Development of multiplex PCR and multi-color fluorescent in situ hybridization ( m -FISH) coupled protocol for detection and imaging of multi-pathogens involved in inflammatory bowel disease

ConclusionWe developed a 4-h multiplex PCR protocol, which was validated bym-FISH images, capable of detecting up to four genes from major pathogens associated with CD. The new protocol should serve as an excellent tool to support efforts to study multi-pathogens involved in CD and other autoimmune disease.
Source: Gut Pathogens - Category: Microbiology Source Type: research