A New Protocol of Type 2 Diabetes Induction and Hydrocortisone Effects on Beta-Cells Signaling in Mice

Background: There are complex methods to induce type 2 diabetes as multiple low-doses streptozotocin with high fat diet administration and other combined methods which induce modest DNA damage, leading to the incomplete destruction of β cells for insulin-resistant diabetes in mice. Objective: An innovative and simple protocol was investigated to induce type 2 diabetes by only Incre-mental Multiple Low-Doses Streptozotocin (IMLDS) in mice. The effects of hydrocortisone injection on diabetes induction and pancreatic β-cells signaling were also surveyed. Method: IMLDS designed protocol was conducted in 4 consecutive days by streptozotocin injections of 20, 40, 80 and 160 mg/kg BW/day and stress induced by hydrocortisone injections of 5, 10 and 20 mg/kg BW/day in mice, respectively. The glucose, insulin, glucagon-like peptide 1, RAC-beta serine/threonine-protein kinase and dipeptidyl peptidase IV of blood were assessed by ELISA. Results: The glucose, glucagon-like peptide 1, RAC-beta serine/threonine-protein kinase levels, and food and water intake increased (p
Source: Current Chemical Biology - Category: Biochemistry Source Type: research