Lessons from resistance analysis in clinical trials of IV zanamivir

In this study ten resistance substitutions, two of which were treatment emergent, were detected by NGS that otherwise would not have been detectable by Sanger sequencing. None of the substitutions were present at any other timepoints analysed. The effect these mutations have on clinical response is difficult to characterize; in fact, all patients from which these variants were isolated had a successful clinical outcome and the effect on clinical response was therefore likely minimal. Although NGS is becoming a routine method for nucleic acid sequencing and will detect substitutions previously undetected by Sanger sequencing, the value of this technique in identifying minority species with resistance substitutions that are clinically meaningful remains to be demonstrated, particularly with acute infections such as influenza.PMID:36610656 | DOI:10.1016/j.virusres.2023.199039

Source: Virus Research - January 7, 2023 Category: Virology Authors: Phillip J Yates Nalini Mehta Helen A Watson Amanda F Peppercorn Source Type: research