Effects of formaldehyde on detoxification and immune responses in silver pomfret (Pampus argenteus)

Publication date: May 2019Source: Fish & Shellfish Immunology, Volume 88Author(s): Jiabao Hu, Qijun Le, Yajun Wang, Na Yu, Xiaohuan Cao, Siwen Kuang, Man Zhang, Weiwei Gu, Yibo Sun, Yang Yang, Shanliang Xu, Xiaojun YanAbstractFormaldehyde can effectively control ectoparasites in silver pomfret (Pampus argenteus). However, there is limited information on the effects of formaldehyde treatment at a molecular level in fishes. In the present study, transcriptome profiling was conducted to investigate the effects of formaldehyde treatment (80 mg/L, bath for 1 h every day for three consecutive days) on the liver and kidney tissues of silver pomfret. A total of 617959982 clean reads were obtained and assembled into 265760 unigenes with an N50 length of 1507 bp, and the assembled unigenes were all annotated by alignment with public databases. A total of 2204 differentially expressed genes (DEGs) were detected in the liver and kidney tissues, and they included 7 detoxification- related genes and 9 immune-related genes, such as CYP450, GST, MHC I & II, and CCR. In addition, 1440 DEGs were mapped to terms in the GO database, and 1064 DEGs were mapped to the KEGG database. The expression of 4 detoxification-related genes and 6 immune-related genes in three days formaldehyde treatment were analyzed using RT-qPCR, and the antioxidant enzyme levels were also determined. The results indicate differential expression of detoxification- and immune-related genes during the three days formalde...
Source: Fish and Shellfish Immunology - Category: Biology Source Type: research