Magnetically modified bacteriophage-triggered ATP release activated EXPAR-CRISPR/Cas14a system for visual detection of Burkholderia pseudomallei
Biosens Bioelectron. 2024 Apr 25;257:116334. doi: 10.1016/j.bios.2024.116334. Online ahead of print.ABSTRACTBurkholderia pseudomallei, widely distributed in tropical and subtropical ecosystems, is capable of causing the fatal zoonotic disease melioidosis and exhibiting a global trend of dissemination. Rapid and sensitive detection of B. pseudomallei is essential for environmental monitoring as well as infection control. Here, we developed an innovative biosensor for quantitatively detecting B. pseudomallei relies on ATP released triggered by bacteriophage-induced bacteria lysis. The lytic bacteriophage vB_BpP_HN01, with hi...
Source: Biosensors and Bioelectronics - April 28, 2024 Category: Biotechnology Authors: Juan Yao Zhang Zhang Hua Pei Ting Zhang Yuping Ruan Chenyuan Liu Yongcan Guo Shuo Gu Qianfeng Xia Source Type: research
Comparison of extracellular vesicle isolation methods for the study of exosome cargo within Toxocara canis and Toxocara cati excretory secretory (TES) products
Exp Parasitol. 2024 Apr 27;261:108765. doi: 10.1016/j.exppara.2024.108765. Online ahead of print.ABSTRACTToxocara is a genus of nematodes, which infects a variety of hosts, principally dogs and cats, with potential zoonotic risks to humans. Toxocara spp. larvae are capable of migrating throughout the host tissues, eliciting eosinophilic and granulomatous reactions, while surviving for extended periods of time, unchanged, in the host. It is postulated that larvae are capable of altering the host's immune response through the release of excretory-secretory products, containing both proteins and extracellular vesicles (EVs). ...
Source: Experimental Parasitology - April 28, 2024 Category: Parasitology Authors: Timothy K Wu Janice L Liotta Dwight D Bowman Source Type: research
Comparison of extracellular vesicle isolation methods for the study of exosome cargo within Toxocara canis and Toxocara cati excretory secretory (TES) products
Exp Parasitol. 2024 Apr 27;261:108765. doi: 10.1016/j.exppara.2024.108765. Online ahead of print.ABSTRACTToxocara is a genus of nematodes, which infects a variety of hosts, principally dogs and cats, with potential zoonotic risks to humans. Toxocara spp. larvae are capable of migrating throughout the host tissues, eliciting eosinophilic and granulomatous reactions, while surviving for extended periods of time, unchanged, in the host. It is postulated that larvae are capable of altering the host's immune response through the release of excretory-secretory products, containing both proteins and extracellular vesicles (EVs). ...
Source: Experimental Parasitology - April 28, 2024 Category: Parasitology Authors: Timothy K Wu Janice L Liotta Dwight D Bowman Source Type: research
Comparison of extracellular vesicle isolation methods for the study of exosome cargo within Toxocara canis and Toxocara cati excretory secretory (TES) products
Exp Parasitol. 2024 Apr 27;261:108765. doi: 10.1016/j.exppara.2024.108765. Online ahead of print.ABSTRACTToxocara is a genus of nematodes, which infects a variety of hosts, principally dogs and cats, with potential zoonotic risks to humans. Toxocara spp. larvae are capable of migrating throughout the host tissues, eliciting eosinophilic and granulomatous reactions, while surviving for extended periods of time, unchanged, in the host. It is postulated that larvae are capable of altering the host's immune response through the release of excretory-secretory products, containing both proteins and extracellular vesicles (EVs). ...
Source: Experimental Parasitology - April 28, 2024 Category: Parasitology Authors: Timothy K Wu Janice L Liotta Dwight D Bowman Source Type: research
Viruses, Vol. 16, Pages 697: SARS-CoV-2 Omicron: Viral Evolution, Immune Evasion, and Alternative Durable Therapeutic Strategies
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Tong-Ming Fu
Since the SARS-CoV-2 Omicron virus has gained dominance worldwide, its continual evolution with unpredictable mutations and patterns has revoked all authorized immunotherapeutics. Rapid viral evolution has also necessitated several rounds of vaccine updates in order to provide adequate immune protection. It remains imperative to understand how Omicron evolves into different subvariants and causes immune escape as this could help reevaluate the current intervention strategies mostly implemented in the clinics as emergency measures to counter the pandemic and, importantly, develop new solutions. Here, w...
Source: Viruses - April 28, 2024 Category: Virology Authors: Hailong Guo Sha Ha Jason W. Botten Kai Xu Ningyan Zhang Zhiqiang An William R. Strohl John W. Shiver Tong-Ming Fu Tags: Review Source Type: research
A Case of Thyroid Gland Abscess Caused by Brucella melitensis
Mikrobiyol Bul. 2024 Apr;58(2):217-223. doi: 10.5578/mb.20249810.ABSTRACTBrucellosis is a zoonotic disease endemic in many developing countries, including Türkiye. Among the species that are pathogenic for humans; Brucella melitensis is isolated from livestock animals like sheep and goats, Brucella abortus from cattle and Brucella suis from pigs. Laboratory diagnosis of infection caused by Brucella species with gram-negative coccobacillus morphology; can be made through characteristic culture features, serological tests and molecular methods. Brucellosis, which has a wide distribution of clinical signs and symptoms; can c...
Source: Mikrobiyoloji Bulteni - April 27, 2024 Category: Microbiology Authors: Ilgaz Kazaz Ertu ğrul Yazici İsmail Selçuk Aygar Tu ğrul Hoşbul Source Type: research
