Abstract P636: Sex Differences in Cardioprotective AT2R Expression in Diabetic Rats and Its Correlation with Myocardial Damage [Session Title: Diabetes, Nutrition and Obesity/Insulin and Metabolic Syndrome]

Diabetes mellitus (DM) is an independent risk factor for cardiovascular disease (CVD). Healthy, young women are protected from CVD, while diabetic women are more susceptible to CVD compared to age-matched diabetic men and non-diabetic women. Underlying mechanisms for this sex difference in CVD are not fully elucidated. The angiotensin II type 2 receptor (AT2R) is a member of the protective, vasodilative arm of the renin angiotensin system. The Agtr2 gene that codes for AT2R is X-linked, and increased Agtr2 expression is reported in female vasculature of rodent models. We hypothesized that a sex difference might exist in DM-associated regulation of cardiac AT2R expression. To test this, we used hyperglycemic, male and female Zucker diabetic fatty (ZDF) rats and age- and sex-matched normoglycemic Zucker lean (ZL) rats. The male ZDF (ZDF-M) rat is an established model of type 2 DM. We have reported previously that hyperglycemic, female ZDF (ZDF-F) rats had the highest body fat and lowest lean muscle mass compared to male and female lean rats (ZL-M, ZL-F) and ZDF-M. Cardiac Agtr2 expression was measured by qRT-PCR at 5-months, cardiac function by echocardiography was compared at 3- and 5-months, and histopathology of cardiac tissue was assessed at 5-months. ZL-F had a nearly 2-fold increase of Agtr2 compared to ZL-M (p<0.01). Relative to lean controls, ZDF-M had no significant change in Agtr2, while ZDF-F exhibited ~60% suppression (Rq=0.42) of Agtr2 (p<0.001). Echocardiogr...
Source: Hypertension - Category: Cardiology Authors: Tags: Session Title: Diabetes, Nutrition and Obesity/Insulin and Metabolic Syndrome Source Type: research