Editorial Board
Publication date: November 2018Source: Veterinary Immunology and Immunopathology, Volume 205Author(s): (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - November 18, 2018 Category: Veterinary Research Source Type: research

Expression of Toll-like receptors 2, 4 and 6 in the equine chorioallantois
This study examined the expression of TLR 2, 4 and 6 as transcript and protein in the placenta (chorioallantois) of 14 foals born alive. By PCR, all examined placental samples contained TLR 2, 4 and 6 transcripts. Using immunohistochemistry, trophoblasts and allantoic epithelium were immunopositive for TLR 2, 4 and 6 in all placental samples. The majority of placental samples contained TLR 4 and 6 positive stromal cells and vascular smooth muscle cells. Since these results confirm the expression of TLR 2, 4 and 6 in different cell populations of the equine placenta, they are the basis for studies into the pathogenesis of T...
Source: Veterinary Immunology and Immunopathology - November 17, 2018 Category: Veterinary Research Source Type: research

Evaluation of T regulatory lymphocytes and serum concentration of selected cytokines in dogs with perianal tumors
Publication date: Available online 12 November 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): U. Lisiecka, K. Kostro, K. Dudek, A. Brodzki, M. CzopAbstractThe purpose of this study was to determine concentrations of IL-2, IL-10, TGF-β1 in serum and T regulatory cell (Treg) percentage in peripheral blood of dogs with perianal tumours. Investigations were conducted on 32 male dogs of mixed breed. The animals were divided into 4 experimental groups and control group. The groups were established depending on the tumour malignancy degree and the type of dominant hormones. All measurements of serum cytokine...
Source: Veterinary Immunology and Immunopathology - November 14, 2018 Category: Veterinary Research Source Type: research

The porcine tonsils and Peyer’s patches: A stereological morphometric analysis in conventionally and artificially reared piglets
Publication date: Available online 13 November 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Sara Prims, Ben Jurgens, Charlotte Vanden Hole, Steven Van Cruchten, Chris Van Ginneken, Christophe CasteleynAbstractSelection for prolificacy in modern pig farming has resulted in increasing litter sizes. Since rearing large litters is challenging, artificial rearing of piglets with a milk replacer is an alternative strategy. It is hypothesized that the development of the piglets’ mucosa-associated lymphoid tissues (MALT) is affected by these artificial conditions. Therefore, the stereologically estimated v...
Source: Veterinary Immunology and Immunopathology - November 14, 2018 Category: Veterinary Research Source Type: research

A canine keratinocyte cell line expresses antimicrobial peptide and cytokine genes upon stimulation with bacteria, microbial ligands and recombinant cytokines
Publication date: Available online 13 November 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Suttiwee Chermprapai, Femke Broere, Yvette M. Schlotter, Edwin J.A. Veldhuizen, Victor P.M.G. RuttenAbstractKeratinocytes (KC) are the main cellular components of the stratum corneum that constitutes a solid physical skin barrier representing the first line of defense against pathogens. Moreover, KC are potent producers of inflammatory mediators and antimicrobial peptides (AMP) when activated through their pattern recognition receptors. In atopic dermatitis (AD) the protective skin barrier may be compromised due t...
Source: Veterinary Immunology and Immunopathology - November 14, 2018 Category: Veterinary Research Source Type: research

Melatonin enhances responsiveness to Dichelobacter nodosus vaccine in sheep and increases peripheral blood CD4 T lymphocytes and IgG-expressing B lymphocytes
Publication date: Available online 8 November 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Asunción Ramos, María Prado Míguez, Sara Morgado, Beatriz Sanchez-Correa, Juan J. Gordillo, Javier G. Casado, Raquel Tarazona, Sergio RegodónAbstractThe immunomodulatory functions mediated by melatonin support its use as vaccine adjuvant. Previously, we have demonstrated that melatonin enhances antibody responses in sheep vaccinated against Dichelobacter nodosus. Here, we analyze the effect of melatonin on T and B lymphocyte subsets in peripheral blood of sheep vaccinated against D. nodo...
Source: Veterinary Immunology and Immunopathology - November 9, 2018 Category: Veterinary Research Source Type: research

Piglet weight gain during the first two weeks of lactation influences the immune system development
Publication date: Available online 5 November 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Martin Lessard, Mylène Blais, Frédéric Beaudoin, Karine Deschene, Luca Lo Verso, Nathalie Bissonnette, Karoline Lauzon, Frédéric GuayABSTRACT (400 words in VII)The aim of this study was to investigate the effect of the piglet growth during the first week of life on ileal expression of genes and on development of the immune system. Eight litters adjusted to 12 piglets were used. Within each litter, the piglet that showed the lowest weight gain (LWG; n = 8) and the one...
Source: Veterinary Immunology and Immunopathology - November 6, 2018 Category: Veterinary Research Source Type: research

Lymphocyte proliferation and apoptosis of lymphocyte subpopulations in bovine leukemia virus-infected dairy cows with high and low proviral load
Publication date: Available online 3 November 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): María Victoria Nieto Farias, Fernando Nogueira de Souza, Pamela Anahí Lendez, Lucía Martínez-Cuesta, Kamila Reis Santos, Alice Maria Melville Paiva Della Libera, María Carolina Ceriani, Guillermina Laura DolciniAbstractBovine leukemia virus (BLV) is one of the most important virus in dairy cattle. The infection behavior follows what we call the iceberg phenomenon: 60% of infected animals do not show clinical signs; 30% develop persistent lymphocytosis (PL); and the remaining 10%, ...
Source: Veterinary Immunology and Immunopathology - November 4, 2018 Category: Veterinary Research Source Type: research

Microbiome modulates intestinal homeostasis against inflammatory diseases
Publication date: Available online 3 November 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Xiaolun Sun, Zhenquan JiaAbstractEliminating prophylactic antibiotics in food animal production has exerted pressure on discovering antimicrobial alternatives (e.g. microbiome) to reduce elevated intestinal diseases. Intestinal tract is a complex ecosystem coupling host cells with microbiota. The microbiota and its metabolic activities and products are collectively called microbiome. Intestinal homeostasis is reached through dynamic and delicate crosstalk between host immunity and microbiome. However, this balance ...
Source: Veterinary Immunology and Immunopathology - November 4, 2018 Category: Veterinary Research Source Type: research

Isolation and characterization of ovine monocyte-derived macrophages from peripheral blood
Publication date: Available online 3 November 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Yongxiao Liu, Dongbing Yang, Shao Wei, Yijuan Nie, Xiaosheng Zhang, Zhengxing Lian, Hongbing HanAbstractAs key elements and targets for various intracellular pathogens, macrophages play an essential role in host defense. Although sheep peripheral blood monocyte-derived cell lines have been established, their phenotypic characteristics and functional properties remain unknown. We have established several sheep macrophage cell lines from peripheral blood adherent cells that can proliferate spontaneously in long-term ...
Source: Veterinary Immunology and Immunopathology - November 4, 2018 Category: Veterinary Research Source Type: research

Compatibility between a rabies vaccine and a combined vaccine against canine distemper, adenovirosis, parvovirosis, parainfluenza virus and leptospirosis
Publication date: Available online 2 November 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): J. Bouvet, C. Cariou, A. Poulard, F. Oberli, L. Cupillard, P.M. GuigalAbstractIn many cicumstances, veterinarians are requiring to be able to administer rabies vaccine in dogs at the same time as vaccinating against canine distemper, adenovirus, parvovirus, parainfluenza virus and leptospirosis. The aim of this study was to assess the compatibility between a multivalent vaccine and a rabies vaccine when injected at two separate sites. Lack of interference was assessed by comparing serological response to viral comp...
Source: Veterinary Immunology and Immunopathology - November 3, 2018 Category: Veterinary Research Source Type: research

Canine primary jejunal and colonic epithelial cells predominantly express TLR5 and TLR9 but do not change TLR expression pattern after stimulation with certain Toll-like receptor ligands
Publication date: Available online 3 November 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Wencke Reineking, Johannes Junginger, Reinhard Mischke, Marion Hewicker-TrautweinAbstractThe intestinal mucosa is in contact with abundant luminal antigens and coordinates immune responses to differentiate commensals from pathogens. Intestinal epithelial cells (IECs) not only represent a physical barrier but also an immunologically important cell type that recognizes microbe-associated molecular patterns via Toll-like receptors (TLR). The importance of TLR expression has been elucidated for intestinal disorders in ...
Source: Veterinary Immunology and Immunopathology - November 3, 2018 Category: Veterinary Research Source Type: research

Fecal microbiota transplantation as a tool to treat and reduce susceptibility to disease in animals
Publication date: Available online 3 November 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Megan C. NiederwerderAbstractFecal microbiota transplantation (FMT) is the process by which fecal microbiota are donated from a healthy individual and subsequently transplanted into a diseased or young individual. The mechanism by which FMT is effective is believed to be due to enhanced beneficial microbes, increased microbiome diversity, and restored normal flora. Beneficial gut microorganisms not only play a role in maintaining an intestinal barrier and metabolizing nutrients, but importantly, these microbes help...
Source: Veterinary Immunology and Immunopathology - November 3, 2018 Category: Veterinary Research Source Type: research

Comparative proteomics identified immune response proteins involved in response to vaccination with heat-inactivated Mycobacterium bovis and mycobacterial challenge in cattle
In conclusion, this study provides a first indication of the role of several immune system pathways in response to the heat-inactivated M. bovis vaccine and mycobacterial challenge. (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - October 30, 2018 Category: Veterinary Research Source Type: research

Interleukin-4 (IL-4) may regulate alternative activation of macrophage-like cells in chickens: a sequential study using novel and specific neutralizing monoclonal antibodies against chicken IL-4
Publication date: Available online 29 October 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Atul A. Chaudhari, Woo H. Kim, Hyun S. LillehojAbstractIn mammals, alternatively activated macrophages (AAMs) are well-recognized and are produced by stimulation with Th2 cytokines such as interleukin 4 (IL-4) and IL-13. On their mammalian counterparts, AAMs in chickens has neither been reported nor the functionality of chicken IL-4 (chIL-4) has been studied till date. Therefore, present study developed mouse monoclonal antibodies (mAbs) against chIL-4 and used these antibodies to investigate whether chIL-4 induces...
Source: Veterinary Immunology and Immunopathology - October 30, 2018 Category: Veterinary Research Source Type: research

Editorial Board
Publication date: October 2018Source: Veterinary Immunology and Immunopathology, Volume 204Author(s): (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - October 26, 2018 Category: Veterinary Research Source Type: research

Endometrial transcripts of proinflammatory cytokine and enzymes in prostaglandin synthesis are upregulated in the bitches with atrophic pyometra
Publication date: Available online 25 October 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Laishram Kipjen Singh, Manas Kumar Patra, Girish Kumar Mishra, Vidya Singh, Vikramaditya Upmanyu, Abhishek C. Saxena, Sanjay Kumar Singh, Goutam Kumar Das, Harendra Kumar, Narayanan KrishnaswamyAbstractInflammatory markers of endometrial origin are valuable in order to differentiate the pyometra from cystic endometrial hyperplasia in the bitch. In the present study, we hypothesized that histological categorization would distinguish the differential regulation of the proinflammatory genes in the endometrium of bitch...
Source: Veterinary Immunology and Immunopathology - October 26, 2018 Category: Veterinary Research Source Type: research

Altered hematological, biochemical and immunological parameters as predictive biomarkers of severity in experimental myocardial infarction
Publication date: Available online 24 October 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Rebeca Blázquez, Verónica Álvarez, Juan Antonio Antequera-Barroso, Claudia báez-Díaz, Virginia Blanco, Juan Maestre, Beatriz Moreno-Lobato, Esther López, Federica Marinaro, Javier G Casado, Verónica Crisóstomo, Francisco Miguel Sánchez-MargalloAbstractPreclinical studies in cardiovascular medicine are necessary to translate basic research to the clinic. The porcine model has been widely used to understand the biological mechanisms involved in cardiovasc...
Source: Veterinary Immunology and Immunopathology - October 25, 2018 Category: Veterinary Research Source Type: research

Co-administration of saponin quil A and PRRSV-1 modified-live virus vaccine up-regulates gene expression of type I interferon-regulated gene, type I and II interferon, and inflammatory cytokines and reduces viremia in response to PRRSV-2 challenge
Publication date: Available online 24 October 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Wasin Charerntantanakul, Saengtong PongjaroenkitAbstractPorcine reproductive and respiratory syndrome virus (PRRSV) is a devastating virus which suppresses the expression of type I and II interferons (IFNs) as well as several pro-inflammatory cytokines. Our previous study reported that saponin quil A had a potential to up-regulate the expression of type I IFN-regulated genes and type I and II IFNs in porcine peripheral blood mononuclear cells (PBMC) inoculated with PRRSV. The present study evaluated the immunostimu...
Source: Veterinary Immunology and Immunopathology - October 25, 2018 Category: Veterinary Research Source Type: research

Clinical-pathological and immunological biomarkers in dogs with atopic dermatitis
This study aimed to investigate hematological, biochemical, clinical, and immunological parameters to contribute to the identification of biomarkers applied to CAD. The results of the analysis on hematologic and clinical parameters showed increased neutrophil numbers and decreased lymphocyte counts. The ex vivo immunophenotyping of leukocytes demonstrated increased counts of circulating neutrophils, in addition to a high frequency of CD4+ T-cells and elevated CD4+/CD8+ T-cell ratio, which were the hallmark of atopic animals. Moreover, atopic dogs presented a mixed immune response, displaying both CD4+ and CD8+ T-cell subse...
Source: Veterinary Immunology and Immunopathology - October 24, 2018 Category: Veterinary Research Source Type: research

Actinobacillus pleuropneumoniae triggers IL-10 expression in tonsils to mediate colonisation and persistence of infection in pigs
Publication date: Available online 22 October 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Andrea Müllebner, Elena L. Sassu, Andrea Ladinig, Janna Frömbling, Ingrid Miller, Monika Ehling-Schulz, Isabel Hennig-Pauka, J. Catharina DuvigneauAbstractActinobacillus pleuropneumoniae(APP) persisting in clinically healthy pigs may be the causative agent of sudden outbreaks of severe respiratory disease in swine herds. During the course of acute disease, the pathogen is eliminated from inflamed lung tissue, which is characterized by the expression of pro-inflammatory cytokines and an influx of neutrophi...
Source: Veterinary Immunology and Immunopathology - October 23, 2018 Category: Veterinary Research Source Type: research

Dissect the mode of action of probiotics in affecting host-microbial interactions and immunity in food producing animals
Publication date: Available online 22 October 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Tao Ma, Yutaka Suzuki, Le Luo GuanAbstractProphylactic antimicrobials have been widely used in food animal production with the aim to prevent infectious diseases, enhance feed efficiency, and promote growth. However, the extensive use of antimicrobials in food animal production systems has led to the emergence of antimicrobial resistant pathogens, which are potential threats to human and animal health. Probiotics have been proposed to be a promising alternative of prophylactic antimicrobials, with potential benefic...
Source: Veterinary Immunology and Immunopathology - October 22, 2018 Category: Veterinary Research Source Type: research

Protein expression in the liver and blood serum in chickens in response to Salmonella Enteritidis infection
In this study, we therefore determined changes in protein abundance in the liver and blood serum in response to S. Enteritidis infection using the unbiased approach of shotgun proteomics. Complement and coagulation cascades, TNF signalling, antigen processing and presentation was activated in the liver following infection with S. Enteritidis. Chicken proteins that decreased in the liver were involved in glycolysis, the citrate cycle, oxidative phosphorylation and fatty acid metabolism. No functional category was significantly activated or suppressed in the serum. Concerning individual proteins, VNN1, SAA, AVD, SERPINA3, SE...
Source: Veterinary Immunology and Immunopathology - October 22, 2018 Category: Veterinary Research Source Type: research

Long-term implantation of acoustic transmitters induces chronic inflammatory cytokine expression in adult rainbow trout (Oncorhynchus mykiss)
Publication date: Available online 15 October 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Shawna L. Semple, Ingeborg M. Mulder, Tania Rodriguez-Ramos, Michael Power, Brian DixonAbstractTelemetry transmitters are frequently used in studies of wild fish migration and behavior. Although the effects of surgically implanted transmitters on survival, tag retention, healing and growth have been studied, there has been little research regarding the potential immune response induced by these transmitters. In the current study, mature rainbow trout received either surgical implantation of an acoustic transmitter ...
Source: Veterinary Immunology and Immunopathology - October 16, 2018 Category: Veterinary Research Source Type: research

Detection of chicken interleukin-10 production in intestinal epithelial cells and necrotic enteritis induced by Clostridium perfringens using capture ELISA
ConclusionCP induced IL-10 production in chicken IECs. Increased IL-10 production was detected in CP-stimulated chicken IECs and in serum collected from CP-infected birds, using antigen capture ELISA. Antigen capture ELISA could be a useful tool to monitor IL-10 production in chicken disease. (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - October 12, 2018 Category: Veterinary Research Source Type: research

The role of the gut microbiome in shaping the immune system of chickens
Publication date: Available online 11 October 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Leon J. Broom, Michael H. KogutAbstractMost animals are colonised by at least as many microbial cells as somatic cells, potentially comprising at least 100 times more genes within just the gut microbiota than the host itself. It is, therefore, evident that such a conglomeration can have a profound effect on various bodily systems, particularly the (gut) immune system. Chickens are major providers of efficiently produced protein for humans but also harbour common foodborne pathogens and are susceptible to significan...
Source: Veterinary Immunology and Immunopathology - October 12, 2018 Category: Veterinary Research Source Type: research

Fetal environment and fetal intestine are sterile during the third trimester of pregnancy
Publication date: Available online 26 September 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Nilusha Malmuthuge, Philip J. GriebelAbstractRecent next generation sequencing studies on host-associated microbiomes generated debatable conclusions regarding the central dogma of fetal gut sterility. These observations challenge the concepts that microbial colonization of the gut begins during and after birth as well as the concept of antigen-independent prenatal maturation of mucosal-associated lymphoid tissue in ruminants and humans. The placental barrier varies markedly among mammalian species with mice and ...
Source: Veterinary Immunology and Immunopathology - October 4, 2018 Category: Veterinary Research Source Type: research

Editorial Board
Publication date: September 2018Source: Veterinary Immunology and Immunopathology, Volume 203Author(s): (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - September 21, 2018 Category: Veterinary Research Source Type: research

Parallel testing increases detection of Mycobacterium bovis-infected African buffaloes (Syncerus caffer)
Publication date: Available online 18 September 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Netanya Bernitz, Wynand J. Goosen, Charlene Clarke, Tanya J. Kerr, Roxanne Higgitt, Eduard O. Roos, David V. Cooper, Robin M. Warren, Paul D. van Helden, Sven D.C. Parsons, Michele A. MillerAbstractThe diagnosis of Mycobacterium bovis (M. bovis) infection in African buffaloes (Syncerus caffer) relies on detection of the cell-mediated immune response to M. bovis antigens using the single comparative intradermal tuberculin test (SCITT) or interferon gamma release assays (IGRAs). The aim of the present study was to ...
Source: Veterinary Immunology and Immunopathology - September 19, 2018 Category: Veterinary Research Source Type: research

Recombinant ferritin-H induces immunosuppression in European sea bass larvae (Dicentrarchus labrax) rather than immunostimulation and protection against a Vibrio anguillarum infection
In this study, we evaluated if the recombinant sea bass ferritin-H could stimulate the innate immune system of gnotobiotic European sea bass larvae resulting in protection against a V. anguillarum challenge. We also evaluated the effect of a V. anguillarum infection on the transcription of immune-related genes in gnotobiotic European sea bass larvae. Recombinant sea bass ferritin-H was produced, encapsulated in calcium alginate microparticles and orally delivered to sea bass larvae at seven days after hatching. Our results showed V. anguillarum caused an acute infection, resulting in high mortality. The infection significa...
Source: Veterinary Immunology and Immunopathology - September 14, 2018 Category: Veterinary Research Source Type: research

C-C motif chemokine ligand (CCL) production in equine peripheral blood mononuclear cells identified by newly generated monoclonal antibodies
Publication date: Available online 11 September 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Christiane L. Schnabel, Michelle Wemette, Susanna Babasyan, Heather Freer, Cynthia Baldwin, Bettina WagnerAbstractChemokines are soluble molecules directing immune cell trafficking and homing, mediating inflammation, and initiating immune responses to infection. In horses, the analysis of chemokines has been limited by the lack of specific antibodies. We generated mAbs specific for the equine C-C motif chemokine ligands (CCL) CCL2 (MCP-1), CCL3 (MIP-1α), CCL5 (RANTES) and CCL11 (eotaxin) using hybridoma tec...
Source: Veterinary Immunology and Immunopathology - September 12, 2018 Category: Veterinary Research Source Type: research

Identification of targets of monoclonal antibodies that inhibit adhesion and growth in Mycoplasma mycoides subspecies mycoides
Publication date: Available online 10 September 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Racheal Aye, Yenehiwot Berhanu Weldearegay, Harrison Osundwa Lutta, Francis Chuma, Andreas Pich, Joerg Jores, Jochen Meens, Jan NaessensAbstractMycoplasma mycoides subspecies mycoides (Mmm) adhesion is tissue and host specific. Inhibition of adhesion will prevent Mmm from binding to lung cells and hence prevent colonization and disease. The aim of this study was to develop a panel of Mmm monoclonal antibodies against Mmm and use these antibodies to investigate their inhibitory effect on the adherence of Mmm to bo...
Source: Veterinary Immunology and Immunopathology - September 11, 2018 Category: Veterinary Research Source Type: research

Antibody-mediated bacterial killing of Ichthyobacterium seriolicida in Japanese amberjack
In this study, fish were passively immunized to determine whether serum antibody is involved in protection against BHJ. The susceptibility of I. seriolicida to the bactericidal activity of Japanese amberjack serum was also investigated. In passive immunization tests, significantly lower mortality was noted in fish that received convalescent serum. Bacteria were killed when exposed to convalescent serum but not serum from naïve fish. Electron microscopic analyses showed that I. seriolicida cells were morphologically altered by reaction with convalescent serum. Naïve fish serum became bactericidal upon addition of ...
Source: Veterinary Immunology and Immunopathology - August 30, 2018 Category: Veterinary Research Source Type: research

Comment on “The domestic cat antibody responseto FHV-1 increases with age”
Publication date: September 2018Source: Veterinary Immunology and Immunopathology, Volume 203Author(s): James A. Roth (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - August 25, 2018 Category: Veterinary Research Source Type: research

Pharmacokinetics of human recombinant C1-esterase inhibitor and development of anti-drug antibodies in healthy dogs
This study aimed to characterize the pharmacokinetics of a single 500 IU IV dose of rC1-INH in 8 healthy beagle dogs, evaluate the dogs for any adverse effects of drug administration, and determine whether rC1-INH administration induces anti-drug antibody formation. Serum rC1-INH concentrations were measured using a commercial functional ELISA at baseline and at 10, 20, 40, 60, 80, 100, 120, 240, 360, 480, 600, 720, 960, and 1440 minutes post drug administration. Complete blood counts were conducted at baseline, 720 and 1440 minutes. Western blot analysis, using rC1-INH as the target antigen was used to detec...
Source: Veterinary Immunology and Immunopathology - August 24, 2018 Category: Veterinary Research Source Type: research

Reply to “The domestic cat antibody response to FHV-1 increases with age”
Publication date: Available online 20 August 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): James A. Roth (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - August 21, 2018 Category: Veterinary Research Source Type: research

Enterotoxic effects of Aeromonas hydrophila infection in the catfish, Clarias gariepinus: Biochemical, histological and proteome analyses
In this study, the impact of A. hydrophila infection in the air-breathing catfish, Clarias gariepinus was analyzed using a multidimensional approach. Aeromonas hydrophila (1 × 107 cells) was injected into C. gariepinus intraperitoneally at an ambient temperature with periodical monitoring for morphological changes. After 7 days post-infection, tissue samples of the gills, liver, intestine, and kidney were subjected to biochemical, histological, transmission electron microscope (TEM) and proteomic analyses. Observed results indicated distinct morphological changes with the significant increase of ROS and...
Source: Veterinary Immunology and Immunopathology - August 20, 2018 Category: Veterinary Research Source Type: research

Detection of anti-neutrophil cytoplasmic antibodies in dogs with immune-mediated inflammatory diseases
Publication date: Available online 16 August 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Takashi Tamamoto, Aki Nagao, Mizuki SuganoAbstractAnti-neutrophil cytoplasmic antibody (ANCA) is a type of autoantibody against neutrophil cytoplasm. In veterinary medicine, few studies have reported the detection of ANCA in dogs, and most of these studies were performed in dogs with inflammatory bowel disease (IBD). The aim of this study was to evaluate whether ANCA is detected in dogs with immune-mediated inflammatory diseases (IMIDs) other than IBD. Serum samples were collected before treatment initiation from 40...
Source: Veterinary Immunology and Immunopathology - August 16, 2018 Category: Veterinary Research Source Type: research

Benzo(a)pyrene suppresses tracheal antimicrobial peptide gene expression in bovine tracheal epithelial cells
Publication date: Available online 16 August 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Laura A. Bourque, Stephen Raverty, Carmon Co, Brandon N. Lillie, Pierre-Yves Daoust, Mary Ellen Clark, Jeff L. CaswellAbstractRespiratory disease is an important cause of morbidity and mortality in cetaceans, which are also threatened by environmental degradation caused by crude oil spills. Following oil spills, cetaceans at the water surface may inhale droplets of oil containing toxic polycyclic aromatic hydrocarbons (PAHs), which could potentially alter respiratory immunity via activation of the aryl hydrocarbon r...
Source: Veterinary Immunology and Immunopathology - August 16, 2018 Category: Veterinary Research Source Type: research

Effects of a pathogenic ETEC strain and a probiotic Enterococcus faecium strain on the inflammasome response in porcine dendritic cells
Publication date: Available online 15 August 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Henriette Loss, Jörg R. Aschenbach, Friederike Ebner, Karsten Tedin, Ulrike LodemannAbstractDendritic cells (DC) are crucial for maintaining intestinal homeostasis and generating proper immune responses to bacteria occurring in the gut. Microbial stimuli can be recognized by intracellular receptors called inflammasomes, e.g., nucleotide oligomerization domain (NOD)-like receptor protein 3 (NLRP3). The aim of the present study was to unravel the inflammasome response of porcine monocyte-derived DC (MoDC). We inv...
Source: Veterinary Immunology and Immunopathology - August 16, 2018 Category: Veterinary Research Source Type: research

The expression of Indoleamine 2, 3-dioxygenase (IDO) is reduced in granulomas from BCG vaccinated cattle compared to granulomas from unvaccinated controls after experimental challenge with Mycobacterium bovis
Publication date: Available online 13 August 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Waldo L. Garcia-Jimenez, Bernardo Villarreal-Ramos, Duncan Grainger, R. Glyn Hewisnon, Hans M. Vordermeier, Francisco J. SalgueroAbstractBovine tuberculosis (bTB), mainly caused by Mycobacterium bovis (M. bovis), is a major economic disease of livestock worldwide. Vaccination is considered as a potentially sustainable adjunct to the current control strategy. Cattle vaccination with the live attenuated M. bovis bacillus Calmette-Guerin (BCG) confers variable protection; the reasons for this variability are not unders...
Source: Veterinary Immunology and Immunopathology - August 14, 2018 Category: Veterinary Research Source Type: research

Enhancing purification and plasma stability of porcine interferon-α/γ by fusion to elastin-like polypeptide
In this study, we evaluated the feasibility of ELP fusion for simple purification and half-life extension of recombinant porcine IFNs (rPoIFNs). After construction of five different fusion expression vectors, we optimized the conditions for soluble protein expression and purification. SDS-PAGE analysis showed that, unlike PoIFNα-His and PoIFNγ-His, PoIFNα-ELP, ELP-PoIFNα and PoIFNαγ-ELP were expressed mainly as soluble proteins at 20 ℃. The optimal conditions for the inverse transition cycling (ITC) of three ELP fusion proteins were 2 M NaCl at 28 ℃. After two rounds of ITC, t...
Source: Veterinary Immunology and Immunopathology - August 14, 2018 Category: Veterinary Research Source Type: research

Blood serum acute phase proteins and iron dynamics during acute phase response of Salmonella enterica Serotype Dublin experimentally infected buffalo calves
Publication date: Available online 4 August 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): André M. Santana, Daniela G. Silva, Funmilola C. Thomas, Priscila A. Bernardes, Lucas J.L. Pizauro, Clarissa H. Santana, Richard J.S. Burchmore, Peter D. Eckersall, José J. FagliariAbstractThe study aimed to evaluate clinical signs, blood serum acute phase proteins (APP) and iron dynamics during the acute phase response (APR) of Salmonella Dublin experimentally infected Murrah buffalo calves. Six buffalo calves constituted the control group (CNT) and six were orally inoculate with 108 CFU of S. Dublin (...
Source: Veterinary Immunology and Immunopathology - August 4, 2018 Category: Veterinary Research Source Type: research

Editorial Board
Publication date: August 2018Source: Veterinary Immunology and Immunopathology, Volume 202Author(s): (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - August 4, 2018 Category: Veterinary Research Source Type: research

Resveratrol administration increases phagocytosis, decreases oxidative burst, and promotes pro-inflammatory cytokine production in healthy dogs
The objective of this study was to determine the short-term effect of resveratrol on the healthy canine innate immune system in vivo. The hypothesis was that resveratrol would spare phagocytosis, depress the vigor of PMN oxidative burst, and result in a proinflammatory stimulated leukocyte cytokine profile in vivo. In an open-label study, whole blood was collected from 12 healthy, adult client-owned dogs on day 0 and 3. Six dogs received resveratrol, 200 mg kg-1, orally once daily for three days and six dogs served as controls with no supplement administered. Phagocytosis, oxidative burst and pathogen associa...
Source: Veterinary Immunology and Immunopathology - July 31, 2018 Category: Veterinary Research Source Type: research

Molecular cloning and expression analyses of immunoglobulin tau heavy chain (IgT) in turbot, Scophthalmus maximus
Publication date: Available online 30 July 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Xiaoqian Tang, Yang Du, Xiuzhen Sheng, Jing Xing, Wenbin ZhanAbstractImmunoglobulins (Igs) are humoral mediators playing the prevailing role in the innate and adaptive immunities of jawed vertebrates and provide obligatory duties to protect the organism from a wide variety of pathogens. In the present study, the membrane-bound and secretory immunoglobulin T (mIgT and sIgT) genes of turbot (Scophthalmus maximus) were cloned for the first time with the intention of better understanding the IgT functions. The mIgT cDNA i...
Source: Veterinary Immunology and Immunopathology - July 30, 2018 Category: Veterinary Research Source Type: research

Lack of cross-reactivity of human and porcine reagents to quantify manatee (Trichechus manatus) cytokines
Publication date: Available online 29 July 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Milton Levin, Lindsay Jasperse, Erika Gebhard, Estelle Rousselet, Catherine WalshAbstractVeterinary medical examinations, including both physical examination and diagnostic tests, are important to monitor the health of both managed-care and wild marine mammals. However, limited species-specific reagents and assays are available that may contribute to a broader medical examination. This project evaluated if commercially available human and porcine antibodies and reagents would cross-react with manatee (Trichechus manat...
Source: Veterinary Immunology and Immunopathology - July 30, 2018 Category: Veterinary Research Source Type: research

Use of ELISA and Western blot for serological detection of antibodies to E-S antigens of Trichinella spiralis muscle larvae in sera of swine experimentally infected with Trichinella spiralis
Publication date: Available online 29 July 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Michał Gondek, Justyna Bień, Zygmunt NowakowskiABSTRACTThe aim of the present study was to evaluate the detection of trichinellosis using ELISA, and to determine the degree of variation of IgG antibodies against excretory-secretory (ES) antigens of T. spiralis muscle larvae. Ten young Polish Large White native breed pigs were experimentally inoculated with a low dose of 300 invasive Trichinella spiralis (T. spiralis) muscle larvae (ML). Pig sera were collected at 7 and 9 days prior to the experimental infection with...
Source: Veterinary Immunology and Immunopathology - July 30, 2018 Category: Veterinary Research Source Type: research

Sheep and cattle exposed to Mycobacterium avium subspecies paratuberculosis exhibit altered total serum cholesterol profiles during the early stages of infection
This study sought to evaluate the levels of total serum cholesterol in the host following exposure to MAP. Blood samples were collected from both sheep and cattle prior to experimental challenge with MAP and at monthly intervals post-challenge. Total serum cholesterol levels in sheep challenged with MAP were significantly elevated at 9 weeks post-inoculation (wpi) in comparison to controls. When stratified based on disease outcome, there was no significant difference in serum cholesterol at the timepoints examined between MAP exposed sheep that were susceptible and those that were resistant to Johne’s disease. There ...
Source: Veterinary Immunology and Immunopathology - July 21, 2018 Category: Veterinary Research Source Type: research

Effect of antigen challenge on dynamics of cd62p and cd41/61 expression on platelets in horses with recurrent airway obstruction (rao)
Publication date: Available online 18 July 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Alicja Iwaszko, Hieronim Borowicz, Stanislaw Graczyk, Malwina Slowikowska, Aleksandra Pliszczak-Krol, Artur NiedzwiedzAbstractRecurrent airway obstruction (RAO), also known as heaves, is an allergic respiratory condition that develops in horses following an exposure to aeroallergens in hay and straw. This is manifested by airway hyperreactivity, inflammation, bronchoconstriction, as well as a leukocyte and platelet infiltration into the airways. Platelet activation and an increase in circulating platelet-leukocyte agg...
Source: Veterinary Immunology and Immunopathology - July 19, 2018 Category: Veterinary Research Source Type: research