Sorting Cancer-Associated Fibroblasts (CAFs)

Featuring Our Colorectal CAFSCAFs play a central role in the Tumor Microenvironment (TME). The TME has been identified as one of the driving factors of tumor progression and invasion. Inside this microenvironment, CAFs, a type of perpetually activated fibroblasts, have been implicated to have a strong tumor modulating effect and play a key role in areas such as drug resistance.This makes CAFs a target for cancer therapies. The challenge is the TME is heterogeneous making it a challenge to derive homogeneously relevant populations for basic research and drug discovery. This new study uses ourColorectal CAFs to identify markers for isolating these populations. Martin Nurmik, Pit Ullmann, Fabien Rodriguez, Serge Haan, Elisabeth Letellier.In search of definitions: Cancer-associated fibroblasts and their markers. doi: 10.1002/ijc.32193.Images: Expression of common markers in patient-derived fibroblasts. Immunofluorescent staining of primary colon cancer fibroblasts (Neuromics, #CAF05), reveals a heterogeneous expression pattern for both αSMA/ACTA2 (abcam #ab7817, 1/200) and FAP (Santa-Cruz Biotechnology #sc-65398, 1/200), while PDGFRα (abcam #ab61219, 1/200) expression in tested cells remains relatively homogenous. Nuclei of fibroblasts were stained using DAPI (DAPI Fluoromount-G® Mounting Medium). Image is representative of thr ee independent biological experiments.Cells were imaged using a Zeiss LSM 510 Meta laser scanning confocal microscope (Carl Zeiss, Je...
Source: Neuromics - Category: Neuroscience Tags: CAFs Cancer Associated Fibroblasts CD90 colorectal CAFs Colorectal Cancer Flow cytometry PDGF Source Type: news

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In this study, we show that calorie restriction is protective against age-related increases in senescence and microglia activation and pro-inflammatory cytokine expression in an animal model of aging. Further, these protective effects mitigated age-related decline in neuroblast and neuronal production, and enhanced olfactory memory performance, a behavioral index of neurogenesis in the SVZ. Our results support the concept that calorie restriction might be an effective anti-aging intervention in the context of healthy brain aging. Greater Modest Activity in Late Life Correlates with Lower Incidence of Dementia ...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
Abstract Glucose, the key source of metabolic energy, is imported into cells by two categories of transporters: 1) facilitative glucose transporters (GLUTs) and 2) secondary active sodium-glucose cotransporters (SGLTs). Cancer cells have an increased demand for glucose uptake and utilisation compared to normal cells. Previous studies have demonstrated the overexpression of GLUTs, mainly GLUT1, in many cancer types. As the current standard positron emission tomography (PET) tracer 2-deoxy-2-(18F)fluoro-D-glucose (2-FDG) for imaging tumour cells via GLUT1 lacks in sensitivity and specificity, it may soon be replaced...
Source: Arhiv za Higijenu Rada i Toksikologiju - Category: Occupational Health Authors: Tags: Arh Hig Rada Toksikol Source Type: research
Publication date: Available online 23 July 2018Source: International Journal of PharmaceuticsAuthor(s): Mona Alibolandi, Fazileh Hoseini, Marzieh Mohammadi, Pouria Ramezani, Elham Einafshar, Seyed Mohammad Taghdisi, Mohammad Ramezani, Khalil AbnousAbstractGold NPs have great potential in biomedical applications. PAMAM dendrimers are spherical, hyper branched macromolecules which can encapsulate therapeutic molecules while stabilizing metal nanoparticle such as gold NPs. The aim of the current study was to investigate the theranostic capability of curcumin-loaded dendrimer-gold hybrid structure.Dendrimer-gold hybrid structu...
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research
Conclusion Imaging will likely play a central role in understanding immune cell trafficking to tumors, evaluating sites of potential toxicity, and determining the efficacy of cell-based therapies. We show in vivo BLI and PET/CT monitoring of CAR T cells trafficking to solid tumors. This triple reporter imaging approach allows facile monitoring of cells in preclinical studies (via fluorescence and/or BLI), and we anticipate that the [18F]FPTMP/dhfr PET probe and reporter gene pair will be useful for tracking CAR T cells in the clinical setting. Future studies will test whether trafficking of CAR T cells correlates with therapeutic response.
Source: Journal of Nuclear Medicine - Category: Nuclear Medicine Authors: Tags: Center for Therapy Excellence YIA Symposium Source Type: research
Conclusions In this study, we have developed NK cell-based theranostic platforms using radiolabeled and gold coated iron oxide nanoparticles, and demonstrated that PET/MRI/PA multimodal imaging and synergistic therapy by adoptive immunotherapy and NIR light-induced phototherapy therapy. This multimodal theranosis based on NK cells paves the way for the rational design of immuno-plasmonic theranostic platform. References 1. Zhigang Tian et al., "NK cell-based immunotherapy for malignant diseases", Cellular &Molecular Immunology, 2013, 10, 230-252. 2. Heike E. Daldrup-Link et al., "Monitoring of Natural Ki...
Source: Journal of Nuclear Medicine - Category: Nuclear Medicine Authors: Tags: Novel Multimodality and Nonradioactive Probes Source Type: research
Fight Aging! provides a weekly digest of news and commentary for thousands of subscribers interested in the latest longevity science: progress towards the medical control of aging in order to prevent age-related frailty, suffering, and disease, as well as improvements in the present understanding of what works and what doesn't work when it comes to extending healthy life. Expect to see summaries of recent advances in medical research, news from the scientific community, advocacy and fundraising initiatives to help speed work on the repair and reversal of aging, links to online resources, and much more. This content is...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
Fight Aging! provides a weekly digest of news and commentary for thousands of subscribers interested in the latest longevity science: progress towards the medical control of aging in order to prevent age-related frailty, suffering, and disease, as well as improvements in the present understanding of what works and what doesn't work when it comes to extending healthy life. Expect to see summaries of recent advances in medical research, news from the scientific community, advocacy and fundraising initiatives to help speed work on the repair and reversal of aging, links to online resources, and much more. This content is...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
WebMD wasn't a research option when Ivy Brown was diagnosed with Hodgkin's lymphoma in 1974, so her mother looked up her 12-year-old daughter's condition the old-fashioned way, in a hardcover medical volume. "It just said 'fatal,'" Brown explained. Having moved the family to London a month earlier, Brown's parents were still trying to liaise with her pediatrician in the U.S. "My father told me recently that he was sleeping with the phone on his stomach because of the time change," she said. "They were as scared as you can be when you have a child who you think you might lose," Brown explained....
Source: Science - The Huffington Post - Category: Science Source Type: news
Thoracic surgeon and robotics innovator Dr. Farid Gharagozloo believes regular early screenings and better follow-up care will significantly reduce the annual number of Americans dying of lung cancer by almost two-thirds. Gharagozloo, director of cardiothoracic surgery at Florida Hospital Celebration Health, says the medical establishment in the U.S. and patients themselves share the blame for the unreasonably high number of lung cancer deaths today. Patients need to be more assertive. Doctors need to be more aggressive. Together, they can make a big difference. "We can turn this thing around — change the who...
Source: Asbestos and Mesothelioma News - Category: Environmental Health Authors: Tags: Treatment & Doctors Source Type: news
Conclusions: This novel nanocomposite is biocompatible with potential properties to serve in magnet aided targeted drug delivery and cancer therapy.
Source: Journal of Nanobiotechnology - Category: Nanotechnology Authors: Source Type: research
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