial effects of dietary nano cerium oxide (nano CeO2) as a potent antioxidant were examined in the Chinese mitten crab (Eriocheir sinensis). Crabs were fed a diet supplemented with 0, 0.2, 0.4, 0.8, 1.6, 3.2, 6.4, or 12.8 mg/kg nano CeO2 for 60 d. The optimum supplementation level of nano CeO2 that significantly increased weight gain rate and decreased feed coefficient was 0.8 mg/kg. This level also offered immune protection when crabs were kept under ammonia nitrogen stress and/or exposed to pathogen infection (Aeromonas hydrophila). Supplementation with 0.8 mg/kg of CeO2 (i) relieved path...
Source: Fish and Shellfish Immunology - June 12, 2019 Category: Biology Source Type: research

Selective expression of a “correct cloud” of Dscam in crayfish survivors after second exposure to the same pathogen
Publication date: Available online 12 June 2019Source: Fish & Shellfish ImmunologyAuthor(s): Tze Hann Ng, Ramya Kumar, Kantamas Apitanyasai, Shu-Ting He, Shen-Po Chiu, Han-Ching WangAbstractArthropod hypervariable Dscam (Down syndrome cell adhesion molecule) may be involved in adaptive-like immune characteristics, namely immune priming, enabling the host to “learn” and “remember” pathogens previously encountered in arthropods. However, expression of Dscam in immune-primed arthropods after a second challenge has apparently not been confirmed. Herein, working with Dscam of Australian freshwater cr...
Source: Fish and Shellfish Immunology - June 12, 2019 Category: Biology Source Type: research

Molecular characterization of the NK-lysin in a teleost fish, Boleophthalmus pectinirostris: Antimicrobial activity and immunomodulatory activity on monocytes/macrophages
In conclusion, our study reveals that BpNKL plays a role against E. tarda infection in the mudskipper by not only directly killing bacteria but also through an immunomodulatory activity on MO/MФ. (Source: Fish and Shellfish Immunology)
Source: Fish and Shellfish Immunology - June 12, 2019 Category: Biology Source Type: research

Comprehensive transcriptome analysis reveal key molecular events in the pearl oyster after pre-grafting conditioning
Publication date: Available online 10 June 2019Source: Fish & Shellfish ImmunologyAuthor(s): Yu Jiao, Shuai Yang, Guanjie Min, Yuting Zhang, Xiaodong Du, Qingheng WangAbstractPre-grafting conditioning is a crucial procedure before transplant surgery during pearl production. To investigate the molecular response of the pearl oyster Pinctada fucata martensii to conditioning, we constructed two hemocyte transcriptomes from pearl oysters with and without conditioning. A total of 134,222,686 raw reads were generated and assembled using the reference genome of the pearl oyster. Transcriptome analysis revealed 3,074 different...
Source: Fish and Shellfish Immunology - June 12, 2019 Category: Biology Source Type: research

Growth performance and immune status in common carp Cyprinus carpio as affected by plant oil-based diets complemented with β–glucan
In conclusion, the results show that common carp fed plant oil-based diets are able to produce substantial amounts of LC-PUFA for sustaining growth rate, immune status and disease resistance similar to fish fed a fish oil-based diet. The differences in the production capacity of LC-PUFAs by the two plant oil-based diets were associated to a differential activation of some immune pathways, explaining how the use of these oils did not affect the overall immunocompetence of fish challenged with bacterial infection. Moreover, plant oil-based diets did not induce substantial negative effects on the immunomodulatory action of &b...
Source: Fish and Shellfish Immunology - June 12, 2019 Category: Biology Source Type: research

Molecular characterization and functional analysis of glutathione S-transferase kappa 1 (GSTκ1) from the big belly seahorse (Hippocampus abdominalis): Elucidation of its involvement in innate immune responses
In this study, a kappa class of GST was identified from the big belly seahorse (Hippocampus abdominalis) (HaGSTκ1) and its biochemical and functional properties were analyzed. HaGSTκ1 has 231 amino acids encoded by a 696 bp open reading frame (ORF). The protein has a predicted molecular mass of 26.04 kDa and theoretical isoelectric point (pI) of 8.28. It comprised a thioredoxin domain, disulfide bond formation protein A (DsbA) general fold, and Ser15 catalytic site as well as GSH-binding and polypeptide-binding sites. Phylogenetic analysis revealed that HaGSTκ1 is closely clustered with the kappa class ...
Source: Fish and Shellfish Immunology - June 12, 2019 Category: Biology Source Type: research

Nanoliposomes encapsulating immunostimulants modulate the innate immune system and elicit protection in zebrafish larvae
Publication date: Available online 11 June 2019Source: Fish & Shellfish ImmunologyAuthor(s): Jie Ji, Susana Merino, Juan Tomás Magaña, Nerea RoherAbstractHere we present immunostimulant-loaded nanoliposomes (NLc) as a strategy to protect zebrafish larvae against bacterial infection. The NLc encapsulate crude lipopolysaccharide (LPS) from E. coli and polyinosinic:polycytidylic acid (Poly I:C), a synthetic analogue of viral dsRNA. Fluorescently-labeled NLc were ingested by zebrafish larvae 4 days post fertilization, when administrated by bath immersion, and accumulated in the intestine. RT-qPCR analysis sho...
Source: Fish and Shellfish Immunology - June 12, 2019 Category: Biology Source Type: research

Heritability of immunity traits and disease resistance of bighead catfish, Clarias macrocephalus Günther, 1864
In this study, the genetic parameters of resistance to Aeromonas hydrophila were reported for the first time in the bighead catfish, Clarias macrocephalus Günther, 1864 which is an important parental species for the production of the commercially important hybrid C. macrocephalus × C. gariepinus. The analyses were performed on 736 data records obtained from 74 full-sib families (31 half-sib families) produced by factorial mating design. The results showed that the heritability of survival rate after disease (Aeromonas hydrophila) challenge (intraperitoneal injection with 0.1 ml containing 1 × 106...
Source: Fish and Shellfish Immunology - June 8, 2019 Category: Biology Source Type: research

The influence of surface proteins on the probiotic effects of Lactobacillus pentosus HC-2 in the Litopenaeus vannamei hepatopancreas
Publication date: September 2019Source: Fish & Shellfish Immunology, Volume 92Author(s): Yang Du, Mengqiang Wang, Baojie Wang, Mei Liu, Keyong Jiang, Lei WangAbstractOur previous work showed that Lactobacillus pentosus HC-2 has high antibacterial and adhesion activity, and as a probiotic could improve the nutrients and immunomodulatory effects in the Litopenaeus vannamei farming. In order to further investigate the influence of HC-2 surface protein on its probiotic effects, the immune and digestion related genes expression and enzymes activities, the colonization numbers of HC-2, and the histologic characteristics were...
Source: Fish and Shellfish Immunology - June 8, 2019 Category: Biology Source Type: research