2. Personalized sequencing assays for cerebrospinal fluid liquid biopsies in children with brain tumors

Conventional methods for monitoring children with high-grade central nervous system (CNS) tumors include magnetic resonance imaging and cytological assessment of cerebrospinal fluid (CSF), but these methods often miss detection of minimal residual disease (MRD). Molecular techniques have evolved, allowing use of next-generation sequencing (NGS) to detect tumor-derived cell-free DNA (cfDNA) in peripheral biofluids (e.g., blood, urine, CSF) - coined `liquid biopsies.' Here, we describe the development of personalized sequencing assays for CSF liquid biopsies using the patient's tumor DNA profile known from prior exome sequencing.

Source: Cancer Genetics and Cytogenetics - November 1, 2023 Category: Genetics & Stem Cells Authors: Katherine Miller, Sarah Wilson, Adithe Rivaldi, Olivia Grischow, Jocelyn Lucyshyn, Lakshmi Venkata, Kyle Voytovich, Heather Costello, Peter Chang, Ben Kelly, James Fitch, Margot Lazow, Anthony Miller, Elaine Mardis Source Type: research