Differential Clearance of Rat and Human Bone Marrow-Derived Mesenchymal Stem Cells from the Brain After Intra-arterial Infusion in Rats.

Differential Clearance of Rat and Human Bone Marrow-Derived Mesenchymal Stem Cells from the Brain After Intra-arterial Infusion in Rats. Cell Transplant. 2014 Mar 3; Authors: Khabbal J, Kerkelä E, Mitkari B, Raki M, Nystedt J, Mikkonen V, Bergström K, Laitinen S, Korhonen M, Jolkkonen J Abstract Intra-arterial (i.a.) delivery of bone marrow-derived mesenchymal stem cells (BMMSCs) has shown potential as a minimally invasive therapeutic approach for stroke. The aim of the present study was to determine the whole-body biodistribution and clearance of technetium-99m ((99m)Tc)-labeled rat and human BMMSCs after i.a. delivery in a rat model of transient middle cerebral artery occlusion (MCAO) using single photon emission computed tomography (SPECT). Our hypothesis was that xenotransplantation has a major impact upon the behavior of cells. Male RccHan:Wistar rats were subjected to sham-operation or MCAO. Twenty-four hours after surgery, BMMSCs (2x10(6) cells/animal) labeled with (99m)Tc were infused into the external carotid artery. Whole body SPECT images were acquired 20 min, 3 h and 6 h post-injection, after which rats were sacrificed and organs were collected and weighed for measurement of radioactivity. The results showed that the majority of the cells were located in the brain and especially in the ipsilateral hemisphere immediately after cell infusion both in sham-operated and MCAO rats. This was followed by fast disappearance, particularly ...
Source: Cell Transplantation - Category: Cytology Authors: Tags: Cell Transplant Source Type: research