Further insights into the impact of mouse follicle stage on graft outcome in an artificial ovary environment

This study involved the participation of the Gynecology Research Unit (Universit è Catholique de Louvain) and the Physiological Sciences Department (University of Brasília). Specific techniques were used to analyze the follicle recovery rate (hematoxylin–eosin staining), vascularization (CD34) and follicle ultrastructure (transmission electron microscopy [TEM] and SEM).MAIN RESULTS AND THE ROLE OF CHANCEAfter follicle isolation and encapsulation, a statistically higher percentage of normal follicles was observed in the secondary group (62%) than in the primordial –primary group (47%). Follicle recovery rates were 34% and 62% for primordial–primary and secondary follicles on D2, respectively, and 12% and 42% on D7, confirming that secondary follicles survive better than primordial–primary follicles after grafting. Concerning vascularization, both follic le stages exhibited similar vascularization to that seen in control mouse ovary on D7, but a significantly higher number of vessels and greater vessel surface area were detected in the secondary follicle group. Despite structural differences in fiber density between fibrin clots and ovarian tissue o bserved by SEM and TEM, preantral follicles appeared to be well encapsulated in the matrix, also showing a normal ultrastructure after grafting.LARGE SCALE DATANot applicable.LIMITATIONS, REASONS FOR CAUTIONAs demonstrated by our results during the isolation procedure, we encapsulated a significantly higher number of round...
Source: Molecular Human Reproduction - Category: Molecular Biology Source Type: research