Editorial Board
Publication date: September 2018Source: Veterinary Immunology and Immunopathology, Volume 203Author(s): (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - September 21, 2018 Category: Veterinary Research Source Type: research

Parallel testing increases detection of Mycobacterium bovis-infected African buffaloes (Syncerus caffer)
Publication date: Available online 18 September 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Netanya Bernitz, Wynand J. Goosen, Charlene Clarke, Tanya J. Kerr, Roxanne Higgitt, Eduard O. Roos, David V. Cooper, Robin M. Warren, Paul D. van Helden, Sven D.C. Parsons, Michele A. MillerAbstractThe diagnosis of Mycobacterium bovis (M. bovis) infection in African buffaloes (Syncerus caffer) relies on detection of the cell-mediated immune response to M. bovis antigens using the single comparative intradermal tuberculin test (SCITT) or interferon gamma release assays (IGRAs). The aim of the present study was to ...
Source: Veterinary Immunology and Immunopathology - September 19, 2018 Category: Veterinary Research Source Type: research

Recombinant ferritin-H induces immunosuppression in European sea bass larvae (Dicentrarchus labrax) rather than immunostimulation and protection against a Vibrio anguillarum infection
In this study, we evaluated if the recombinant sea bass ferritin-H could stimulate the innate immune system of gnotobiotic European sea bass larvae resulting in protection against a V. anguillarum challenge. We also evaluated the effect of a V. anguillarum infection on the transcription of immune-related genes in gnotobiotic European sea bass larvae. Recombinant sea bass ferritin-H was produced, encapsulated in calcium alginate microparticles and orally delivered to sea bass larvae at seven days after hatching. Our results showed V. anguillarum caused an acute infection, resulting in high mortality. The infection significa...
Source: Veterinary Immunology and Immunopathology - September 14, 2018 Category: Veterinary Research Source Type: research

C-C motif chemokine ligand (CCL) production in equine peripheral blood mononuclear cells identified by newly generated monoclonal antibodies
Publication date: Available online 11 September 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Christiane L. Schnabel, Michelle Wemette, Susanna Babasyan, Heather Freer, Cynthia Baldwin, Bettina WagnerAbstractChemokines are soluble molecules directing immune cell trafficking and homing, mediating inflammation, and initiating immune responses to infection. In horses, the analysis of chemokines has been limited by the lack of specific antibodies. We generated mAbs specific for the equine C-C motif chemokine ligands (CCL) CCL2 (MCP-1), CCL3 (MIP-1α), CCL5 (RANTES) and CCL11 (eotaxin) using hybridoma tec...
Source: Veterinary Immunology and Immunopathology - September 12, 2018 Category: Veterinary Research Source Type: research

Identification of targets of monoclonal antibodies that inhibit adhesion and growth in Mycoplasma mycoides subspecies mycoides
Publication date: Available online 10 September 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Racheal Aye, Yenehiwot Berhanu Weldearegay, Harrison Osundwa Lutta, Francis Chuma, Andreas Pich, Joerg Jores, Jochen Meens, Jan NaessensAbstractMycoplasma mycoides subspecies mycoides (Mmm) adhesion is tissue and host specific. Inhibition of adhesion will prevent Mmm from binding to lung cells and hence prevent colonization and disease. The aim of this study was to develop a panel of Mmm monoclonal antibodies against Mmm and use these antibodies to investigate their inhibitory effect on the adherence of Mmm to bo...
Source: Veterinary Immunology and Immunopathology - September 11, 2018 Category: Veterinary Research Source Type: research

Antibody-mediated bacterial killing of Ichthyobacterium seriolicida in Japanese amberjack
In this study, fish were passively immunized to determine whether serum antibody is involved in protection against BHJ. The susceptibility of I. seriolicida to the bactericidal activity of Japanese amberjack serum was also investigated. In passive immunization tests, significantly lower mortality was noted in fish that received convalescent serum. Bacteria were killed when exposed to convalescent serum but not serum from naïve fish. Electron microscopic analyses showed that I. seriolicida cells were morphologically altered by reaction with convalescent serum. Naïve fish serum became bactericidal upon addition of ...
Source: Veterinary Immunology and Immunopathology - August 30, 2018 Category: Veterinary Research Source Type: research

Comment on “The domestic cat antibody responseto FHV-1 increases with age”
Publication date: September 2018Source: Veterinary Immunology and Immunopathology, Volume 203Author(s): James A. Roth (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - August 25, 2018 Category: Veterinary Research Source Type: research

Pharmacokinetics of human recombinant C1-esterase inhibitor and development of anti-drug antibodies in healthy dogs
This study aimed to characterize the pharmacokinetics of a single 500 IU IV dose of rC1-INH in 8 healthy beagle dogs, evaluate the dogs for any adverse effects of drug administration, and determine whether rC1-INH administration induces anti-drug antibody formation. Serum rC1-INH concentrations were measured using a commercial functional ELISA at baseline and at 10, 20, 40, 60, 80, 100, 120, 240, 360, 480, 600, 720, 960, and 1440 minutes post drug administration. Complete blood counts were conducted at baseline, 720 and 1440 minutes. Western blot analysis, using rC1-INH as the target antigen was used to detec...
Source: Veterinary Immunology and Immunopathology - August 24, 2018 Category: Veterinary Research Source Type: research

Reply to “The domestic cat antibody response to FHV-1 increases with age”
Publication date: Available online 20 August 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): James A. Roth (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - August 21, 2018 Category: Veterinary Research Source Type: research

Enterotoxic effects of Aeromonas hydrophila infection in the catfish, Clarias gariepinus: Biochemical, histological and proteome analyses
In this study, the impact of A. hydrophila infection in the air-breathing catfish, Clarias gariepinus was analyzed using a multidimensional approach. Aeromonas hydrophila (1 × 107 cells) was injected into C. gariepinus intraperitoneally at an ambient temperature with periodical monitoring for morphological changes. After 7 days post-infection, tissue samples of the gills, liver, intestine, and kidney were subjected to biochemical, histological, transmission electron microscope (TEM) and proteomic analyses. Observed results indicated distinct morphological changes with the significant increase of ROS and...
Source: Veterinary Immunology and Immunopathology - August 20, 2018 Category: Veterinary Research Source Type: research

Detection of anti-neutrophil cytoplasmic antibodies in dogs with immune-mediated inflammatory diseases
Publication date: Available online 16 August 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Takashi Tamamoto, Aki Nagao, Mizuki SuganoAbstractAnti-neutrophil cytoplasmic antibody (ANCA) is a type of autoantibody against neutrophil cytoplasm. In veterinary medicine, few studies have reported the detection of ANCA in dogs, and most of these studies were performed in dogs with inflammatory bowel disease (IBD). The aim of this study was to evaluate whether ANCA is detected in dogs with immune-mediated inflammatory diseases (IMIDs) other than IBD. Serum samples were collected before treatment initiation from 40...
Source: Veterinary Immunology and Immunopathology - August 16, 2018 Category: Veterinary Research Source Type: research

Benzo(a)pyrene suppresses tracheal antimicrobial peptide gene expression in bovine tracheal epithelial cells
Publication date: Available online 16 August 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Laura A. Bourque, Stephen Raverty, Carmon Co, Brandon N. Lillie, Pierre-Yves Daoust, Mary Ellen Clark, Jeff L. CaswellAbstractRespiratory disease is an important cause of morbidity and mortality in cetaceans, which are also threatened by environmental degradation caused by crude oil spills. Following oil spills, cetaceans at the water surface may inhale droplets of oil containing toxic polycyclic aromatic hydrocarbons (PAHs), which could potentially alter respiratory immunity via activation of the aryl hydrocarbon r...
Source: Veterinary Immunology and Immunopathology - August 16, 2018 Category: Veterinary Research Source Type: research

Effects of a pathogenic ETEC strain and a probiotic Enterococcus faecium strain on the inflammasome response in porcine dendritic cells
Publication date: Available online 15 August 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Henriette Loss, Jörg R. Aschenbach, Friederike Ebner, Karsten Tedin, Ulrike LodemannAbstractDendritic cells (DC) are crucial for maintaining intestinal homeostasis and generating proper immune responses to bacteria occurring in the gut. Microbial stimuli can be recognized by intracellular receptors called inflammasomes, e.g., nucleotide oligomerization domain (NOD)-like receptor protein 3 (NLRP3). The aim of the present study was to unravel the inflammasome response of porcine monocyte-derived DC (MoDC). We inv...
Source: Veterinary Immunology and Immunopathology - August 16, 2018 Category: Veterinary Research Source Type: research

The expression of Indoleamine 2, 3-dioxygenase (IDO) is reduced in granulomas from BCG vaccinated cattle compared to granulomas from unvaccinated controls after experimental challenge with Mycobacterium bovis
Publication date: Available online 13 August 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Waldo L. Garcia-Jimenez, Bernardo Villarreal-Ramos, Duncan Grainger, R. Glyn Hewisnon, Hans M. Vordermeier, Francisco J. SalgueroAbstractBovine tuberculosis (bTB), mainly caused by Mycobacterium bovis (M. bovis), is a major economic disease of livestock worldwide. Vaccination is considered as a potentially sustainable adjunct to the current control strategy. Cattle vaccination with the live attenuated M. bovis bacillus Calmette-Guerin (BCG) confers variable protection; the reasons for this variability are not unders...
Source: Veterinary Immunology and Immunopathology - August 14, 2018 Category: Veterinary Research Source Type: research

Enhancing purification and plasma stability of porcine interferon-α/γ by fusion to elastin-like polypeptide
In this study, we evaluated the feasibility of ELP fusion for simple purification and half-life extension of recombinant porcine IFNs (rPoIFNs). After construction of five different fusion expression vectors, we optimized the conditions for soluble protein expression and purification. SDS-PAGE analysis showed that, unlike PoIFNα-His and PoIFNγ-His, PoIFNα-ELP, ELP-PoIFNα and PoIFNαγ-ELP were expressed mainly as soluble proteins at 20 ℃. The optimal conditions for the inverse transition cycling (ITC) of three ELP fusion proteins were 2 M NaCl at 28 ℃. After two rounds of ITC, t...
Source: Veterinary Immunology and Immunopathology - August 14, 2018 Category: Veterinary Research Source Type: research

Blood serum acute phase proteins and iron dynamics during acute phase response of Salmonella enterica Serotype Dublin experimentally infected buffalo calves
Publication date: Available online 4 August 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): André M. Santana, Daniela G. Silva, Funmilola C. Thomas, Priscila A. Bernardes, Lucas J.L. Pizauro, Clarissa H. Santana, Richard J.S. Burchmore, Peter D. Eckersall, José J. FagliariAbstractThe study aimed to evaluate clinical signs, blood serum acute phase proteins (APP) and iron dynamics during the acute phase response (APR) of Salmonella Dublin experimentally infected Murrah buffalo calves. Six buffalo calves constituted the control group (CNT) and six were orally inoculate with 108 CFU of S. Dublin (...
Source: Veterinary Immunology and Immunopathology - August 4, 2018 Category: Veterinary Research Source Type: research

Editorial Board
Publication date: August 2018Source: Veterinary Immunology and Immunopathology, Volume 202Author(s): (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - August 4, 2018 Category: Veterinary Research Source Type: research

Resveratrol administration increases phagocytosis, decreases oxidative burst, and promotes pro-inflammatory cytokine production in healthy dogs
The objective of this study was to determine the short-term effect of resveratrol on the healthy canine innate immune system in vivo. The hypothesis was that resveratrol would spare phagocytosis, depress the vigor of PMN oxidative burst, and result in a proinflammatory stimulated leukocyte cytokine profile in vivo. In an open-label study, whole blood was collected from 12 healthy, adult client-owned dogs on day 0 and 3. Six dogs received resveratrol, 200 mg kg-1, orally once daily for three days and six dogs served as controls with no supplement administered. Phagocytosis, oxidative burst and pathogen associa...
Source: Veterinary Immunology and Immunopathology - July 31, 2018 Category: Veterinary Research Source Type: research

Molecular cloning and expression analyses of immunoglobulin tau heavy chain (IgT) in turbot, Scophthalmus maximus
Publication date: Available online 30 July 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Xiaoqian Tang, Yang Du, Xiuzhen Sheng, Jing Xing, Wenbin ZhanAbstractImmunoglobulins (Igs) are humoral mediators playing the prevailing role in the innate and adaptive immunities of jawed vertebrates and provide obligatory duties to protect the organism from a wide variety of pathogens. In the present study, the membrane-bound and secretory immunoglobulin T (mIgT and sIgT) genes of turbot (Scophthalmus maximus) were cloned for the first time with the intention of better understanding the IgT functions. The mIgT cDNA i...
Source: Veterinary Immunology and Immunopathology - July 30, 2018 Category: Veterinary Research Source Type: research

Lack of cross-reactivity of human and porcine reagents to quantify manatee (Trichechus manatus) cytokines
Publication date: Available online 29 July 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Milton Levin, Lindsay Jasperse, Erika Gebhard, Estelle Rousselet, Catherine WalshAbstractVeterinary medical examinations, including both physical examination and diagnostic tests, are important to monitor the health of both managed-care and wild marine mammals. However, limited species-specific reagents and assays are available that may contribute to a broader medical examination. This project evaluated if commercially available human and porcine antibodies and reagents would cross-react with manatee (Trichechus manat...
Source: Veterinary Immunology and Immunopathology - July 30, 2018 Category: Veterinary Research Source Type: research

Use of ELISA and Western blot for serological detection of antibodies to E-S antigens of Trichinella spiralis muscle larvae in sera of swine experimentally infected with Trichinella spiralis
Publication date: Available online 29 July 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Michał Gondek, Justyna Bień, Zygmunt NowakowskiABSTRACTThe aim of the present study was to evaluate the detection of trichinellosis using ELISA, and to determine the degree of variation of IgG antibodies against excretory-secretory (ES) antigens of T. spiralis muscle larvae. Ten young Polish Large White native breed pigs were experimentally inoculated with a low dose of 300 invasive Trichinella spiralis (T. spiralis) muscle larvae (ML). Pig sera were collected at 7 and 9 days prior to the experimental infection with...
Source: Veterinary Immunology and Immunopathology - July 30, 2018 Category: Veterinary Research Source Type: research

Sheep and cattle exposed to Mycobacterium avium subspecies paratuberculosis exhibit altered total serum cholesterol profiles during the early stages of infection
This study sought to evaluate the levels of total serum cholesterol in the host following exposure to MAP. Blood samples were collected from both sheep and cattle prior to experimental challenge with MAP and at monthly intervals post-challenge. Total serum cholesterol levels in sheep challenged with MAP were significantly elevated at 9 weeks post-inoculation (wpi) in comparison to controls. When stratified based on disease outcome, there was no significant difference in serum cholesterol at the timepoints examined between MAP exposed sheep that were susceptible and those that were resistant to Johne’s disease. There ...
Source: Veterinary Immunology and Immunopathology - July 21, 2018 Category: Veterinary Research Source Type: research

Effect of antigen challenge on dynamics of cd62p and cd41/61 expression on platelets in horses with recurrent airway obstruction (rao)
Publication date: Available online 18 July 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Alicja Iwaszko, Hieronim Borowicz, Stanislaw Graczyk, Malwina Slowikowska, Aleksandra Pliszczak-Krol, Artur NiedzwiedzAbstractRecurrent airway obstruction (RAO), also known as heaves, is an allergic respiratory condition that develops in horses following an exposure to aeroallergens in hay and straw. This is manifested by airway hyperreactivity, inflammation, bronchoconstriction, as well as a leukocyte and platelet infiltration into the airways. Platelet activation and an increase in circulating platelet-leukocyte agg...
Source: Veterinary Immunology and Immunopathology - July 19, 2018 Category: Veterinary Research Source Type: research

Research paper: Sheep and cattle exposed to Mycobacterium avium subspecies paratuberculosis exhibit altered total serum cholesterol profiles during the early stages of infection
This study sought to evaluate the levels of total serum cholesterol in the host following exposure to MAP. Blood samples were collected from both sheep and cattle prior to experimental challenge with MAP and at monthly intervals post-challenge. Total serum cholesterol levels in sheep challenged with MAP were significantly elevated at 9 weeks post-inoculation (wpi) in comparison to controls. When stratified based on disease outcome, there was no significant difference in serum cholesterol at the timepoints examined between MAP exposed sheep that were susceptible and those that were resistant to Johne’s disease. There ...
Source: Veterinary Immunology and Immunopathology - July 18, 2018 Category: Veterinary Research Source Type: research

Acidic polypeptides A1a, A3 and A4 of Gly m 6 (glycinin) are allergenic for piglets
Publication date: August 2018Source: Veterinary Immunology and Immunopathology, Volume 202Author(s): Shugui Zheng, Guixin Qin, Jing Chen, Fei ZhangAbstractGly m 6 (glycinin) is one of the major antigenic proteins in soybeans responsible for transient hypersensitivity to soybean meal in weaned piglets. The globulin is a hexamer consisting of subunits containing basic and acidic polypeptides. Multiple acidic polypeptides have long been demonstrated to be allergens for humans and play a key role in the overall allergenicity of Gly m 6. To date, knowledge on the allergenicity of the acidic polypeptides for piglets is very limi...
Source: Veterinary Immunology and Immunopathology - July 18, 2018 Category: Veterinary Research Source Type: research

Detection of IgM-rheumatoid factor and anti-citrullinated protein antibodies in healthy horses and their comparison
Publication date: Available online 11 July 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): R. Hoikhman, H. Kudlackova, V. Babak, M. Faldyna, P. JahnAbstractRheumatoid factor (RF) is used in human and veterinary medicine in the form of IgM RF traditionally to support the diagnosis of rheumatoid arthritis (RA). In the latest diagnostic criteria, presence of anti - citrullinated protein antibodies (ACPA) was added to the grading system for the diagnosis of RA in humans. A change which is not integrated or routinely used in veterinary medicine. The criteria changed partly because of RF’s diagnostic shortc...
Source: Veterinary Immunology and Immunopathology - July 12, 2018 Category: Veterinary Research Source Type: research

Characterization of the canine immunoglobulin heavy chain repertoire by next generation sequencing
The objective of this study was to utilize NGS for comprehensive and unbiased analysis of the IGH repertoire in healthy dogs. First, the IGH locus was searched in silico for previously unidentified genes. Second, IGH transcripts from major lymphoid organs were amplified using a 5’RACE approach without V/J primer bias. Third, amplicons were sequenced on an Illumina MiSeq platform, and data were analyzed using the ARResT/Interrogate platform. Data analysis included V/J usage, V-J pairing biases, isotype frequency, CDR3 diversity, convergent recombination, and public repertoires. The results of this study provide a comp...
Source: Veterinary Immunology and Immunopathology - July 11, 2018 Category: Veterinary Research Source Type: research

Acidic polypeptides A1a, A3, and A4 of Gly m 6 (glycinin) are allergenic for piglets
Publication date: Available online 6 June 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Shugui Zheng, Guixin Qin, Jing Chen, Fei ZhangAbstractGly m 6 (glycinin) is one of the major antigenic proteins in soybeans responsible for transient hypersensitivity to soybean meal in weaned piglets. The globulin is a hexamer consisting of subunits containing basic and acidic polypeptides. Multiple acidic polypeptides have long been demonstrated to be allergens for humans and play a key role in the overall allergenicity of Gly m 6. To date, knowledge on the allergenicity of the acidic polypeptides for piglets is very...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Glucocorticosteroids administration is associated with increased regulatory T cells in equine asthmatic lungs
Publication date: July 2018Source: Veterinary Immunology and Immunopathology, Volume 201Author(s): Roxane Boivin, Amandine Vargas, Patricia Cano, Jean-Pierre LavoieAbstractRecurrent inflammation in severe equine asthma causes a remodeling of the airways leading to incompletely reversible airway obstruction. Despite the improvement of clinical signs and lung function with glucocorticoids (GC), inflammation, translated by an increased percentage of neutrophils, persists in the airways. Regulatory T cells (Treg) have been shown to have anti-inflammatory properties and play an important role in balancing the immune response by...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Establishment and characterization of a cell line from a feline histiocytic sarcoma
Publication date: July 2018Source: Veterinary Immunology and Immunopathology, Volume 201Author(s): Ryo Miyamoto, Sena Kurita, Hiroyuki Tani, Masato Kobayashi, Soudai Sugiura, Kae Shigihara, Yuka Sato, Yoshikazu Tanaka, Kyoichi Tamura, Makoto BonkobaraAbstractFeline histiocytic sarcoma (HS) is an aggressive and uncommon tumor originating from dendritic cells/macrophages. Here, a feline HS cell line, FHS-1, was established from a case of feline HS and characterized. Immunohistochemically, FHS-1 cells were positive for vimentin and Iba-1, and negative for MHC class II and CD163. FHS-1 cells were positive for α-naphthyl ...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Fatty and hydroxycarboxylic acid receptors: The missing link of immune response and metabolism in cattle
Publication date: July 2018Source: Veterinary Immunology and Immunopathology, Volume 201Author(s): P. Alarcon, C. Manosalva, M.D. Carretta, A.I. Hidalgo, C.D. Figueroa, A. Taubert, C. Hermosilla, M.A. Hidalgo, R.A. BurgosAbstractFatty and hydroxycarboxylic acids are one of the main intermediates of energy metabolism in ruminants and critical in the milk production of cattle. High production demands on a dairy farm can induce nutritional imbalances and metabolism disorders, which have been widely associated with the onset of sterile inflammatory processes and increased susceptibility to infections. The literature suggests t...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Corrigendum to “Detection of Mycobacterium bovis infection in African buffaloes (Syncerus caffer) using QuantiFERON®-TB Gold (QFT) tubes and the Qiagen cattletype® IFN-gamma ELISA” [J. Vet. Immunol. Immunopathol. 196 (2018) 48–52]
Publication date: July 2018Source: Veterinary Immunology and Immunopathology, Volume 201Author(s): Netanya Bernitz, Charlene Clarke, Eduard O. Roos, Wynand J. Goosen, David Cooper, Paul D. van Helden, Sven D.C. Parsons, Michele A. Miller (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Editorial Board
Publication date: July 2018Source: Veterinary Immunology and Immunopathology, Volume 201Author(s): (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Effects of early feeding and dietary interventions on development of lymphoid organs and immune competence in neonatal chickens: A review
Publication date: July 2018Source: Veterinary Immunology and Immunopathology, Volume 201Author(s): Khaled Taha-Abdelaziz, Douglas C. Hodgins, Aart Lammers, Tamiru Negash Alkie, Shayan SharifAbstractWith the ongoing intensification of the poultry industry and the continuous need to control pathogens, there is a critical need to extend our understanding of the avian immune system and the role of nutritional interventions on development of immune competence in neonatal chicks. In this review, we will focus on the ontogeny of the lymphoid organs during embryonic life and the first 2 weeks post-hatch, and how early feeding prac...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Antigen-specific interferon-gamma release is decreased following the single intradermal comparative cervical skin test in African buffaloes (Syncerus caffer)
Publication date: July 2018Source: Veterinary Immunology and Immunopathology, Volume 201Author(s): C. Clarke, D. Cooper, W.J. Goosen, R. McFadyen, R.M. Warren, P.D. van Helden, S.D.C Parsons, M.A. MillerAbstractEffective disease management of wildlife relies on the strategic application of ante-mortem diagnostic tests for early identification and removal of M. bovis-infected animals. To improve diagnostic performance, interferon-gamma release assays (IGRAs) are often used in conjunction with the tuberculin skin test (TST). Since buffaloes are major maintenance hosts of M. bovis, optimal application of bovine TB diagnostic ...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Polymorphonuclear cells and reactive oxygen species in contagious bovine pleuropneumonia: New insight from in vitro investigations
Publication date: July 2018Source: Veterinary Immunology and Immunopathology, Volume 201Author(s): Giovanni Di Teodoro, Giuseppe Marruchella, Francesco Mosca, Andrea Di Provvido, Flavio Sacchini, Pietro Giorgio Tiscar, Massimo ScacchiaAbstractReactive oxygen species (ROS) are suggested to play a role in the pathogenesis of contagious bovine pleuropneumonia, a severe respiratory disorder caused by Mycoplasma mycoides subsp. mycoides (Mmm). The present study investigated the generation of ROS by different strains of Mmm, as well as their effect on the oxidative response of bovine neutrophils. The production of ROS was indire...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Epitope mapping of Salmonella flagellar hook-associated protein, FlgK, with mass spectrometry-based immuno-capture proteomics using chicken (Gallus gallus domesticus) sera
Publication date: July 2018Source: Veterinary Immunology and Immunopathology, Volume 201Author(s): Hung-Yueh Yeh, Kyoko Kojima, James A. MobleyAbstractSalmonella, a Gram-negative rod, is the leading foodborne pathogen associated with human acute bacterial gastroenteritis worldwide. The Salmonella flagellum is responsible for bacterial movement, colonization and invasion in the host gastrointestinal tract. The flagellum has a complex structure, composed of more than 35 proteins. Among them, we were interested in the flagellar hook-associated protein (FlgK), which is an immunodominant protein in chickens. In this communicati...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Evaluating the cytokine profile of the WC1+ γδ T cell subset in the ileum of cattle with the subclinical and clinical forms of MAP infection
This study demonstrates changes in the cytokine mRNA expression profile of mucosal cells in the ileum, and specifically WC1+ γδ T cells, as cattle progress to the clinical disease. The change is characterized by an increase in expression of anti-inflammatory cytokines. (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Barley produced Culicoides allergens are suitable for monitoring the immune response of horses immunized with E. coli expressed allergens
Publication date: July 2018Source: Veterinary Immunology and Immunopathology, Volume 201Author(s): Sigridur Jonsdottir, Sara Bjork Stefansdottir, Sæmundur Bjarni Kristinarson, Vilhjalmur Svansson, Jon Mar Bjornsson, Arna Runarsdottir, Bettina Wagner, Eliane Marti, Sigurbjorg TorsteinsdottirAbstractInsect bite hypersensitivity is an allergic dermatitis of horses caused by bites of Culicoides midges. Sufficient amount of pure, endotoxin-free allergens is a prerequisite for development and monitoring of preventive and therapeutic allergen immunotherapy.Aims of the study were to compare the Culicoides nubeculosus (Cul n)...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Survey of cytokines in normal canine tears by multiplex analysis: A pilot study
ConclusionThis pilot study is the first to analyze cytokines in canine tears. This study demonstrated that IL-8 is consistently detected in both diluted and undiluted samples, but undiluted samples may be superior to 1:10 diluted samples for evaluation of other cytokines in canine tears. (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

IP-10: A potential biomarker for detection of Mycobacterium bovis infection in warthogs (Phacochoerus africanus)
In this study, we obtained blood samples from warthogs in bTB endemic areas to investigate biomarkers for detection of Mycobacterium bovis infection. Warthog whole blood was incubated in QuantiFERON® TB Gold In-Tube tubes and pathogen specific release of interferon gamma (IFN-γ) and interferon gamma induced protein 10 (IP-10) was measured by a sandwich enzyme-linked immunosorbent assay. Although we were unable to measure IFN-γ, we could successfully measure IP-10. The IP-10 assay was able to distinguish between M. bovis-infected and M. bovis-culture negative warthogs, within bTB endemic areas, with an assay...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Inflammasomes in livestock and wildlife: Insights into the intersection of pathogens and natural host species
We describe current gaps in our knowledge and highlight promising areas for future research. (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Effects of a P-class CpG-ODN administered by intramuscular injection on plasma cytokines and on white blood cells of healthy horses
Publication date: July 2018Source: Veterinary Immunology and Immunopathology, Volume 201Author(s): I. Tosi, F. Bureau, F. Farnir, J.M. Denoix, P. Lekeux, T. ArtAbstractCytosine-phosphate-guanosine (CpG-ODN) has been described as a potent immunostimulatory agent in different species. No study reported the effect of a P-class CpG when administered systemically in healthy horses. The aim of this study was to evaluate the tolerance and the effect of an intramuscularly administered P-class CpG-ODN on hematology and on plasma cytokines (IFN-α, IL-10, TNF-α, IFN-γ) in 8 healthy horses. Intra-muscular CpG-ODN or ...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Effect of Marek’s disease vaccines on interferon and toll like receptors when administered in ovo
Publication date: July 2018Source: Veterinary Immunology and Immunopathology, Volume 201Author(s): Isabel M. Gimeno, Ayanna Glaize, Aneg L. CortesAbstractThe effect of two Marek’s disease (MD) vaccines on the chicken embryo immune responses were evaluated. Transcription of interferon (IFN-α, IFN-β, IFN-λ, and IFN-γ) and interferon-I receptors (IFN-AR1 and IFN-AR2), as well as transcription of toll like receptors (TLR-3, TLR-7, and TLR-21) were evaluated in the bursa, thymus, spleen and lung of 1-day-old chickens that had been vaccinated with HVT, CVI988, or sham inoculated at embryonic day 18 ...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Pathological, immunological and parasitological study of sheep vaccinated with the recombinant protein 14-3-3z and experimentally infected with Fasciola hepatica
In this study, the immunogenicity and protective capacity of a new recombinant vaccine candidate, the rFh14-3-3z protein was analysed in sheep experimentally challenged with Fasciola hepatica, in terms of fluke burden, faecal egg counts, hepatic damage and humoral immune response. Three groups of 8 animals each were used for study, group 1 was immunised with the rFh14-3-3z in Montanide adjuvant, whereas group 2 and 3 remained as adjuvant control and infection control groups, respectively. The parasitological analysis showed that no significant reduction in fluke burden, fluke size and faecal egg counts was detected. The ex...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

CIRCULATING REGULATORY T CELLS (Treg), LEPTIN AND INDUCTION OF PROINFLAMMATORY ACTIVITY IN OBESE LABRADOR RETRIEVER DOGS
Publication date: Available online 7 July 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Anna Teresa Palatucci, Diego Piantedosi, Valentina Rubino, Angela Giovazzino, Jacopo Guccione, Vlenia Pernice, Giuseppina Ruggiero, Laura Cortese, Giuseppe TerrazzanoAbstractOver-nutrition and obesity have been associated with impaired immunity and low-grade inflammation in humans and mouse models. In this context, a causal role for unbalanced T regulatory cell (Treg)-dependent mechanisms has been largely suggested.Obesity is the most common nutritional disorder in dogs. However, it is not defined whether canine obesit...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Identification of genetic variation in equine collagenous lectins using targeted resequencing
This study contributes to the growing body of evidence that pooled, high-throughput sequencing is a viable strategy for cost-effective variant discovery. (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Beneficial and detrimental impact of transplanted canine adipose-derived stem cells in a virus-induced demyelinating mouse model
Publication date: Available online 8 July 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Florian Hansmann, Nicole Jungwirth, Ning Zhang, Thomas Skripuletz, Veronika Maria Stein, Andrea Tipold, Martin Stangel, Wolfgang BaumgärtnerAbstractIn recent years stem cell therapies have been broadly applied in various disease models specifically immune mediated and degenerative diseases. Whether adipose-derived stem cells might represent a useful therapeutic option in virus-triggered central nervous system diseases has not been investigated so far. Theiler’s murine encephalomyelitis (TME) and canine diste...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Assessment of Poly(ADP-ribose) Polymerase1 (PARP1) expression and activity in cells purified from blood and milk of dairy cattle
In conclusion, these findings suggest that PARP1 is activated during mastitis, which may prove to be a useful biomarker of mastitis. (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Expression of pattern recognition receptors in porcine uterine epithelial cells in vivo and in culture
Publication date: August 2018Source: Veterinary Immunology and Immunopathology, Volume 202Author(s): Glenn Hamonic, J. Alex Pasternak, Nikki M. Forsberg, Tobias Käser, Heather L. WilsonAbstractPreservation of a pathogen free uterine environment is critical for maintaining healthy swine herds with high reproductive performance. Considering that uterine epithelial cells are the most numerous and thus likely point of cellular contact for pathogens in the uterus, we hypothesize that these cells may be critical for activating the immune system to clear uterine infections. Although uterine epithelial cells have not been wel...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research