Ultra-fast < em > in-vivo < /em > directional dark-field x-ray imaging for visualising magnetic control of particles for airway gene delivery

In this study, ultrafast directional dark-field synchrotron X-ray imaging was utlilised to understand how magnetic nanoparticles move within a live, anaesthetised, rat airway under the influence of static and moving&#xD;magnetic fields.&#xD;Main results: Magnetic nanoparticles emerging from an indwelling tracheal cannula were detectable during delivery, with dark-field imaging increasing the signal-to-noise ratio of this event by 3.5 times compared to the X-ray transmission signal. Particle movement as well as particle retention was evident. Dynamic magnetic fields could manipulate the magnetic particles in-situ.&#xD;Significance: This is the first evidence of the effectiveness of in-vivo dark-field imaging operating at these spatial and temporal resolutions, used to detect magnetic nanoparticles. These findings provide the basis for further development toward the effective use of magnetic nanoparticles, and advance their potential as an effective delivery vehicle for genetic agents in the airways of live organisms.PMID:38640914 | DOI:10.1088/1361-6560/ad40f5
Source: Physics in Medicine and Biology - Category: Physics Authors: Source Type: research