Characterization of CD79αcy+ cells in placentas from ruminants
Publication date: Available online 13 September 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): Germán J. Cantón, Alex Schock, Noelita Melo de Sousa, Jean-François Beckers, Francesca ChianiniAbstractPrevious work carried out to characterise different immune cells in ruminant placentas found strong CD79αcy nuclear labelling in cells histologically resembling trophoblast cells. In the attempt to characterize this cell population, placentomes collected from cattle, sheep and water buffaloes were examined by immunohistochemistry with single and double labelling using monoclonal antibo...
Source: Veterinary Immunology and Immunopathology - September 14, 2019 Category: Veterinary Research Source Type: research

Assessing the clinical and bacteriological outcomes of vaccination with recombinant Asp14 and OmpA against A. phagocytophilum in sheep
Publication date: Available online 11 September 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): Sveinung Eskeland, Snorre Stuen, Francy L. Crosby, Kari Lybeck, Anthony F. Barbet, Per-Eric Lindgren, Stig Tollefsen, Peter Wilhelmsson, Tore S. Tollersrud, Shokouh Makvandi-Nejad, Erik G. GranquistAbstractAnaplasma phagocytophilum is a tick borne bacterium, causing disease in sheep and other mammals, including humans. The bacterium has great economic and animal welfare implications for sheep husbandry in Northern Europe. With the prospect of a warmer and more humid climate, the vector availability will likely in...
Source: Veterinary Immunology and Immunopathology - September 12, 2019 Category: Veterinary Research Source Type: research

The failure of a DNA prime/protein boost regime and CTLA-4 mediated targeting to improve the potency of a DNA vaccine encoding Fasciola hepatica phosphoglycerate kinase in sheep
Publication date: Available online 9 September 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): Wesołowska Agnieszka, Basałaj Katarzyna, Zawistowska-Deniziak Anna, Januszkiewicz Kamil, Kozak Ljunggren Monika, Jedlina Luiza, Wędrychowicz HalinaAbstractDNA vaccination in large animals has often been associated with poor immunogenicity, consequently several approaches have been evaluated to enhance its efficacy. Here, we tested a cDNA encoding a phosphoglycerate kinase from Fasciola hepatica (cDNA-FhPGK/pCMV) as a vaccine against ovine fasciolosis and investigated whether a DNA prime/protein boost regime or ...
Source: Veterinary Immunology and Immunopathology - September 10, 2019 Category: Veterinary Research Source Type: research

Detection of MERS-CoV antigen on formalin-fixed paraffin-embedded nasal tissue of alpacas by immunohistochemistry using human monoclonal antibodies directed against different epitopes of the spike protein
Publication date: Available online 9 September 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): Ann-Kathrin Haverkamp, Berend J. Bosch, Ingo Spitzbarth, Annika Lehmbecker, Nigeer Te, Albert Bensaid, Joaquim Segalés, Wolfgang BaumgärtnerAbstractMiddle East respiratory syndrome (MERS) represents an important respiratory disease accompanied by lethal outcome in one third of human patients. In recent years, several investigators developed protective antibodies which could be used as prophylaxis in prospective human epidemics. In the current study, eight human monoclonal antibodies (mAbs) with neutral...
Source: Veterinary Immunology and Immunopathology - September 10, 2019 Category: Veterinary Research Source Type: research

Canine multicentric lymphoma exhibits systemic and intratumoral cytokine dysregulation
Publication date: Available online 9 September 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): Joana N.R. Dias, Mariana Lopes, Conceição Peleteiro, Gonçalo Vicente, Telmo Nunes, Luísa Mateus, Frederico Aires-da-Silva, Luís Tavares, Solange GilAbstractNon-Hodgkin Lymphoma (NHL) is among the most common neoplasias in dogs and humans. Owing to remarkable similarities with its human counterpart, the canine lymphoma (cNHL) model has been proposed as a powerful framework for rapid and clinically relevant translation of novel immunotherapies. However, the establishment of cNHL as...
Source: Veterinary Immunology and Immunopathology - September 10, 2019 Category: Veterinary Research Source Type: research

Immunological response to porcine reproductive and respiratory syndrome virus in young pigs obtained from a PRRSV-positive exposure status herd in a PRRSV endemic area
Publication date: Available online 6 September 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): Nattinee Kittiwan, Panuwat Yamsakul, Pakpoom Tadee, Phacharaporn Tadee, Aniroot Nuangmek, Phongsakorn Chuammitri, Prapas PatchaneeAbstractPorcine reproductive and respiratory syndrome (PRRS), caused by the PRRS virus (PRRSV), remains a major economic threat to swine production throughout the world. The aim of this study was to investigate the humoral and cell-mediated immune responses to PRRSV in 10 PRRSV vaccinated and 10 non-vaccinated young pigs obtained from a PRRSV-seropositive herd under field conditions. On...
Source: Veterinary Immunology and Immunopathology - September 7, 2019 Category: Veterinary Research Source Type: research

Distribution of regulatory T cells in inflammatory colorectal polyps of Miniature Dachshunds
Publication date: Available online 3 September 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): Keisuke Konishi, Hirotaka Igarashi, Shingo Maeda, Eri Uchida, Kiwamu Hanazono, Takashi Tamamoto, Kazuyuki Uchida, Daiji Endoh, Koichi OhnoAbstractInflammatory colorectal polyp (ICRP) is an emerging disease in Miniature Dachshunds (MDs). Animals with this disease exhibit multiple polyps with severe neutrophil infiltration that respond to immunosuppressive therapy. Macrophages in polypoid lesions have been described to play an important role in neutrophil infiltration in the lesion by producing IL-8. In contrast, IL...
Source: Veterinary Immunology and Immunopathology - September 4, 2019 Category: Veterinary Research Source Type: research

Microalgae supplementation to late gestation sows and its effects on the health status of weaned piglets fed diets containing high- or low-quality protein sources
Publication date: Available online 3 September 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): A.V. Lee, L. You, S.Y. Oh, Z. Li, R.E. Fisher-Heffernan, T.R.H. Regnault, C.F.M. de Lange, L. Huber, N.A. KarrowAbstractMaternal stress, such as a bacterial infection occurring in late gestation, may predispose offspring to a variety of diseases later in life. It may also alter programming of developing systems within the fetus, such as the hypothalamic-pituitary-adrenal (HPA) axis and immune system. Dietary supplementation during the last trimester of pregnancy with immune-modulating compounds may be a means of r...
Source: Veterinary Immunology and Immunopathology - September 4, 2019 Category: Veterinary Research Source Type: research

Investigating the role of interleukin 10 on Eimeria intestinal pathogenesis in broiler chickens
Publication date: Available online 31 August 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): Maria Arendt, Jonathan Elissa, Natalie Schmidt, Emily Michael, Nicole Potter, Mark Cook, Laura J. KnollAbstractEimeria species are intestinal protozoan parasites that cause lack of production, malabsorption and mortality in floor raised chickens. Administering an oral antibody to interleukin 10 (aIL-10) reduces the symptoms of coccidiosis in broilers, indicating interleukin 10 (IL-10) is key to Eimeria pathology. IL-10 is an anti-inflammatory cytokine and acts as a stand down signal to reduce inflammation and host p...
Source: Veterinary Immunology and Immunopathology - September 1, 2019 Category: Veterinary Research Source Type: research

Prevalence and risk factors for the presence of serum antibodies against canine distemper, canine parvovirus, and canine adenovirus in communities in mainland Ecuador
Publication date: Available online 28 August 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): Brian A. DiGangi, Patricia A. Dingman, C. Jaime Grijalva, Mark Belyeu, Sylvia Tucker, Ramiro IsazaAbstractThe purpose of this study was to estimate the apparent prevalence and identify risk factors for antibody levels (AL) against canine distemper virus (CDV), canine parvovirus (CPV), and canine adenovirus (CAV) in three communities in the metropolitan area of Quito, Ecuador that have limited access to regular veterinary care. Whole blood samples were collected from 154 dogs presenting to three veterinary field clin...
Source: Veterinary Immunology and Immunopathology - August 28, 2019 Category: Veterinary Research Source Type: research

Ambient ammonia does not appear to inhibit the immune response to infectious bronchitis virus vaccination and protection from homologous challenge in broiler chickens
This study examined the effect of NH3 on the immune response to infectious bronchitis virus (IBV) vaccination and protection against homologous serotype challenge in commercial broiler chickens. One-day-old chicks were administered IBV vaccine and exposed to 30-60 ppm of NH3. At 28 DOA, birds were challenged oculonasally with a pathogenic homologous IBV, and protection was measured by viral detection, clinical signs, ciliostasis, and presence of airsacculitis. IBV-specific serum IgG and lacrimal fluid IgA titers, as well as Harderian gland (HG) immune cell phenotypes, were evaluated. Ammonia exposure was associated ...
Source: Veterinary Immunology and Immunopathology - August 24, 2019 Category: Veterinary Research Source Type: research

An interferon-gamma release assay for the diagnosis of the Mycobacterium bovis infection in white rhinoceros (Ceratotherium simum)
Publication date: Available online 15 August 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): Josephine Chileshe, Eduard O. Roos, Wynand J. Goosen, Peter Buss, Guy Hausler, Leana Rossouw, Tebogo Manemela, Paul van Helden, Robin Warren, Sven DC Parsons, Michele MillerAbstractMycobacterium bovis (M. bovis), the cause of bovine tuberculosis, is endemic in Kruger National Park (KNP), South Africa. The risk of spread of M. bovis infection currently prevents translocation of white rhinoceros (Ceratotherium simum) from this population. Therefore, accurate assays are necessary for screening this threatened species. ...
Source: Veterinary Immunology and Immunopathology - August 16, 2019 Category: Veterinary Research Source Type: research

Potential of the dual IFN-γ/IL-2 fluorescence-immunospot assay to distinguish different stages in bovine tuberculosis
In conclusion, our FluoroSpot is a valid method to enumerate individual antigen-specific IFN-γ+ and IL-2+ T cell subsets ex vivo. The greater levels of single IL-2 producing T cells associated with the presence of pathology could be a potential biomarker for active TB in cattle. (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - August 15, 2019 Category: Veterinary Research Source Type: research

Editorial Board
Publication date: September 2019Source: Veterinary Immunology and Immunopathology, Volume 215Author(s): (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - August 13, 2019 Category: Veterinary Research Source Type: research

Effect of FMD vaccination schedule of dams on the level and duration of maternally derived antibodies
Publication date: Available online 11 August 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): B. Sareyyüpoğlu, V. Gülyaz, C. Çokçalışkan, Y. Ünal, T. Çökülgen, E. Uzunlu, S. Gürcan, O. İlkAbstractVaccination against Foot and Mouth Disease (FMD) in pregnant cows is crucial to produce greater immunity in new born calves, especially in late gestation, as this directly affects neonatal immunity. Therefore, we aimed to investigate how late gestation FMD vaccination of pregnant cows affects the maternally derived antibodies in their offspring. Pregnant cows we...
Source: Veterinary Immunology and Immunopathology - August 12, 2019 Category: Veterinary Research Source Type: research

Effect of Mycoplasma bovis on expression of inflammatory cytokines and matrix metalloproteinases mRNA in bovine synovial cells
In this study, we examined the immunologic response of bovine synovial tissue to M. bovis. We observed significant increases in expression of interleukin (IL)-1β, IL-6, IL-8, MMP-1, and MMP-3 mRNA in synovial tissue from Mycoplasma arthritis calves compared with tissues from normal calves. Expression of IL-6, IL-8, and MMP-1 mRNA was also induced in cultured synovial cells stimulated with M. bovis, but not expression of IL-1β and MMP-3 mRNA. In contrast, the culture supernatant of peripheral blood mononuclear cells stimulated with M. bovis induced marked increases in the expression of IL-1β, IL-6, IL-8, MMP-...
Source: Veterinary Immunology and Immunopathology - August 9, 2019 Category: Veterinary Research Source Type: research

Genetic polymorphisms in Toll-like receptors 1, 2, and 4 in feline upper respiratory tract aspergillosis
Publication date: Available online 7 August 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): J. Whitney, B. Haase, J. Beatty, V.R. BarrsAbstractFungal species in the genus Aspergillus are environmental saprophytes that can act as opportunistic pathogens of the nasal cavity and paranasal sinuses in humans, cats and other species. Upper respiratory tract aspergillosis (URTA) presents as non-invasive and invasive forms with the latter occurring almost exclusively in immunocompromised hosts. However, in domestic cats, invasive URTA affects apparently immunocompetent patients. A defect in innate immunity has been...
Source: Veterinary Immunology and Immunopathology - August 9, 2019 Category: Veterinary Research Source Type: research

Development and validation of an ELISA for the quantification of bovine ITIH4 in serum and milk
In this study we developed an ELISA method which allows the quantification of bovine ITIH4 in serum and milk samples. Previously developed antibodies were used to perform the assay, including anti bovine ITIH4 polyclonal antibodies and a monoclonal antibody against pig ITIH4 that also recognizes the bovine homologous protein. The ELISA developed showed an adequate precision, with inter and intra- assay coefficients of variation lower than 10 % for serum and milk samples. The assay keeps linearity under dilution for both serum and milk samples. A good agreement was observed between the values measured by ELISA and radial im...
Source: Veterinary Immunology and Immunopathology - August 9, 2019 Category: Veterinary Research Source Type: research

Impact of Mycobacterium bovis-induced pathology on interpretation of QuantiFERON®-TB Gold assay results in African buffaloes (Syncerus caffer)
In this study, IP-10 and interferon gamma (IFN-γ) concentrations in whole blood samples from M. bovis culture-confirmed buffaloes with varying degrees of pathological changes (n = 72) and uninfected controls (n = 70) were measured in the IP-10 release assay (IPRA) and IFN-γ release assay (IGRA), respectively. Findings suggest that concentrations of both cytokines in QFT Nil tubes were higher in infected buffaloes with macroscopic pathological changes consistent with bovine tuberculosis compared to uninfected controls, and IGRA values increased with more severe pathological changes in...
Source: Veterinary Immunology and Immunopathology - August 9, 2019 Category: Veterinary Research Source Type: research

WC1+ and WC1neg γδ T lymphocytes in intestinal mucosa of healthy and Mycobacterium avium subspecies paratuberculosis-infected calves
Publication date: Available online 6 August 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): Latasha Ludwig, Rebecca Egan, Monica Baquero, Amanda Mansz, Brandon L. PlattnerAbstractMucosal surfaces such as the gastrointestinal tract, and skin are the front line of host defence and immunity against many pathogens. Gamma delta (γδ) T lymphocytes preferentially localize to the mucosal surfaces in several species including cattle, and are thought to play crucial roles in immunosurveillance and host defence, particularly against mycobacteria. Many γδ T cells are present in young calves, whi...
Source: Veterinary Immunology and Immunopathology - August 7, 2019 Category: Veterinary Research Source Type: research

Characterization of myeloid-derived suppressor cells and cytokines GM-CSF, IL-10 and MCP-1 in dogs with malignant melanoma receiving a GD3-based immunotherapy
The objective of this study was to characterize major MDSCs subsets (monocytic and polymorphonuclear) and the cytokines granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin 10 (IL-10) and monocyte chemoattractant protein-1 (MCP-1) in canines with malignant melanoma and to evaluate changes in MDSCs and the cytokines over time in response to a GD3-based active immunotherapy. Whole blood and serum collected from 30 healthy controls and 33 patients enrolled in the University of Florida melanoma vaccine trial were analyzed by flow cytometry with canine specific CD11b, MHCII and anti-human CD14 antibodies to as...
Source: Veterinary Immunology and Immunopathology - August 2, 2019 Category: Veterinary Research Source Type: research

Editorial Board
Publication date: August 2019Source: Veterinary Immunology and Immunopathology, Volume 214Author(s): (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - August 2, 2019 Category: Veterinary Research Source Type: research

Generation of porcine monoclonal antibodies based on single cell technologies
In this study, we established a platform for producing porcine mAbs based on single cell technologies. First, we singled out an optimal donor from 507 pigs based on serum antibody neutralizing activity against porcine reproductive and respiratory syndrome virus (PRRSV). After identifying the contribution of IgG to the neutralizing activity, single CD45R+IgG+Ag+ B cells were sorted from peripheral blood mononuclear cells (PBMCs). Single B cell RT-PCR was performed using primers designed to cover the germline repertoire of the porcine VH/VL gene segments. Paired VH/VLs were cloned into a eukaryotic expression vector and tran...
Source: Veterinary Immunology and Immunopathology - July 31, 2019 Category: Veterinary Research Source Type: research

Effect of bovine genotype on innate immune response of heifers to repeated lipopolysaccharide (LPS) administration
Publication date: Available online 31 July 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): Georgina Cousillas Boam, Wanda J. Weber, Aimee Benjamin, Stanislaw Kahl, G. Allen Bridges, Theodore H. Elsasser, David Kerr, Brian A. CrookerAbstractThis pilot study provides a preliminary assessment of the impact of genotype on acute innate immune pro-inflammatory, metabolic and endocrine responses to repeated lipopolysaccharide (LPS) administered to growing heifers. Heifers (n = 4/genotype) were from unselected (stable milk yield since 1964, UH) or contemporary (CH) Holstein cows that differed in milk ...
Source: Veterinary Immunology and Immunopathology - July 31, 2019 Category: Veterinary Research Source Type: research

Minipigs as a neonatal animal model for tuberculosis vaccine efficacy testing
Publication date: Available online 26 July 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): Laylaa Ramos, Andres Obregon-Henao, Marcela Henao-Tamayo, Richard Bowen, Angelo Izzo, Joan K. Lunney, Mercedes Gonzalez-JuarreroAbstractMany vaccines against childhood diseases are administered early after birth, but vaccine development studies frequently test efficacy in adult rather than in neonatal animal models. In countries with endemic tuberculosis (TB), Bacillus Calmette-Guerin (BCG) is administered as part of the neonatal vaccine regimen because it prevents against the disseminated form of TB in children, alth...
Source: Veterinary Immunology and Immunopathology - July 27, 2019 Category: Veterinary Research Source Type: research

Development, analytical validation, and initial clinical evaluation of a radioimmunoassay for the measurement of soluble CD25 concentrations in canine serum
Publication date: Available online 27 July 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): A. Buono, J.A. Lidbury, C. Wood, H. Wilson-Robles, L.J. Dangott, K. Allenspach, J.S. Suchodolski, J.M. SteinerAbstractDuring immune activation, CD25 is expressed by T cells, and its soluble form (sCD25) is released into the extracellular matrix and the bloodstream. In humans, serum sCD25 concentrations are used as a surrogate marker for autoimmune diseases, malignancies, and transplant rejection. However, a canine-specific assay for the measurement of sCD25 in dog serum has not previously been described. Therefore, th...
Source: Veterinary Immunology and Immunopathology - July 27, 2019 Category: Veterinary Research Source Type: research

Improved clonality analysis based on immunoglobulin kappa locus for canine cutaneous plasmacytoma
In conclusion, clonality testing based on IGH and IGK may be beneficial as an adjunct tool for diagnosis of canine B cell tumors including cutaneous plasmacytoma. (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - July 25, 2019 Category: Veterinary Research Source Type: research

High incidence of anti-cytokine autoantibodies in dogs with immune diseases suggests important immuno-regulatory functions
Publication date: Available online 15 July 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): M. Autran-Martínez, P. Cortes-Acevedo, L. Cerón-Gutiérrez, S. Davies, Elvira S. Mendoza, H.A. Martínez-Rodríguez, C. Liboni, V. Pérez-Valencia, R. Döffinger, G. Barcenas-MoralesAbstractAutoantibodies against cytokines have been associated with immunodeficiency, susceptibility to infectious diseases, autoimmunity and inflammation in humans, but have not yet been investigated in the Veterinary field so far. The aim of the current study was to determine the presence of anti-...
Source: Veterinary Immunology and Immunopathology - July 17, 2019 Category: Veterinary Research Source Type: research

Editorial Board
Publication date: July 2019Source: Veterinary Immunology and Immunopathology, Volume 213Author(s): (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - July 14, 2019 Category: Veterinary Research Source Type: research

Publisher's Note
Publication date: July 2019Source: Veterinary Immunology and Immunopathology, Volume 213Author(s): (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - July 14, 2019 Category: Veterinary Research Source Type: research

Effects of cyclosporine and dexamethasone on canine T cell expression of interleukin-2 and interferon-gamma
Publication date: Available online 11 July 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): Claire L. Fellman, Todd M. Archer, Robert W. Wills, Andrew J. MackinAbstractCyclosporine and glucocorticoids are powerful immunosuppressive agents used to treat many inflammatory diseases in dogs. Cyclosporine inhibits calcineurin-dependent pathways of T cell activation and resultant T cell cytokine production, and glucocorticoids directly inhibit genes coding for cytokines. Little work has been done comparing the effects of these agents on T cell cytokine production in dogs. Our study measured T cell interleukin-2 (I...
Source: Veterinary Immunology and Immunopathology - July 12, 2019 Category: Veterinary Research Source Type: research

Clonality testing as complementary tool in the assessment of different patient groups with canine chronic enteropathy
Publication date: Available online 10 July 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): Nicole Luckschander-Zeller, Sabine E. Hammer, Barbara C. Ruetgen, Alexander Tichy, Johann G. Thalhammer, E. Haas, Barbara Richter, Monika Welle, Iwan A. BurgenerAbstractDifferentiation between canine chronic enteropathy (CCE) and intestinal lymphoma is a diagnostic challenge as histopathology might fail to yield unequivocal results. Detection of clonal rearrangements of the T-cell-receptor gamma (TCRG) chain and IG heavy chain (IGH) V-J genes offer a useful solution. In this retrospective study, histopathology samples...
Source: Veterinary Immunology and Immunopathology - July 12, 2019 Category: Veterinary Research Source Type: research

Immunological Responses and Evaluation of the Protection in Dairy Cows Vaccinated with Staphylococcal Surface Proteins
The objective of this study was to evaluate the immune responses and protection in dairy cows vaccinated with S. aureus surface proteins (SASP) or S. chromogenes surface proteins (SCSP). We divided eighteen Holstein dairy cows randomly into three groups of 6 animals each. We vaccinated group 1 and 2 animals with SASP and SCSP with Emulsigen-D adjuvant, respectively. We injected control (group 3) animals with PBS (pH 7.2) in Emulsigen®-D. We vaccinated animals three times at 28 and 14 days before drying off, and at dry off. Two weeks after the third vaccination, we challenged each animal by dipping all teats in S. aureu...
Source: Veterinary Immunology and Immunopathology - July 6, 2019 Category: Veterinary Research Source Type: research

Tumor microenvironment and stroma in intestinal adenocarcinomas and associated metastases in Atlantic salmon broodfish (Salmo salar)
Publication date: Available online 4 July 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): Håvard Bjørgen, Hege Hellberg, Oskar Mongstad Løken, Gjermund Gunnes, Erling Olaf Koppang, Ole Bendik DaleAbstractAnimal models are invaluable tools in cancer research. In this context, salmon is a promising candidate. Intestinal adenocarcinoma with metastases may be induced as a consequence of a plant-based diet triggering the inflammation – dysplasia- carcinogenesis pathway. Here, we investigate the stroma and the presence and nature of immune cells in such tumors by staining for mast cells,...
Source: Veterinary Immunology and Immunopathology - July 6, 2019 Category: Veterinary Research Source Type: research

Elimination of erroneous results related to bovine mononuclear cell immunophenotyping by antibodies binding to Fc receptors
Publication date: Available online 2 July 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): Cintia Hiromi Okino, Rodrigo Giglioti, César Cristiano Bassetto, Pamella Cristini Silva, Henrique Nunes de Oliveira, Márcia Cristina de Sena OliveiraAbstractBlocking immunoglobulin G (IgG) binding receptors on leukocytes is an established and highly recommended preventive procedure for immunological assays. Failing to prevent such nonspecific binding can lead to erroneous results. Several studies testing different blocking reagents have been performed in murine or human cells, however, there are no specif...
Source: Veterinary Immunology and Immunopathology - July 2, 2019 Category: Veterinary Research Source Type: research

Felis catus papillomavirus type 2 virus-like particle vaccine is safe and immunogenic but does not reduce FcaPV-2 viral loads in adult cats
Publication date: Available online 28 June 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): Neroli A. Thomson, Laryssa Howe, Karin Weidgraaf, David G. Thomas, Vivienne Young, Vernon K. Ward, John S. MundayAbstractFelis catus papillomavirus type 2 (FcaPV-2) commonly infects the skin of domestic cats and has been associated with the development of skin cancer. In the present study, a FcaPV-2 virus-like particle (VLP) vaccine was produced and assessed for vaccine safety, immunogenicity, and impact on FcaPV-2 viral load. This is the first report of the use of a papillomavirus VLP vaccine in domestic cats. The Fc...
Source: Veterinary Immunology and Immunopathology - June 29, 2019 Category: Veterinary Research Source Type: research

Corrigendum to “A single, low dose oral antigen exposure in newborn piglets primes mucosal immunity if administered with CpG oligodeoxynucleotides and polyphosphazene adjuvants” [Vet. Immunol. Immunopathol. 161 (2014) 211–221]
Publication date: Available online 27 June 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): J. Alex Pasternak, Siew Hon Ng, Heather L. Wilson (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - June 27, 2019 Category: Veterinary Research Source Type: research

Effect of progesterone on the vaccination and immune response against Chlamydia abortus in sheep
Publication date: Available online 27 June 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): A. Murcia-Belmonte, D. Álvarez, N. Ortega, J.A. Navarro, E. Gómez-Lucía, A.J. Buendía, J. Sánchez, L. del Río, J. Salinas, M.R. CaroAbstractChlamydia abortus produces ovine enzootic abortion (OEA). Symptoms are not observed until the organism colonises the placenta, eventually causing abortion. Infected animals become carriers and will shed the organism in the following oestruses. This process suggests that sex hormones might play an important role in the physiopathology of OE...
Source: Veterinary Immunology and Immunopathology - June 27, 2019 Category: Veterinary Research Source Type: research

Protec™ improves innate immune response and specific antibody response against Lactococcus garvieae in rainbow trout (Oncorhynchus mykiss)
Publication date: July 2019Source: Veterinary Immunology and Immunopathology, Volume 213Author(s): Chiara Bulfon, Valentina Pacorig, Massimo Sarti, Umberto Luzzana, Marco Galeotti, Donatella VolpattiAbstractProtec™ is a commercial aquafeed (Skretting Italia) containing a combination of glucans, vitamin C, vitamin E and zinc (immune support pack). No research information concerning its capability to improve fish immune response is available, so in this study the potential immunomodulatory effects of Protec™ were investigated in rainbow trout (Oncorhynchus mykiss). Head kidney (HK) leukocytes from adult fish (100...
Source: Veterinary Immunology and Immunopathology - June 27, 2019 Category: Veterinary Research Source Type: research

Early IgG2 in calves experimentally infected with Mycobacterium avium subsp. paratuberculosis
In this study, an experimental Map-infection model of calves was used to improve the knowledge of early antibody response and to evaluate different in-house ELISAs in the detection of subclinical paratuberculosis. Calves were challenged with Map strain IS900-RFLPA (n = 3) or Map strain IS900-RFLPC (n = 2) (Argentinean isolated strains) or mock infected (n = 3), and their specific humoral response was evaluated. The diagnostic ELISA (IgG against Map protoplasmic antigen; PPA) could not detect the infection throughout the experimental period (180 days post-infection; dpi), whereas th...
Source: Veterinary Immunology and Immunopathology - June 26, 2019 Category: Veterinary Research Source Type: research

ProtecTM improves innate immune response and specific antibody response against Lactococcus garvieae in rainbow trout (Oncorhynchus mykiss)
Publication date: Available online 20 June 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): Chiara Bulfon, Valentina Pacorig, Massimo Sarti, Umberto Luzzana, Marco Galeotti, Donatella VolpattiAbstractProtecTM is a commercial aquafeed (Skretting) containing a combination of glucans, vitamin C, vitamin E and zinc (immune support pack). No research information concerning its capability to improve fish immune response is available, so in this study the potential immunomodulatory effects of ProtecTM were investigated in rainbow trout (Oncorhynchus mykiss). Head kidney (HK) leukocytes from adult fish (100 g...
Source: Veterinary Immunology and Immunopathology - June 21, 2019 Category: Veterinary Research Source Type: research

RNA-seq analysis of viral gene expression in the skin of Marek’s disease virus infected chickens
Publication date: Available online 18 June 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): Lakshmi Sunkaraa, Syed Mudasir Ahmad, Mohammad HeidariAbstractMarek’s disease virus (MDV), a highly cell-associated oncogenic avian α-herpesvirus, is the causative agent of malignant transformation of T cells in domestic chickens. The latently infected CD4+CD8- T cells carry the virus through the blood stream and establish lymphomas in the skin, visceral organs and peripheral nerves. The feather follicle epithelium (FFE) is the only anatomical site where fully infectious enveloped virions are produced and ...
Source: Veterinary Immunology and Immunopathology - June 18, 2019 Category: Veterinary Research Source Type: research

Editorial Board
Publication date: June 2019Source: Veterinary Immunology and Immunopathology, Volume 212Author(s): (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - June 17, 2019 Category: Veterinary Research Source Type: research

Changes in the intestinal mucosal proteome of turkeys (Meleagris gallopavo) infected with haemorrhagic enteritis virus
Publication date: Available online 7 June 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): Andreia Tomás Marques, Sandra I. Anjo, Mangesh Bhide, Ana Varela Coelho, Bruno Manadas, Cristina Lecchi, Guido Grilli, Fabrizio CecilianiAbstractHaemorrhagic enteritis (HE) is a viral disease affecting intestinal integrity and barrier function in turkey (Meleagris gallopavo) and resulting in a significant economic loss. Sequential Windowed Acquisition of All Theoretical Fragment Ion Mass Spectra (SWATH-MS) was applied to identify crucial proteins involved in HE infection. A total of 938 proteins were identified ...
Source: Veterinary Immunology and Immunopathology - June 8, 2019 Category: Veterinary Research Source Type: research

Food allergen-specific sublingual immunotherapy modulates peripheral T cell responses of dogs with adverse food reactions
Publication date: Available online 29 May 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): Elisa Maina, Bert Devriendt, Eric CoxAbstractFood allergen-specific sublingual immunotherapy (FA-SLIT) is a novel, safe and effective approach in dogs with adverse food reactions (AFR) to reduce their clinical symptoms. However, little is known about the specific immune components which mediate this reduction in clinical symptoms. In humans, regulatory T cells seem to play an important role in this desensitisation process. Here, we investigated changes in peripheral T cell responses of dogs with AFR upon FA-SLIT. Five ...
Source: Veterinary Immunology and Immunopathology - May 30, 2019 Category: Veterinary Research Source Type: research

The detection of house dust mite Dermatophagoides farinae, Der f 2 and Zen-1 allergen-specific immunoglobulin E antibodies in dogs with atopic dermatitis in Malaysia
Publication date: Available online 22 May 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): Wei Yee Chan, Gayathri Thevi Selvarajah, Mohd Mokrish Md. Ajat, Rieko Suzuki, Toshihiro TsukuiAbstractCanine atopic dermatitis (AD) is a chronic, inflammatory and pruritic allergic skin disease in dogs. House dust mites such as Dermatophagoides farinae are one of the known causative agents for the induction of canine AD worldwide. D. farinae protein Der f 2 is known as an important allergen involved in canine AD and recently, Zen-1 has also been identified as an allergenic protein. There is limited information on the p...
Source: Veterinary Immunology and Immunopathology - May 24, 2019 Category: Veterinary Research Source Type: research

Differential expression of type I interferon mRNA and protein levels induced by virulent Marek’s disease virus infection in chickens
In conclusion, our study demonstrated that MDV mostly inhibited the expression of type I IFNs in infected hosts, which may be associated to its pathogenesis. (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - May 16, 2019 Category: Veterinary Research Source Type: research

Editorial Board
Publication date: May 2019Source: Veterinary Immunology and Immunopathology, Volume 211Author(s): (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - May 11, 2019 Category: Veterinary Research Source Type: research

Mucosal expression of S100A12 (calgranulin C) and S100A8/A9 (calprotectin) and correlation with serum and fecal concentrations in dogs with chronic inflammatory enteropathy
Publication date: May 2019Source: Veterinary Immunology and Immunopathology, Volume 211Author(s): Romy M. Heilmann, Jasmin Nestler, Jutta Schwarz, Niels Grützner, Andy Ambrus, Johannes Seeger, Jan S. Suchodolski, Jörg M. Steiner, Corinne GurtnerAbstractS100A12 and S100A8/A9 (calprotectin) are released from activated mononuclear cells and belong to the group of damage associated molecular patterns. Fecal S100A12 and S100A8/A9 concentrations have been suggested as biomarkers of intestinal inflammation in dogs with chronic inflammatory enteropathies (CIE). However, the mucosal cellular infiltrate in dogs with CIE is...
Source: Veterinary Immunology and Immunopathology - May 7, 2019 Category: Veterinary Research Source Type: research

Construction and expression of a single-chain variable fragment antibody against chicken DEC 205 for targeting the bacterial expressed hemagglutinin-neuraminidase of Newcastle disease virus
The objective of the current study was the construction and bacterial expression of a recombinant antibody single-chain fragment variable (ScFv) directed against chicken DEC 205, an endocytic receptor, for use in the genetic fusion of antigens. In particular, we use as antigen the hemagglutinin-neuraminidase (HN) of Newcastle disease virus. Our results show that inoculation of chickens with HN genetically fused to the ScFv anti-DEC 205 induced an evidently higher immune response against HN, in contrast to inoculation with unconjugated HN. In addition, neutralizing antibodies against Newcastle disease virus were detected on...
Source: Veterinary Immunology and Immunopathology - May 7, 2019 Category: Veterinary Research Source Type: research