Viruses, Vol. 16, Pages 674: Transcriptomic Investigation of the Virus Spectrum Carried by Midges in Border Areas of Yunnan Province

Viruses, Vol. 16, Pages 674: Transcriptomic Investigation of the Virus Spectrum Carried by Midges in Border Areas of Yunnan Province Viruses doi: 10.3390/v16050674 Authors: Lifen Yang Weichen Wu Sa Cai Jing Wang Guopeng Kuang Weihong Yang Juan Wang Xi Han Hong Pan Mang Shi Yun Feng Yunnan province in China shares its borders with three neighboring countries: Myanmar, Vietnam, and Laos. The region is characterized by a diverse climate and is known to be a suitable habitat for various arthropods, including midges which are notorious for transmitting diseases which pose significant health burdens affecting both human and animal health. A total of 431,100 midges were collected from 15 different locations in the border region of Yunnan province from 2015 to 2020. These midges were divided into 37 groups according to the collection year and sampling site. These 37 groups of midges were then homogenized to extract nucleic acid. Metatranscriptomics were used to analyze their viromes. Based on the obtained cytochrome C oxidase I gene (COI) sequences, three genera were identified, including one species of Forcipomyia, one species of Dasyhelea, and twenty-five species of Culicoides. We identified a total of 3199 viruses in five orders and 12 families, including 1305 single-stranded positive-stranded RNA viruses (+ssRNA) in two orders and seven families, 175 single-stranded negative-stranded RNA viruses (−ssRNA) in two orders and one family, and ...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research