World Journal of Microbiology and Biotechnology 
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This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.Stalk rot species diversity and molecular phylogeny associated with diseased maize in India
AbstractStalk rot disease is a major constraint in maize production and till date reported to be caused by two to three species of phytopathogenic fungi but, in our present study, we disclose the first report of stalk rot is caused by complex species of phytopathogens, which belongs to five different genera. Therefore, to substantiate these findings, a total of 105 diseased samples of maize were collected from 21 different locations in six different geographical locations of India from which 48 isolates were used for the research study. Morphological features such as pigmentation, colony color, type of mycelium and pattern...
Source: World Journal of Microbiology and Biotechnology - April 29, 2024 Category: Microbiology Source Type: research
Rutin-coated zinc oxide nanoparticles: a promising antivirulence formulation against pathogenic bacteria
This study demonstrates the antivirulence features of ZnO-Rutin NPs against pathogenic bacteria which can be associated with their QS inhibitory effects. (Source: World Journal of Microbiology and Biotechnology)
Source: World Journal of Microbiology and Biotechnology - April 29, 2024 Category: Microbiology Source Type: research
Biofilm-disrupting effects of phage endolysins LysAm24, LysAp22, LysECD7, and LysSi3: breakdown the matrix
AbstractThe ability of most opportunistic bacteria to form biofilms, coupled with antimicrobial resistance, hinder the efforts to control widespread infections, resulting in high risks of negative outcomes and economic costs. Endolysins are promising compounds that efficiently combat bacteria, including multidrug-resistant strains and biofilms, without a low probability of subsequent emergence of stable endolysin-resistant phenotypes. However, the details of antibiofilm effects of these enzymes are poorly understood. To elucidate the interactions of bacteriophage endolysins LysAm24, LysAp22, LysECD7, and LysSi3 with bacter...
Source: World Journal of Microbiology and Biotechnology - April 29, 2024 Category: Microbiology Source Type: research
Effect of Ba2+ on the biomineralization of Ca2+ and Mg2+ ions induced by Bacillus licheniformis
In this study,Bacillus licheniformis was used to bio-precipitate calcium, magnesium, and barium ions. The effects of barium ions on the physiological and biochemical characteristics of bacteria, as well as the components of extracellular polymers and mineral characteristics, were also studied in systems containing coexisting barium, calcium, and magnesium ions. The results show that the increasing concentrations of barium ions decreased pH, carbonic anhydrase activity, and concentrations of bicarbonate and carbonate ions, while it increased the contents of humic acids, proteins, polysaccharides, and DNA in extracellular po...
Source: World Journal of Microbiology and Biotechnology - April 26, 2024 Category: Microbiology Source Type: research
DNA adduct formation in Saccharomyces cerevisiae following exposure to environmental pollutants, as in vivo model for molecular toxicity studies
AbstractDNA adduction in the model yeastSaccharomyces cerevisiae was investigated after exposure to the fungicide penconazole and the reference genotoxic compound benzo(a)pyrene, for validating yeasts as a tool for molecular toxicity studies, particularly of environmental pollution. The effect of the toxicants on the yeast ’s growth kinetics was determined as an indicator of cytotoxicity. Fermentative cultures ofS. cerevisiae were exposed to 2 ppm of Penconazole during different phases of growth; while 0.2 and 2 ppm of benzo(a)pyrene were applied to the culture medium before inoculation and on exponential cultures. Ex...
Source: World Journal of Microbiology and Biotechnology - April 26, 2024 Category: Microbiology Source Type: research
High-level production of chitinase by multi-strategy combination optimization in Bacillus licheniformis
The objective of this study was to utilize the extracellular chitinase fromBacillus thuringiensis as the target, andBacillus licheniformis as the expression host to achieve heterologous expression of ChiA with enhanced activity. Initially, through structural analysis and molecular dynamics simulation, we identified key amino acids to improve the enzymatic performance of chitinase, and the specific activity of chitinase mutant D116N/E118N was 48% higher than that of the natural enzyme, with concomitant enhancements in thermostability and pH stability. Subsequently, the expression elements of ChiA(D116N/E118N) were screened ...
Source: World Journal of Microbiology and Biotechnology - April 26, 2024 Category: Microbiology Source Type: research
Histone H2B lysine 122 and lysine 130, as the putative targets of Penicillium oxalicum LaeA, play important roles in asexual development, expression of secondary metabolite gene clusters, and extracellular glycoside hydrolase synthesis
AbstractCore histones in the nucleosome are subject to a wide variety of posttranslational modifications (PTMs), such as methylation, phosphorylation, ubiquitylation, and acetylation, all of which are crucial in shaping the structure of the chromatin and the expression of the target genes. A putative histone methyltransferase LaeA/Lae1, which is conserved in numerous filamentous fungi, functions as a global regulator of fungal growth, virulence, secondary metabolite formation, and the production of extracellular glycoside hydrolases (GHs). LaeA ’s direct histone targets, however, were not yet recognized. Previous researc...
Source: World Journal of Microbiology and Biotechnology - April 26, 2024 Category: Microbiology Source Type: research
Role of oxalic acid in fungal and bacterial metabolism and its biotechnological potential
AbstractOxalic acid and oxalates are secondary metabolites secreted to the surrounding environment by fungi, bacteria, and plants. Oxalates are linked to a variety of processes in soil, e.g. nutrient availability, weathering of minerals, or precipitation of metal oxalates. Oxalates are also mentioned among low-molecular weight compounds involved indirectly in the degradation of the lignocellulose complex by fungi, which are considered to be the most effective degraders of wood. The active regulation of the oxalic acid concentration is linked with enzymatic activities; hence, the biochemistry of microbial biosynthesis and d...
Source: World Journal of Microbiology and Biotechnology - April 25, 2024 Category: Microbiology Source Type: research
Genetic diversity of KPC-2-producing Klebsiella pneumoniae complex from aquatic ecosystems
In this study,blaKPC-2-positiveKlebsiella pneumoniae complex strains were isolated from different anthropogenically affected aquatic ecosystems and characterized using phenotypic, molecular, and genomic methods.K. pneumoniae complex strains exhibited multidrug-resistant and extensively drug-resistant profiles, spotlighting the resistance to carbapenems, ceftazidime-avibactam, colistin, and tigecycline, which are recognized as last-line antimicrobial treatment options. Molecular analysis showed the presence of several antimicrobial resistance, virulence, and metal tolerance genes. In-depth analysis showed that theblaKPC-2 g...
Source: World Journal of Microbiology and Biotechnology - April 24, 2024 Category: Microbiology Source Type: research
Efficient gene editing in the slow-growing, non-sporulating, melanized, endophytic fungus Berkleasmium sp. Dzf12 using a CRISPR/Cas9 system
AbstractThe endophytic fungusBerkleasmium sp. Dzf12 that was isolated fromDioscorea zingiberensis, is a proficient producer of palmarumycins, which are intriguing polyketides of the spirobisnaphthalene class. These compounds displayed a wide range of bioactivities, including antibacterial, antifungal, and cytotoxic activities. However, conventional genetic manipulation ofBerkleasmium sp. Dzf12 is difficult and inefficient, partially due to the slow-growing, non-sporulating, and highly pigmented behavior of this fungus. Herein, we developed a CRISPR/Cas9 system suitable for gene editing inBerkleasmium sp. Dzf12. The protopl...
Source: World Journal of Microbiology and Biotechnology - April 23, 2024 Category: Microbiology Source Type: research
Evaluation of multi-heavy metal tolerance traits of soil-borne fungi for simultaneous removal of hazardous metals
The objective of the present study was to isolate the fungi from the agricultural soil of Kashmir valley, investigate their multi-metal tolerance to heavy metals and evaluate the metal uptake capacities of the resistant fungi. The fungi were isolated and identified on the basis of morphological and molecular approach (ITS1 and ITS4). The tolerance limits of the isolated fungal strains to various doses of lead (Pb), cadmium (Cd), zinc (Zn), chromium (Cr), copper (Cu), nickel (Ni), and cobalt (Co) was evaluated. Five fungal strains,Aspergillus niger, Fusarium oxysporum, Fusarium verticillioides,Aspergillus fischeri, Epicoccu...
Source: World Journal of Microbiology and Biotechnology - April 22, 2024 Category: Microbiology Source Type: research
Bacterial extracellular vesicles: biotechnological perspective for enhanced productivity
AbstractBacterial extracellular vesicles (BEVs) are non-replicative nanostructures released by Gram-negative and Gram-positive bacteria as a survival mechanism and inter- and intraspecific communication mechanism. Due to BEVs physical, biochemical, and biofunctional characteristics, there is interest in producing and using them in developing new therapeutics, vaccines, or delivery systems. However, BEV release is typically low, limiting their application. Here, we provide a biotechnological perspective to enhance BEV production, highlighting current strategies. The strategies include the production of hypervesiculating str...
Source: World Journal of Microbiology and Biotechnology - April 20, 2024 Category: Microbiology Source Type: research
Growth-dependent cr(VI) reduction by Alteromonas sp. ORB2 under haloalkaline conditions: toxicity, removal mechanism and effect of heavy metals
This study investigated Cr(VI) reduction under complex haloalkaline conditions by anAlteromonas sp. ORB2 isolated from aerobic granular sludge cultivated from the seawater-microbiome. Optimum growth ofAlteromonas sp. ORB2 was observed under haloalkaline conditions at 3.5 –9.5% NaCl and pH 7–11. The bacterial growth in normal culture conditions (3.5% NaCl; pH 7.6) was not inhibited by 100 mg/l Cr(VI)/ As(V)/ Pb(II), 50 mg/l Cu(II) or 5 mg/l Cd(II). Near complete reduction of 100 mg/l Cr(VI) was achieved within 24 h at 3.5–7.5% NaCl and pH 8–11. Cr(VI) re duction byAlteromonassp. ORB2 was not inhibited by 100 ...
Source: World Journal of Microbiology and Biotechnology - April 17, 2024 Category: Microbiology Source Type: research
Metagenomic investigations into the microbial consortia, degradation pathways, and enzyme systems involved in the biodegradation of plastics in a tropical lentic pond sediment
AbstractThe exploitation of exciting features of plastics for diverse applications has resulted in significant plastic waste generation, which negatively impacts environmental compartments, metabolic processes, and the well-being of aquatic ecosystems biota. A shotgun metagenomic approach was deployed to investigate the microbial consortia, degradation pathways, and enzyme systems involved in the degradation of plastics in a tropical lentic pond sediment (APS). Functional annotation of the APS proteome (ORFs) using the PlasticDB database revealed annotation of 1015 proteins of enzymes such as depolymerase, esterase, lipase...
Source: World Journal of Microbiology and Biotechnology - April 17, 2024 Category: Microbiology Source Type: research
Co-production of metallo- β-lactamase and OXA-type β-lactamases in carbapenem-resistant Acinetobacter baumannii clinical isolates in North East India
This study aimed to investigate the antibiotic resistance pattern of carbapenem-resistantA. baumannii isolates collected from different clinical settings in North East India, focusing on their genotypic and phenotypic resistance profiles. A total of 172 multidrug-resistantA. baumannii isolates were collected and subjected to antibiotic susceptibility test using the Kirby –Bauer disk diffusion method. Various phenotypic tests were performed to detect extended-spectrum β-lactamase (ESBL), metallo-β-lactamase (MBL), class C AmpC β-lactamase (AmpC), and carbapenem hydrolyzing class D β-lactamase (CHDL) production among t...
Source: World Journal of Microbiology and Biotechnology - April 17, 2024 Category: Microbiology Source Type: research
