Anti-inflammatory mechanisms of eucalyptol rich Eucalyptus globulus essential oil alone and in combination with flurbiprofen

This study was designed to evaluate the anti-inflammatory, analgesic and anti-pyretic properties ofEucalyptus globulus essential oil alone and in combination with flurbiprofen. GC –MS analysis was performed to screen chemical composition of oil. In vitro anti-inflammatory assay (membrane stabilization assay) and in vivo inflammatory acute  (carrageenan and histamine-induced paw oedema) and chronic (cotton pellet-induced granuloma and Complete Freund’s adjuvant-induced arthritis) models were performed to check anti-inflammatory properties. Acetic acid-induced algesia and yeast-induced pyrexia models were performed to check a nalgesic and anti-pyretic properties. qRT-PCR was performed to study the effect of treatments on the expression of inflammatory biomarkers. GC–MS analysis ofE. globulus essential oil showed the presence of eucalyptol along with other active biomolecules. 500  + 10 mg/kg of oil-drug combination showed significantly (p <  0.05) better in vitro membrane stabilization effects as compared with groups treated with 500 mg/kg ofE. globulus oil and  10 mg/kg of Flurbiprofen alone. 500 + 10 mg/kg of oil-drug combination showed significantly (p <  0.05) better anti-inflammatory, analgesic and antipyretic effects as compared to 500 mg/kg ofE. globulus oil alone in all in vivo models. When comparison was  done between 500 + 10 mg/kg of oil-drug combination-treated and 10 mg/kg Flurbiprofen-treated group, the former g...
Source: Inflammopharmacology - Category: Drugs & Pharmacology Source Type: research