Isolation and Characterization of Single-Domain Antibodies from Immune Phage Display Libraries

We present and discuss comprehensive, step-by-step methods for isolating and characterization of antigen-specific VHHs. This includes protocols for expression, biotinylation, purification, and characterization of the isolated VHHs. To demonstrate the feasibility of the entire strategy, we present examples of VHHs previously isolated and characterized in our laboratory.PMID:37679618 | DOI:10.1007/978-1-0716-3381-6_7

Source: Mol Biol Cell - September 7, 2023 Category: Molecular Biology Authors: Martin A Rossotti Frederic Trempe Henk van Faassen Greg Hussack Mehdi Arbabi-Ghahroudi Source Type: research