Functional analysis, diversity, and distribution of the < em > ean < /em > cluster responsible for 17 < em > β < /em > -estradiol degradation in sphingomonads

In this study, the newly isolated Sphingomonas colocasiae C3-2 could degrade E2 through both the 4,5-seco pathway and the 9,10-seco pathway. The novel gene cluster ean (including eanA, eanB1, eanC, and eanD) responsible for E2 degradation by the 4,5-seco pathway was identified; the novel two-component monooxygenase EanE1E2 can open ring B of 4-OH-E1 through the 9,10-seco pathway. The TetR family transcriptional regulator EanR acts as a transcriptional repressor of the ean cluster. The cluster ean was also found to be present in other reported E2-degrading sphingomonads, indicating the ubiquity of the E2 metabolism in the environment.PMID:38619269 | DOI:10.1128/aem.01974-23

Source: Applied and Environmental Microbiology - April 15, 2024 Category: Microbiology Authors: Mingliang Zhang Siyuan Gao Kaihua Pan Hongfei Liu Qian Li Xuekun Bai Qian Zhu Zeyou Chen Xin Yan Qing Hong Source Type: research