Molecules, Vol. 29, Pages 2053: Antioxidant and Cytotoxic Properties of Berberis vulgaris (L.) Stem Bark Dry Extract

Molecules, Vol. 29, Pages 2053: Antioxidant and Cytotoxic Properties of Berberis vulgaris (L.) Stem Bark Dry Extract Molecules doi: 10.3390/molecules29092053 Authors: Ionuț Mădălin Ivan Octavian Tudorel Olaru Violeta Popovici Carmen Lidia Chițescu Liliana Popescu Emanuela Alice Luță Elena Iuliana Ilie Lorelei Irina Brașoveanu Camelia Mia Hotnog George Mihai Nițulescu Rica Boscencu Cerasela Elena Gîrd Berberis vulgaris (L.) has remarkable ethnopharmacological properties and is widely used in traditional medicine. The present study investigated B. vulgaris stem bark (Berberidis cortex) by extraction with 50% ethanol. The main secondary metabolites were quantified, resulting in a polyphenols content of 17.6780 ± 3.9320 mg Eq tannic acid/100 g extract, phenolic acids amount of 3.3886 ± 0.3481 mg Eq chlorogenic acid/100 g extract and 78.95 µg/g berberine. The dried hydro-ethanolic extract (BVE) was thoroughly analyzed using Ultra-High-Performance Liquid Chromatography coupled with High-Resolution Mass Spectrometry (UHPLC–HRMS/MS) and HPLC, and 40 bioactive phenolic constituents were identified. Then, the antioxidant potential of BVE was evaluated using three methods. Our results could explain the protective effects of Berberidis cortex EC50FRAP = 0.1398 mg/mL, IC50ABTS = 0.0442 mg/mL, IC50DPPH = 0.2610 mg/mL compared to ascorbic acid (IC50 = 0.0165 mg/mL). Next, the acute toxicity and ...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research