Protec™ improves innate immune response and specific antibody response against Lactococcus garvieae in rainbow trout (Oncorhynchus mykiss)

Publication date: July 2019Source: Veterinary Immunology and Immunopathology, Volume 213Author(s): Chiara Bulfon, Valentina Pacorig, Massimo Sarti, Umberto Luzzana, Marco Galeotti, Donatella VolpattiAbstractProtec™ is a commercial aquafeed (Skretting Italia) containing a combination of glucans, vitamin C, vitamin E and zinc (immune support pack). No research information concerning its capability to improve fish immune response is available, so in this study the potential immunomodulatory effects of Protec™ were investigated in rainbow trout (Oncorhynchus mykiss). Head kidney (HK) leukocytes from adult fish (100 g, n = 6) were in vitro incubated with Protec™ immune support pack resulting in significantly higher respiratory burst activity and proliferation. Specifically, sonicated Protec™ immune support pack (160 μg/ml) induced a respiratory burst response similar to that promoted by zymosan and lipopolysaccharide (LPS), while non-sonicated Protec™ immune support pack induced a response comparable to that of cells stimulated with phorbol myristate acetate (PMA). Moreover, the proliferation of leukocytes exposed to sonicated Protec™ immune support pack (20 μg/ml) was significantly higher than that of cells stimulated with zymosan, and it was comparable to the proliferation of cells stimulated with phytohaemagglutinin (PHA) and LPS. Afterwards, a feeding trial was performed in a rainbow trout farm. Two groups of juvenile rainbow trout (10 g) were acclima...
Source: Veterinary Immunology and Immunopathology - Category: Veterinary Research Source Type: research