Method for Labeling and Reconstruction of Single Neurons Using Sindbis Virus Vectors

We describe in detail a protocol for single-neuron labeling using Sindbis virus vectors, and we provide an example of a recent project at our laboratory in which we successfully applied these methods to study thalamocortical projection neurons. Further, we discuss the strengths and limitations of Sindbis virus vectors for single neuron reconstruction, comparing them with adeno-associated virus vectors.
Source: Journal of Chemical Neuroanatomy - Category: Neuroscience Source Type: research