Characterization of the canine immunoglobulin heavy chain repertoire by next generation sequencing
The objective of this study was to utilize NGS for comprehensive and unbiased analysis of the IGH repertoire in healthy dogs. First, the IGH locus was searched in silico for previously unidentified genes. Second, IGH transcripts from major lymphoid organs were amplified using a 5’RACE approach without V/J primer bias. Third, amplicons were sequenced on an Illumina MiSeq platform, and data were analyzed using the ARResT/Interrogate platform. Data analysis included V/J usage, V-J pairing biases, isotype frequency, CDR3 diversity, convergent recombination, and public repertoires. The results of this study provide a comp...
Source: Veterinary Immunology and Immunopathology - July 11, 2018 Category: Veterinary Research Source Type: research

Acidic polypeptides A1a, A3, and A4 of Gly m 6 (glycinin) are allergenic for piglets
Publication date: Available online 6 June 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Shugui Zheng, Guixin Qin, Jing Chen, Fei ZhangAbstractGly m 6 (glycinin) is one of the major antigenic proteins in soybeans responsible for transient hypersensitivity to soybean meal in weaned piglets. The globulin is a hexamer consisting of subunits containing basic and acidic polypeptides. Multiple acidic polypeptides have long been demonstrated to be allergens for humans and play a key role in the overall allergenicity of Gly m 6. To date, knowledge on the allergenicity of the acidic polypeptides for piglets is very...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Glucocorticosteroids administration is associated with increased regulatory T cells in equine asthmatic lungs
Publication date: July 2018Source: Veterinary Immunology and Immunopathology, Volume 201Author(s): Roxane Boivin, Amandine Vargas, Patricia Cano, Jean-Pierre LavoieAbstractRecurrent inflammation in severe equine asthma causes a remodeling of the airways leading to incompletely reversible airway obstruction. Despite the improvement of clinical signs and lung function with glucocorticoids (GC), inflammation, translated by an increased percentage of neutrophils, persists in the airways. Regulatory T cells (Treg) have been shown to have anti-inflammatory properties and play an important role in balancing the immune response by...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Establishment and characterization of a cell line from a feline histiocytic sarcoma
Publication date: July 2018Source: Veterinary Immunology and Immunopathology, Volume 201Author(s): Ryo Miyamoto, Sena Kurita, Hiroyuki Tani, Masato Kobayashi, Soudai Sugiura, Kae Shigihara, Yuka Sato, Yoshikazu Tanaka, Kyoichi Tamura, Makoto BonkobaraAbstractFeline histiocytic sarcoma (HS) is an aggressive and uncommon tumor originating from dendritic cells/macrophages. Here, a feline HS cell line, FHS-1, was established from a case of feline HS and characterized. Immunohistochemically, FHS-1 cells were positive for vimentin and Iba-1, and negative for MHC class II and CD163. FHS-1 cells were positive for α-naphthyl ...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Fatty and hydroxycarboxylic acid receptors: The missing link of immune response and metabolism in cattle
Publication date: July 2018Source: Veterinary Immunology and Immunopathology, Volume 201Author(s): P. Alarcon, C. Manosalva, M.D. Carretta, A.I. Hidalgo, C.D. Figueroa, A. Taubert, C. Hermosilla, M.A. Hidalgo, R.A. BurgosAbstractFatty and hydroxycarboxylic acids are one of the main intermediates of energy metabolism in ruminants and critical in the milk production of cattle. High production demands on a dairy farm can induce nutritional imbalances and metabolism disorders, which have been widely associated with the onset of sterile inflammatory processes and increased susceptibility to infections. The literature suggests t...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Corrigendum to “Detection of Mycobacterium bovis infection in African buffaloes (Syncerus caffer) using QuantiFERON®-TB Gold (QFT) tubes and the Qiagen cattletype® IFN-gamma ELISA” [J. Vet. Immunol. Immunopathol. 196 (2018) 48–52]
Publication date: July 2018Source: Veterinary Immunology and Immunopathology, Volume 201Author(s): Netanya Bernitz, Charlene Clarke, Eduard O. Roos, Wynand J. Goosen, David Cooper, Paul D. van Helden, Sven D.C. Parsons, Michele A. Miller (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Editorial Board
Publication date: July 2018Source: Veterinary Immunology and Immunopathology, Volume 201Author(s): (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Effects of early feeding and dietary interventions on development of lymphoid organs and immune competence in neonatal chickens: A review
Publication date: July 2018Source: Veterinary Immunology and Immunopathology, Volume 201Author(s): Khaled Taha-Abdelaziz, Douglas C. Hodgins, Aart Lammers, Tamiru Negash Alkie, Shayan SharifAbstractWith the ongoing intensification of the poultry industry and the continuous need to control pathogens, there is a critical need to extend our understanding of the avian immune system and the role of nutritional interventions on development of immune competence in neonatal chicks. In this review, we will focus on the ontogeny of the lymphoid organs during embryonic life and the first 2 weeks post-hatch, and how early feeding prac...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Antigen-specific interferon-gamma release is decreased following the single intradermal comparative cervical skin test in African buffaloes (Syncerus caffer)
Publication date: July 2018Source: Veterinary Immunology and Immunopathology, Volume 201Author(s): C. Clarke, D. Cooper, W.J. Goosen, R. McFadyen, R.M. Warren, P.D. van Helden, S.D.C Parsons, M.A. MillerAbstractEffective disease management of wildlife relies on the strategic application of ante-mortem diagnostic tests for early identification and removal of M. bovis-infected animals. To improve diagnostic performance, interferon-gamma release assays (IGRAs) are often used in conjunction with the tuberculin skin test (TST). Since buffaloes are major maintenance hosts of M. bovis, optimal application of bovine TB diagnostic ...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Polymorphonuclear cells and reactive oxygen species in contagious bovine pleuropneumonia: New insight from in vitro investigations
Publication date: July 2018Source: Veterinary Immunology and Immunopathology, Volume 201Author(s): Giovanni Di Teodoro, Giuseppe Marruchella, Francesco Mosca, Andrea Di Provvido, Flavio Sacchini, Pietro Giorgio Tiscar, Massimo ScacchiaAbstractReactive oxygen species (ROS) are suggested to play a role in the pathogenesis of contagious bovine pleuropneumonia, a severe respiratory disorder caused by Mycoplasma mycoides subsp. mycoides (Mmm). The present study investigated the generation of ROS by different strains of Mmm, as well as their effect on the oxidative response of bovine neutrophils. The production of ROS was indire...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Epitope mapping of Salmonella flagellar hook-associated protein, FlgK, with mass spectrometry-based immuno-capture proteomics using chicken (Gallus gallus domesticus) sera
Publication date: July 2018Source: Veterinary Immunology and Immunopathology, Volume 201Author(s): Hung-Yueh Yeh, Kyoko Kojima, James A. MobleyAbstractSalmonella, a Gram-negative rod, is the leading foodborne pathogen associated with human acute bacterial gastroenteritis worldwide. The Salmonella flagellum is responsible for bacterial movement, colonization and invasion in the host gastrointestinal tract. The flagellum has a complex structure, composed of more than 35 proteins. Among them, we were interested in the flagellar hook-associated protein (FlgK), which is an immunodominant protein in chickens. In this communicati...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Evaluating the cytokine profile of the WC1+ γδ T cell subset in the ileum of cattle with the subclinical and clinical forms of MAP infection
This study demonstrates changes in the cytokine mRNA expression profile of mucosal cells in the ileum, and specifically WC1+ γδ T cells, as cattle progress to the clinical disease. The change is characterized by an increase in expression of anti-inflammatory cytokines. (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Barley produced Culicoides allergens are suitable for monitoring the immune response of horses immunized with E. coli expressed allergens
Publication date: July 2018Source: Veterinary Immunology and Immunopathology, Volume 201Author(s): Sigridur Jonsdottir, Sara Bjork Stefansdottir, Sæmundur Bjarni Kristinarson, Vilhjalmur Svansson, Jon Mar Bjornsson, Arna Runarsdottir, Bettina Wagner, Eliane Marti, Sigurbjorg TorsteinsdottirAbstractInsect bite hypersensitivity is an allergic dermatitis of horses caused by bites of Culicoides midges. Sufficient amount of pure, endotoxin-free allergens is a prerequisite for development and monitoring of preventive and therapeutic allergen immunotherapy.Aims of the study were to compare the Culicoides nubeculosus (Cul n)...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Survey of cytokines in normal canine tears by multiplex analysis: A pilot study
ConclusionThis pilot study is the first to analyze cytokines in canine tears. This study demonstrated that IL-8 is consistently detected in both diluted and undiluted samples, but undiluted samples may be superior to 1:10 diluted samples for evaluation of other cytokines in canine tears. (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

IP-10: A potential biomarker for detection of Mycobacterium bovis infection in warthogs (Phacochoerus africanus)
In this study, we obtained blood samples from warthogs in bTB endemic areas to investigate biomarkers for detection of Mycobacterium bovis infection. Warthog whole blood was incubated in QuantiFERON® TB Gold In-Tube tubes and pathogen specific release of interferon gamma (IFN-γ) and interferon gamma induced protein 10 (IP-10) was measured by a sandwich enzyme-linked immunosorbent assay. Although we were unable to measure IFN-γ, we could successfully measure IP-10. The IP-10 assay was able to distinguish between M. bovis-infected and M. bovis-culture negative warthogs, within bTB endemic areas, with an assay...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Inflammasomes in livestock and wildlife: Insights into the intersection of pathogens and natural host species
We describe current gaps in our knowledge and highlight promising areas for future research. (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Effects of a P-class CpG-ODN administered by intramuscular injection on plasma cytokines and on white blood cells of healthy horses
Publication date: July 2018Source: Veterinary Immunology and Immunopathology, Volume 201Author(s): I. Tosi, F. Bureau, F. Farnir, J.M. Denoix, P. Lekeux, T. ArtAbstractCytosine-phosphate-guanosine (CpG-ODN) has been described as a potent immunostimulatory agent in different species. No study reported the effect of a P-class CpG when administered systemically in healthy horses. The aim of this study was to evaluate the tolerance and the effect of an intramuscularly administered P-class CpG-ODN on hematology and on plasma cytokines (IFN-α, IL-10, TNF-α, IFN-γ) in 8 healthy horses. Intra-muscular CpG-ODN or ...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Effect of Marek’s disease vaccines on interferon and toll like receptors when administered in ovo
Publication date: July 2018Source: Veterinary Immunology and Immunopathology, Volume 201Author(s): Isabel M. Gimeno, Ayanna Glaize, Aneg L. CortesAbstractThe effect of two Marek’s disease (MD) vaccines on the chicken embryo immune responses were evaluated. Transcription of interferon (IFN-α, IFN-β, IFN-λ, and IFN-γ) and interferon-I receptors (IFN-AR1 and IFN-AR2), as well as transcription of toll like receptors (TLR-3, TLR-7, and TLR-21) were evaluated in the bursa, thymus, spleen and lung of 1-day-old chickens that had been vaccinated with HVT, CVI988, or sham inoculated at embryonic day 18 ...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Pathological, immunological and parasitological study of sheep vaccinated with the recombinant protein 14-3-3z and experimentally infected with Fasciola hepatica
In this study, the immunogenicity and protective capacity of a new recombinant vaccine candidate, the rFh14-3-3z protein was analysed in sheep experimentally challenged with Fasciola hepatica, in terms of fluke burden, faecal egg counts, hepatic damage and humoral immune response. Three groups of 8 animals each were used for study, group 1 was immunised with the rFh14-3-3z in Montanide adjuvant, whereas group 2 and 3 remained as adjuvant control and infection control groups, respectively. The parasitological analysis showed that no significant reduction in fluke burden, fluke size and faecal egg counts was detected. The ex...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

CIRCULATING REGULATORY T CELLS (Treg), LEPTIN AND INDUCTION OF PROINFLAMMATORY ACTIVITY IN OBESE LABRADOR RETRIEVER DOGS
Publication date: Available online 7 July 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Anna Teresa Palatucci, Diego Piantedosi, Valentina Rubino, Angela Giovazzino, Jacopo Guccione, Vlenia Pernice, Giuseppina Ruggiero, Laura Cortese, Giuseppe TerrazzanoAbstractOver-nutrition and obesity have been associated with impaired immunity and low-grade inflammation in humans and mouse models. In this context, a causal role for unbalanced T regulatory cell (Treg)-dependent mechanisms has been largely suggested.Obesity is the most common nutritional disorder in dogs. However, it is not defined whether canine obesit...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Identification of genetic variation in equine collagenous lectins using targeted resequencing
This study contributes to the growing body of evidence that pooled, high-throughput sequencing is a viable strategy for cost-effective variant discovery. (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Beneficial and detrimental impact of transplanted canine adipose-derived stem cells in a virus-induced demyelinating mouse model
Publication date: Available online 8 July 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Florian Hansmann, Nicole Jungwirth, Ning Zhang, Thomas Skripuletz, Veronika Maria Stein, Andrea Tipold, Martin Stangel, Wolfgang BaumgärtnerAbstractIn recent years stem cell therapies have been broadly applied in various disease models specifically immune mediated and degenerative diseases. Whether adipose-derived stem cells might represent a useful therapeutic option in virus-triggered central nervous system diseases has not been investigated so far. Theiler’s murine encephalomyelitis (TME) and canine diste...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Assessment of Poly(ADP-ribose) Polymerase1 (PARP1) expression and activity in cells purified from blood and milk of dairy cattle
In conclusion, these findings suggest that PARP1 is activated during mastitis, which may prove to be a useful biomarker of mastitis. (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Expression of pattern recognition receptors in porcine uterine epithelial cells in vivo and in culture
Publication date: August 2018Source: Veterinary Immunology and Immunopathology, Volume 202Author(s): Glenn Hamonic, J. Alex Pasternak, Nikki M. Forsberg, Tobias Käser, Heather L. WilsonAbstractPreservation of a pathogen free uterine environment is critical for maintaining healthy swine herds with high reproductive performance. Considering that uterine epithelial cells are the most numerous and thus likely point of cellular contact for pathogens in the uterus, we hypothesize that these cells may be critical for activating the immune system to clear uterine infections. Although uterine epithelial cells have not been wel...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

A Toxocara canis infection influences the immune response to house dust mite allergens in dogs
Conclusion & clinical relevanceThese observations suggest that a Tc infection increases the sensitization to Df in dogs. The possible protective effect against Df-induced clinical signs after allergen challenge should be confirmed in larger studies. (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Preactivation of neutrophils and systemic oxidative stress in dogs with hyperleptinemia
Publication date: August 2018Source: Veterinary Immunology and Immunopathology, Volume 202Author(s): A.M. Bosco, B.F.M. Almeida, T.C. Valadares, L. Baptistiolli, D.J. Hoffmann, A.A.F. Pereira, V.M.F. Lima, P.C. CiarliniAbstractHigh occurrence of obesity currently constitutes the main nutritional disease of the canine species. There is evidence that leptin increases during obesity in dogs. Hyperleptinemia is associated with increased neutrophil oxidative metabolism in obese humans and contributes to oxidative stress. However, in obese dogs, the probable relationship between this condition and the activation of the oxidative...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Equine mesenchymal stromal cells from different tissue sources display comparable immune-related gene expression profiles in response to interferon gamma (IFN)-γ
Publication date: August 2018Source: Veterinary Immunology and Immunopathology, Volume 202Author(s): Jennifer M. Cassano, Lisa A. Fortier, Rebecca B. Hicks, Rebecca M. Harman, Gerlinde R. Van de WalleAbstractMesenchymal stromal cells (MSC) have the therapeutic potential to decrease inflammation due to their immunomodulatory properties. They can be isolated from various tissue sources such as bone marrow, adipose tissue, and blood, but it is unknown how the tissue source of origin affects the responses of MSC to inflammatory stimuli. Here, we conceptually addressed this question by evaluating the immune-related gene express...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Relationship between the pathology of bovine intestinal tissue and current diagnostic tests for Johne’s disease
Publication date: August 2018Source: Veterinary Immunology and Immunopathology, Volume 202Author(s): Caitlin J. Jenvey, Jesse M. Hostetter, Adrienne L. Shircliff, Judith R. StabelAbstractJohne’s disease is an enteric disease caused by the intracellular pathogen Mycobacterium avium subsp. paratuberculosis (MAP). Upon translocation from the lumen of the small intestine, mycobacteria have the ability to thwart innate defense mechanisms and persist within the macrophage in the lamina propria. In an effort to understand how the pathology of disease is reflected in current diagnostic tests, immunofluorescent (IFA) labeling...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

A longitudinal study of autoantibodies against cytochrome P450 side-chain cleavage enzyme in dogs (Canis lupus familiaris) affected with hypoadrenocorticism (Addison’s disease)
This study demonstrates persistence of P450scc autoantibodies in a subset of dogs affected with hypoadrenocorticism and seroconversion over one year post-diagnosis. P450scc autoantibody reactivity in human autoimmune Addison’s disease has been associated with sex, with females having a higher prevalence, possibly due to P450scc expression in the ovary acting as an additional source of antigenic stimulation. However, there was no sex difference in autoantibody persistence in the dogs affected with hypoadrenocorticism. Autontibody persistence in dogs with hypoadrenocorticism might represent persistent pathology, due to...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Temporal evolution of anti-Clostridium antibody responses in sheep after vaccination with polyvalent clostridial vaccines
Publication date: August 2018Source: Veterinary Immunology and Immunopathology, Volume 202Author(s): Andrea Rossi, Amy Mónaco, Julio Guarnaschelli, Fernando Silveira, Andrés Iriarte, Arndt G. Benecke, José A. ChabalgoityAbstractPolyvalent clostridial vaccines, composed of a complex mixture of toxoids from up to 9 different species, are highly effective in controlling clostridial diseases in cattle and sheep. Commercially available vaccines usually state that in normal field conditions two doses administered 4 to 6 weeks apart elicit protective antibody levels that will last for one year. However, studi...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Investigation of immune cell markers in feline oral squamous cell carcinoma
Publication date: August 2018Source: Veterinary Immunology and Immunopathology, Volume 202Author(s): Ellen E. Sparger, Brian G. Murphy, Farina Mustaffa Kamal, Boaz Arzi, Diane Naydan, Chrisoula T. Skouritakis, Darren P. Cox, Katherine SkorupskiAbstractSquamous cell carcinoma is the most common oral cancer in the cat and presents as a locally aggressive lesion for which an effective therapeutic protocol remains elusive. Feline oral squamous cell carcinoma (OSCC) shares many clinical characteristics with human head and neck squamous cell carcinoma (HNSCC). Accordingly, present studies were conducted to determine similarities...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Comparison of polymeric immunoglobulin receptor between fish and mammals
Publication date: August 2018Source: Veterinary Immunology and Immunopathology, Volume 202Author(s): Xianghui Kong, Li Wang, Chao Pei, Jie Zhang, Xianliang Zhao, Li LiAbstractPolymeric immunoglobulin receptor (pIgR) functions in transporting polymeric immunoglobulin across epithelial cells into external secretion in animals. During animal evolution, fish was situated at a transition point on the phylogenetic spectrum between species possessing only innate immunity (i.e., invertebrates) and species depending heavily on adaptive immunity (i.e., mammals). Previous studies reported that fish and mammals significantly differ in...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Tumor necrosis factor-alpha protein concentrations in bronchoalveolar lavage fluid from healthy horses and horses with severe equine asthma
The objective of this study was to determine if TNF-α protein concentration differs in bronchoalveolar lavage fluid (BALF) obtained from healthy horses, horses with naturally occurring exacerbations of severe equine asthma and horses in remission from severe equine asthma. Tumor necrosis factor-alpha (TNF- α) protein concentrations were determined in BALF by commercial equine ELISA. Horses with naturally occurring exacerbation of severe equine asthma were found to have significantly lower BALF TNF-α protein concentrations than healthy horses (p = 0.0026). There was no significant difference in BALF TN...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Cell mediated immune response in goats after experimental challenge with the virulent Brucella melitensis strain 16M and the reduced virulence strain Rev. 1
The objective of the present study was to evaluate the caprine immune response over the course of infection with virulent B. melitensis strain 16 M and reduced virulence vaccine strain Rev. 1. Pregnant goats were infected at 11–14 weeks of gestation with 8 × 106 or 8 × 107 CFU of B. melitensis. Changes in granulocyte, monocyte, and mononuclear cell numbers were monitored by flow cytometry. Proliferative and functional responses of CD4+ T cells and WC1+ γδ T cells were also studied. B. melitensis 16 M infection triggered a pro-inflammatory response characterized by increased numbers...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Cell specific microvesicles vary with season and disease predisposition in healthy and previously laminitic ponies
Publication date: August 2018Source: Veterinary Immunology and Immunopathology, Volume 202Author(s): E.J.T. Finding, C. Lawson, J. Elliott, P.A. Harris, N.J. Menzies-GowAbstractMicrovesicles are small (up to 1 μm) vesicles found in plasma and other bodily fluids. They are recognised as part of the normal system of inter-cellular communication but altered numbers are also used as biomarkers of disease. Microvesicles have not been studied in detail in the horse but may be relevant to diseases such as laminitis. Identification of equine cell specific microvesicles was performed by developing a panel of cross reactive ant...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Validation of a clinically applicable flow cytometric assay for the detection of immunoglobulin associated platelets in dogs
Publication date: August 2018Source: Veterinary Immunology and Immunopathology, Volume 202Author(s): Sarah Shropshire, Steven Dow, Michael LappinAbstractThrombocytopenia is commonly encountered in veterinary practice when evaluating canine patients. It can occur in infectious, neoplastic, inflammatory, toxic, and immune-mediated conditions. Elucidating the underlying cause for thrombocytopenia can therefore represent a challenge to veterinary practitioners. Additionally, determination of whether an immune process could be contributing to a patient’s thrombocytopenia is important for refining differentials and enhanci...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Gene expression of AvBD6-10 in broiler chickens is independent of AvBD6, 9, and 10 peptide potency
This study examined whether tissue expression patterns of AvBD6-10 reflected microbial exposure and the encoded peptides a functional antimicrobial hierarchy.Gut AvBD6-10 gene expression was observed in hatch to day 21 birds, although the AvBD8-10 profiles were eclipsed by those detected in the liver and kidney tissues. In vitro challenges of chicken CHCC-OU2 cells using the gut commensal Lactobacillus johnsonii (104 CFU) did not significantly affect AvBD8-10 gene expression patterns, although upregulation (P  AvBD10 against Escherichia coli, but AvBD10 > AvBD9 > AvBD6 using Enterococcus faecalis. rAvBD10...
Source: Veterinary Immunology and Immunopathology - July 10, 2018 Category: Veterinary Research Source Type: research

Beneficial and detrimental impact of transplanted canine adipose-derived stem cells in a virus-induced demyelinating mouse model
Publication date: Available online 8 July 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Florian Hansmann, Nicole Jungwirth, Ning Zhang, Thomas Skripuletz, Veronika Maria Stein, Andrea Tipold, Martin Stangel, Wolfgang BaumgärtnerAbstractIn recent years stem cell therapies have been broadly applied in various disease models specifically immune mediated and degenerative diseases. Whether adipose-derived stem cells might represent a useful therapeutic option in virus-triggered central nervous system diseases has not been investigated so far. Theiler’s murine encephalomyelitis (TME) and canine diste...
Source: Veterinary Immunology and Immunopathology - July 9, 2018 Category: Veterinary Research Source Type: research

Pathological, immunological and parasitological study of sheep vaccinated with the recombinant protein 14-3-3z and experimentally infected with Fasciola hepatica
In this study, the immunogenicity and protective capacity of a new recombinant vaccine candidate, the rFh14-3-3z protein was analysed in sheep experimentally challenged with Fasciola hepatica, in terms of fluke burden, faecal egg counts, hepatic damage and humoral immune response. Three groups of 8 animals each were used for study, group 1 was immunised with the rFh14-3-3z in Montanide adjuvant, whereas group 2 and 3 remained as adjuvant control and infection control groups, respectively. The parasitological analysis showed that no significant reduction in fluke burden, fluke size and faecal egg counts was detected. The ex...
Source: Veterinary Immunology and Immunopathology - July 8, 2018 Category: Veterinary Research Source Type: research

CIRCULATING REGULATORY T CELLS (Treg), LEPTIN AND INDUCTION OF PROINFLAMMATORY ACTIVITY IN OBESE LABRADOR RETRIEVER DOGS
Publication date: Available online 7 July 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Anna Teresa Palatucci, Diego Piantedosi, Valentina Rubino, Angela Giovazzino, Jacopo Guccione, Vlenia Pernice, Giuseppina Ruggiero, Laura Cortese, Giuseppe TerrazzanoAbstractOver-nutrition and obesity have been associated with impaired immunity and low-grade inflammation in humans and mouse models. In this context, a causal role for unbalanced T regulatory cell (Treg)-dependent mechanisms has been largely suggested.Obesity is the most common nutritional disorder in dogs. However, it is not defined whether canine obesit...
Source: Veterinary Immunology and Immunopathology - July 8, 2018 Category: Veterinary Research Source Type: research

Validation of a clinically applicable flow cytometric assay for the detection of immunoglobulin associated platelets in dogs
Publication date: Available online 6 July 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Sarah Shropshire, Steve Dow, Michael LappinAbstractThrombocytopenia is commonly encountered in veterinary practice when evaluating canine patients. It can occur in infectious, neoplastic, inflammatory, toxic, and immune-mediated conditions. Elucidating the underlying cause for thrombocytopenia can therefore represent a challenge to veterinary practitioners. Additionally, determination of whether an immune process could be contributing to a patient’s thrombocytopenia is important for refining differentials and enh...
Source: Veterinary Immunology and Immunopathology - July 7, 2018 Category: Veterinary Research Source Type: research

Identification of genetic variation in equine collagenous lectins using targeted resequencing
This study contributes to the growing body of evidence that pooled, high-throughput sequencing is a viable strategy for cost-effective variant discovery. (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - July 7, 2018 Category: Veterinary Research Source Type: research

Acidic polypeptides A1a, A3, and A4 of Gly m 6 (glycinin) are allergenic for piglets
Publication date: Available online 6 June 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Shugui Zheng, Guixin Qin, Jing Chen, Fei ZhangAbstractGly m 6 (glycinin) is one of the major antigenic proteins in soybeans responsible for transient hypersensitivity to soybean meal in weaned piglets. The globulin is a hexamer consisting of subunits containing basic and acidic polypeptides. Multiple acidic polypeptides have long been demonstrated to be allergens for humans and play a key role in the overall allergenicity of Gly m 6. To date, knowledge on the allergenicity of the acidic polypeptides for piglets is very...
Source: Veterinary Immunology and Immunopathology - July 5, 2018 Category: Veterinary Research Source Type: research

Assessment of Poly(ADP-ribose) Polymerase1 (PARP1) expression and activity in cells purified from blood and milk of dairy cattle
In conclusion, these findings suggest that PARP1 is activated during mastitis, which may prove to be a useful biomarker of mastitis. (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - July 5, 2018 Category: Veterinary Research Source Type: research

Editorial Board
Publication date: July 2018Source: Veterinary Immunology and Immunopathology, Volume 201Author(s): (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - July 5, 2018 Category: Veterinary Research Source Type: research

Effects of early feeding and dietary interventions on development of lymphoid organs and immune competence in neonatal chickens: A review
Publication date: July 2018Source: Veterinary Immunology and Immunopathology, Volume 201Author(s): Khaled Taha-Abdelaziz, Douglas C. Hodgins, Aart Lammers, Tamiru Negash Alkie, Shayan SharifAbstractWith the ongoing intensification of the poultry industry and the continuous need to control pathogens, there is a critical need to extend our understanding of the avian immune system and the role of nutritional interventions on development of immune competence in neonatal chicks. In this review, we will focus on the ontogeny of the lymphoid organs during embryonic life and the first 2 weeks post-hatch, and how early feeding prac...
Source: Veterinary Immunology and Immunopathology - July 5, 2018 Category: Veterinary Research Source Type: research

Antigen-specific interferon-gamma release is decreased following the single intradermal comparative cervical skin test in African buffaloes (Syncerus caffer)
Publication date: July 2018Source: Veterinary Immunology and Immunopathology, Volume 201Author(s): C. Clarke, D. Cooper, W.J. Goosen, R. McFadyen, R.M. Warren, P.D. van Helden, S.D.C Parsons, M.A. MillerAbstractEffective disease management of wildlife relies on the strategic application of ante-mortem diagnostic tests for early identification and removal of M. bovis-infected animals. To improve diagnostic performance, interferon-gamma release assays (IGRAs) are often used in conjunction with the tuberculin skin test (TST). Since buffaloes are major maintenance hosts of M. bovis, optimal application of bovine TB diagnostic ...
Source: Veterinary Immunology and Immunopathology - July 5, 2018 Category: Veterinary Research Source Type: research

Polymorphonuclear cells and reactive oxygen species in contagious bovine pleuropneumonia: New insight from in vitro investigations
Publication date: July 2018Source: Veterinary Immunology and Immunopathology, Volume 201Author(s): Giovanni Di Teodoro, Giuseppe Marruchella, Francesco Mosca, Andrea Di Provvido, Flavio Sacchini, Pietro Giorgio Tiscar, Massimo ScacchiaAbstractReactive oxygen species (ROS) are suggested to play a role in the pathogenesis of contagious bovine pleuropneumonia, a severe respiratory disorder caused by Mycoplasma mycoides subsp. mycoides (Mmm). The present study investigated the generation of ROS by different strains of Mmm, as well as their effect on the oxidative response of bovine neutrophils. The production of ROS was indire...
Source: Veterinary Immunology and Immunopathology - July 5, 2018 Category: Veterinary Research Source Type: research

Epitope mapping of Salmonella flagellar hook-associated protein, FlgK, with mass spectrometry-based immuno-capture proteomics using chicken (Gallus gallus domesticus) sera
Publication date: July 2018Source: Veterinary Immunology and Immunopathology, Volume 201Author(s): Hung-Yueh Yeh, Kyoko Kojima, James A. MobleyAbstractSalmonella, a Gram-negative rod, is the leading foodborne pathogen associated with human acute bacterial gastroenteritis worldwide. The Salmonella flagellum is responsible for bacterial movement, colonization and invasion in the host gastrointestinal tract. The flagellum has a complex structure, composed of more than 35 proteins. Among them, we were interested in the flagellar hook-associated protein (FlgK), which is an immunodominant protein in chickens. In this communicati...
Source: Veterinary Immunology and Immunopathology - July 5, 2018 Category: Veterinary Research Source Type: research

Evaluating the cytokine profile of the WC1+ γδ T cell subset in the ileum of cattle with the subclinical and clinical forms of MAP infection
This study demonstrates changes in the cytokine mRNA expression profile of mucosal cells in the ileum, and specifically WC1+ γδ T cells, as cattle progress to the clinical disease. The change is characterized by an increase in expression of anti-inflammatory cytokines. (Source: Veterinary Immunology and Immunopathology)
Source: Veterinary Immunology and Immunopathology - July 5, 2018 Category: Veterinary Research Source Type: research