Quorum quenching activity of pentacyclic triterpenoids leads to inhibition of biofilm formation by Acinetobacter baumannii.
Abstract The quorum quenching (QQ) potential of three pentacyclic triterpenoids, glycyrrhetinic acid (GRA), ursolic acid (UA) and betulinic acid (BA), representing distinct groups of compounds, was evaluated. Violacein production by Chromobacterium violaceum and pyocyanin production by Pseudomonas aeruginosa were severely affected by GRA, UA and BA, suggesting a perturbation of N-acyl homoserine lactone (ASL) based signaling. Molecular docking analysis revealed a possible interaction between ASL-synthase and ASL-dependent transcriptional activator homologs from P. aeruginosa and Acinetobacter baumannii with common...
Source: Biofouling - October 25, 2020 Category: Molecular Biology Authors: Paul Bhattacharya S, Mitra A, Bhattacharya A, Sen A Tags: Biofouling Source Type: research

Underwater shear-based grooming of marine biofouling using a non-contact Bernoulli pad device.
Abstract The biofouling removal ability of a shear-based device was tested on two submerged surface types, Garolite G-10 and Intersleek 1100SR. Each surface was groomed at four frequencies along with a control group. The seven-week grooming study was conducted in Narragansett Bay, Rhode Island. The Bernoulli pad device uses confined radial outflow to generate both surface-normal forces to grip the submerged surface and shear stress to groom the surface without contact. An image-processing algorithm was developed and used to assess the effectiveness of the various grooming protocols, along with direct measurements ...
Source: Biofouling - October 25, 2020 Category: Molecular Biology Authors: Kamensky KM, Hellum AM, Mukherjee R, Naik A, Moisander PH Tags: Biofouling Source Type: research

Characterization of the biofilm grown on 304L stainless steel in urban wastewaters: extracellular polymeric substances (EPS) and bacterial consortia.
Abstract Characterization of the biofilm growing on stainless steel (SS) in untreated (UTUWW) and treated (TUWW) urban wastewaters was performed. In both media, the first phase of biofilm growth was aerobic, when the genera Caldimonas, Caulobacter, Terriglobus and Edaphobacter (iron oxidizing bacteria [IOB]) and the genera Bacillus, Sulfurimonas, Syntrophobacter and Desulfobacter (sulfur oxidizing bacteria [SOB]) were identified. In the second phase, established after immersion for 7 days, the high amount of EPS inhibited the access of oxygen and promoted the growth of anaerobic bacteria, which were the gen...
Source: Biofouling - October 22, 2020 Category: Molecular Biology Authors: Ziadi I, El-Bassi L, Bousselmi L, Akrout H Tags: Biofouling Source Type: research

Effect of quorum sensing and quenching molecules on inter-kingdom biofilm formation by Penicillium expansum and bacteria.
This study aimed to assess the effect of patulin, and N-(3-oxododecanoyl)-L-homoserine lactone (3-oxo-C12-HSL) on inter-kingdom biofilm formation between a filamentous fungus and bacteria isolated from drinking water. The filamentous fungus Penicillium expansum and the bacteria Acinetobacter calcoaceticus and Methylobacterium oryzae were used as model species. M. oryzae biofilm formation and development was more susceptible to the presence of the quenching molecules than A. calcoaceticus biofilms. Patulin reduced M. oryzae biofilm growth while 3-oxo-C12-HSL caused an increase after 48 h. The presence of P. expansum ...
Source: Biofouling - October 20, 2020 Category: Molecular Biology Authors: Afonso TB, Simões LC, Lima N Tags: Biofouling Source Type: research

Exogenous fungal quorum sensing molecules inhibit planktonic cell growth and modulate filamentation and biofilm formation in the Sporothrix schenckii complex.
In conclusion, farnesol, 2-phenylethanol and tyrosol have antifungal activity against planktonic and sessile cells and modulate filamentation and biofilm formation in Sporothrix spp. PMID: 33059473 [PubMed - as supplied by publisher] (Source: Biofouling)
Source: Biofouling - October 15, 2020 Category: Molecular Biology Authors: Brilhante RSN, Pereira VS, Nobre AFD, Oliveira JS, Fernandes MR, Costa ADC, Rodrigues AM, Camargo ZP, Pereira-Neto WA, Sidrim JJC, Rocha MFG Tags: Biofouling Source Type: research

Variability in in  vitro biofilm production and antimicrobial sensitivity pattern among Pasteurella multocida strains.
Variability in in vitro biofilm production and antimicrobial sensitivity pattern among Pasteurella multocida strains. Biofouling. 2020 Oct 15;:1-13 Authors: Prajapati A, Chanda MM, Dhayalan A, Yogisharadhya R, Chaudhary JK, Mohanty NN, Shivachandra SB Abstract Biofilm production, hitherto an uncharacterized feature among circulating Pasteurella multocida strains, was studied along with the antibiotic susceptibility pattern. On the basis of biofilm formation ability, all the strains were categorized into four groups under six different culture conditions: strong biofilm-forming (22%), moderate (19...
Source: Biofouling - October 15, 2020 Category: Molecular Biology Authors: Prajapati A, Chanda MM, Dhayalan A, Yogisharadhya R, Chaudhary JK, Mohanty NN, Shivachandra SB Tags: Biofouling Source Type: research

Molecules involved in motility regulation in Escherichia coli cells: a review.
Abstract The initial colonization of the host organism by commensal, probiotic, and pathogenic Escherichia coli strains is an important step in the development of infections and biofilms. Sensing and colonization of host cell surfaces are governed by flagellar and fimbriae/pili appendages, respectively. Biofilm formation confers great advantages on pathogenic E. coli cells such as protection against the host immune system, antimicrobial agents, and several environmental stress factors. The transition from planktonic to sessile physiological states involves several signaling cascades and factors responsible for the...
Source: Biofouling - October 7, 2020 Category: Molecular Biology Authors: Khan F, Tabassum N, Pham DTN, Oloketuyi SF, Kim YM Tags: Biofouling Source Type: research

Characterization of polymicrobial biofilms obtained from saliva or carious lesions in dentin.
In conclusion, both dentin and saliva can be used as an inoculum in in vitro studies of processes related to biofilm formation. PMID: 33003966 [PubMed - as supplied by publisher] (Source: Biofouling)
Source: Biofouling - September 30, 2020 Category: Molecular Biology Authors: Farias AL, Carvalho LPF, Méndez DAC, Cruvinel T, Brighenti FL Tags: Biofouling Source Type: research

Antimicrobial effects of a pulsed electromagnetic field: an in  vitro polymicrobial periodontal subgingival biofilm model.
The objective was to test the influence of a pulsed electromagnetic field (PEMF) on bacterial biofilm colonization around implants incorporated with healing abutments. Healing abutments with (test group) and without (control group) active PEMF devices were placed in a multispecies biofilm consisting of 31 different bacterial species. The biofilm composition and total bacterial counts (x105) were analyzed by checkerboard DNA-DNA hybridization. After 96 h, the mean level of 7 out of the 31 bacterial species differed significantly between groups, namely Eubacterium nodatum, Fusobacterium nucleatum ssp. nucleatum, Strep...
Source: Biofouling - September 28, 2020 Category: Molecular Biology Authors: Faveri M, Miquelleto DEC, Bueno-Silva B, Pingueiro JMS, Figueiredo LC, Dolkart O, Yakobson E, Barak S, Feres M, Shibli JA Tags: Biofouling Source Type: research

On the release of fluoride from biofilm reservoirs during a cariogenic challenge: an in situ study.
Abstract Biofilm fluoride reservoirs may be a source of fluoride to the fluid phase during a sugar challenge reducing tooth mineral loss. However, the evidence for that is conflicting and has not been studied in biofilms containing different fluoride levels. In order to test fluoride release from biofilms with distinct fluoride concentrations, biofilms were grown in situ exposed to a combination of placebo, calcium and fluoride rinses forming biofilms with no (fluoride-free rinses), low (fluoride-only rinses) or high (calcium followed by fluoride rinses) fluoride concentrations, and collected before and 5 m...
Source: Biofouling - September 28, 2020 Category: Molecular Biology Authors: Souza JGS, Del Bel Cury AA, Cury JA, Tenuta LMA Tags: Biofouling Source Type: research

Enterococcus faecalis inhibits Klebsiella pneumoniae growth in polymicrobial biofilms in a glucose-enriched medium.
This study aimed to investigate the interactions in polymicrobial biofilms of Klebsiella pneumoniae and Enterococcus faecalis, two of the most common uropathogens. Although K. pneumoniae was the most adherent strain, it could not maintain dominance in the polymicrobial biofilm due to the lactic acid produced by E. faecalis in a glucose-enriched medium. This result was supported by the use of E. faecalis V583 ldh-1/ldh-2 double mutant (non-producer of lactic acid), which did not inhibit the growth of K. pneumoniae. Lyophilized cell-free supernatants obtained from E. faecalis biofilms also showed antimicrobial/anti-biofilm a...
Source: Biofouling - September 23, 2020 Category: Molecular Biology Authors: Ballén V, Ratia C, Cepas V, Soto SM Tags: Biofouling Source Type: research

Antimicrobial activity of Curcuma xanthorrhiza nanoemulsions on Streptococcus mutans biofilms.
In this study, an optimal nanoemulsion formulation for Curcuma xanthorrhiza oil (Xan) was investigated using different sonication times. The antimicrobial effects of the nanoemulsion, the original emulsion, distilled water (DW), and Listerine, on Streptococcus mutans biofilms were compared. The optimum ultrasonic time, determined in terms of droplet size and stability, was found to be 10 min. Cell viability was the lowest on exposure to the nanoemulsion, and significantly different compared with exposure to DW or Listerine. The emulsion's effect was similar to that of the nanoemulsion, but was non-uniform with a hig...
Source: Biofouling - September 23, 2020 Category: Molecular Biology Authors: Cho MY, Kang SM, Lee ES, Kim BI Tags: Biofouling Source Type: research

Effects of Brazilian green propolis extract on planktonic cells and biofilms of multidrug-resistant strains of Klebsiella pneumoniae and Pseudomonas aeruginosa.
ra LD Abstract Propolis could represent an alternative therapeutic agent for targeting multidrug-resistant bacteria due to its antimicrobial potential. The effect of Brazilian green propolis (BGP) aqueous extract (AqExt) was evaluated on eight multidrug-resistant clinical strains of Klebsiella pneumoniae and Pseudomonas aeruginosa, as well as on one reference strain for each bacterial species. The minimum bactericidal concentration (MBC) was determined and optimal concentrations were further evaluated in comparison with 0.12% chlorhexidine. The natural extract was chemically characterized by HPLC-DAD analysis. The...
Source: Biofouling - September 20, 2020 Category: Molecular Biology Authors: Santos PBDRED, Ávila DDS, Ramos LP, Yu AR, Santos CEDR, Berretta AA, Camargo SEA, Oliveira JR, Oliveira LD Tags: Biofouling Source Type: research

Surface modification of polystyrene Petri dishes by plasma polymerized 4,7,10-trioxa-1,13-tridecanediamine for enhanced culturing and migration of bovine aortic endothelial cells.
This study investigates the efficiency of a plasma polymerized 4,7,10-trioxa-1,13-tridecanediamine (ppTTDDA) film coated on a polystyrene (PS) Petri dish, which is a biocompatible surface with carbon- and oxygen-based chemical species. The adhesion, proliferation, and migration properties of bovine aortic endothelial cells (BAECs) were profoundly enhanced in the ppTTDDA-coated PS Petri dishes without extracellular matrix (ECM) proteins, when compared with the uncoated PS Petri dishes. These observations indicate that ppTTDDA-coated PS Petri dishes can directly interact with cells, regardless of cell adhesion molecules. The...
Source: Biofouling - September 16, 2020 Category: Molecular Biology Authors: Lim H, Park Y, Seo Y, Park H, Cho YK, Jung D Tags: Biofouling Source Type: research

Effect of phosphate availability on biofilm formation in cooling towers.
In conclusion, phosphate limitation in cooling water is not a suitable strategy for CT biofouling control. PMID: 32883093 [PubMed - as supplied by publisher] (Source: Biofouling)
Source: Biofouling - September 3, 2020 Category: Molecular Biology Authors: Pinel ISM, Kim LH, Proença Borges VR, Farhat NM, Witkamp GJ, van Loosdrecht MCM, Vrouwenvelder JS Tags: Biofouling Source Type: research

Mechano-chemical coupling of irrigation enhances endodontic biofilm debridement.
Abstract The complexity of the root canal system results in areas where mechanical instrumentation is impossible during endodontic treatment. To disinfect these areas, the effect of irrigation on biofilm debridement is of great significance but has not yet been well explored. Using an in vitro Enterococcus faecalis biofilm model and a biofilm reactor, the present study provides a better understanding of the relative contributions of mechanical and chemical effects of irrigation on biofilm removal, as well as the factors influencing their coupling efficiency. The results clearly demonstrate that, the mechanical eff...
Source: Biofouling - August 31, 2020 Category: Molecular Biology Authors: Xu J, Gao Y, Meng Y, Wu W, Tsauo C, Guo T, Cao Y, Huang D, Zhou X, He J Tags: Biofouling Source Type: research

A bacterial polysaccharide biosynthesis-related gene inversely regulates larval settlement and metamorphosis of Mytilus coruscus.
Abstract Larval settlement and metamorphosis is essential for the development of marine invertebrates. Although polysaccharides are involved in larval settlement and metamorphosis of Mytilus coruscus, the molecular basis of polysaccharides underlying this progression remains largely unknown. Here, the roles of the polysaccharide biosynthesis-related gene 01912 of Pseudoalteromonas marina ECSMB14103 in the regulation of larval settlement and metamorphosis were examined by gene-knockout technique. Compared with biofilms (BFs) of the wild-type P. marina, Δ01912 BFs with a higher colanic acid (CA) content showed...
Source: Biofouling - August 25, 2020 Category: Molecular Biology Authors: Peng LH, Liang X, Chang RH, Mu JY, Chen HE, Yoshida A, Osatomi K, Yang JL Tags: Biofouling Source Type: research

Endowing antifouling properties on metal substrata by creating an artificial barrier layer based on scalable metal oxide nanostructures.
Abstract Here, by creating different types of artificial barrier layer against bacterial attachment, anti-biofouling properties were endowed on three metallic surfaces - aluminum, stainless steel and titanium. To each metallic surface, a tailored chemical oxidation process was applied to grow scalable oxide structures with an additional appropriate coating, resulting in three different types of anti-biofouling barrier, a thin water film, an air layer and an oil layer. Fluorescence images of the attached bacteria showed that the water layer improved the anti-biofouling performance up to 8-12 h and the air la...
Source: Biofouling - August 24, 2020 Category: Molecular Biology Authors: Song K, Shim J, Jung JY, Lee C, Nam Y Tags: Biofouling Source Type: research

In vitro and ex vivo biofilms of dermatophytes: a new panorama for the study of antifungal drugs.
This study describes an ex vivo model that creates an environment for dermatophyte biofilm growth, with features that resemble those of in vivo conditions, designing a new panorama for the study of antifungal susceptibility. Regarding planktonic susceptibility, MIC ranges were 0.125-1 µg ml-1 for griseofulvin and 0.000097-0.25 µg ml-1 for itraconazole and terbinafine. sMIC50 ranges were 2->512 µg ml-1 for griseofulvin and 0.25->64 µg ml-1 for itraconazole and terbinafine. CLSM images demonstrated a reduction in the amount of cells within the biofilm, but hyphae and ...
Source: Biofouling - August 24, 2020 Category: Molecular Biology Authors: Castelo-Branco DSCM, Aguiar L, Araújo GDS, Lopes RGP, Sales JA, Pereira-Neto WA, Pinheiro AQ, Paixão GC, Cordeiro RA, Sidrim JJC, Brilhante RSN, Rocha MFG Tags: Biofouling Source Type: research

Drag production mechanisms of filamentous biofilms.
Abstract Biofilms were grown on smooth acrylic surfaces for nominal incubation times of three, five, and ten weeks in a flow loop at the University of Michigan. The biofilm covered surfaces were exposed to the turbulent flow in a high-aspect ratio, fully developed channel flow facility at height-based Reynolds numbers from ReH ≈ 5,000 to 30,000. Measurements of the pressure drop along each fouled upper surface revealed that the friction drag increased from approximately 10% to 400%. The wide range in drag penalty was linked to variations in flow speed, the average thickness of the biofilms, and the level of ...
Source: Biofouling - August 17, 2020 Category: Molecular Biology Authors: Hartenberger JD, Callison EG, Gose JW, Perlin M, Ceccio SL Tags: Biofouling Source Type: research

Development of a multispecies periodontal biofilm model within a stirred bioreactor.
In conclusion, a subgingival biofilm model was developed using a stirred bioreactor, allowing the in vitro reproduction of complex microbial communities. This is an advanced model that may be useful to mimic complex clinical periodontal biofilms. PMID: 32781835 [PubMed - as supplied by publisher] (Source: Biofouling)
Source: Biofouling - August 10, 2020 Category: Molecular Biology Authors: Prado MM, Kovalski DJ, Torrez WB, Bueno-Silva B, Feres M, de Almeida J, Porto LM Tags: Biofouling Source Type: research

Synergistic antibacterial and anti-biofilm activity of nisin like bacteriocin with curcumin and cinnamaldehyde against ESBL and MBL producing clinical strains.
This study demonstrates that bacteriocin-GAM217 in combination with phytochemicals can be a potential anti-biofilm agent and thus has potential for biomedical applications. PMID: 32772715 [PubMed - as supplied by publisher] (Source: Biofouling)
Source: Biofouling - August 8, 2020 Category: Molecular Biology Authors: Sharma G, Dang S, K A, Kalia M, Gabrani R Tags: Biofouling Source Type: research

Application of zinc oxide and sodium alginate for biofouling mitigation in a membrane bioreactor treating urban wastewater.
Abstract This research aimed to mitigate fouling in membrane bioreactors (MBR) through concurrent usage of zinc oxide as an antibacterial agent (A) and sodium alginate as a hydrophilic agent (H) within a polyacrylonitrile membrane (PM) structure. The antibacterial polymeric membranes (APM) and antibacterial hydrophilic polymeric membranes (AHPM) synthesized showed a higher porosity, mechanical strength and bacterial inhibition zone, and a lower contact angle in comparison with PM membranes. EDS, SEM and AFM analyses were used to characterize the chemical, structural, and morphological properties of PM, APM, and AH...
Source: Biofouling - August 3, 2020 Category: Molecular Biology Authors: Sokhandan F, Homayoonfal M, Davar F Tags: Biofouling Source Type: research

Effect of arginine on microorganisms involved in dental caries: a systematic literature review of in  vitro studies.
Effect of arginine on microorganisms involved in dental caries: a systematic literature review of in vitro studies. Biofouling. 2020 Aug 04;:1-14 Authors: Miranda ML, Silva BNS, Salomão KB, de Oliveira AB, Gabbai-Armelin PR, Brighenti FL Abstract This systematic review aimed to discuss the effects of arginine on caries-related microorganisms in different in vitro biofilm models. The eligibility criteria were in vitro studies that evaluated the effect of arginine at different concentrations on caries-related microorganisms using biofilm models. Eighteen studies published between 2...
Source: Biofouling - August 3, 2020 Category: Molecular Biology Authors: Miranda ML, Silva BNS, Salomão KB, de Oliveira AB, Gabbai-Armelin PR, Brighenti FL Tags: Biofouling Source Type: research

Destruction of Pseudomonas aeruginosa pre-formed biofilms by cationic polymer micelles bearing silver nanoparticles.
This study evaluated the capacity for biofilm destruction of a novel combination of cationic polymer micelles formed from poly(2-(dimethylamino)ethyl methacrylate)-b-poly(ε-caprolactone)-b-poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA-PCL-PDMAEMA) triblock copolymer either alone, or loaded with silver nanoparticles (M_AgNPs). Pre-formed P. aeruginosa biofilms were incubated with either blank micelles, AgNO3, or M_AgNPs. Biofilm biomass (crystal violet assay), metabolic activity (Alamar blue reduction), structure (SEM) and viability (CLSM after Live/Dead staining, or plating for CFU) were checked. The results s...
Source: Biofouling - August 2, 2020 Category: Molecular Biology Authors: Paunova-Krasteva T, Haladjova E, Petrov P, Forys A, Trzebicka B, Topouzova-Hristova T, R Stoitsova S Tags: Biofouling Source Type: research

Effects of crosslink density in zwitterionic hydrogel coatings on their antifouling performance and susceptibility to silt uptake.
In this study, the zwitterionic monomer 3-[N-(2-methacryloyloxyethyl)-N,N-dimethylammonio] propanesulfonate (SPE) was copolymerized with increasing amounts of the photo-crosslinker benzophenon-4-yloxyethyl methacrylate (BPEMA) to systematically alter the density of crosslinks between the polymer chains. The effect of increasing crosslink density on the antifouling (AF) performance of the coatings was investigated in laboratory assays and fields tests. In both cases, the AF performance was improved by increasing the crosslinker content. The coatings reduced protein, diatom, and barnacle accumulation, and showed better resis...
Source: Biofouling - July 26, 2020 Category: Molecular Biology Authors: Koc J, Schönemann E, Wanka R, Aldred N, Clare AS, Gardner H, Swain GW, Hunsucker K, Laschewsky A, Rosenhahn A Tags: Biofouling Source Type: research

Characterization of planktonic and biofilm cells from two filamentous cyanobacteria using a shotgun proteomic approach.
gulhão FJM Abstract Cyanobacteria promote marine biofouling with significant impacts. A qualitative proteomic analysis, by LC-MS/MS, of planktonic and biofilm cells from two cyanobacteria was performed. Biofilms were formed on glass and perspex at two relevant hydrodynamic conditions for marine environments (average shear rates of 4 s-1 and 40 s-1). For both strains and surfaces, biofilm development was higher at 4 s-1. Biofilm development of Nodosilinea sp. LEGE 06145 was substantially higher than Nodosilinea sp. LEGE 06119, but no significant differences were found between surfaces. O...
Source: Biofouling - July 25, 2020 Category: Molecular Biology Authors: Romeu MJL, Domínguez-Pérez D, Almeida D, Morais J, Campos A, Vasconcelos V, Mergulhão FJM Tags: Biofouling Source Type: research

Environmental pH modulates biofilm formation and matrix composition in Candida albicans and Candida glabrata.
lva S Abstract Candida species are fungal opportunistic pathogens capable of colonizing and infecting various human anatomical sites, where they have to adapt to distinct niche-specific pH conditions. The aim of this study was to analyse the features of Candida albicans and Candida glabrata biofilms developed under neutral and vaginal acidic (pH 4) conditions. C. albicans produced thicker and more filamentous biofilms under neutral than under acidic conditions. On the other hand, the formation of biofilms by C. glabrata was potentiated by the acidic conditions suggesting the high adaptability of this species to th...
Source: Biofouling - July 15, 2020 Category: Molecular Biology Authors: Gonçalves B, Fernandes L, Henriques M, Silva S Tags: Biofouling Source Type: research

Development of a novel anti-biofilm peptide derived from profilin of Spodoptera frugiperda.
Abstract Candida yeast infections are the fourth leading cause of death worldwide. Peptides with antimicrobial activity are a promising alternative treatment for such infections. Here, the antifungal activity of a new antimicrobial peptide-PEP-IA18-was evaluated against Candida species. PEP-IA18 was designed from the primary sequence of profilin, a protein from Spodoptera frugiperda, and displayed potent activity against Candida albicans and Candida tropicalis, showing a minimum inhibitory concentration (MIC) of 2.5 µM. Furthermore, the mechanism of action of PEP-IA18 involved interaction with the cel...
Source: Biofouling - July 2, 2020 Category: Molecular Biology Authors: da Silva ACB, Sardi JCO, de Oliveira DGL, de Oliveira CFR, Dos Santos HF, Dos Santos EL, Crusca E, Cardoso MH, Franco OL, Macedo MLR Tags: Biofouling Source Type: research

Proposal for a microcosm biofilm model for the study of vulvovaginal candidiasis.
This study proposes a microcosm biofilm (MiB) model for the study of vulvovaginal candidiasis (VVC). Different conditions that mimic the vaginal environment were tested for MiB formation. The best growth conditions were obtained with samples incubated in vaginal fluid simulator medium pH 4.5 at 35 °C under a microaerophilic atmosphere. MiBs were evaluated for growth kinetics, fluconazole susceptibility and morphology. Samples containing high numbers of bacteria were analyzed for metagenomics. At 48 h, MiBs presented a higher cell density (CFU ml-1), a higher biomass and tolerance to fluconazole than their...
Source: Biofouling - July 2, 2020 Category: Molecular Biology Authors: Cordeiro RA, de Andrade ARC, Portela FVM, Pereira LMG, Moura SGB, Sampaio MD, Pereira EMA, de Melo Guedes GM, Bandeira SP, de Lima-Neto RG, Melo VMM, Brilhante RSN, Castelo-Branco DSCM, Rocha MFG, Sidrim JJC Tags: Biofouling Source Type: research

2-Hydroxy-4-methoxybenzaldehyde from Hemidesmus indicus is antagonistic to Staphylococcus epidermidis biofilm formation.
Abstract Staphylococcus epidermidis (SE) is an opportunistic nosocomial pathogen that accounts for recalcitrant device-related infections worldwide. Owing to the growing interest in plants and their secondary metabolites targeting bacterial adhesion, this study was intended to uncover the anti-biofilm potential of Hemidesmus indicus and its major constituent 2-hydroxy-4-methoxybenzaldehyde (HMB) against SE. The minimum biofilm inhibitory concentration (MBIC) of H. indicus root extract and HMB were found to be 500 and 250 µg ml-1, respectively. The results of time-dependent biofilm inhibition and matur...
Source: Biofouling - June 25, 2020 Category: Molecular Biology Authors: Kannappan A, Durgadevi R, Srinivasan R, Lagoa RJL, Packiavathy IASV, Pandian SK, Veera Ravi A Tags: Biofouling Source Type: research

A novel micellar formulation based on natural plant extracts enhances the efficacy of hydrogen peroxide against biofilms of Staphylococcus spp. and Pseudomonas aeruginosa.
Abstract The antibacterial efficacy of hydrogen peroxide encapsulated in micelles (mH2O2) against biofilms was compared with that of hydrogen peroxide alone and of three commercially available aqueous biocides. The activity of mH2O2 on 24-h biofilms of reference strains of Staphylococcus spp. and Pseudomonas aeruginosa was tested in a static microtiter plate model. The biofilms were incubated with mH2O2 (17% v/v H2O2, 2% lactic acid, 0.3% phytoextract, H2O) and its individual ingredients and compared with three aqueous biocides at different concentrations and times of exposure. After 5-min exposure, 10% mH2O2 (cor...
Source: Biofouling - June 25, 2020 Category: Molecular Biology Authors: Assadian O, Zatorska B, Presterl E, Diab-El Schahawi M Tags: Biofouling Source Type: research

Heterogeneity in the efficacy of dental chemical disinfectants on water-derived biofilms in  vitro.
Heterogeneity in the efficacy of dental chemical disinfectants on water-derived biofilms in vitro. Biofouling. 2020 Jun 25;:1-10 Authors: Zemouri C, de Soet JJ, Volgenant CMC, Crielaard W, Laheij AMGA Abstract Conditions in dental unit waterlines are favourable for biofilm growth and contamination of dental unit water. The aim of this study was to assess the effect of several chemical disinfectants on bacteria in a biofilm model. Water-derived biofilms were grown in a static biofilm model (Amsterdam Active Attachment model), using two growth media. Biofilms were challenged with Alpron/Bilpron, An...
Source: Biofouling - June 24, 2020 Category: Molecular Biology Authors: Zemouri C, de Soet JJ, Volgenant CMC, Crielaard W, Laheij AMGA Tags: Biofouling Source Type: research

Biofilms of Halobacterium salinarum as a tool for phenanthrene bioremediation.
Abstract The use of hyperhalophilic microorganisms is emerging as a sustainable alternative to clean hydrocarbon-polluted hypersaline water bodies. In line with this practice, this work reports on the ability of the archaeon Halobacterium salinarum to develop biofilms on a solid surface conditioned by the presence of phenanthrene crystals, which results in the removal of the contaminating compound. The cell surface hydrophobicity does not change during the removal process and this organism is shown to constitutively produce a surfactant molecule with specific action on aromatic hydrocarbons, both indicating that p...
Source: Biofouling - June 24, 2020 Category: Molecular Biology Authors: Di Meglio LG, Busalmen JP, Pegoraro CN, Nercessian D Tags: Biofouling Source Type: research

Human probiotic bacteria attenuate Pseudomonas aeruginosa biofilm and virulence by quorum-sensing inhibition.
This study suggests that the metabolites of L. casei and L. acidophilus could be a promising alternative to combat the pathogenicity of P. aeruginosa. PMID: 32573275 [PubMed - as supplied by publisher] (Source: Biofouling)
Source: Biofouling - June 22, 2020 Category: Molecular Biology Authors: Díaz MA, González SN, Alberto MR, Arena ME Tags: Biofouling Source Type: research

In vitro and in  vivo evaluation of the antifungal activity of fluoxetine combined with antifungals against Candida albicans biofilms and oral candidiasis.
In vitro and in vivo evaluation of the antifungal activity of fluoxetine combined with antifungals against Candida albicans biofilms and oral candidiasis. Biofouling. 2020 Jun 19;:1-12 Authors: Jiang L, Zheng L, Sun KA, Zhou P, Xu R, Gu J, Wei X Abstract Candida albicans biofilms are responsible for oral candidiasis. Fluoxetine is a widely used antidepressant, with certain anti-Candida activities. The antifungal activity of fluoxetine combined with various antifungals against C. albicans biofilms and oral candidiasis was evaluated in this study. The morphological change in the inhibition of fluox...
Source: Biofouling - June 18, 2020 Category: Molecular Biology Authors: Jiang L, Zheng L, Sun KA, Zhou P, Xu R, Gu J, Wei X Tags: Biofouling Source Type: research

Characterization and removal of biofouling from reverse osmosis membranes (ROMs) from a desalination plant in Northern Chile, using Alteromonas sp. Ni1-LEM supernatant.
This study characterizes the biofouling in the ROMs from a desalination plant and reports its effective removal using the supernatant obtained from Alteromonas sp. strain Ni1-LEM. The characterization of the bacterial community revealed that the most abundant taxa in ROMs were the genera Fulvivirga and Pseudoalteromonas, and unclassified species of the families Flavobacteriaceae and Sphingomonadaceae. This bacterial community significantly decreased upon treatment with the supernatant from Alteromonas sp. Ni1-LEM, resulting in the prevalence of the genus Pseudoalteromonas. Furthermore, this bacterial supernatant significan...
Source: Biofouling - June 15, 2020 Category: Molecular Biology Authors: Vera-Villalobos H, Pérez V, Contreras F, Alcayaga V, Avalos V, Riquelme C, Silva-Aciares F Tags: Biofouling Source Type: research

Diclofenac exhibits synergism with azoles against planktonic cells and biofilms of Candida tropicalis.
In conclusion, diclofenac presents synergism with fluconazole and voriconazole against resistant C. tropicalis strains and improves the activity of these azole drugs against biofilm formation. PMID: 32546021 [PubMed - as supplied by publisher] (Source: Biofouling)
Source: Biofouling - June 15, 2020 Category: Molecular Biology Authors: Brilhante RSN, Brasil JA, Oliveira JS, Pereira VS, Pereira-Neto WA, Sidrim JJC, Rocha MFG Tags: Biofouling Source Type: research

Aluminium oxide nanoparticles inhibit EPS production, adhesion and biofilm formation by multidrug resistant Acinetobacter baumannii.
In this study, aluminium oxide nanoparticles (Al2O3 NPs) were synthesized and characterized by TEM and EDX and shown to be spherical shaped nanoparticles with a diameter
Source: Biofouling - June 11, 2020 Category: Molecular Biology Authors: Muzammil S, Khurshid M, Nawaz I, Siddique MH, Zubair M, Nisar MA, Imran M, Hayat S Tags: Biofouling Source Type: research

Effect of essential oils on pathogenic and biofilm-forming Vibrio parahaemolyticus strains.
In this study, the effect of three essential oils (EOs) - clove oil (CO), thyme oil (TO), and garlic oil (GO), which are generally recognized as safe - on the planktonic growth, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), motility, biofilm formation, and quorum sensing (QS) of Vibrio parahaemolyticus was investigated. All three EOs showed bacteriostatic activity, with MICs in the range 0.02%-0.09% (v/v). CO and TO completely controlled planktonic growth at 0.28% and 0.08% (v/v), which is four times their MIC (4 × MIC), after 10 min, whereas GO completely cont...
Source: Biofouling - June 8, 2020 Category: Molecular Biology Authors: Mizan MFR, Ashrafudoulla M, Hossain MI, Cho HR, Ha SD Tags: Biofouling Source Type: research

An experimental test of stationary lay-up periods and simulated transit on biofouling accumulation and transfer on ships.
Abstract Biofouling accumulation on ships' submerged surfaces typically occurs during stationary periods that render surfaces more susceptible to colonization than when underway. As a result, stationary periods longer than typical port residence times (hours to days), often referred to as lay-ups, can have deleterious effects on hull maintenance strategies, which aim to minimize biofouling impacts on ship operations and the likelihood of invasive species transfers. This experimental study tested the effects of different lay-up durations on the magnitude of biofouling, before and after exposure to flow, using fouli...
Source: Biofouling - May 31, 2020 Category: Molecular Biology Authors: Davidson IC, Smith G, Ashton GV, Ruiz GM, Scianni C Tags: Biofouling Source Type: research

Proteomic profile of the saliva and plasma protein layer adsorbed on Ti-Zr alloy: the effect of sandblasted and acid-etched surface treatment.
In this study, the proteomic profile was evaluated by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Streptococcus sanguinis was used to test whether the protein layer affects bacterial adhesion. SLA treatment affected the proteomic profile, showing exclusive proteins adsorbed from saliva (14) and plasma (3). However, both groups exhibited close patterns of intensity for common proteins, molecular functions and biological processes mediated by proteins. Interestingly, Ti-ZrSLA showed higher bacterial adhesion (∼1.9 fold over) for the surface coated with plasma proteins. Therefore, SLA treatment...
Source: Biofouling - May 25, 2020 Category: Molecular Biology Authors: Souza JGS, Bertolini M, Costa RC, Lima CV, Barão VAR Tags: Biofouling Source Type: research

Antimicrobial activity and antibiotic synergy of a biphosphinic ruthenium complex against clinically relevant bacteria.
Abstract The aim of this study was to investigate the antibacterial activity, antibiotic-associated synergy, and anti-biofilm activity of the ruthenium complex, cis-[RuCl2 (dppb) (bqdi)]2+ (RuNN). RuNN exhibited antimicrobial activity against Gram-positive bacteria with minimum inhibitory concentration (MIC) values ranging from 15.6 to 62.5 µg ml-1 and minimum bactericidal concentration (MBC) values ranging from 62.5 to 125 µg ml-1. A synergistic effect against Staphylococcus spp. was observed when RuNN was combined with ampicillin, and the range of associated fractional inhibitory concen...
Source: Biofouling - May 24, 2020 Category: Molecular Biology Authors: Andrade AL, de Vasconcelos MA, Arruda FVS, do Nascimento Neto LG, Carvalho JMDS, Gondim ACS, Lopes LGF, Sousa EHS, Teixeira EH Tags: Biofouling Source Type: research

Development of a high throughput and low cost model for the study of semi-dry biofilms.
This study highlights the importance of conditions maintained in bacterial growth as they affect biofilm phenotype and behaviour. PMID: 32441116 [PubMed - as supplied by publisher] (Source: Biofouling)
Source: Biofouling - May 21, 2020 Category: Molecular Biology Authors: Amaeze N, Akinbobola A, Chukwuemeka V, Abalkhaila A, Ramage G, Kean R, Staines H, Williams C, Mackay W Tags: Biofouling Source Type: research

Physico-chemical and antimicrobial properties and the shelf life of experimental endodontic sealers containing metal methacrylates.
The objective of this study was to evaluate the physico-chemical and antimicrobial properties of a dual polymerization experimental endodontic sealer (E) and experimental sealers containing dibutyltin methacrylate (Sn2+) (ETs) or calcium methacrylate (Ca2+) (ECs). The pH and ion release levels of the sealers were measured. The dimensional stability was evaluated in accordance with ISO 6876. Biofilm growth inhibition was evaluated using confocal laser scanning microscopy (CLSM). Biofilm viability analysis was performed using the SYTO 9 technique. The shelf life was evaluated through the degree of conversion and film thickne...
Source: Biofouling - May 21, 2020 Category: Molecular Biology Authors: Guimarães VBDS, Barboza ADS, Cuevas-Suárez CE, Collares T, Larré Oliveira T, Ribeiro AS, Lisboa MT, Pappen FG, Lund RG Tags: Biofouling Source Type: research

Continuous flow system for biofilm formation using controlled concentrations of Pseudomonas putida from chicken carcass and coupled to electrochemical impedance detection.
Abstract Most studies dealing with monitoring the dynamics of biofilm formation use microbial suspensions at high concentrations. These conditions do not always represent food or water distribution systems. A continuous flow system capable of controlling the concentration of the microbial suspension stream from 104 to 106 CFU ml-1 is reported. Pseudomonas putida biofilms formed using 100-fold, 1,000-fold or 10,000-fold diluted bacterial suspensions were monitored in-line by electrochemical impedance spectroscopy (EIS) and total plate counts. Randles equivalent circuit model and a modified Randles model with biofil...
Source: Biofouling - May 20, 2020 Category: Molecular Biology Authors: Yang D, Reyes-De-Corcuera JI Tags: Biofouling Source Type: research

Preparation of a mesoporous silica quorum quenching medium for wastewater treatment using a membrane bioreactor.
Abstract Various quorum quenching (QQ) media have been developed to mitigate membrane biofouling in a membrane bioreactor (MBR). However, most are expensive, unstable and easily trapped in hollow fibre membranes. Here, a sol-gel method was used to develop a mesoporous silica medium entrapping a QQ bacterial strain (Rhodococcus sp. BH4). The new silica QQ medium was able to remove quorum sensing signalling molecules via both adsorption (owing to their mesoporous hydrophobic structure) and decomposition with an enzyme (lactonase), preventing MBR biofouling without affecting the water quality. It also demonstrated a ...
Source: Biofouling - May 18, 2020 Category: Molecular Biology Authors: Lee K, Choo KH, Ng HY, Lee CH Tags: Biofouling Source Type: research

Amphiphilic hydrolyzable polydimethylsiloxane-b-poly(ethyleneglycol methacrylate-co-trialkylsilyl methacrylate) block copolymers for marine coatings. II. Antifouling laboratory tests and field trials.
Abstract Poly(dimethylsiloxane) (PDMS) elastomer coatings containing an amphiphilic hydrolyzable diblock copolymer additive were prepared and their potential as marine antifouling and antiadhesion materials was tested. The block copolymer additive consisted of a PDMS first block and a random poly(trialkylsilyl methacrylate (TRSiMA, R = butyl, isopropyl)-co-poly(ethyleneglycol) methacrylate (PEGMA) copolymer second block. PDMS-b-TRSiMA block copolymer additives without PEGMA units were also used as additives. The amphiphilic character of the coating surface was assessed in water using the captive air ...
Source: Biofouling - May 18, 2020 Category: Molecular Biology Authors: Guazzelli E, Martinelli E, Pelloquet L, Briand JF, Margaillan A, Bunet R, Galli G, Bressy C Tags: Biofouling Source Type: research

Antifungal evaluation of traditional herbal monomers and their potential for inducing cell wall remodeling in Candida albicans and Candida auris.
Abstract Traditional herbal monomers (THMs) are widely distributed in many traditional Chinese formulas (TCFs) and decoctions (TCDs) and are frequently used for the prevention and treatment of fungal infections. The antifungal activities of five common THMs, including sodium houttuyfonate (SH), berberine (BER), palmatine (PAL), jatrorrhizine (JAT) and cinnamaldehyde (CIN), and their potential for inducing cell wall remodeling (CWR), were evaluated against Candida albicans SC5314 and Candida auris 12372. SH/CIN plus BER/PAL/JAT showed synergistic antifungal activity against both Candida isolates. Furthermore, SH-as...
Source: Biofouling - May 14, 2020 Category: Molecular Biology Authors: Liu J, Li Q, Wang C, Shao J, Wang T, Wu D, Ma K, Yan G, Yin D Tags: Biofouling Source Type: research

Ship hull in-water cleaning and its effects on fouling-control coatings.
Abstract Today, ship hull fouling is managed through fouling-control coatings, complemented with in-water cleaning. During cleaning, coating damage and wear must be avoided, for maximum coating lifetime and reduced antifoulant release. When possible, cleaning should target early stages of fouling, using minimal forces. However, such forces, and their effects on coatings, have not yet been fully quantified. In this one-year study, minimal cleaning forces were determined using a newly-designed immersed waterjet. The results show that bi-monthly/monthly cleaning, with maximum wall shear stress up to ∼1.3 k...
Source: Biofouling - May 12, 2020 Category: Molecular Biology Authors: Oliveira DR, Granhag L Tags: Biofouling Source Type: research