Viruses, Vol. 15, Pages 1798: Deep Sequencing to Reveal Phylo-Geographic Relationships of Juquitiba Virus in Paraguay

Viruses, Vol. 15, Pages 1798: Deep Sequencing to Reveal Phylo-Geographic Relationships of Juquitiba Virus in Paraguay Viruses doi: 10.3390/v15091798 Authors: Evans Ifebuche Nnamani Briana Spruill-Harrell Evan Peter Williams Mariah K. Taylor Robert D. Owen Colleen B. Jonsson Several hantaviruses result in zoonotic infections of significant public health concern, causing hemorrhagic fever with renal syndrome (HFRS) or hantavirus cardiopulmonary syndrome (HCPS) in the Old and New World, respectively. Given a 35% case fatality rate, disease-causing New World hantaviruses require a greater understanding of their biology, genetic diversity, and geographical distribution. Juquitiba hantaviruses have been identified in Oligoryzomys nigripes in Brazil, Paraguay, and Uruguay. Brazil has reported the most HCPS cases associated with this virus. We used a multiplexed, amplicon-based PCR strategy to screen and deep-sequence the virus harbored within lung tissues collected from Oligoryzomys species during rodent field collections in southern (Itapúa) and western (Boquerón) Paraguay. No Juquitiba-like hantaviruses were identified in Boquerón. Herein, we report the full-length S and M segments of the Juquitiba hantaviruses identified in Paraguay from O. nigripes. We also report the phylogenetic relationships of the Juquitiba hantaviruses in rodents collected from Itapúa with those previously collected in Canindey&am...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research