Antimicrobial potential of a biosurfactant gel for the prevention of mixed biofilms formed by fluconazole-resistant < em > C. albicans < /em > and methicillin-resistant < em > S. aureus < /em > in catheters

The objective of this study was to evaluate the antibiofilm activity of RLmix_Arg, both alone and incorporated in a gel prepared with Pluronic F-127, against dual-species biofilms of fluconazole-resistant C. albicans (FRCA) and methicillin-resistant S. aureus (MRSA) in impregnated catheters. Broth microdilution tests, MTT reduction assays of mature biofilms, impregnation of RLmix_Arg and its gel in peripheral venous catheters, durability tests and scanning electron microscopy (SEM) were performed. RLmix_Arg showed antimicrobial activity against Candida spp. and S. aureus, by reducing the cell viability of mixed biofilms of FRCA and MRSA, and preventing their formation in a peripheral venous catheter. The incorporation of this biosurfactant in the Pluronic F-127 gel considerably enhanced its antibiofilm activity. Thus, RLmix_Arg has potential application in gels for impregnation in peripheral venous catheters, helping to prevent development of dual-species biofilms of FRCA and MRSA.PMID:38425095 | DOI:10.1080/08927014.2024.2324028

Source: Biofouling - March 1, 2024 Category: Molecular Biology Authors: Cec ília Rocha da Silva L ívia Gurgel do Amaral Valente Sá Jo ão Batista de Andrade Neto F átima Daiana Dias Barroso Vit ória Pessoa de Farias Cabral Daniel Sampaio Rodrigues Lisandra Juv êncio da Silva Iri Sandro Pampolha Lima Lourdes P érez Ande Source Type: research