Elevated phosphatase of regenerating liver 3 (PRL-3) promotes cytoskeleton reorganization, cell migration and invasion in endometrial stromal cells from endometrioma
STUDY QUESTION
Is phosphatase of regenerating liver-3 (PRL-3) associated with increased motility of endometriotic cells from endometrioma?
SUMMARY ANSWER
Elevated PRL-3 promotes cytoskeleton reorganization, cell migration and invasion of endometrial stromal cells (ESCs) from endometrioma.
WHAT IS KNOWN ALREADY
Overexpression of PRL-3 is associated with cancer cell migration, invasion and metastatic phenotype.
STUDY DESIGN, SIZE, DURATION
Primary human ESCs were isolated from eutopic endometrium of women without endometriosis (EuCo, n = 10), with histologically proven endometrioma (EuEM, n = 19) and from the cyst wall of ovarian endometriosis (OvEM, n = 26).
PARTICIPANTS/MATERIALS, SETTING, METHODS
The expression of PRL-3 in ESCs derived from EuCo, EuEM and OvEM at different phases of menstrual cycle were compared. The protein and mRNA levels of PRL-3 were examined by western blot and RT-qPCR, respectively. ESCs from OvEM were transfected with/without short hairpin RNA (shRNA) or small interfering RNA (siRNA). Additionally, a plasmid-mediated delivery system was used to achieve PRL-3 overexpression in ESCs from EuEM. The cellular distribution of F-actin and α-tubulin were examined by immunocytochemistry. Cell motility was evaluated by a transwell migration/invasion assay.
MAIN RESULTS AND THE ROLE OF CHANCE
The protein and mRNA levels of PRL-3 are significantly elevated in ESCs from OvEM compared with EuCo and EuEM. The expression of PRL-3 was not altered between pr...
Source: Human Reproduction - Category: Reproduction Medicine Authors: Zhan, H., Ma, J., Ruan, F., Bedaiwy, M. A., Peng, B., Wu, R., Lin, J. Tags: Gynaecology Source Type: research
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